公开(公告)号：US4357363A

公开(公告)日：1982-11-02

申请号：US323623

申请日：1981-11-20

申请人： Zona R. Pierce ,  David S. Frank

发明人： Zona R. Pierce ,  David S. Frank

IPC分类号： G01N31/22 ,  G01N33/52 ,  G01N33/543 ,  G01N33/549

CPC分类号： G01N33/549 ,  G01N31/22 ,  G01N31/221 ,  G01N33/525 ,  G01N33/54386 ,  Y10T428/24826

摘要： An element for the analysis or transport of liquid, especially aqueous liquids, contains a structure comprising a plurality of heat-stable, organo-polymeric particles non-swellable in and impermeable to the liquid, and an adhesive concentrated at particle surface areas contiguous to adjacent particles bonding the particles into a coherent, three-dimensional lattice that is non-swellable in the liquid. A substantial portion of the particle surface area in this lattice structure is therefore effectively free from adhesive. The lattice structure has interconnected void spaces among the particles representing a total void volume of about 25 to 80 percent to provide for transport of the liquid. The adhesive comprises an organic polymer different from that of the particles and insoluble in the liquid under analysis. The amount of adhesive in the structure is less than 10 weight percent of the particles.The particulate structure of these elements can contain interactive compositions useful for the analysis of various substances in liquids, especially high molecular weight proteinaceous substances in aqueous biological liquids. Multi-zone elements containing, in fluid contact, at least two zones having a particulate structure as described above or one such zone together with other functional zones are also disclosed.

摘要翻译： 用于分析或运输液体，特别是含水液体的元件包含一种结构，其包含多个不溶于液体的不可溶胀且不可渗透的热稳定的有机聚合物颗粒，以及浓缩在邻近相邻的颗粒表面区域的粘合剂 将颗粒结合成在液体中不溶胀的相干的三维晶格。 因此，该晶格结构中的大部分颗粒表面积实际上没有粘合剂。 晶格结构在颗粒之间具有互连的空隙，表示总空隙体积为约25至80％以提供液体的输送。 粘合剂包含不同于颗粒的有机聚合物，并且不溶于待分析的液体中。 结构中的粘合剂的量小于颗粒的10重量％。 这些元素的颗粒结构可以包含可用于分析液体中的各种物质，特别是含水生物液体中的高分子量蛋白质物质的相互作用的组合物。 还公开了包含流体接触的至少两个具有上述颗粒结构的区域或一个这样的区域与其它功能区域的多区域元件。

公开(公告)号：US4258001A

公开(公告)日：1981-03-24

申请号：US973669

申请日：1978-12-27

申请人： Zona R. Pierce ,  David S. Frank

发明人： Zona R. Pierce ,  David S. Frank

IPC分类号： G01N33/48 ,  G01N1/00 ,  G01N31/22 ,  G01N33/52 ,  G01N33/543 ,  G01N33/549 ,  G01N33/54 ,  G01N33/68

CPC分类号： G01N31/22 ,  G01N33/525 ,  G01N33/54386 ,  G01N33/549 ,  Y10S435/805 ,  Y10T428/254 ,  Y10T428/269 ,  Y10T428/2998

摘要： An element for the analysis or transport of liquid, especially aqueous liquids, contains a structure comprising a plurality of heat-stable, organo-polymeric particles non-swellable in and impermeable to the liquid, and an adhesive concentrated at particle surface areas contiguous to adjacent particles bonding the particles into a coherent, three-dimensional lattice that is non-swellable in the liquid. A substantial portion of the particle surface area in this lattice structure is therefore effectively free from adhesive. The lattice structure has interconnected void spaces among the particles representing a total void volume of about 25 to 80 percent to provide for transport of the liquid. The adhesive comprises an organic polymer different from that of the particles and insoluble in the liquid under analysis. The amount of adhesive in the structure is less than 10 weight percent of the particles.The particulate structure of these elements can contain interactive compositions useful for the analysis of various substances in liquids, especially high molecular weight proteinaceous substances in aqueous biological liquids. Multi-zone elements containing, in fluid contact, at least two zones having a particulate structure as described above or one such zone together with other functional zones are also disclosed.

摘要翻译： 用于分析或运输液体，特别是含水液体的元件包含一种结构，其包含多个不溶于液体的不可溶胀且不可渗透的热稳定的有机聚合物颗粒，以及浓缩在邻近相邻的颗粒表面区域的粘合剂 将颗粒结合成在液体中不溶胀的相干的三维晶格。 因此，该晶格结构中的大部分颗粒表面积实际上没有粘合剂。 晶格结构在颗粒之间具有互连的空隙，表示总空隙体积为约25至80％以提供液体的输送。 粘合剂包含不同于颗粒的有机聚合物，并且不溶于待分析的液体中。 结构中的粘合剂的量小于颗粒的10重量％。 这些元素的颗粒结构可以包含可用于分析液体中的各种物质，特别是含水生物液体中的高分子量蛋白质物质的相互作用的组合物。 还公开了包含流体接触的至少两个具有上述颗粒结构的区域或一个这样的区域与其它功能区域的多区域元件。

公开(公告)号：US4381921A

公开(公告)日：1983-05-03

申请号：US193508

申请日：1980-10-02

申请人： Zona R. Pierce ,  David S. Frank

发明人： Zona R. Pierce ,  David S. Frank

IPC分类号： G01N31/22 ,  G01N33/28 ,  G01N33/52 ,  G01N33/543 ,  G01N33/544 ,  G01N33/54 ,  G01N33/68

CPC分类号： G01N33/544 ,  G01N31/22 ,  G01N31/221 ,  G01N33/525 ,  G01N33/54386 ,  Y10S435/966

摘要： An element for the analysis or transport of liquid, especially aqueous liquids, contains a structure comprising a plurality of heat-stable, organo-polymeric particles non-swellable in and impermeable to the liquid, and an adhesive concentrated at particle surface areas contiguous to adjacent particles bonding the particles into a coherent, three-dimensional lattice that is non-swellable in the liquid. A substantial portion of the particle surface area in this lattice structure is therefore effectively free from adhesive. The lattice structure has interconnected void spaces among the particles representing a total void volume of about 25 to 80 percent to provide for transport of the liquid. The adhesive comprises an organic polymer different from that of the particles and insoluble in the liquid under analysis. The amount of adhesive in the structure is less than 10 weight percent of the particles.The particulate structure of these elements can contain interactive compositions useful for the analysis of various substances in liquids, especially high molecular weight proteinaceous substances in aqueous biological liquids. Multi-zone elements containing, in fluid contact, at least two zones having a particulate structure as described above or one such zone together with other functional zones are also disclosed.

公开(公告)号：US4269938A

公开(公告)日：1981-05-26

申请号：US18532

申请日：1979-03-08

申请人： David S. Frank

发明人： David S. Frank

IPC分类号： G01N33/573 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/28 ,  G01N33/535 ,  G01N31/14 ,  C09K3/00 ,  G01N31/22

CPC分类号： G01N33/535 ,  C12Q1/28 ,  C12Q2326/40 ,  Y10S435/97 ,  Y10T436/144444 ,  Y10T436/148888

摘要： A new peroxidase assay for substances demonstrating peroxidative activity is described which is based on the peroxidase catalyzed oxidation of dichlorofluorescin to dichlorofluorescein by peroxide. The dichlorofluorescin is formed from diacetyldichlorofluorescin either by the direct addition of peroxide or by the inclusion of a peroxide generating system (e.g., glucose plus glucose oxidase or uric acid and uricase) in the reaction mixture. As long as all reactants other than the peroxidase are present in excess, the rate of fluorescence increase is linearly related to the amount of peroxidase in the sample under assay.

摘要翻译： 描述了一种用于证明过氧化活性的物质的新型过氧化物酶测定法，其基于过氧化物酶催化的过氧化物将二氯荧光素氧化成二氯荧光素。 二氯荧光素由二乙酰二氯荧光素通过直接加入过氧化物或通过在反应混合物中包含过氧化物产生系统（例如葡萄糖加葡萄糖氧化酶或尿酸和尿酸酶）形成。 只要过氧化物酶以外的所有反应物都过量存在，荧光增加速率与试验中样品中过氧化物酶的量线性相关。

公开(公告)号：US4259313A

公开(公告)日：1981-03-31

申请号：US66962

申请日：1979-08-16

申请人： David S. Frank ,  Michael W. Sundberg

发明人： David S. Frank ,  Michael W. Sundberg

IPC分类号： G01N33/533 ,  G01N33/58 ,  G01N31/00 ,  G01N31/22 ,  G01N33/00 ,  G01N33/48

CPC分类号： G01N33/585 ,  G01N33/533 ,  Y10S436/80

摘要： There are described stabilized fluorescent labels comprising rare earth chelate incorporated in polymeric beads derived from a latex. Labeled reactive proteinaceous species such as labeled immunoreagents comprising the stabilized fluorescent labels having protein absorbed or covalently bonded thereto are also described.

摘要翻译： 描述了稳定的荧光标记，其包含掺入衍生自胶乳的聚合物珠中的稀土螯合物。 还描述了标记的反应性蛋白质物质，例如标记的免疫反应物，其包含具有吸收或共价键合的蛋白质的稳定的荧光标记物。

公开(公告)号：US4194063A

公开(公告)日：1980-03-18

申请号：US880827

申请日：1978-02-24

申请人： David S. Frank ,  Ignazio S. Ponticello

发明人： David S. Frank ,  Ignazio S. Ponticello

IPC分类号： C12Q1/58 ,  G01N33/14

CPC分类号： C12Q1/58

摘要： Methods, compositions and elements for detecting a nitrogen-containing compound capable of releasing ammonia upon enzymatic action are described. The method comprises contacting in an aqueous medium a sample suspected of containing such a compound and a novel assay composition comprising at least one enzyme which catalyzes the decomposition of the compound to ammonia and detecting the ammonia by condensation with a .beta.-diketone in the presence of an aldehyde, preferably formaldehyde, and measuring the color or fluorescence produced by any resulting dihydropyridine. According to a preferred embodiment, the aldehyde is generated in situ and the .beta.-diketone is polymeric.The foregoing assay composition can be incorporated into single-layer or multilayer analytical elements of the type known in the prior art. A preferred such element comprises an isotropically porous spreading layer in fluid contact with a reagent layer. The spreading layer is most preferably non-fibrous.

摘要翻译： 描述了用于检测酶促作用时能够释放氨的含氮化合物的方法，组合物和元件。 所述方法包括在水性介质中接触怀疑含有这种化合物的样品和新的测定组合物，所述新型测定组合物包含至少一种催化该化合物分解成氨的酶，并在β-二酮存在下与β-二酮缩合来检测氨 醛，优选甲醛，并测量任何所得二氢吡啶产生的颜色或荧光。 根据优选的实施方案，醛原位生成，β-二酮是聚合物。 上述测定组合物可以结合到现有技术中已知的类型的单层或多层分析元件中。 优选的这种元件包括与试剂层流体接触的各向同性的多孔扩散层。 铺展层最优选非纤维状。

公开(公告)号：US4555396A

公开(公告)日：1985-11-26

申请号：US452260

申请日：1982-12-22

申请人： David S. Frank ,  Robert T. Belly

发明人： David S. Frank ,  Robert T. Belly

IPC分类号： G01N1/30 ,  C09B57/00 ,  G01N33/48 ,  G01N33/50 ,  C12Q1/04

CPC分类号： G01N33/5005

摘要： Disclosed herein is an improvement in a method for distinguishing cells in a biological sample by staining with a dye, wherein the improvement comprises employing as the dye a compound of the formula ##STR1## wherein G is O or S;R.sup.1, R.sup.3, and R.sup.5 are independently selected from the group consisting of hydrogen, alkyl, aryl, aralkyl, amino, styryl, bis(diaryl)vinylene, and ##STR2## wherein R is hydrogen or alkyl;Z represents the elements necessary to complete a basic heterocyclic ring system of the type used in cyanine dyes;n is 0 or 1;R.sup.2 is hydrogen or, taken together with either R.sup.1 or R.sup.3, represents the elements needed to complete an aromatic or a carbocyclic ring system;R.sup.4 is hydrogen or, taken together with either R.sup.3 or R.sup.5, represents the elements needed to complete an aromatic or a carbocyclic ring system; andX.sup.- is an anion.

摘要翻译： 本文公开了通过用染料染色来区分生物样品中的细胞的方法的改进，其中所述改进包括使用式为“IMAGE”的化合物，其中G为O或S; R 1，R 3和R 5独立地选自氢，烷基，芳基，芳烷基，氨基，苯乙烯基，双（二芳基）亚乙烯基，和其中R是氢或烷基; Z表示完成花青染料中使用的类型的碱性杂环系统所需的元素; n为0或1; R2是氢或与R1或R3一起代表完成芳族或碳环系所需的元素; R4是氢或与R3或R5一起代表完成芳族或碳环系所需的元素; 而X-是阴离子。

公开(公告)号：US4283382A

公开(公告)日：1981-08-11

申请号：US952424

申请日：1978-10-18

申请人： David S. Frank ,  Michael W. Sundberg

发明人： David S. Frank ,  Michael W. Sundberg

IPC分类号： G01N33/533 ,  G01N33/58 ,  G01N31/00 ,  G01N31/22 ,  G01N33/00 ,  G01N33/48

CPC分类号： G01N33/585 ,  G01N33/533 ,  Y10S436/80 ,  Y10S436/827

摘要： There are described stabilized fluorescent labels comprising rare earth chelate incorporated in polymeric beads derived from a latex. Labeled reactive proteinaceous species such as labeled immunoreagents comprising the stabilized fluorescent labels having portein adsorbed or covalently bonded thereto are also described.

摘要翻译： 描述了稳定的荧光标记，其包含掺入衍生自胶乳的聚合物珠中的稀土螯合物。 还描述了标记的反应性蛋白质物质，例如标记的免疫反应物，其包含具有吸附或共价键合的portein的稳定的荧光标记物。

公开(公告)号：US4840784A

公开(公告)日：1989-06-20

申请号：US764151

申请日：1985-08-09

申请人： David S. Frank ,  Robert T. Belly

发明人： David S. Frank ,  Robert T. Belly

IPC分类号： G01N33/50 ,  G01N33/52

CPC分类号： G01N33/5005 ,  G01N33/52

摘要： Disclosed herein is an improvement in a method for distinguishing cells in a biological sample by staining with a dye, wherein the improvement comprises employing as the dye a compound of the formula ##STR1## wherein G is O or S;R.sup.1, R.sup.3, and R.sup.5 are independently selected from the group consisting of hydrogen, alkyl, aryl, aralkyl, amino, styryl, bis(diaryl)vinylene, and ##STR2## wherein R is hydrogen or alkyl;Z represents the elements necessary to complete a basic heterocyclic ring system of the type used in cyanine dyes;n is 0 or 1;R.sup.2 is hydrogen or, taken together with either R.sup.1 or R.sup.3, represents the elements needed to complete an aromatic or a carbocyclic ring system;R.sup.4 is hydrogen or, taken together with either R.sup.3 or R.sup.5, represents the elements needed to complete an aromatic or a carbocyclic ring system; andX.sup.- is an anion.

摘要翻译： 本文公开了通过用染料染色来区分生物样品中细胞的方法的改进，其中改进包括使用式为“IMAGE”的化合物，其中G为O或S; R 1，R 3和R 5独立地选自氢，烷基，芳基，芳烷基，氨基，苯乙烯基，双（二芳基）亚乙烯基，和其中R是氢或烷基; Z表示完成花青染料中使用的类型的碱性杂环系统所需的元素; n为0或1; R2是氢或与R1或R3一起代表完成芳族或碳环系所需的元素; R4是氢或与R3或R5一起代表完成芳族或碳环系所需的元素; 而X-是阴离子。