公开(公告)号：US11913138B2

公开(公告)日：2024-02-27

申请号：US17405172

申请日：2021-08-18

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY ,  THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS

Inventor： Joshua Labaer ,  Kevin Gorman ,  Brian Kay ,  Jie Wang ,  Ji Qiu

IPC: C40B30/04 ,  C12N15/10 ,  C12Q1/6811

CPC classification number: C40B30/04 ,  C12N15/1037 ,  C12N15/1041 ,  C12N15/1062 ,  C12Q1/6811 ,  C12Q1/6811 ,  C12Q2565/501 ,  C12Q2565/515

Abstract: Methods for screening of affinity reagents for many target proteins of interest simultaneously. Arrayed targets (e.g., peptide, protein, RNA, cell, etc.) are used in affinity selection experiments to reduce the amount of target needed and to improve the throughput of discovering recombinant affinity reagents to a large collection of targets.

公开(公告)号：US10011830B2

公开(公告)日：2018-07-03

申请号：US14890864

申请日：2014-05-15

Applicant: THE BOARD OF TRUSTEES OF THE LELAND

Inventor： William J. Greenleaf ,  Curtis J. Layton

IPC: C12N15/10 ,  C40B50/06 ,  C40B50/08 ,  C40B30/04

CPC classification number: C12N15/1041 ,  C12N15/1058 ,  C12N15/1062 ,  C12N15/1075 ,  C40B30/04 ,  C40B50/06 ,  C40B50/08

Abstract: A novel method for displaying proteins and peptides is disclosed in which individual proteins or peptides remain associated with the DNA encoding them. Proteins or peptides can be generated by in vitro translation of DNA templates, either free in solution or arrayed on a solid support, such that the proteins or peptides remain immobilized on their DNA templates. In particular, high throughput sequencing can be combined with high throughput functional characterization of encoded proteins and peptides, wherein the identity of each protein or peptide is determined by DNA sequencing, and functional studies are carried out directly on each protein or peptide while immobilized on the DNA template encoding it. The methods of the invention should find numerous applications, for example, in high throughput genetic or pharmacological screening, epitope mapping, and protein engineering and directed evolution.

公开(公告)号：US20170306039A1

公开(公告)日：2017-10-26

申请号：US15520639

申请日：2014-10-22

Applicant: Crescendo Biologics Limited

Inventor： Bryan Edwards ,  Mingyue He

IPC: C07K16/28 ,  C07K16/46 ,  C12N15/10 ,  C07K16/00 ,  C07K16/24

CPC classification number: C07K16/2878 ,  C07K16/00 ,  C07K16/005 ,  C07K16/18 ,  C07K16/241 ,  C07K16/46 ,  C07K2317/21 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/569 ,  C07K2317/76 ,  C07K2317/94 ,  C07K2318/10 ,  C12N15/1041

Abstract: The invention provides human VH scaffold sequences, libraries derived therefrom and methods of producing. The scaffolds have high expression, solubility and are functional.

公开(公告)号：US09708602B2

公开(公告)日：2017-07-18

申请号：US14894262

申请日：2014-07-09

Applicant: Universität Konstanz

Inventor： Rainer Nikolay ,  Elke Deuerling

IPC: A61K38/00 ,  C12Q1/18 ,  C12N15/62 ,  C12N15/10 ,  C07K14/195 ,  G01N33/542 ,  C07K14/435

CPC classification number: C12N15/1041 ,  C07K14/195 ,  C07K14/43595 ,  C07K2319/60 ,  C12Q1/18 ,  G01N33/542

Abstract: The invention relates to a method and a prokaryotic cell for identifying a compound, which interferes with ribosome biogenesis, assembly and/or degradation. The cell expresses a first fusion protein comprising a first ribosomal protein, an amino acid linker and a first fluorescent protein, and a second fusion protein comprising a second ribosomal protein, an amino acid linker and a second fluorescent protein. The invention further relates to a gene construct comprising a first element encoding a first ribosomal protein fused to a first fluorescent protein by an amino acid linker, and a second element encoding a second ribosomal protein fused to a second fluorescent protein by an amino acid linker.

公开(公告)号：US09284547B2

公开(公告)日：2016-03-15

申请号：US13867848

申请日：2013-04-22

Applicant: The Regents of the University of California

Inventor： Jonathan Weissman ,  Nicholas Ingolia ,  Sina Ghaemmaghami ,  John Newman

IPC: C12Q1/68 ,  C12N15/10 ,  C40B40/08 ,  C40B50/06

CPC classification number: C12N15/1041 ,  C12N15/1034 ,  C12N15/1093 ,  C12Q1/6809 ,  C40B40/08 ,  C40B50/06

Abstract: Methods are provided for, inter alia, detecting nucleic acid molecules resistant to degradation, such as a plurality of RNA molecules bound to a ribosome, using various technologies including deep sequencing.

公开(公告)号：US09279122B2

公开(公告)日：2016-03-08

申请号：US14248919

申请日：2014-04-09

Applicant: ADAPTIMMUNE LIMITED ,  IMMUNOCORE LIMITED

Inventor： Bent Karsten Jakobsen ,  Peter Eamon Molloy ,  Yi Li

IPC: C40B40/10 ,  C12N15/10 ,  C07K14/725 ,  C12N7/00 ,  C40B40/02 ,  A61K38/00

CPC classification number: C12N15/1037 ,  A61K38/00 ,  C07K14/7051 ,  C12N7/00 ,  C12N15/1041 ,  C12N15/1058 ,  C12N2795/18121 ,  C12N2795/18143 ,  C40B40/02 ,  C40B40/10

Abstract: A proteinaceous particle, for example a bacteriophage, ribosome or cell, displaying on its surface a T-cell receptor (TCR). The displayed TCR is preferably a heterodimer having a non-native disulfide bond between constant domain residues. Such display particles may be used for the creation of diverse TCR libraries for the identification of high affinity TCRs. Several high affinities are disclosed.

公开(公告)号：US09133452B2

公开(公告)日：2015-09-15

申请号：US13806470

申请日：2011-06-23

Applicant: Takuya Ueda ,  Takashi Kanamori ,  Kanehisa Kojoh ,  Shizue Katoh ,  Akira Miyakoshi

Inventor： Takuya Ueda ,  Takashi Kanamori ,  Kanehisa Kojoh ,  Shizue Katoh ,  Akira Miyakoshi

IPC: C12N15/10 ,  C12N15/67

CPC classification number: C12N15/1065 ,  C12N15/1041 ,  C12N15/1093 ,  C12N15/67

Abstract: The present invention provides a method of producing a maturated oligonucleotide library, including a step of obtaining a terminal-modified product of a maturation target oligonucleotide library, including adding a tag sequence to the 5′ terminus of the maturation target oligonucleotide library and an arrest sequence, which stalls translation elongation on a ribosome, to the 3′ terminus of the maturation target oligonucleotide library, a step of transcribing the terminal-modified sequence product to give a transcript, and a step of in vitro translation for translating the transcript in vitro, wherein the maturation target oligonucleotide library is a random oligonucleotide library.

Abstract translation: 本发明提供一种生产成熟寡核苷酸文库的方法，包括获得成熟靶寡核苷酸文库的末端修饰产物的步骤，包括向成熟靶寡核苷酸文库的5'末端加入标签序列， ，其将核糖体上的翻译延伸失活到成熟靶寡核苷酸文库的3'末端，转录末端修饰的序列产物以产生转录物的步骤，以及用于体外翻译转录物的体外翻译步骤， 其中成熟靶寡核苷酸文库是随机寡核苷酸文库。

公开(公告)号：US20140349855A1

公开(公告)日：2014-11-27

申请号：US14249904

申请日：2014-04-10

Applicant: ADAPTIMMUNE LIMITED ,  IMMUNOCORE LIMITED

Inventor： Bent Karsten Jakobsen ,  Torben Bent Andersen ,  Peter Eamon Molloy ,  Yi Li

IPC: C07K14/725 ,  C12N15/10

CPC classification number: C12N15/1037 ,  A61K38/00 ,  C07K14/7051 ,  C12N7/00 ,  C12N15/1041 ,  C12N15/1058 ,  C12N2795/18121 ,  C12N2795/18143 ,  C40B40/02 ,  C40B40/10

Abstract: A proteinaceous particle, for example a bacteriophage, ribosome or cell, displaying on its surface a T-cell receptor (TCR). The displayed TCR is preferably a heterodimer having a non-native disulfide bond between constant domain residues. Such display particles may be used for the creation of diverse TCR libraries for the identification of high affinity TCRs. Several high affinities are disclosed.

公开(公告)号：US08697655B2

公开(公告)日：2014-04-15

申请号：US13608052

申请日：2012-09-10

Applicant: Akira Wada ,  Yoshihiro Ito ,  Takashi Kitajima

Inventor： Akira Wada ,  Yoshihiro Ito ,  Takashi Kitajima

IPC: A61K38/10 ,  C12N15/00 ,  C07H21/04 ,  A61K38/00 ,  C07K1/00 ,  A61K38/24

CPC classification number: G01N33/6845 ,  A61L27/227 ,  A61L27/54 ,  A61L2300/414 ,  C07K7/08 ,  C07K14/485 ,  C12N11/14 ,  C12N15/1041

Abstract: Provided is a peptide including the following amino acid sequence. Tyr-Xaa0-Xaa1-Tyr-Tyr-Xaa2-Xaa3-Tyr-Xaa4-Xaa5-Xaa6-Xaa7-Xaa8-Xaa9-Xaa10-Xaa11 (SEQ ID NO: 4: wherein Xaa0, Xaa1, Xaa2, Xaa3, Xaa4, Xaa5, Xaa6, Xaa7, Xaa8, Xaa9, Xaa10 and Xaa 11 represent any amino acid) or Tyr-Asn-Asp-Tyr-Tyr-Tyr-Tyr-Cys-Tyr-Arg-Asp-Tyr-Asp (SEQ ID NO: 20).

Abstract translation: 提供了包含以下氨基酸序列的肽。 Tyr-Xaa0-Xaa1-Tyr-Tyr-Xaa2-Xaa3-Tyr-Xaa4-Xaa5-Xaa6-Xaa7-Xaa8-Xaa9-Xaa10-Xaa11（SEQ ID NO：4：其中Xaa0，Xaa1，Xaa2，Xaa3，Xaa4，Xaa5 ，Xaa6，Xaa7，Xaa8，Xaa9，Xaa10和Xaa11表示任何氨基酸）或Tyr-Asn-Asp-Tyr-Tyr-Tyr-Tyr-Cys-Tyr-Arg-Asp-Tyr-Asp（SEQ ID NO： ）。

公开(公告)号：US20130288905A1

公开(公告)日：2013-10-31

申请号：US13867848

申请日：2013-04-22

Applicant: The Regents of the University of California

Inventor： Jonathan Weissman ,  Nicholas Ingolia ,  Sina Ghaemmaghami ,  John Newman

IPC: C12N15/10

CPC classification number: C12N15/1041 ,  C12N15/1034 ,  C12N15/1093 ,  C12Q1/6809 ,  C40B40/08 ,  C40B50/06

Abstract: Methods are provided for, inter alia, detecting nucleic acid molecules resistant to degradation, such as a plurality of RNA molecules bound to a ribosome, using various technologies including deep sequencing.

Abstract translation: 提供的方法尤其是使用包括深度测序的各种技术来检测抗降解的核酸分子，例如与核糖体结合的多个RNA分子。