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公开(公告)号：US07584718B2
公开(公告)日：2009-09-08
申请号：US12229186
申请日：2008-08-20
IPC分类号： A01K29/00
CPC分类号： A01K63/04 , A01K61/10 , A01K61/80 , A01K61/85 , A23K20/142 , A23K20/147 , A23K20/20 , A23K20/22 , A23K50/80 , Y02A40/81 , Y02A40/845
摘要： The invention relates to methods for raising marine fish in freshwater. The methods involve transferring fish to freshwater containing at least one Polyvalent Cation Sensing Receptor (PVCR) modulator in an amount sufficient to increase expression and/or sensitivity of at least one PVCR; and adding feed for fish consumption to the freshwater, wherein the feed comprises an amount of NaCl sufficient to contribute to a significant increased level of the PVCR modulator in serum of the marine fish.
摘要翻译： 本发明涉及在淡水中养殖海鱼的方法。 所述方法包括将鱼转移至含有至少一种多价阳离子感应受体（PVCR）调节剂的淡水，其量足以增加至少一种PVCR的表达和/或敏感性; 并向淡水添加鱼类消费的饲料，其中饲料包含一定量的NaCl，足以有助于海鱼血清中PVCR调节剂的显着提高。
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公开(公告)号：US07121227B2
公开(公告)日：2006-10-17
申请号：US10626115
申请日：2003-07-24
IPC分类号： A01K61/02
摘要： The invention relates to methods, compositions and kits for improving the raising of pre-adult anadromous fish, or preparing pre-adult anadromous fish for transfer to seawater. The methods involve adding at least one Polyvalent Cation Sensing Receptor (PVCR) modulator to the freshwater in an amount sufficient to increase expression and/or sensitivity of at least one PVCR; and adding feed for fish consumption to the freshwater, wherein the feed comprises an amount of NaCl sufficient to contribute to a significantly increased level of the PVCR modulator in serum of the pre-adult anadromous fish.
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公开(公告)号：US06655318B2
公开(公告)日：2003-12-02
申请号：US10270876
申请日：2002-10-11
IPC分类号： A01K6100
摘要： The invention relates to methods, compositions and kits for improving the raising of pre-adult anadromous fish, or preparing pre-adult anadromous fish for transfer to seawater. The methods involve adding at least one Polyvalent Cation Sensing Receptor (PVCR) modulator to the freshwater in an amount sufficient to increase expression and/or sensitivity of at least one PVCR; and adding feed for fish consumption to the freshwater, wherein the feed comprises an amount of NaCl sufficient to contribute to a significantly increased level of the PVCR modulator in serum of the pre-adult anadromous fish.
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公开(公告)号：US06564747B2
公开(公告)日：2003-05-20
申请号：US09975553
申请日：2001-10-11
IPC分类号： A01K6100
摘要： The invention relates to methods, compositions and kits for improving the raising of pre-adult anadromous fish, or preparing pre-adult anadromous fish for transfer to seawater. The methods involve adding at least one Polyvalent Cation Sensing Receptor (PVCR) modulator to the freshwater in an amount sufficient to increase expression and/or sensitivity of at least one PVCR; and adding feed for fish consumption to the freshwater, wherein the feed comprises an amount of NaCl sufficient to contribute to a significantly increased level of the PVCR modulator in serum of the pre-adult anadromous fish.
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公开(公告)号：US06463882B1
公开(公告)日：2002-10-15
申请号：US09687373
申请日：2000-10-12
IPC分类号： A01K6100
CPC分类号： A01K63/04 , A01K61/10 , A01K61/80 , A01K61/85 , A23K20/142 , A23K20/147 , A23K20/20 , A23K20/22 , A23K50/80 , Y02A40/81 , Y02A40/845
摘要： The invention relates to methods, compositions and kits for raising marine fish in freshwater. The methods involve adding at least one Polyvalent Cation Sensing Receptor (PVCR) modulator to the freshwater in an amount sufficient to increase expression and/or sensitivity of at least one PVCR; and adding feed for fish consumption to the freshwater, wherein the feed comprises an amount of NaCl sufficient to contribute to a significant increased level of the PVCR modulator in serum of the marine fish.
摘要翻译： 本发明涉及用于在淡水中养殖海鱼的方法，组合物和试剂盒。 所述方法包括以足以增加至少一个PVCR的表达和/或敏感性的量向淡水中加入至少一种多价阳离子感应受体（PVCR）调节剂; 并向淡水添加鱼类消费的饲料，其中饲料包含一定量的NaCl，足以有助于海鱼血清中PVCR调节剂的显着提高。
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公开(公告)号：US07069876B2
公开(公告)日：2006-07-04
申请号：US10410872
申请日：2003-04-09
IPC分类号： A01K61/00
摘要： Methods, compositions and kits for improving the raising of pre-adult anadromous fish, or preparing pre-adult anadromous fish for transfer to seawater. The methods involve adding at least one Polyvalent Cation Sensing Receptor (PVCR) modulator to the freshwater in an amount sufficient to increase expression and/or sensitivity of at least one PVCR; and adding feed for fish consumption to the freshwater, wherein the feed comprises an amount of NaCl sufficient to contribute to a significantly increased level of the PVCR modulator in serum of the pre-adult anadromous fish.
摘要翻译： 用于改善成年前成鱼的饲养的方法，组合物和试剂盒，或制备用于转运到海水的成年前成鱼。 所述方法包括以足以增加至少一个PVCR的表达和/或敏感性的量向淡水中加入至少一种多价阳离子感应受体（PVCR）调节剂; 并向淡水添加鱼类消费的饲料，其中饲料包含一定量的NaCl，足以有助于在成年前成鱼的血清中PVCR调节剂的水平显着增加。
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公开(公告)号：US6140075A
公开(公告)日：2000-10-31
申请号：US902486
申请日：1997-07-29
CPC分类号： C07K14/415 , C07K16/2866 , C07K16/30 , C12N15/8221 , C12N15/8257 , C12N15/8258 , C07K2317/13 , C07K2317/622
摘要： A method for producing antibodies and plant protein toxins in plant cells is described which includes the steps of providing a genetic construct to encode the secretable protein, delivering copies of the construct into a liquid suspension culture of tobacco cells, selecting for cells that have acquired the genetic construct, and allowing the desired protein to accumulate in the liquid media in which the plant cells are grown, and isolating the desired protein away from the tobacco cells.
摘要翻译： 描述了在植物细胞中产生抗体和植物蛋白毒素的方法，其包括以下步骤：提供编码可分泌蛋白质的遗传构建体，将构建体的拷贝递送到烟草细胞的液体悬浮培养物中，选择获得 遗传构建体，并使所需的蛋白质积聚在植物细胞生长的液体培养基中，并将期望的蛋白质从烟草细胞中分离出来。
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公开(公告)号：US20090078212A1
公开(公告)日：2009-03-26
申请号：US12229186
申请日：2008-08-20
IPC分类号： A01K61/00
CPC分类号： A01K63/04 , A01K61/10 , A01K61/80 , A01K61/85 , A23K20/142 , A23K20/147 , A23K20/20 , A23K20/22 , A23K50/80 , Y02A40/81 , Y02A40/845
摘要： The invention relates to methods for raising marine fish in freshwater. The methods involve transferring fish to freshwater containing at least one Polyvalent Cation Sensing Receptor (PVCR) modulator in an amount sufficient to increase expression and/or sensitivity of at least one PVCR; and adding feed for fish consumption to the freshwater, wherein the feed comprises an amount of NaCl sufficient to contribute to a significant increased level of the PVCR modulator in serum of the marine fish.
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公开(公告)号：US06748900B2
公开(公告)日：2004-06-15
申请号：US10268051
申请日：2002-10-08
申请人： H. William Harris, Jr. , Steven Jury , David R. Russell , Jacqueline Nearing , Marlies Betka , Timothy Linley , Edward M. Brown
发明人： H. William Harris, Jr. , Steven Jury , David R. Russell , Jacqueline Nearing , Marlies Betka , Timothy Linley , Edward M. Brown
IPC分类号： A01K6100
CPC分类号： C07K14/461 , A01K79/00 , A01K79/02 , A23K50/80
摘要： The present invention relates to methods of imprinting fish in freshwater with at least one odorant for the fish and causing the imprinted fish to react to the odorant in seawater, wherein the fish are maintained in freshwater prior to transfer to seawater. The method includes: adding at least one Polyvalent Cation Sensing Receptor (PVCR) modulator to the freshwater in an amount sufficient to modulate expression and/or sensitivity of at least one PVCR, the PVCR modulator being one which alters olfactory sensing of the fish to the odorant; adding feed for fish consumption to the freshwater, the feed containing the odorant and an amount of NaCl sufficient to contribute to a significantly increased level of the PVCR modulator in serum of the fish upon consumption of the feed, whereby the fish are imprinted with the odorant; transferring the imprinted fish to seawater; and providing a source of said odorant in the seawater to which the imprinted fish are transferred, thereby causing the imprinted fish to react to said odorant. The present invention also includes methods of homing or attracting fish, as well as methods for repelling fish by modulating the expression and/or sensitivity of the PVCR in the olfactory apparatus of the fish.
摘要翻译： 本发明涉及将鱼类在淡水中印迹至少一种用于鱼类的气味剂并使印迹鱼与海水中的气味剂反应的方法，其中在转移到海水之前将鱼保持在淡水中。 该方法包括：将至少一种多价阳离子感应受体（PVCR）调节剂以足以调节至少一个PVCR的表达和/或敏感性的量添加到淡水中，PVCR调节剂是将鱼的嗅觉感测改变为 气味; 向淡水添加饲料，含有气味剂的饲料和一定量的NaCl足以有助于在饲料消耗时鱼血清中PVCR调节剂的水平显着提高，由此将鱼加上加味剂 ; 将印迹鱼转移到海水中; 并在所述海水中提供所述加臭剂的来源，所述海水中转印有所述印迹鱼，从而使所述印迹鱼与所述加臭剂反应。 本发明还包括归巢或吸引鱼的方法，以及通过调节鱼的嗅觉装置中PVCR的表达和/或敏感性来排斥鱼的方法。
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公开(公告)号：US06481379B1
公开(公告)日：2002-11-19
申请号：US09687372
申请日：2000-10-12
IPC分类号： A01K6100
摘要： The invention relates to methods, compositions and kits for improving the raising of pre-adult anadromous fish, or preparing pre-adult anadromous fish for transfer to seawater. The methods involve adding PVCR modulators (e.g., calcium and/or magnesium) to the freshwater in an amount sufficient to increase expression and/or sensitivity of at least one PVCR; and adding feed for fish consumption to the freshwater, wherein the feed contains an amount of NaCl sufficient to contribute to a significantly increased level of the PVCR modulator in serum of the pre-adult anadromous fish.
摘要翻译： 本发明涉及用于改善成年前成鱼的饲养的方法，组合物和试剂盒，或者用于制备用于转运至海水的成年前成鱼。 所述方法包括以足以增加至少一种PVCR的表达和/或敏感性的量向淡水中加入PVCR调节剂（例如钙和/或镁） 并向淡水添加鱼类消费的饲料，其中饲料含有足以有助于在成年前成鱼的血清中PVCR调节剂水平显着提高的量的NaCl。
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