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公开(公告)号:US5616459A
公开(公告)日:1997-04-01
申请号:US931560
申请日:1992-08-18
申请人: Fred R. Kramer , David Dubnau , Karl A. Drlica , Abraham Pinter
发明人: Fred R. Kramer , David Dubnau , Karl A. Drlica , Abraham Pinter
CPC分类号: C12N15/113 , C12N15/1041 , C12N9/1007 , C12Q1/6811 , C12N2310/121
摘要: Mutant ribozymes are screened by culturing cells whose survival is dependant upon cleavage of RNA by a ribozyme, which cleavage causes the cells to survive in the presence of an agent which otherwise would kill the cells, and selecting cells which survive.
摘要翻译: 通过培养其生存依赖于核酶裂解RNA的细胞来筛选突变核酶,该细胞裂解导致细胞在另外会杀死细胞的试剂存在下存活,并选择存活的细胞。