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公开(公告)号：US07839507B2
公开(公告)日：2010-11-23
申请号：US11770443
申请日：2007-06-28
IPC分类号： G01N21/25
CPC分类号： G01N21/6428 , G01N21/274 , G01N2021/6421
摘要： Methods and systems for processing signals to minimize the effects of dye crosstalk. Deconvolution of multiplexed dye signals can include corrections for residual error determined from experimental measurements. Residual error corrections can account for reaction or assay specific factors and modify the subsequent filtering of signals. Correction values can be determined for specific dye-probe conjugates to minimize dye crosstalk and may be combined with residual error correction to further minimize errors in spectral deconvolution. Apparatus, systems, and computer-readable media can process signals and modify filters based on values obtained using the methods.
摘要翻译： 用于处理信号的方法和系统，以最小化染料串扰的影响。 多重染料信号的去卷积可以包括由实验测量确定的残差的校正。 残留误差校正可以解释反应或测定特异性因素，并修改随后的信号过滤。 可以针对特定的染料 - 探针缀合物确定校正值以最小化染料串扰，并可与残留误差校正组合以进一步最小化光谱去卷积中的误差。 装置，系统和计算机可读介质可以基于使用这些方法获得的值处理信号和修改滤波器。
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2.发明授权公开(公告)号：US08005628B2
公开(公告)日：2011-08-23
申请号：US12022087
申请日：2008-01-29
申请人： Stephen J. Gunstream
发明人： Stephen J. Gunstream
IPC分类号： G06F19/00
CPC分类号： C12Q1/6851 , G01N21/274 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G01N2021/6441 , G01N2201/12 , G01N2201/12746 , G06F19/22 , C12Q2545/113 , C12Q2545/101
摘要： Systems and methods for normalizing detected emission data collected in real-time polymerase chain reaction (RT-PCR) and other reactions, are provided. In some embodiments, a sample plate can be loaded with a fluorescent dye and subjected to a real-time PCR reaction. During the initial cycles, detected emissions that correspond to the background signal contributed by the plate, buffer, and other non-reactant pieces of the reaction system and chemistry can be identified. The raw emission data can be normalized by dividing the emission data by the identified baseline signal. According to various embodiments, the normalized amplification profile can normalize to an initial value of 1, because the actual signal emerges from the baseline at the point exponential growth begins. A normalized amplification profile based on a ratio to the baseline can create a more uniformly scaled amplification curve across different samples, filters, wells, dyes, or machines.
摘要翻译： 提供了在实时聚合酶链反应（RT-PCR）和其他反应中收集的检测到的排放数据归一化的系统和方法。 在一些实施方案中，样品板可以装载荧光染料并进行实时PCR反应。 在初始循环期间，可以鉴定出与反应体系和化学物质的板，缓冲液和其它非反应物片段所贡献的背景信号相对应的检测到的排放物。 可以通过将发射数据除以所识别的基线信号来对原始发射数据进行归一化。 根据各种实施例，归一化放大曲线可以归一化为初始值1，因为实际信号在指数增长点开始从基线出现。 基于与基线的比率的归一化扩增曲线可以在不同样品，滤器，孔，染料或机器上产生更均匀的缩放扩增曲线。
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公开(公告)号：US08945481B1
公开(公告)日：2015-02-03
申请号：US12145333
申请日：2008-06-24
申请人： Mark F. Oldham , Kenneth J. Livak , Jason E. Babcoke , H. Pin Kao , Stephen J. Gunstream , Kevin S. Bodner , Douglas P. Greiner , Nigel P. Beard , Dar Bahatt
发明人： Mark F. Oldham , Kenneth J. Livak , Jason E. Babcoke , H. Pin Kao , Stephen J. Gunstream , Kevin S. Bodner , Douglas P. Greiner , Nigel P. Beard , Dar Bahatt
IPC分类号： B01L3/00
CPC分类号： B01J19/0046 , B01L3/502715 , B01L3/502738 , B01L7/52 , B01L2200/027 , B01L2200/10 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0867 , B01L2300/185 , B01L2400/0655 , G01N21/17 , G01N2201/0407
摘要： Exemplary embodiments provide microfludic devices and methods for their use. The microfluidic device can include an array of M×N reaction sites formed by intersecting a first and second plurality of fluid channels of a flow layer. The flow layer can have a matrix design and/or a blind channel design to analyze a large number of samples under a limited number of conditions. The microfluidic device can also include a control layer including a valve system for regulating solution flow through fluid channels. In addition, by aligning the control layer with the fluid channels, the detection of the microfluidic devices, e.g., optical signal collection, can be improved by piping lights to/from the reaction sites. In an exemplary embodiment, guard channels can be included in the microfluidic device for thermal cycling and/or reducing evaporation from the reaction sites.
摘要翻译： 示例性实施例提供了用于它们的微流体装置和方法。 微流体装置可以包括通过与流动层的第一和第二多个流体通道相交形成的M×N反应位点的阵列。 流层可以具有矩阵设计和/或盲通道设计，以在有限数量的条件下分析大量样品。 微流体装置还可以包括控制层，其包括用于调节通过流体通道的溶液流动的阀门系统。 此外，通过使控制层与流体通道对准，可以通过向/从反应位置的管道灯来改善微流体装置的检测，例如光学信号收集。 在示例性实施例中，保护通道可以包括在微流体装置中用于热循环和/或减少从反应位置的蒸发。
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公开(公告)号：US07875425B2
公开(公告)日：2011-01-25
申请号：US11270841
申请日：2005-11-09
CPC分类号： B01L7/52 , B01L2200/147 , B01L2200/148 , B01L2300/0829 , C12Q1/6851 , C12Q2561/113 , C12Q2527/101
摘要： A method for calibrating temperature can include cycling temperatures of a set of wells, wherein each well of the set comprises a sample with a spectrally distinguishable species. The method can further include measuring a signal from the spectrally distinguishable species for each well at a temperature during a first temperature cycle, and calibrating the temperatures for measuring the signal from each well during subsequent temperature cycles.
摘要翻译： 用于校准温度的方法可以包括一组孔的循环温度，其中该组的每个孔包含具有光谱可区分物种的样品。 该方法还可以包括在第一温度循环期间的温度下测量来自每个孔的光谱可区分物质的信号，并且在随后的温度循环期间校准用于测量来自每个孔的信号的温度。
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公开(公告)号：US07435602B2
公开(公告)日：2008-10-14
申请号：US10757903
申请日：2004-01-14
CPC分类号： G01N21/31 , G01N21/253 , G01N21/276 , Y10T436/10 , Y10T436/11 , Y10T436/113332 , Y10T436/143333
摘要： Method and system providing calibration of light detected from biological samples with a correction factor including components for each of a plurality of spectrally distinguishable species and/or for each well and/or for each filter.
摘要翻译： 方法和系统提供从生物样品检测到的光的校准，其校正因子包括多个光谱可区分物种和/或每个孔和/或每个过滤器中的每一个的分量。
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公开(公告)号：US08285492B2
公开(公告)日：2012-10-09
申请号：US12534742
申请日：2009-08-03
申请人： Stephen J. Gunstream
发明人： Stephen J. Gunstream
CPC分类号： C12Q1/6851 , G01N21/274 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G01N2021/6441 , G01N2201/12 , G01N2201/12746 , G06F19/22 , C12Q2545/113 , C12Q2545/101
摘要： Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (PCR), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.
摘要翻译： 提供了系统和方法，用于校准在聚合酶链式反应（PCR），扩增反应，测定，过程或其他反应期间收集的排放数据或其他信息信号。 可以在单个循环或运行期间或在多次反应运行期间实现多个可检测材料的校准。 来自样品支架的每个孔，容器或其他支撑区域的读数不必采取。 内插可用于确定未使用或未知的使用检测到的发射数据或其他检测到的信息信号的发射数据或其他信息信号的值。 通过校准检测到的发射数据和内插数据，可以获得更准确的发射数据或信息信号读数。
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公开(公告)号：US20100198525A1
公开(公告)日：2010-08-05
申请号：US12534742
申请日：2009-08-03
申请人： Stephen J. Gunstream
发明人： Stephen J. Gunstream
CPC分类号： C12Q1/6851 , G01N21/274 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G01N2021/6441 , G01N2201/12 , G01N2201/12746 , G06F19/22 , C12Q2545/113 , C12Q2545/101
摘要： Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (PCR), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.
摘要翻译： 提供了系统和方法，用于校准在聚合酶链式反应（PCR），扩增反应，测定，过程或其他反应期间收集的排放数据或其他信息信号。 可以在单个循环或运行期间或在多次反应运行期间实现多个可检测材料的校准。 来自样品支架的每个孔，容器或其他支撑区域的读数不必采取。 内插可用于确定未使用或未知的使用检测到的发射数据或其他检测到的信息信号的发射数据或其他信息信号的值。 通过校准检测到的发射数据和内插数据，可以获得更准确的发射数据或信息信号读数。
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公开(公告)号：US07446288B2
公开(公告)日：2008-11-04
申请号：US11417756
申请日：2006-05-04
申请人： Steven J. Boege , Mark F. Oldham , Marc Haberstroh , Stephen J. Gunstream , Adrian Fawcett , Douglas W. Grunewald
发明人： Steven J. Boege , Mark F. Oldham , Marc Haberstroh , Stephen J. Gunstream , Adrian Fawcett , Douglas W. Grunewald
CPC分类号： H05B6/108 , B01F13/0818 , B01L3/50851 , B01L2300/0803 , B01L2300/0809 , B01L2300/1816 , H05B6/109 , H05B2214/04 , Y10T137/6606
摘要： A device is provided that comprises one or more fluid retainment regions each having at least one wall, and one or more loops in heat-transfer communication with the at least one wall. Each of the loops can comprise an electrical conductor that surrounds the same or a different fluid retainment region. A device is provided that comprises one or more fluid retainment regions each having particulates disposed therein. A system is provided that includes a platen adapted to hold a device including fluid retainment regions and one or more electrical conductors in heat-transfer communication with the fluid retainment regions. Methods of heating a sample are also provided.
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公开(公告)号：US20080182263A1
公开(公告)日：2008-07-31
申请号：US12022079
申请日：2008-01-29
申请人： Stephen J. Gunstream
发明人： Stephen J. Gunstream
CPC分类号： C12Q1/6851 , G01N21/274 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G01N2021/6441 , G01N2201/12 , G01N2201/12746 , G16B30/00 , C12Q2545/113 , C12Q2545/101
摘要： The present teachings relate to a method of generating calibration information during a real-time polymerase chain reaction (RT-PCR) or other amplification reaction. A sample well plate or other support can contain one or more dyes or other reference materials that are subjected to the same RT-PCR thermal cycles or other conditions used to conduct amplification or other reactions on a biological sample. A set of maxima values and a set of minimum values, and/or other calibration information useful for adjusting emission data for sample dyes can be recorded, for example, for 10 cycles, 20 cycles, or each cycle of a complete RT-PCR run. Such testing of dye response under realistic operating conditions can enable more accurate characterization of plate, dye, filter, or instrument response and therefore more accurate calibration corrections and other and/or adjustments.
摘要翻译： 本教导涉及在实时聚合酶链反应（RT-PCR）或其他扩增反应期间产生校准信息的方法。 样品孔板或其它载体可以含有一种或多种经受相同RT-PCR热循环或用于在生物样品上进行扩增或其他反应的其他条件的染料或其它参考物质。 可以记录一组最大值和一组最小值，和/或可用于调整样品染料的发射数据的其他校准信息，例如10个循环，20个循环或完整RT-PCR运行的每个循环 。 在真实的操作条件下对染料响应的这种测试可以实现板，染料，过滤器或仪器响应的更精确的表征，因此可以进行更精确的校准校正和其他和/或调整。
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公开(公告)号：US20080178653A1
公开(公告)日：2008-07-31
申请号：US12022094
申请日：2008-01-29
申请人： Stephen J. Gunstream
发明人： Stephen J. Gunstream
IPC分类号： G01N37/00
CPC分类号： C12Q1/6851 , G01N21/274 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G01N2021/6441 , G01N2201/12 , G01N2201/12746 , G16B30/00 , C12Q2545/113 , C12Q2545/101
摘要： Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (PCR), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.
摘要翻译： 提供了系统和方法，用于校准在聚合酶链式反应（PCR），扩增反应，测定，过程或其他反应期间收集的排放数据或其他信息信号。 可以在单个循环或运行期间或在多次反应运行期间实现多个可检测材料的校准。 来自样品支架的每个孔，容器或其他支撑区域的读数不必采取。 内插可用于确定未使用或未知的使用检测到的发射数据或其他检测到的信息信号的发射数据或其他信息信号的值。 通过校准检测到的发射数据和内插数据，可以获得更准确的发射数据或信息信号读数。
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