公开(公告)号：US20130143264A1

公开(公告)日：2013-06-06

申请号：US13536383

申请日：2012-06-28

申请人： Mermod Nicolas ,  Pierre Alain Girod ,  Philipp Bucher ,  Duc-Quang Nguyen ,  David Calabrese ,  Damien Saugy ,  Stefania Puttini

发明人： Mermod Nicolas ,  Pierre Alain Girod ,  Philipp Bucher ,  Duc-Quang Nguyen ,  David Calabrese ,  Damien Saugy ,  Stefania Puttini

IPC分类号： C12P21/00 ,  G06F19/12

CPC分类号： C12P21/00 ,  C12N15/1089 ,  C12N15/11 ,  C12N15/63 ,  G06F19/12 ,  G06F19/16 ,  G06F19/22

摘要： The present invention relates to purified and isolated DNA sequences having protein production increasing activity and more specifically to the use of matrix attachment regions (MARs) for increasing protein production activity in a eukaryotic cell. Also disclosed is a method for the identification of said active regions, in particular MAR nucleotide sequences, and the use of these characterized active MAR sequences in a new multiple transfection method.

摘要翻译： 本发明涉及具有蛋白质生产增加活性的纯化和分离的DNA序列，更具体地涉及用于增加真核细胞中蛋白质生产活性的基质附着区域（MARs）的使用。 还公开了用于鉴定所述活性区域，特别是MAR核苷酸序列的方法，以及这些表征的活性MAR序列在新的多重转染方法中的用途。

公开(公告)号：US20070178469A1

公开(公告)日：2007-08-02

申请号：US10595495

申请日：2004-10-22

申请人： Nicolas Mermod ,  Pierre Alain Girod ,  Philipp Bucher ,  Duc-Quang Nguyen ,  David Calabrese ,  Damien Saugy ,  Stefania Puttini

发明人： Nicolas Mermod ,  Pierre Alain Girod ,  Philipp Bucher ,  Duc-Quang Nguyen ,  David Calabrese ,  Damien Saugy ,  Stefania Puttini

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12P21/00 ,  C12N15/1089 ,  C12N15/11 ,  C12N15/63 ,  G06F19/12 ,  G06F19/16 ,  G06F19/22

摘要： The present invention relates to purified and isolated DNA sequences having protein production increasing activity and more specifically to the use of matrix attachment regions (MARs) for increasing protein production activity in a eukaryotic cell. Also disclosed is a method for the identification of said active regions, in particular MAR nucleotide sequences, and the use of these characterized active MAR sequences in a new multiple transfection method.

摘要翻译： 本发明涉及具有蛋白质生产增加活性的纯化和分离的DNA序列，更具体地涉及用于增加真核细胞中蛋白质生产活性的基质附着区域（MARs）的使用。 还公开了用于鉴定所述活性区域，特别是MAR核苷酸序列的方法，以及这些表征的活性MAR序列在新的多重转染方法中的用途。

公开(公告)号：US20050277942A1

公开(公告)日：2005-12-15

申请号：US10855803

申请日：2004-05-27

申请人： Karen Kullas ,  Daniel Hass ,  Thomas Capuzziello ,  Dennis Coffey ,  David Calabrese

发明人： Karen Kullas ,  Daniel Hass ,  Thomas Capuzziello ,  Dennis Coffey ,  David Calabrese

IPC分类号： A61F2/00 ,  A61F2/02

CPC分类号： A61F2/0063 ,  A61F2002/0072

摘要： An introducer and method for delivering a prosthetic fabric into a patient. The introducer includes a delivery tube having a distal end adapted to be inserted into the patient and a lumen that receives the prosthetic fabric. A loader may be provided to reconfigure the prosthetic fabric from a first configuration to a collapsed configuration and/or to insert the collapsed fabric into the lumen of the delivery tube. A plunger may be provided to push the prosthetic fabric through the delivery tube and into the patient. The loader and plunger may be combined in a dual-purpose component that is reversible to either load the fabric into the delivery tube or push the fabric through the delivery tube.

摘要翻译： 用于将假体织物递送到患者体内的导引器和方法。 引导器包括具有适于插入患者体内的远端的输送管和容纳假体织物的内腔。 可以提供装载器以将假体织物从第一构型重新配置为折叠构型和/或将折叠的织物插入输送管的内腔。 可以提供柱塞以推动假体织物穿过输送管并进入患者体内。 装载机和柱塞可以组合成双重用途的部件，其可逆地将织物加载到输送管中或者将织物推送通过输送管。

公开(公告)号：USD389356S

公开(公告)日：1998-01-20

申请号：US55107

申请日：1996-05-29

申请人： David Calabrese

设计人： David Calabrese

公开(公告)号：US08252917B2

公开(公告)日：2012-08-28

申请号：US10595495

申请日：2004-10-22

申请人： Nicolas Mermod ,  Pierre Alain Girod ,  Philipp Bucher ,  Duc-Quang Nguyen ,  David Calabrese ,  Damien Saugy ,  Stefania Puttini

发明人： Nicolas Mermod ,  Pierre Alain Girod ,  Philipp Bucher ,  Duc-Quang Nguyen ,  David Calabrese ,  Damien Saugy ,  Stefania Puttini

IPC分类号： C07H21/04 ,  C12N15/63 ,  C12N15/85 ,  C12N15/09

CPC分类号： C12P21/00 ,  C12N15/1089 ,  C12N15/11 ,  C12N15/63 ,  G06F19/12 ,  G06F19/16 ,  G06F19/22

摘要： The present invention relates to purified and isolated DNA sequences having protein production increasing activity and more specifically to the use of matrix attachment regions (MARs) for increasing protein production activity in a eukaryotic cell. Also disclosed is a method for the identification of said active regions, in particular MAR nucleotide sequences, and the use of these characterized active MAR sequences in a new multiple transfection method.

摘要翻译： 本发明涉及具有蛋白质生产增加活性的纯化和分离的DNA序列，更具体地涉及用于增加真核细胞中蛋白质生产活性的基质附着区域（MARs）的使用。 还公开了用于鉴定所述活性区域，特别是MAR核苷酸序列的方法，以及这些表征的活性MAR序列在新的多重转染方法中的用途。

公开(公告)号：US20110061117A1

公开(公告)日：2011-03-10

申请号：US12438175

申请日：2007-08-22

申请人： Nicolas Mermod ,  Pierre Alain Girod ,  David Calabrese ,  Alexandre Regamey ,  Saline Doninelli-Arope

发明人： Nicolas Mermod ,  Pierre Alain Girod ,  David Calabrese ,  Alexandre Regamey ,  Saline Doninelli-Arope

IPC分类号： A01K67/00 ,  C12N15/63 ,  C12P21/00 ,  C07H21/00 ,  C12Q1/68 ,  C12N5/00 ,  C12N15/00

CPC分类号： C12N15/63

摘要： Isolated and purified MAR sequences of human and non-human animal origin are disclosed as are nucleotide sequences corresponding to or based on them. In particular, MARs and MAR constructs with high transcription and/or protein production enhancing activities are disclosed and so are methods for identifying such MARs, designing such MAR constructs and employing them, e.g., for high yield production of proteins.

摘要翻译： 人和非人动物来源的分离和纯化的MAR序列被公开为对应于或基于它们的核苷酸序列。 特别地，公开了具有高转录和/或蛋白质生产增强活性的MAR和MAR构建体，鉴定这种MAR的方法也是如此，设计这样的MAR构建体并使用它们，例如用于高产量生产蛋白质。

公开(公告)号：US10140776B2

公开(公告)日：2018-11-27

申请号：US15181250

申请日：2016-06-13

申请人： Julia Schwarz ,  Bharat Ahluwalia ,  David Calabrese ,  Robert C J Pengelly ,  Yasaman Sheri ,  James Tichenor

发明人： Julia Schwarz ,  Bharat Ahluwalia ,  David Calabrese ,  Robert C J Pengelly ,  Yasaman Sheri ,  James Tichenor

IPC分类号： G06T19/20 ,  G06F3/01 ,  G06T19/00 ,  G06F3/0484 ,  G06T15/60

摘要： Altering properties of rendered objects and/or mixed reality environments utilizing control points associated with the rendered objects and/or mixed reality environments is described. Techniques described can include detecting a gesture performed by or in association with a control object. Based at least in part on detecting the gesture, techniques described can identify a target control point that is associated with a rendered object and/or a mixed reality environment. As the control object moves within the mixed reality environment, the target control point can track the movement of the control object. Based at least in part on the movement of the control object, a property of the rendered object and/or the mixed reality environment can be altered. A rendering of the rendered object and/or the mixed reality environment can be modified to reflect any alterations to the property.

公开(公告)号：US09879297B2

公开(公告)日：2018-01-30

申请号：US13536383

申请日：2012-06-28

申请人： Mermod Nicolas ,  Pierre Alain Girod ,  Philipp Bucher ,  Duc-Quang Nguyen ,  David Calabrese ,  Damien Saugy ,  Stefania Puttini

发明人： Mermod Nicolas ,  Pierre Alain Girod ,  Philipp Bucher ,  Duc-Quang Nguyen ,  David Calabrese ,  Damien Saugy ,  Stefania Puttini

IPC分类号： C12P21/00 ,  C12N15/09 ,  C12N15/10 ,  C12N15/11 ,  C12N15/63 ,  G06F19/12 ,  G06F19/16 ,  G06F19/22

CPC分类号： C12P21/00 ,  C12N15/1089 ,  C12N15/11 ,  C12N15/63 ,  G06F19/12 ,  G06F19/16 ,  G06F19/22

摘要： The present invention relates to purified and isolated DNA sequences having protein production increasing activity and more specifically to the use of matrix attachment regions (MARs) for increasing protein production activity in a eukaryotic cell. Also disclosed is a method for the identification of said active regions, in particular MAR nucleotide sequences, and the use of these characterized active MAR sequences in a new multiple transfection method.

公开(公告)号：US20170358144A1

公开(公告)日：2017-12-14

申请号：US15181250

申请日：2016-06-13

申请人： Julia Schwarz ,  Bharat Ahluwalia ,  David Calabrese ,  Robert CJ Pengelly ,  Yasaman Sheri ,  James Tichenor

发明人： Julia Schwarz ,  Bharat Ahluwalia ,  David Calabrese ,  Robert CJ Pengelly ,  Yasaman Sheri ,  James Tichenor

IPC分类号： G06T19/20 ,  G06F3/01 ,  G06T19/00

CPC分类号： G06T19/20 ,  G06F3/011 ,  G06F3/013 ,  G06F3/017 ,  G06F3/04842 ,  G06F3/04845 ,  G06T15/60 ,  G06T19/006 ,  G06T2219/20 ,  G06T2219/2004 ,  G06T2219/2016

摘要： Altering properties of rendered objects and/or mixed reality environments utilizing control points associated with the rendered objects and/or mixed reality environments is described. Techniques described can include detecting a gesture performed by or in association with a control object. Based at least in part on detecting the gesture, techniques described can identify a target control point that is associated with a rendered object and/or a mixed reality environment. As the control object moves within the mixed reality environment, the target control point can track the movement of the control object. Based at least in part on the movement of the control object, a property of the rendered object and/or the mixed reality environment can be altered. A rendering of the rendered object and/or the mixed reality environment can be modified to reflect any alterations to the property.

公开(公告)号：US20120231449A1

公开(公告)日：2012-09-13

申请号：US13496817

申请日：2010-09-20

申请人： Nicolas Mermod ,  Pierre-Alain Girod ,  Mélanie Grandjean ,  Valérie Le Fourn ,  David Calabrese ,  Alexandre Regamey

发明人： Nicolas Mermod ,  Pierre-Alain Girod ,  Mélanie Grandjean ,  Valérie Le Fourn ,  David Calabrese ,  Alexandre Regamey

IPC分类号： C12N15/85 ,  C12Q1/68 ,  C12N5/10

CPC分类号： C12N15/907 ,  C12N15/85 ,  C12N2830/46

摘要： Disclosed are methods and eukaryotic host cells for transgene expression. The cells may be treated and/or modified to increase homologous recombination (HR), decrease non homologous end joining (NHEJ) and/or to enhance a HR/NHEJ ratio in said cell. Such cells can be transfected with vectors comprising the transgene, which advantageously integrates into the genome of the cell to form a concatemeric structure which may comprise more than 200 transgene copies. Certain expression enhancing elements such as MARs are advantageously provided to further enhance and/or facilitate transgene expression. Disclosed is also a recombinant eukaryotic host cell, in particular a non-primate host cell, comprising a transgenic sequence encoding a protein and/or a RNA, in particular a primate protein and/or RNA, involved in translocation across the ER membrane and/or secretion across the cytoplasmic membrane.

摘要翻译： 公开了用于转基因表达的方法和真核宿主细胞。 可以治疗和/或修饰细胞以增加同源重组（HR），减少非同源末端连接（NHEJ）和/或增强所述细胞中的HR / NHEJ比率。 可以用包含转基因的载体转染这样的细胞，其有利地整合到细胞的基因组中以形成可以包含超过200个转基因拷贝的并联结构。 有利地提供某些表达增强元件如MARs以进一步增强和/或促进转基因表达。 还公开了重组真核宿主细胞，特别是非灵长类动物宿主细胞，其包含编码蛋白质和/或RNA的转基因序列，特别是涉及穿过ER膜的易位蛋白和/或RNA，和/ 或分泌跨细胞质膜。