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9 条记录 (耗时 0.016 秒)

    REGULATORY ELEMENTS FOR DELIVERY TO THE LIVER
    1.
    发明申请
    REGULATORY ELEMENTS FOR DELIVERY TO THE LIVER 审中-公开
    向肝脏递送的法规要素

    公开(公告)号：US20080070297A1

    公开(公告)日：2008-03-20

    申请号：US11938906

    申请日：2007-11-13

    申请人： David SOUZA ,  Donna ARMENTANO ,  Samuel WADSWORTH

    发明人： David SOUZA ,  Donna ARMENTANO ,  Samuel WADSWORTH

    IPC分类号： C12N15/861 ,  C12N15/11

    CPC分类号： C12N15/86 ,  A61K48/00 ,  A61K48/0058 ,  C12N15/85 ,  C12N2710/10343 ,  C12N2799/022 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2830/85

    摘要： The invention is directed to novel combinations of liver specific enhancers and promoter elements for achieving persistent transgene expression in the liver. The liver specific enhancer elements may be derived from either the human serum albumin, prothrombin, α-1microglobulin or aldolase genes in single copies or in multimerized from linked to elements derived from the cytomegalovirus intermediate early (CMV), α-1-antitrypsin or albumin promoters. In a preferred embodiment of the invention, an adenoviral vector comprising a liver specific enhancer/promoter combination operably linked to a transgene is administered to recipient cells. In other embodiments of the invention, adeno-associated viral vectors, retroviral vectors, lentiviral vectors or a plasmid comprising the liver specific enhancer/promoter combination linked to a transgene is administered to recipient cells. Also within the scope of the invention are promoter elements derived from the human prothrombin gene and the β-fibrinogen gene.

    摘要翻译： 本发明涉及用于实现肝脏中持续转基因表达的肝特异性增强子和启动子元件的新型组合。 肝脏特异性增强子元件可以衍生自单拷贝中的人血清白蛋白，凝血酶原，α-1微球蛋白或醛缩酶基因，或与源自巨细胞病毒中间体早期（CMV），α-1-抗胰蛋白酶或白蛋白的元件连接的多聚化 发起人。 在本发明的优选实施方案中，向受体细胞施用包含可操作地连接到转基因的肝特异性增强子/启动子组合的腺病毒载体。 在本发明的其它实施方案中，将腺相关病毒载体，逆转录病毒载体，慢病毒载体或包含与转基因连接的肝特异性增强子/启动子组合的质粒施用于受体细胞。 也在本发明范围内的是源自人凝血酶原基因和β-纤维蛋白原基因的启动子元件。

    ERROR PATTERN COMPENSATION
    2.
    发明申请
    ERROR PATTERN COMPENSATION 审中-公开
    错误图案补偿

    公开(公告)号：US20160236418A1

    公开(公告)日：2016-08-18

    申请号：US14841603

    申请日：2015-08-31

    申请人： Michael Daniel Armani ,  David Souza Jones

    发明人： Michael Daniel Armani ,  David Souza Jones

    IPC分类号： B29C67/00 ,  G05B19/402

    CPC分类号： G05B19/402 ,  B29C48/30 ,  B29C48/92 ,  B29C64/106 ,  B29K2105/0058 ,  B29K2879/00 ,  B29K2883/00 ,  B29K2885/00 ,  B29K2909/02 ,  B29K2909/08 ,  B29K2995/0037 ,  B33Y30/00

    摘要： An accelerometer is supported on a print head assembly to determine actual acceleration, and a computer system is used to obtain and store information about error in movement along a specified axis. The method used is to activate a motor for a selected axis at a particular location, then calculate an expected acceleration. The next step is to measure the actual acceleration data and then compute error data. Following that is a step to store error data in the memory as a function of position and error. These steps are repeated for N locations along each specified axis. Then, this error data is referred to during actual printing operations to reduce error.

    摘要翻译： 在打印头组件上支撑加速度计以确定实际的加速度，并且使用计算机系统来获取和存储关于沿着指定轴的运动中的误差的信息。 所使用的方法是在特定位置激活所选轴的电动机，然后计算预期的加速度。 下一步是测量实际的加速度数据，然后计算出错误数据。 以下是将存储器中的错误数据作为位置和错误的函数进行存储的步骤。 沿着每个指定轴的N个位置重复这些步骤。 然后，在实际打印操作期间参考该错误数据以减少错误。

    3D PRINTER
    3.
    发明申请
    3D PRINTER 审中-公开
    3D打印机

    公开(公告)号：US20160236407A1

    公开(公告)日：2016-08-18

    申请号：US14840314

    申请日：2015-08-31

    申请人： Michael Daniel Armani ,  David Souza Jones

    发明人： Michael Daniel Armani ,  David Souza Jones

    IPC分类号： B29C67/00 ,  B29C47/12

    CPC分类号： G05B19/402 ,  B29C48/30 ,  B29C48/92 ,  B29C64/106 ,  B29K2105/0058 ,  B29K2879/00 ,  B29K2883/00 ,  B29K2885/00 ,  B29K2909/02 ,  B29K2909/08 ,  B29K2995/0037 ,  B33Y30/00

    摘要： A 3D printer has a casing, a nozzle for printing, an extruder, a heating element, and a print bed. The casing encloses a region above and around the print bed to form a printing zone. The apparatus has a plurality of micro motors for controlling movement of the nozzle during printing. Further, the 3D printer has safer components as compared with the prior art, ideal for use in home and education situations, and includes a rounded edge design of the printer, an improved small and enclosed area of the printer heating element, and a lower power system, reducing the risk of electrical shock.

    摘要翻译： 3D打印机具有壳体，用于打印的喷嘴，挤出机，加热元件和打印床。 套管围绕打印台上方和周围区域形成打印区域。 该装置具有多个用于在打印期间控制喷嘴的运动的微型马达。 此外，与现有技术相比，3D打印机具有比现有技术更安全的组件，其适用于家庭和教育环境，并且包括打印机的圆角边缘设计，打印机加热元件的改进的小且封闭区域以及较低功率 系统，降低电击的风险。

    Muscle-specific expression vectors
    4.
    发明申请
    Muscle-specific expression vectors 审中-公开
    肌肉特异性表达载体

    公开(公告)号：US20110212529A1

    公开(公告)日：2011-09-01

    申请号：US11716899

    申请日：2007-03-12

    申请人： David Souza ,  Donna Armentano

    发明人： David Souza ,  Donna Armentano

    IPC分类号： C12N15/85 ,  C07H21/04 ,  C12N1/00 ,  C12N5/10

    CPC分类号： C12N15/85 ,  C12N2830/008 ,  C12N2830/85

    摘要： The invention is directed to novel combinations of muscle-specific enhancers and promoter elements useful for achieving persistent expression in the muscle or myocyctes. The muscle-specific promoter elements are derived from a muscle creatine kinase promoter, a troponin I promoter, a skeletal alpha-actin promoter, or a desmin promoter. The muscle-specific enhancer elements are derived from either troponin I internal regulatory elements, muscle creatine kinase enhancers, or desmin enhancers.

    摘要翻译： 本发明涉及用于在肌肉或肌细胞中实现持续表达的肌肉特异性增强子和启动子元件的新颖组合。 肌肉特异性启动子元件来源于肌肉肌酸激酶启动子，肌钙蛋白I启动子，骨骼α-肌动蛋白启动子或结蛋白启动子。 肌肉特异性增强子元件来自肌钙蛋白I内调节元件，肌肉肌酸激酶增强子或结蛋白增强子。

    3D PRINTER
    6.
    发明申请
    3D PRINTER 审中-公开
    3D打印机

    公开(公告)号：US20160236409A1

    公开(公告)日：2016-08-18

    申请号：US14840361

    申请日：2015-08-31

    申请人： Michael Daniel Armani ,  David Souza Jones

    发明人： Michael Daniel Armani ,  David Souza Jones

    IPC分类号： B29C67/00 ,  B29C47/12

    CPC分类号： G05B19/402 ,  B29C48/30 ,  B29C48/92 ,  B29C64/106 ,  B29K2105/0058 ,  B29K2879/00 ,  B29K2883/00 ,  B29K2885/00 ,  B29K2909/02 ,  B29K2909/08 ,  B29K2995/0037 ,  B33Y30/00

    摘要： A 3D printer has a casing, a nozzle for printing, an extruder, a heating element, and a print bed. The casing encloses a region above and around the print bed to form a printing zone. Further, the 3D printer has features adapted for low-noise operation, and is already suitably quiet enough for use in a low-noise environment because it does not generate loud sounds. Specifically, in a quiet office with 37-38 dB of noise, noise emissions were measured while the present invention constructed a 3D printed model, and at six inches from the extruder on the 3D printer, the noise emissions measured 39-58 dB, at three feet away measured 38-43 dB, and at six feet away measured around 37-40 dB.

    摘要翻译： 3D打印机具有壳体，用于打印的喷嘴，挤出机，加热元件和打印床。 套管围绕打印台上方和周围区域形成打印区域。 此外，3D打印机具有适于低噪声操作的特征，并且已经适当地安静到足以在低噪声环境中使用，因为它不会产生大声的声音。 具体来说，在具有37-38dB噪声的安静办公室中，测量噪声发射，而本发明构造了3D印刷模型，并且在距离3D打印机上的挤出机6英寸处，噪声发射测量为39-58dB，在 三英尺远，测得38-43分贝，六英尺处测量约37-40分贝。