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    Apparatus for continuously preparing castable metal matrix composite material
    1.
    发明授权
    Apparatus for continuously preparing castable metal matrix composite material 失效
    用于连续制备浇注金属基复合材料的设备

    公开(公告)号：US5531425A

    公开(公告)日：1996-07-02

    申请号：US192950

    申请日：1994-02-07

    Applicant: Michael D. Skibo ,  David M. Schuster ,  Richard S. Bruski

    Inventor： Michael D. Skibo ,  David M. Schuster ,  Richard S. Bruski

    IPC: B01F7/16 ,  B22D1/00 ,  B22D11/11 ,  C22C1/00 ,  C22C1/10 ,  C22C32/00 ,  F27D3/00 ,  F27D27/00 ,  B22D11/00

    CPC classification number: F27D27/00 ,  B01F7/166 ,  B22D1/00 ,  B22D11/11 ,  C22C1/005 ,  C22C1/1005 ,  C22C1/1036 ,  C22C32/0036 ,  C22C32/0063 ,  C22C2001/1047 ,  F27D3/0026

    Abstract: A method and apparatus for preparing a continuous flow of castable composite materials of nonmetallic particles in a metallic matrix, wherein particles are mixed into a molten metallic alloy to wet the molten metal to the particles, and the particles and metal are sheared past each other to promote wetting of the particles by the metal. The mixing occurs while minimizing the introduction of gas into the mixture, and while minimizing the retention of gas at the particle-liquid interface. Mixing is done at or below a maximum temperature whereat the particles do not substantially chemically degrade in the molten metal during the time required for processing, and casting is done at a temperature sufficiently high that there is no solid metal present in the melt.

    Abstract translation: 一种用于制备金属基体中的非金属颗粒的可浇注复合材料的连续流动的方法和装置，其中将颗粒混合到熔融金属合金中以将熔融金属润湿至颗粒，并且将颗粒和金属彼此相互剪切， 促进金属对颗粒的润湿。 混合发生在将气体引入混合物中最小化的同时，同时使气体在颗粒 - 液体界面处的保留最小化。 混合在等于或低于最高温度或低于处理所需时间内熔融金属中基本上不会化学降解的最高温度，并且在足够高的温度下进行铸造，使得在熔体中不存在固体金属。

    Promoter ligation activated transcription amplification of nucleic acid sequences
    2.
    发明授权
    Promoter ligation activated transcription amplification of nucleic acid sequences 失效
    促性腺激素激活转录核酸核酸序列的扩增

    公开(公告)号：US5194370A

    公开(公告)日：1993-03-16

    申请号：US524306

    申请日：1990-05-16

    Applicant: Mark S. Berninger ,  David M. Schuster ,  Ayoub Rashtchian

    Inventor： Mark S. Berninger ,  David M. Schuster ,  Ayoub Rashtchian

    IPC: C12Q1/68

    CPC classification number: C12Q1/6865 ,  C12Q1/6876 ,  C12Q2600/158 ,  Y10T436/143333

    Abstract: This invention discloses a scheme for producing nucleic acid end products that are functionally or exactly identical to the starting products, thereby resulting in exponential amplification of a desired nucleic acid sequence. Specifically, sequences are cycled between RNA and DNA forms using the following basic steps: (1) a T7 RNA polymerase promoter is ligated onto a single-stranded DNA template; (2) T7 RNA polymerase makes many copies of RNA: (3) a complementary DNA is made from the RNA by extension of a primer by reverse transcriptase; and (4) the RNA template is removed by ribonuclease H. This amplification method is useful for purposes such as genetic research and diagnostic assays.

    Abstract translation: 本发明公开了一种用于产生与起始产物功能或完全相同的核酸终产物的方案，从而导致所需核酸序列的指数扩增。 具体地，使用以下基本步骤在RNA和DNA形式之间循环序列：（1）将T7RNA聚合酶启动子连接到单链DNA模板上; （2）T7 RNA聚合酶产生许多拷贝的RNA：（3）通过逆转录酶延伸引物从RNA制备互补DNA; 和（4）通过核糖核酸酶H去除RNA模板。该扩增方法可用于遗传研究和诊断测定等目的。

    Process for preparation of composite materials containing nonmetallic particles in a metallic matrix
    3.
    发明授权
    Process for preparation of composite materials containing nonmetallic particles in a metallic matrix 失效
    在金属基质中制备含有非金属颗粒的复合材料的方法

    公开(公告)号：US4865806A

    公开(公告)日：1989-09-12

    申请号：US72122

    申请日：1987-07-09

    Applicant: Michael D. Skibo ,  David M. Schuster

    Inventor： Michael D. Skibo ,  David M. Schuster

    IPC: B22D19/14 ,  B01F7/00 ,  B01F7/16 ,  B01F15/00 ,  C22C1/00 ,  C22C1/10 ,  C22C32/00 ,  F27D3/00 ,  F27D27/00

    CPC classification number: B01F7/00641 ,  B01F7/16 ,  B01F7/166 ,  C22C1/005 ,  C22C1/1005 ,  C22C1/1036 ,  C22C32/0063 ,  F27D27/00 ,  C22C2001/1047 ,  F27D3/0026

    Abstract: A method for preparing cast composite materials of nonmetallic carbide particles in a metallic matrix, wherein the particles are roasted and then mixed into a molten metallic alloy to wet the molten metal to the particles, and the particles and metal are sheared past each other to promote wetting of the particles by the metal. The particles are roasted in air or other source of oxygen to remove the carbon from the near-surface region of the particles and to produce an oxide surface diffusion barrier, resulting in a reduction of carbide formation in the molten matrix. The mixing occurs while minimizing the introduction of gas into the mixture, and while minimizing the retention of gas at the particle-liquid interface. Mixing is done at a maximum temperature whereat the particles do not substantially chemically degrade in the molten metal during the time required for processing, and casting is done at a temperature sufficiently high that there is no solid metal present in the melt.

    Abstract translation: 一种在金属基质中制备非金属碳化物颗粒的铸造复合材料的方法，其中将颗粒焙烧，然后混合到熔融金属合金中以将熔融金属润湿至颗粒，并且将颗粒和金属彼此剪切以促进 通过金属润湿颗粒。 颗粒在空气或其它氧源中焙烧以从颗粒的近表面区域除去碳，并产生氧化物表面扩散阻挡层，导致熔融基体中碳化物形成的减少。 混合发生在将气体引入混合物中最小化的同时，同时使气体在颗粒 - 液体界面处的保留最小化。 在最高温度下进行混合，在该温度下，在加工所需的时间内，熔融金属中的颗粒基本上不会化学降解，并且在足够高的温度下进行浇铸，使得熔体中不存在固体金属。

    Use of deoxyinosine containing primers to balance primer efficiency in the amplification of nucleic acid molecules
    6.
    发明授权
    Use of deoxyinosine containing primers to balance primer efficiency in the amplification of nucleic acid molecules 失效
    使用含有脱氧蛋白的引物平衡引物在核酸分子扩增中的效率

    公开(公告)号：US5578467A

    公开(公告)日：1996-11-26

    申请号：US246921

    申请日：1994-05-20

    Applicant: David M. Schuster ,  Ayoub Rashtchian

    Inventor： David M. Schuster ,  Ayoub Rashtchian

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: C12Q1/6844 ,  C12Q1/6832 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q2600/156

    Abstract: The present invention provides improved methods for amplifying a nucleic acid molecule. Specifically, the invention involves replacing at least one nucleotide of an oligonucleotide with a deoxyinosine residue, so as to more equalize the efficiency with which that oligonucloetide and a second oligonucleotide hybridize to a target molecule, and then amplifying the target molecule using, for example, the polymerase chain reaction. Improved amplification results from the improvement in the relative hybridization efficiencies.

    Abstract translation: 本发明提供了用于扩增核酸分子的改进方法。 具体地说，本发明涉及用脱氧肌苷残基替换寡核苷酸的至少一个核苷酸，从而更均衡该寡核苷酸与第二寡核苷酸与目标分子杂交的效率，然后使用例如， 聚合酶链反应。 改进的扩增来自相对杂交效率的提高。

    Cast composite material
    8.
    发明授权
    Cast composite material 失效
    铸造复合材料

    公开(公告)号：US5167920A

    公开(公告)日：1992-12-01

    申请号：US259581

    申请日：1988-10-18

    Applicant: Michael D. Skibo ,  David M. Schuster

    Inventor： Michael D. Skibo ,  David M. Schuster

    IPC: B01F7/16 ,  C22C1/00 ,  C22C1/10 ,  F27D3/00 ,  F27D27/00

    CPC classification number: F27D27/00 ,  B01F7/166 ,  C22C1/005 ,  C22C1/1005 ,  C22C1/1036 ,  C22C2001/1047 ,  F27D3/0026 ,  Y10T428/12486

    Abstract: A method and apparatus for preparing cast composite materials of nonmetallic particles in a metallic matrix, wherein particles are mixed into a molten metallic alloy to wet the molten metal to the particles, and the particles and metal are sheared past each other to promote wetting of the particles by the metal. The mixing occurs while minimizing the introduction of gas into the mixture, and while minimizing the retention of gas at the particle-liquid interface. Mixing is done at a maximum temperature whereat the particles do not substantially chemically degrade in the molten metal during the time required for processing, and casting is done at a temperature sufficiently high that there is no solid metal present in the melt. Mixing is preferably accomplished with a dispersing impeller, or a dispersing impeller used with a sweeping impeller.

    Abstract translation: 一种用于在金属基体中制备非金属颗粒的铸造复合材料的方法和装置，其中将颗粒混合到熔融金属合金中以将熔融金属润湿至颗粒，并且将颗粒和金属彼此剪切以促进润湿 颗粒由金属制成。 混合发生在将气体引入混合物中最小化的同时，同时使气体在颗粒 - 液体界面处的保留最小化。 在最高温度下进行混合，在该温度下，在加工所需的时间内，熔融金属中的颗粒基本上不会化学降解，并且在足够高的温度下进行浇铸，使得在熔体中不存在固体金属。 混合优选用分散叶轮或与清扫叶轮一起使用的分散叶轮完成。

    Compositions and methods for cDNA synthesis
    9.
    发明授权
    Compositions and methods for cDNA synthesis 有权
    cDNA合成的组合物和方法

    公开(公告)号：US07638612B2

    公开(公告)日：2009-12-29

    申请号：US10653416

    申请日：2003-09-03

    Applicant: Ayoub Rashtchian ,  David M. Schuster

    Inventor： Ayoub Rashtchian ,  David M. Schuster

    IPC: C07H21/00 ,  C12Q1/68 ,  C12P19/34 ,  C07K14/00

    CPC classification number: C12P19/34 ,  C12N15/1096 ,  C12Q1/6846 ,  C12Q2527/143 ,  C12Q2525/179 ,  C12Q2521/107

    Abstract: Methods for making cDNA molecules, for amplification of RNA by PCR and for preparation of cDNA libraries are provided. Kits for making cDNA molecules also are provided. Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of RNA into cDNA and RT PCR without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cDNA synthesis or nucleic acid amplification (e.g., by the Polymerase Chain Reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications.

    Abstract translation: 提供了制备cDNA分子，通过PCR扩增RNA和制备cDNA文库的方法。 还提供了制备cDNA分子的试剂盒。 还提供组合物，其包括试剂的混合物，包括逆转录酶，缓冲液，辅因子和其它组分，适于立即用于将RNA转化成cDNA和RT PCR，而不进行稀释或添加其它组分。 这些组合物可单独或以试剂盒的形式用于cDNA合成或核酸扩增（例如，通过聚合酶链式反应）或在各种研究，医学，诊断，法医和农业应用中使用逆转录酶的任何方法 。

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