公开(公告)号：US06881723B1

公开(公告)日：2005-04-19

申请号：US09434830

申请日：1999-11-05

Applicant: Deborah L. Fuller ,  James T. Fuller

Inventor： Deborah L. Fuller ,  James T. Fuller

IPC: A61K39/385 ,  A61K48/00 ,  C07H21/04 ,  C12N15/74

CPC classification number: A61K39/385 ,  A61K2039/53 ,  A61K2039/57 ,  A61K2039/6075

Abstract: Recombinant nucleic acid molecules are described. The molecules have a first sequence encoding a Hepatitis B virus core antigen and a second sequence encoding at least one T cell epitope inserted within the first sequence. Vectors and compositions containing these molecules are also described. Methods of eliciting an immune response using these molecules are also described.

Abstract translation: 描述重组核酸分子。 分子具有编码乙型肝炎病毒核心抗原的第一序列和编码插入第一序列内的至少一个T细胞表位的第二序列。 还描述了含有这些分子的载体和组合物。 还描述了使用这些分子引发免疫应答的方法。

公开(公告)号：US6140075A

公开(公告)日：2000-10-31

申请号：US902486

申请日：1997-07-29

Applicant: David R. Russell ,  James T. Fuller ,  Michael J. Miller

Inventor： David R. Russell ,  James T. Fuller ,  Michael J. Miller

IPC: C07K14/415 ,  C07K16/28 ,  C07K16/30 ,  C12N15/29 ,  C12N15/82 ,  C12N15/00 ,  C12N15/81

CPC classification number: C07K14/415 ,  C07K16/2866 ,  C07K16/30 ,  C12N15/8221 ,  C12N15/8257 ,  C12N15/8258 ,  C07K2317/13 ,  C07K2317/622

Abstract: A method for producing antibodies and plant protein toxins in plant cells is described which includes the steps of providing a genetic construct to encode the secretable protein, delivering copies of the construct into a liquid suspension culture of tobacco cells, selecting for cells that have acquired the genetic construct, and allowing the desired protein to accumulate in the liquid media in which the plant cells are grown, and isolating the desired protein away from the tobacco cells.

Abstract translation: 描述了在植物细胞中产生抗体和植物蛋白毒素的方法，其包括以下步骤：提供编码可分泌蛋白质的遗传构建体，将构建体的拷贝递送到烟草细胞的液体悬浮培养物中，选择获得 遗传构建体，并使所需的蛋白质积聚在植物细胞生长的液体培养基中，并将期望的蛋白质从烟草细胞中分离出来。

公开(公告)号：US07699801B2

公开(公告)日：2010-04-20

申请号：US11752173

申请日：2007-05-22

Applicant: Joel R. Haynes ,  Georg Widera ,  James T. Fuller ,  Timothy Shipley ,  Deborah Fuller ,  Mary Wu

Inventor： Joel R. Haynes ,  Georg Widera ,  James T. Fuller ,  Timothy Shipley ,  Deborah Fuller ,  Mary Wu

IPC: A61M31/00 ,  C12N15/85 ,  C12N15/87

CPC classification number: A61K33/24 ,  A61K39/0011 ,  A61K39/015 ,  A61K39/12 ,  A61K39/145 ,  A61K39/21 ,  A61K39/292 ,  A61K45/06 ,  A61K2039/53 ,  A61K2039/545 ,  A61K2039/55572 ,  A61K2039/55577 ,  A61K2039/57 ,  C07K14/005 ,  C12N15/895 ,  C12N2730/10122 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2760/16122 ,  C12N2760/16134 ,  Y02A50/412

Abstract: Reagents useful in nucleic acid immunization techniques are described. More particularly, adjuvanted genetic vaccine compositions are described, as are methods of using those compositions for inducing an enhanced immune response against a selected antigen.

Abstract translation: 描述了可用于核酸免疫技术的试剂。 更具体地，描述了佐剂化的遗传疫苗组合物，以及使用这些组合物诱导针对所选抗原的增强的免疫应答的方法。

公开(公告)号：US6080560A

公开(公告)日：2000-06-27

申请号：US279772

申请日：1994-07-25

Applicant: David R. Russell ,  James T. Fuller

Inventor： David R. Russell ,  James T. Fuller

IPC: C07K14/415 ,  C07K16/28 ,  C07K16/30 ,  C12N15/29 ,  C12N15/82 ,  A01H4/00 ,  C12N15/00

CPC classification number: C07K16/30 ,  C07K14/415 ,  C07K16/2866 ,  C12N15/8221 ,  C12N15/8257 ,  C12N15/8258 ,  C07K2317/13 ,  C07K2317/622

Abstract: A method for producing antibodies in plant cells including the steps of providing a genetic construct that encodes a secretable mammalian single chain antibody, delivering copies of the construct into a liquid suspension culture of tobacco cells, selecting for cells that have acquired the genetic construct, allowing the antibody to accumulate in the liquid to a concentration over 25 mg/l and isolating the antibody away from the tobacco cells.

Abstract translation: 一种用于在植物细胞中产生抗体的方法，包括以下步骤：提供编码可分泌哺乳动物单链抗体的遗传构建体，将构建体的拷贝递送到烟草细胞的液体悬浮培养物中，选择获得遗传构建体的细胞，允许 该抗体在液体中积累至浓度超过25mg / l，并​​将抗体与烟草细胞分离。

公开(公告)号：US07223739B1

公开(公告)日：2007-05-29

申请号：US09433777

申请日：1999-11-03

Applicant: Joel R. Haynes ,  Georg Widera ,  James T. Fuller ,  Timothy Shipley ,  Deborah Fuller ,  Mary Wu

Inventor： Joel R. Haynes ,  Georg Widera ,  James T. Fuller ,  Timothy Shipley ,  Deborah Fuller ,  Mary Wu

IPC: A61K48/00 ,  A61K47/02 ,  A61K45/00 ,  C12N15/63

CPC classification number: A61K33/24 ,  A61K39/0011 ,  A61K39/015 ,  A61K39/12 ,  A61K39/145 ,  A61K39/21 ,  A61K39/292 ,  A61K45/06 ,  A61K2039/53 ,  A61K2039/545 ,  A61K2039/55572 ,  A61K2039/55577 ,  A61K2039/57 ,  C07K14/005 ,  C12N15/895 ,  C12N2730/10122 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2760/16122 ,  C12N2760/16134 ,  Y02A50/412

Abstract: Reagents useful in nucleic acid immunization techniques are described. More particularly, adjuvanted genetic vaccine compositions are described, as are methods of using those compositions for inducing an enhanced immune response against a selected antigen.

Abstract translation: 描述了可用于核酸免疫技术的试剂。 更具体地，描述了佐剂化的遗传疫苗组合物，以及使用这些组合物诱导针对所选抗原的增强的免疫应答的方法。

公开(公告)号：US20090017527A1

公开(公告)日：2009-01-15

申请号：US11752173

申请日：2007-05-22

Applicant: Joel R. HAYNES ,  Georg Widera ,  James T. Fuller ,  Timothy Shipley ,  Deborah Fuller ,  Mary Wu

Inventor： Joel R. HAYNES ,  Georg Widera ,  James T. Fuller ,  Timothy Shipley ,  Deborah Fuller ,  Mary Wu

IPC: C12M1/00

CPC classification number: A61K33/24 ,  A61K39/0011 ,  A61K39/015 ,  A61K39/12 ,  A61K39/145 ,  A61K39/21 ,  A61K39/292 ,  A61K45/06 ,  A61K2039/53 ,  A61K2039/545 ,  A61K2039/55572 ,  A61K2039/55577 ,  A61K2039/57 ,  C07K14/005 ,  C12N15/895 ,  C12N2730/10122 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2760/16122 ,  C12N2760/16134 ,  Y02A50/412

Abstract: Reagents useful in nucleic acid immunization techniques are described. More particularly, adjuvanted genetic vaccine compositions are described, as are methods of using those compositions for inducing an enhanced immune response against a selected antigen.

Abstract translation: 描述了可用于核酸免疫技术的试剂。 更具体地，描述了佐剂化的遗传疫苗组合物，以及使用这些组合物诱导针对所选抗原的增强的免疫应答的方法。

公开(公告)号：US4623206A

公开(公告)日：1986-11-18

申请号：US687528

申请日：1984-12-28

Applicant: James T. Fuller

Inventor： James T. Fuller

IPC: G06F1/20 ,  H01M2/10 ,  H01R12/55 ,  H05K3/30 ,  H05K3/34 ,  H05K5/00 ,  H01R9/09 ,  A47B97/00

CPC classification number: H05K3/301 ,  H01M2/1044 ,  H05K5/0086 ,  H01R12/58 ,  H05K2201/10295 ,  H05K2201/10393 ,  H05K2201/10643 ,  H05K3/3447

Abstract: There is disclosed herein a spring battery retainer that is used to secure a small "coin" or "button" battery to a printed circuit (PC) card or the like. Use of the spring battery retainer provides for positive location of the battery, inexpensive electrical contacts for the battery terminals and positive spring compression that is necessary to insure hard contact with the cathode and the anode terminals of the battery. The battery is easily installed and removed by slightly lifting one end of the battery retainer and sliding the battery in or out. A bend in the spring battery retainer provides sufficient hold-down pressure so that the battery makes reliable contact with an electrical pad located on a PC board. The spring battery retainer is inserted into the PC card and then soldered to it in much the same way as a standard electrical component such as a resistor. The dimensions of the spring battery retainer can be varied so that coin or button batteries of various sizes can be securely fastened to a PC card.

Abstract translation: 这里公开了用于将小的“硬币”或“按钮”电池固定到印刷电路（PC）卡等的弹簧电池保持器。 使用弹簧电池固定器可以提供电池的正确位置，为电池端子提供便宜的电气触点和正确的弹簧压缩，以确保与电池的阴极和阳极端子的硬接触。 通过轻轻抬起电池固定器的一端并将电池滑入或滑出即可轻松安装和拆卸电池。 弹簧电池固定器中的弯曲部提供足够的压紧压力，使得电池与位于PC板上的电焊垫可靠接触。 将弹簧电池固定器插入PC卡，然后以与电阻器等标准电气部件相同的方式焊接到PC卡。 可以改变弹簧电池固定器的尺寸，使得各种尺寸的硬币或纽扣电池可以牢固地固定在PC卡上。