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公开(公告)号：US07839507B2
公开(公告)日：2010-11-23
申请号：US11770443
申请日：2007-06-28
IPC分类号： G01N21/25
CPC分类号： G01N21/6428 , G01N21/274 , G01N2021/6421
摘要： Methods and systems for processing signals to minimize the effects of dye crosstalk. Deconvolution of multiplexed dye signals can include corrections for residual error determined from experimental measurements. Residual error corrections can account for reaction or assay specific factors and modify the subsequent filtering of signals. Correction values can be determined for specific dye-probe conjugates to minimize dye crosstalk and may be combined with residual error correction to further minimize errors in spectral deconvolution. Apparatus, systems, and computer-readable media can process signals and modify filters based on values obtained using the methods.
摘要翻译： 用于处理信号的方法和系统，以最小化染料串扰的影响。 多重染料信号的去卷积可以包括由实验测量确定的残差的校正。 残留误差校正可以解释反应或测定特异性因素，并修改随后的信号过滤。 可以针对特定的染料 - 探针缀合物确定校正值以最小化染料串扰，并可与残留误差校正组合以进一步最小化光谱去卷积中的误差。 装置，系统和计算机可读介质可以基于使用这些方法获得的值处理信号和修改滤波器。
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2.发明申请公开(公告)号：US20110137600A1
公开(公告)日：2011-06-09
申请号：US12875995
申请日：2010-09-03
IPC分类号： G06F19/00
CPC分类号： G01B11/002 , B01L9/523 , G01B11/26 , G01N35/00584
摘要： Systems and methods are provided that comprise calibration techniques and associated systems that identify the two-dimensional position, or other alignment or positioning, of sample wells or other calibration objects located in a sample well plate, or other surface or area of interest. In some embodiments, calibration of the plate and/or positioning and/or alignment with respect to detection optics can be performed in multiple stages for two or more dimensions.
摘要翻译： 提供了包括校准技术和相关系统的系统和方法，所述校准技术和相关系统识别位于样品阱板或其他表面或感兴趣区域中的样品阱或其它校准物体的二维位置或其他对准或定位。 在一些实施例中，相对于检测光学元件的板的校准和/或定位和/或对准可以在两个或多个维度的多个阶段中进行。
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公开(公告)号：US20080201103A1
公开(公告)日：2008-08-21
申请号：US12021032
申请日：2008-01-28
申请人： Chengyong Yang , David C. Woo
发明人： Chengyong Yang , David C. Woo
CPC分类号： G06F17/18 , G01D1/14 , G01N21/6428 , G01N2021/6439
摘要： The present teachings comprise systems and methods for calibrating the background or baseline signal in a PCR or other reaction. The background signal derived from detected emissions of sample wells can be subjected to a normalized statistical metric, and be compared to a threshold or other standard to discard outlier cycles or other extraneous data. According to various embodiments, a relative standard deviation (relativeSTD) for the background component can be generated by dividing the standard deviation by the median of differences across all wells, where the difference is defined as the difference between maximum and minimum pixel values of a well. The relativeSTD as a metric is not sensitive to machine-dependent variations in absolute signal output that can be caused by different gain settings, different LED draw currents, different optical paths, or other instrumental variations. More accurate background characterization can be achieved.
摘要翻译： 本教导包括用于在PCR或其他反应中校准背景或基线信号的系统和方法。 从检测到的样本井的排放导出的背景信号可以经受归一化的统计度量，并与阈值或其他标准进行比较以丢弃异常周期或其他无关数据。 根据各种实施例，可以通过将标准偏差除以所有孔的差异中值来产生背景分量的相对标准偏差（relativeSTD），其中差被定义为井的最大和最小像素值之间的差 。 作为度量的relativeSTD对于可能由不同的增益设置，不同的LED绘制电流，不同的光路或其他工具变化引起的绝对信号输出的机器相关变化不敏感。 可以获得更准确的背景特征。
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公开(公告)号：US09784563B2
公开(公告)日：2017-10-10
申请号：US12875995
申请日：2010-09-03
CPC分类号： G01B11/002 , B01L9/523 , G01B11/26 , G01N35/00584
摘要： Systems and methods are provided that comprise calibration techniques and associated systems that identify the two-dimensional position, or other alignment or positioning, of sample wells or other calibration objects located in a sample well plate, or other surface or area of interest. In some embodiments, calibration of the plate and/or positioning and/or alignment with respect to detection optics can be performed in multiple stages for two or more dimensions.
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5.发明申请Calibrating the Positions of a Rotating and Translating Two-Dimensional Scanner 审中-公开校准旋转和平移二维扫描仪的位置公开(公告)号：US20080243415A1
公开(公告)日：2008-10-02
申请号：US12021000
申请日：2008-01-28
IPC分类号： G01P21/00
CPC分类号： G01B11/002 , B01L9/523 , G01B11/26 , G01N35/00584
摘要： Systems and methods are provided that comprise calibration techniques and associated systems that identify the two-dimensional position, or other alignment or positioning, of sample wells or other calibration objects located in a sample well plate, or other surface or area of interest. In some embodiments, calibration of the plate and/or positioning and/or alignment with respect to detection optics can be performed in multiple stages for two or more dimensions.
摘要翻译： 提供了包括校准技术和相关系统的系统和方法，所述校准技术和相关系统识别位于样品阱板或其他表面或感兴趣区域中的样品阱或其它校准物体的二维位置或其他对准或定位。 在一些实施例中，相对于检测光学元件的板的校准和/或定位和/或对准可以在两个或多个维度的多个阶段中进行。
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公开(公告)号：US07398171B2
公开(公告)日：2008-07-08
申请号：US11428048
申请日：2006-06-30
摘要： Aspects of the present invention describe a method and apparatus for automating quality control for gene expression data. A computer based device receives gene expression data associated with a spectral species and genetic sample in each well of a plate. Gene expression data may be received from a sequence detection instrument performing one or more gene expression related operations for each of the wells of the plate. The computer based device identifies gene expression data determined to have anomalous characteristics according to a set of one or more quality control metrics and may conditionally flag one or more wells of the plate affected by the anomalous characteristics. Filters can then be selectively applied to temporarily or permanently remove the flagged data from subsequent gene expression studies.
摘要翻译： 本发明的方面描述了用于使基因表达数据的质量控制自动化的方法和装置。 基于计算机的设备接收与板的每个孔中的光谱种类和遗传样品相关的基因表达数据。 基因表达数据可以从对板的每个孔执行一个或多个基因表达相关操作的序列检测仪器接收。 基于计算机的设备根据一组或多个质量控制度量来确定确定具有异常特征的基因表达数据，并且可以有条件地标记受异常特征影响的板的一个或多个孔。 然后可以选择性地施加过滤器以暂时或永久性地将标记的数据从随后的基因表达研究中去除。
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