公开(公告)号：US20070127840A1

公开(公告)日：2007-06-07

申请号：US11295959

申请日：2005-12-07

申请人： William Gardner ,  Du-Yong Ng

发明人： William Gardner ,  Du-Yong Ng

IPC分类号： G06K9/40 ,  H04N1/409

CPC分类号： G06K9/40 ,  H04N1/4097

摘要： A method for removing streaks from a scanned image includes performing a scanner light source OFF scan to generate a dark image having M rows of pixels and N+W columns of pixels; generating a line noise profile for the N columns of pixels of the dark image; performing a scanner light source ON scan to generate a regular image having M rows of pixels and N+W columns of pixels; processing the W columns of pixels of the regular image to detect a magnitude of the streak in the regular image; use the magnitude of the streak and the line noise profile to predict an amount of dark noise removal for each of the N columns of pixels of the regular image at the location of the streak; and subtracting the predicted amount of dark noise removal for each of the N columns of pixels from the respective N columns of pixels of the regular image.

摘要翻译： 用于从扫描图像中去除条纹的方法包括执行扫描仪光源OFF扫描以产生具有M行像素和N + W列像素的暗图像; 生成暗图像的N列像素的线噪声分布; 执行扫描仪光源ON扫描以产生具有M行像素和N + W列像素的规则图像; 处理常规图像的W列的像素以检测正常图像中的条纹的大小; 使用条纹和线噪声分布的大小来预测在条纹位置处的常规图像的N列像素中的每一个的暗噪声去除量; 并从所述常规图像的各N列像素中减去所述N列像素中的每一个的暗噪声去除的预测量。

公开(公告)号：US20100098716A1

公开(公告)日：2010-04-22

申请号：US12555743

申请日：2009-09-08

申请人： Haitao WANG ,  Chungsheng Mao ,  Jizhi Li ,  Jing XU ,  Rui ZHANG ,  Ling Wang ,  DU Yong ,  Longbin LIU

发明人： Haitao WANG ,  Chungsheng Mao ,  Jizhi Li ,  Jing XU ,  Rui ZHANG ,  Ling Wang ,  DU Yong ,  Longbin LIU

IPC分类号： A61K39/395 ,  A61K38/00

CPC分类号： C07K14/505 ,  A61K9/0019 ,  A61K38/00 ,  A61K47/6811 ,  C07K2317/53 ,  C07K2319/30

摘要： A recombinant fusion protein comprising a human erythropoietin peptide portion linked to an immunoglobulin peptide portion is described. The fusion protein has a prolonged half-life in vivo in comparison to naturally occurring or recombinant native human erythropoietin. In one embodiment of the invention, the protein has a half-life in vivo at least three fold higher than native human erythropoietin. The fusion protein also exhibits enhanced erythropoietic bioactivity in comparison to native human erythropoietin. In one embodiment, the fusion protein comprises the complete peptide sequence of a human erythropoietin (EPO) molecule and the peptide sequence of an Fc fragment of human immunoglobulin IgG1. The Fc fragment in the fusion protein includes the hinge region, CH2 and CH3 domains of human immunoglobulin IgG1. The EPO molecule may be linked directly to the Fc fragment to avoid extraneous peptide linkers and lessen the risk of an immunogenic response when administered in vivo. In one embodiment the hinge region is a human Fc fragment variant having a non-cysteine residue at amino acid 6. The invention also relates to nucleic acid and amino acid sequences encoding the fusion protein and transfected cell lines and methods for producing the fusion protein. The invention further includes pharmaceutical compositions comprising the fusion protein and methods of using the fusion protein and/or the pharmaceutical compositions, for example to stimulate erythropoiesis in subjects in need of therapy.

摘要翻译： 描述了包含与免疫球蛋白肽部分连接的人促红细胞生成素肽部分的重组融合蛋白。 与天然存在的或重组的天然人促红细胞生成素相比，融合蛋白在体内具有延长的半衰期。 在本发明的一个实施方案中，蛋白质的体内半衰期比天然人促红细胞生成素高至少三倍。 与天然人促红细胞生成素相比，融合蛋白也表现出增强的红细胞生物活性。 在一个实施方案中，融合蛋白包含人促红细胞生成素（EPO）分子的完整肽序列和人免疫球蛋白IgG1的Fc片段的肽序列。 融合蛋白中的Fc片段包括人免疫球蛋白IgG1的铰链区，CH2和CH3结构域。 EPO分子可以直接连接到Fc片段，以避免外来的肽接头并且当在体内施用时减轻免疫原性应答的风险。 在一个实施方案中，铰链区是在氨基酸6具有非半胱氨酸残基的人Fc片段变体。本发明还涉及编码融合蛋白和转染细胞系的核酸和氨基酸序列以及用于产生融合蛋白的方法。 本发明还包括包含融合蛋白的药物组合物和使用融合蛋白和/或药物组合物的方法，例如刺激需要治疗的受试者的红细胞生成。

公开(公告)号：US20070109336A1

公开(公告)日：2007-05-17

申请号：US11271683

申请日：2005-11-11

申请人： Du-Yong Ng

发明人： Du-Yong Ng

IPC分类号： B41J2/205

CPC分类号： H04N1/40081 ,  G06K15/02

摘要： A method for reducing an amount of ink used in printing an image includes processing data representing the image to distinguish each halftone region from each solid region, wherein each solid region is formed by boundary ON pixels connected to interconnected interior ON pixels and having a sum of a quantity of the boundary ON pixels and a quantity of the interior ON pixels satisfying a predetermined threshold quantity; performing halftoning on the interior ON pixels of each solid region to form a reduced number of interior ON pixels to achieve a desired gray scale level; and printing the image consistent with the halftone region(s), and consistent with the boundary ON pixels and the reduced number of interior ON pixels representing each solid region.

摘要翻译： 用于减少打印图像中使用的墨水量的方法包括处理表示图像的数据以区分每个半色调区域与每个固体区域，其中每个固体区域由连接到互连的内部ON像素的边界ON像素形成，并且具有 边界ON像素的数量和满足预定阈值的内部ON像素的量; 在每个固体区域的内部ON像素上执行半色调以形成减少数量的内部ON像素以实现期望的灰度级; 并打印与半色调区域一致的图像，并且与边界ON像素和减少的表示每个固体区域的内部ON像素的数量一致。

公开(公告)号：US20070002375A1

公开(公告)日：2007-01-04

申请号：US11170949

申请日：2005-06-30

申请人： Du-Yong Ng

发明人： Du-Yong Ng

IPC分类号： G06K15/00

CPC分类号： H04N1/3878 ,  G06K9/00456 ,  G06K9/32

摘要： A method of segmenting and aligning a plurality of cards in a multi-card image, each card of the plurality of cards having at least one object, the multi-card image having a plurality of the objects, includes determining which pixels of the multi-card image are content pixels; grouping together a plurality of the content pixels corresponding to each object of the plurality of the objects to form a cluster corresponding to the each object, the grouping performed for the plurality of the objects to create a plurality of clusters corresponding to the plurality of the objects; determining which clusters of the plurality of clusters should be joined together to form a plurality of superclusters; and forming the plurality of superclusters, each supercluster of the plurality of superclusters corresponding to one card of the plurality of cards.

摘要翻译： 一种在多卡片图像中分割和对齐多个卡片的方法，所述多张卡片中的每个卡片具有至少一个对象，所述多张卡片图像具有多个对象，包括确定所述多张卡片中的哪些像素， 卡片图像是内容像素; 将与多个对象中的每个对象相对应的多个内容像素组合在一起以形成与每个对象对应的群集，为多个对象执行分组以创建与多个对象相对应的多个群集 ; 确定所述多个群集中的哪些群集应该被联接在一起以形成多个超群集; 以及形成所述多个超级群集，所述多个超级群集中的每个超群集对应于所述多个卡中的一张卡。

公开(公告)号：US20060227382A1

公开(公告)日：2006-10-12

申请号：US11095110

申请日：2005-03-31

申请人： Du-yong Ng ,  Khageshwar Thakur

发明人： Du-yong Ng ,  Khageshwar Thakur

IPC分类号： H04N1/409

CPC分类号： H04N1/40075

摘要： A method for descreening a scanned image includes providing an image in a color space. A target pixel is identified from a plurality of original pixels in the color space. An average value and a standard deviation value is computed for the target pixel with respect to other pixels in a sample window for each channel of the color space. For each channel in the color space an associated lookup table storing a plurality of kernel size values is selected. The average value and the standard deviation value computed for each channel is used to index the associated lookup table to select a kernel size value of the plurality of kernel size values for each channel. The kernel size value for each channel is applied to a low-pass filter to generate filtered pixel values in the color space corresponding to the target pixel, and the target pixel values of the target pixel are replaced with the filtered pixel values.

摘要翻译： 用于对扫描图像进行去网格化的方法包括在颜色空间中提供图像。 从颜色空间中的多个原始像素识别目标像素。 相对于颜色空间的每个通道的样本窗口中的其他像素，针对目标像素计算平均值和标准偏差值。 对于颜色空间中的每个通道，选择存储多个内核大小值的相关联的查找表。 为每个通道计算的平均值和标准偏差值用于对相关联的查找表进行索引，以选择每个通道的多个内核大小值的内核大小值。 将每个通道的内核大小值应用于低通滤波器，以在与目标像素对应的颜色空间中生成滤波像素值，并且将目标像素的目标像素值替换为滤波像素值。

公开(公告)号：US08431132B2

公开(公告)日：2013-04-30

申请号：US12555743

申请日：2009-09-08

申请人： Haitao Wang ,  Du Yong ,  Rui Zhang ,  Jing Xu ,  Longbin Liu

发明人： Haitao Wang ,  Du Yong ,  Rui Zhang ,  Jing Xu ,  Longbin Liu

IPC分类号： A61K39/00 ,  C07K1/00 ,  C07K16/00

CPC分类号： C07K14/505 ,  A61K9/0019 ,  A61K38/00 ,  A61K47/6811 ,  C07K2317/53 ,  C07K2319/30

摘要： A recombinant fusion protein comprising a human erythropoietin peptide portion linked to an immunoglobulin peptide portion is described. The fusion protein has a prolonged half-life in vivo in comparison to naturally occurring or recombinant native human erythropoietin. In one embodiment of the invention, the protein has a half-life in vivo at least three fold higher than native human erythropoietin. The fusion protein also exhibits enhanced erythropoietic bioactivity in comparison to native human erythropoietin. In one embodiment, the fusion protein comprises the complete peptide sequence of a human erythropoietin (EPO) molecule and the peptide sequence of an Fc fragment of human immunoglobulin IgG1. The Fc fragment in the fusion protein includes the hinge region, CH2 and CH3 domains of human immunoglobulin IgG1. The EPO molecule may be linked directly to the Fc fragment to avoid extraneous peptide linkers and lessen the risk of an immunogenic response when administered in vivo. In one embodiment the hinge region is a human Fc fragment variant having a non-cysteine residue at amino acid 6. The invention also relates to nucleic acid and amino acid sequences encoding the fusion protein and transfected cell lines and methods for producing the fusion protein. The invention further includes pharmaceutical compositions comprising the fusion protein and methods of using the fusion protein and/or the pharmaceutical compositions, for example to stimulate erythropoiesis in subjects in need of therapy.

摘要翻译： 描述了包含与免疫球蛋白肽部分连接的人促红细胞生成素肽部分的重组融合蛋白。 与天然存在的或重组的天然人促红细胞生成素相比，融合蛋白在体内具有延长的半衰期。 在本发明的一个实施方案中，蛋白质的体内半衰期比天然人促红细胞生成素高至少三倍。 与天然人促红细胞生成素相比，融合蛋白也表现出增强的红细胞生物活性。 在一个实施方案中，融合蛋白包含人促红细胞生成素（EPO）分子的完整肽序列和人免疫球蛋白IgG1的Fc片段的肽序列。 融合蛋白中的Fc片段包括人免疫球蛋白IgG1的铰链区，CH2和CH3结构域。 EPO分子可以直接连接到Fc片段，以避免外来的肽接头并且当在体内施用时减轻免疫原性应答的风险。 在一个实施方案中，铰链区是在氨基酸6具有非半胱氨酸残基的人Fc片段变体。本发明还涉及编码融合蛋白和转染细胞系的核酸和氨基酸序列以及用于产生融合蛋白的方法。 本发明还包括包含融合蛋白的药物组合物和使用融合蛋白和/或药物组合物的方法，例如刺激需要治疗的受试者的红细胞生成。

公开(公告)号：US07964375B2

公开(公告)日：2011-06-21

申请号：US12555742

申请日：2009-09-08

申请人： Haitao Wang ,  Du Yong ,  Rui Zhang ,  Jing Xu ,  Longbin Liu

发明人： Haitao Wang ,  Du Yong ,  Rui Zhang ,  Jing Xu ,  Longbin Liu

IPC分类号： A61K39/00 ,  C12P21/06 ,  C12N1/20 ,  C12N15/00 ,  C07K14/00 ,  C07H21/02

CPC分类号： C07K14/505 ,  A61K9/0019 ,  A61K38/00 ,  A61K47/6811 ,  C07K2317/53 ,  C07K2319/30

摘要： A recombinant fusion protein comprising a human erythropoietin peptide portion linked to an immunoglobulin peptide portion is described. The fusion protein has a prolonged half-life in vivo in comparison to naturally occurring or recombinant native human erythropoietin. In one embodiment of the invention, the protein has a half-life in vivo at least three fold higher than native human erythropoietin. The fusion protein also exhibits enhanced erythropoietic bioactivity in comparison to native human erythropoietin. In one embodiment, the fusion protein comprises the complete peptide sequence of a human erythropoietin (EPO) molecule and the peptide sequence of an Fc fragment of human immunoglobulin IgG1. The Fc fragment in the fusion protein includes the hinge region, CH2 and CH3 domains of human immunoglobulin IgG1. The EPO molecule may be linked directly to the Fc fragment to avoid extraneous peptide linkers and lessen the risk of an immunogenic response when administered in vivo. In one embodiment the hinge region is a human Fc fragment variant having a non-cysteine residue at amino acid 6. The invention also relates to nucleic acid and amino acid sequences encoding the fusion protein and transfected cell lines and methods for producing the fusion protein. The invention further includes pharmaceutical compositions comprising the fusion protein and methods of using the fusion protein and/or the pharmaceutical compositions, for example to stimulate erythropoiesis in subjects in need of therapy.

摘要翻译： 描述了包含与免疫球蛋白肽部分连接的人促红细胞生成素肽部分的重组融合蛋白。 与天然存在的或重组的天然人促红细胞生成素相比，融合蛋白在体内具有延长的半衰期。 在本发明的一个实施方案中，蛋白质的体内半衰期比天然人促红细胞生成素高至少三倍。 与天然人促红细胞生成素相比，融合蛋白也表现出增强的红细胞生物活性。 在一个实施方案中，融合蛋白包含人促红细胞生成素（EPO）分子的完整肽序列和人免疫球蛋白IgG1的Fc片段的肽序列。 融合蛋白中的Fc片段包括人免疫球蛋白IgG1的铰链区，CH2和CH3结构域。 EPO分子可以直接连接到Fc片段，以避免外来的肽接头并且当在体内施用时减轻免疫原性应答的风险。 在一个实施方案中，铰链区是在氨基酸6具有非半胱氨酸残基的人Fc片段变体。本发明还涉及编码融合蛋白和转染细胞系的核酸和氨基酸序列以及用于产生融合蛋白的方法。 本发明还包括包含融合蛋白的药物组合物和使用融合蛋白和/或药物组合物的方法，例如刺激需要治疗的受试者的红细胞生成。

公开(公告)号：US07453587B2

公开(公告)日：2008-11-18

申请号：US11295959

申请日：2005-12-07

申请人： William Everett Gardner ,  Du-Yong Ng

发明人： William Everett Gardner ,  Du-Yong Ng

IPC分类号： G06F15/00 ,  G06K1/00 ,  H04N1/38 ,  G06F3/12

CPC分类号： G06K9/40 ,  H04N1/4097

摘要： A method for removing streaks from a scanned image includes performing a scanner light source OFF scan to generate a dark image having M rows of pixels and N+W columns of pixels; generating a line noise profile for the N columns of pixels of the dark image; performing a scanner light source ON scan to generate a regular image having M rows of pixels and N+W columns of pixels; processing the W columns of pixels of the regular image to detect a magnitude of the streak in the regular image; use the magnitude of the streak and the line noise profile to predict an amount of dark noise removal for each of the N columns of pixels of the regular image at the location of the streak; and subtracting the predicted amount of dark noise removal for each of the N columns of pixels from the respective N columns of pixels of the regular image.

摘要翻译： 用于从扫描图像中去除条纹的方法包括执行扫描仪光源OFF扫描以产生具有M行像素和N + W列像素的暗图像; 生成暗图像的N列像素的线噪声分布; 执行扫描仪光源ON扫描以产生具有M行像素和N + W列像素的规则图像; 处理常规图像的W列的像素以检测正常图像中的条纹的大小; 使用条纹和线噪声分布的大小来预测在条纹位置处的常规图像的N列像素中的每一个的暗噪声去除量; 并从所述常规图像的各N列像素中减去所述N列像素中的每一个的暗噪声去除的预测量。

公开(公告)号：US20070076265A1

公开(公告)日：2007-04-05

申请号：US11242487

申请日：2005-10-03

申请人： William Gardner ,  Du-Yong Ng ,  John Pospisil

发明人： William Gardner ,  Du-Yong Ng ,  John Pospisil

IPC分类号： H04N1/04

CPC分类号： H04N1/407 ,  H04N1/6027 ,  H04N1/6077

摘要： A method of bit depth reduction for an apparatus includes establishing a human visual response versus relative luminance, the human visual response being defined by 2M levels; determining a scanner response versus the relative luminance for at least one channel of scanner data, the scanner response being represented by N-bit per channel data, wherein N is greater than M; relating the human visual response to the scanner response; and quantizing the N-bit per channel data to M-bit per channel data according to the human visual response.

摘要翻译： 一种用于装置的位深度降低的方法包括建立人类视觉响应与相对亮度，人类视觉响应由2级水平定义; 确定扫描器响应相对于扫描器数据的至少一个通道的相对亮度，扫描器响应由每通道数据N位表示，其中N大于M; 将人类视觉响应与扫描器响应相关联; 并且根据人类视觉响应将每通道数据的N位量化到每通道数据M位。

公开(公告)号：US20070274607A1

公开(公告)日：2007-11-29

申请号：US11279445

申请日：2006-04-12

申请人： Jincheng Huang ,  Du-Yong Ng

发明人： Jincheng Huang ,  Du-Yong Ng

IPC分类号： G06K9/36

CPC分类号： G06T15/506

摘要： A method of creating a reflection effect in an image includes choosing an observer viewpoint; interpolating selected pixels in the image to generate reflection pixels based on the observer viewpoint; and generating a reconstructed image using the selected pixels and the reflection pixels to create the reflection effect.

摘要翻译： 在图像中产生反射效果的方法包括选择观察者视点; 内插图像中的所选像素以根据观察者视点产生反射像素; 以及使用所选择的像素和反射像素产生重建图像以产生反射效果。