-
公开(公告)号：US20170176458A1
公开(公告)日：2017-06-22
申请号：US15446070
申请日：2017-03-01
申请人： Sanne S. Veidal , Morten A. Karsdal , Diana J. Oersnes-Leeming , Natasha Barascuk Michaelsen , Helene Skjot-Arkil , Antonio Segovia-Silvestre , Efstathios Vassiliadis
发明人： Sanne S. Veidal , Morten A. Karsdal , Diana J. Oersnes-Leeming , Natasha Barascuk Michaelsen , Helene Skjot-Arkil , Antonio Segovia-Silvestre , Efstathios Vassiliadis
IPC分类号： G01N33/68
CPC分类号： G01N33/6893 , C12Q1/37 , G01N2333/4737 , G01N2333/78 , G01N2800/085 , G01N2800/32 , G01N2800/50
摘要： Methods of diagnosis or of quantitation of fibrosis are provided that comprise conducting an immunoassay to measure neo-epitope containing protein fragments naturally present in a bioflui sample, and associating an elevation of the measure in the patient above a normal level with the presence or extent of fibrosis. The immunoassay is conducted by a contacting protein fragments naturally present in the sample with an immunological binding partner reactive with a neo-epitope formed by cleavage of a protein by a proteinase and measuring the extent of binding of peptide fragments to the immunological binding partner to measure therein protein fragments comprising the neo-epitope. The protein is collagen type I, collagen type IV, collagen type V, elastin, biglycan, decorin, lumican, versican, perlecan, neurocan, brevican, fibromodulin, serglycin, syndecan, betaglycan, vimentin, or C-reactive protein.
-
公开(公告)号：US09404932B2
公开(公告)日：2016-08-02
申请号：US13187205
申请日：2011-07-20
申请人： Sanne S. Veidal , Morten A. Karsdal , Diana J. Leeming , Natasha Barascuk , Helene Skjøt-Arkil , Efstathios Vassiliadis
发明人： Sanne S. Veidal , Morten A. Karsdal , Diana J. Leeming , Natasha Barascuk , Helene Skjøt-Arkil , Efstathios Vassiliadis
CPC分类号： G01N33/6893 , C07K14/78 , C12Q1/37 , G01N2333/4737 , G01N2333/775 , G01N2333/78 , G01N2800/32
摘要： Methods of diagnosis or of quantitation of pathological conditions comprise conducting an immunoassay to measure neo-epitope containing protein fragments naturally present in a biofluid sample, and associating an elevation of the measure in the patient above a normal level with the presence or extent of pathology. The immunoassay is conducted by a method comprising: contacting protein fragments naturally present in the sample with an immunological binding partner reactive with a neo-epitope formed by cleavage of a protein by a proteinase and measuring the extent of binding of peptide fragments to the immunological binding partner to measure therein protein fragments comprising the neo-epitope. Neo-epitopes from, collagen type I, collagen type III, collagen type IV, collagen type V, collagen type VI, elastin, biglycan, decorin, lumican, versican, C-reactive protein, ApoE and laminins are described.
摘要翻译： 诊断或定量的病理状况的方法包括进行免疫测定以测量天然存在于生物流体样品中的含有蛋白质片段的蛋白质片段，并将患者的标准物高于常规水平与病理学的存在或程度相关联。 通过以下方法进行免疫测定：将天然存在于样品中的蛋白质片段与免疫结合配偶体接触，所述免疫结合配偶体与通过蛋白酶切割蛋白质形成的新表位反应，并测量肽片段与免疫结合的程度 在其中测量包含新表位的蛋白质片段的伴侣。 描述了I型胶原，III型胶原，IV型胶原，V型胶原蛋白，弹性蛋白，双聚糖，核心蛋白聚糖，多巴胺，多巴胺，C反应蛋白，ApoE和层粘连蛋白的新表位。
-
公开(公告)号：US09459260B2
公开(公告)日：2016-10-04
申请号：US14122349
申请日：2012-05-23
IPC分类号： G01N31/00 , G01N33/53 , G01N33/68 , G01N33/564 , C07K16/18
CPC分类号： G01N33/6887 , C07K16/18 , G01N33/564 , G01N33/6842 , G01N2333/78 , G01N2800/102
摘要： An assay for citrullinated fragments of SOCS-2, Alpha 1 anti tyrpsin, versican, biglycan, laminin, or other protein having a terminal antibody binding site comprising citrulline in a blood derived sample shows diagnostic relevance in relation to rheumatoid arthritis or fibrotic disease.
摘要翻译： 在血液衍生的样品中具有包含瓜氨酸的末端抗体结合位点的SOCS-2，α1抗酪氨酸蛋白酶，多核苷酸，二聚糖，层粘连蛋白或其他蛋白质的瓜氨酸化片段的测定显示与类风湿性关节炎或纤维化疾病有关的诊断意义。
-
4.发明申请In Vitro Assessment of Cardiovascular Events by Assay for Neo-Eptitopes of Titin Protein 有权通过测定蛋白质新蛋白的体外评估心血管事件公开(公告)号：US20140295469A1
公开(公告)日：2014-10-02
申请号：US14232405
申请日：2012-07-06
CPC分类号： G01N33/6887 , C07K14/4716 , C07K16/18 , C07K2317/34 , G01N33/566 , G01N33/6893 , G01N2333/4712 , G01N2800/32
摘要： A bioassay for the quantification of peptide fragments comprising a neo-epitope formed by cleavage of a titin protein by a proteinase comprises contacting a sample with an antibody specifically binding said neo-epitope and determining the level of binding. Partial sequences of titin that may be detected include: 13519′ ↓GEYVCDCGTD ′13528 13532′ NVTVEARLIK↓ ′13542 13543′ ↓VEKPLYGVEV ′1553
摘要翻译： 用于定量肽片段的生物测定法，其包含通过蛋白酶切割titin蛋白质而形成的新表位，包括使样品与特异性结合所述新表位的抗体接触并确定结合水平。 可能检测到的部分滴度的序列包括：13519'↓GEYVCDCGTD'13528 13532'NVTVEARLIK↓'13542 13543'↓VEKPLYGVEV'1553
-
公开(公告)号：US20100209940A1
公开(公告)日：2010-08-19
申请号：US12749652
申请日：2010-03-30
申请人： Sanne S. Veidal , Morten A. Karsdal , Diana J. Leeming , Natasha Barascuk , Helene Skjøt-Arkil , Antonio Segovia-Silvestre , Efstathios Vassiliadis
发明人： Sanne S. Veidal , Morten A. Karsdal , Diana J. Leeming , Natasha Barascuk , Helene Skjøt-Arkil , Antonio Segovia-Silvestre , Efstathios Vassiliadis
IPC分类号： G01N33/53
CPC分类号： G01N33/6893 , C12Q1/37 , G01N2333/4737 , G01N2333/78 , G01N2800/085 , G01N2800/32 , G01N2800/50
摘要： Methods of diagnosis or of quantitation of fibrosis comprise conducting an immunoassay to measure neo-epitope containing protein fragments naturally present in a biofluid sample, and associating an elevation of said measure in said patient above a normal level with the presence or extent of fibrosis. The immunoassay is conducted by a method comprising:contacting protein fragments naturally present in said sample with an immunological binding partner reactive with a neo-epitope formed by cleavage of a protein by a proteinase and measuring the extent of binding of peptide fragments to said immunological binding partner to measure therein protein fragments comprising said neo-epitope, and wherein said protein is collagen type III, collagen type I, collagen type IV, collagen type V, or collagen type VI, elastin, biglycan, decorin, lumican, versican, perlecan, neurocan, brevican, fibromodulin, serglycin, syndecan, betaglycan, vimentin, or C-reactive protein.
摘要翻译： 诊断或定量纤维化的方法包括进行免疫测定以测量天然存在于生物流体样品中的含有蛋白质片段的蛋白质片段，并将所述患者中所述患者的高度高于正常水平与纤维化的存在或程度相关联。 免疫测定通过以下方法进行，该方法包括：将天然存在于所述样品中的蛋白质片段与免疫结合配偶体接触，所述免疫结合配偶体与通过蛋白酶切割蛋白质形成的新表位反应，并测量肽片段与所述免疫结合的结合程度 其中所述蛋白质是III型胶原，I型胶原，IV型胶原，V型胶原或VI型胶原蛋白，弹性蛋白，双糖链蛋白聚糖，核心蛋白聚糖，多巴胺，多聚核苷酸， 神经鞘蛋白，短纤维蛋白原，纤维调节蛋白，血清蛋白，syndecan，betaglycan，波形蛋白或C反应蛋白。
-
公开(公告)号：US20160091502A1
公开(公告)日：2016-03-31
申请号：US14960713
申请日：2015-12-07
申请人： Sanne S. Veidal , Morten A. Karsdal , Diana J. Oersnes-Leeming , Natasha Barascuk Michaelsen , Helene Skjot-Arkil , Antonio Segovia-Silvestre , Efstathios Vassiliadis
发明人： Sanne S. Veidal , Morten A. Karsdal , Diana J. Oersnes-Leeming , Natasha Barascuk Michaelsen , Helene Skjot-Arkil , Antonio Segovia-Silvestre , Efstathios Vassiliadis
IPC分类号： G01N33/68
CPC分类号： G01N33/6893 , C12Q1/37 , G01N2333/4737 , G01N2333/78 , G01N2800/085 , G01N2800/32 , G01N2800/50
摘要： Methods of diagnosis or of quantitation of fibrosis are provided that comprise conducting an immunoassay to measure neo-epitope containing protein fragments naturally present in a biofluid sample, and associating an elevation of the measure in the patient above a normal level with the presence or extent of fibrosis. The immunoassay is conducted by a contacting protein fragments naturally present in the sample with an immunological binding partner reactive with a neo-epitope formed by cleavage of a protein by a proteinase and measuring the extent of binding of peptide fragments to the immunological binding partner to measure therein protein fragments comprising the neo-epitope. The protein is collagen type III, collagen type I, collagen type IV, collagen type V, elastin, biglycan, decorin, lumican, versican, perlecan, neurocan, brevican, fibromodulin, serglycin, syndecan, betaglycan, vimentin, or C-reactive protein.
摘要翻译： 提供了诊断或定量纤维化的方法，其包括进行免疫测定以测量天然存在于生物流体样品中的含有蛋白质片段的蛋白质片段，并将患者高于正常水平的措施的升高与存在或程度相关联 纤维化。 免疫测定通过天然存在于样品中的接触蛋白片段与免疫结合配偶体进行，所述蛋白质片段与通过蛋白酶切割蛋白质形成的新表位反应，并测量肽片段与免疫结合配偶体的结合程度以测量 其中包含新表位的蛋白质片段。 蛋白质是III型胶原，I型胶原，IV型胶原，弹性蛋白，双聚糖蛋白聚糖，核心蛋白聚糖，多西紫杉醇，多西紫杉醇，神经鞘蛋白，短纤维蛋白，纤维调节蛋白，丝氨酸蛋白酶，syndecan，betaglycan，波形蛋白或C-反应蛋白 。
-
公开(公告)号：US09206464B2
公开(公告)日：2015-12-08
申请号：US12749652
申请日：2010-03-30
申请人： Sanne S. Veidal , Morten A. Karsdal , Diana J. Leeming , Natasha Barascuk , Helene Skjot-Arkil , Antonio Segovia-Silvestre , Efstathios Vassiliadis
发明人： Sanne S. Veidal , Morten A. Karsdal , Diana J. Leeming , Natasha Barascuk , Helene Skjot-Arkil , Antonio Segovia-Silvestre , Efstathios Vassiliadis
CPC分类号： G01N33/6893 , C12Q1/37 , G01N2333/4737 , G01N2333/78 , G01N2800/085 , G01N2800/32 , G01N2800/50
摘要： Provided herein are methods of diagnosis or of quantitation of fibrosis. An immunoassay is conducted to measure neo-epitope containing protein fragments of collagen type III, collagen type I, collagen type IV, collagen type V, or collagen type VI, elastin, biglycan, decorin, lumican, versican, perlecan, neurocan, brevican, fibromodulin, serglycin, syndecan, betaglycan, vimentin, or C-reactive protein naturally present in a biofluid sample obtained from a patient. An above normal elevation of the measured protein fragments in the patient is associated with the presence or extent of fibrosis.
摘要翻译： 本文提供了诊断或定量纤维化的方法。 进行免疫测定以测量含有III型胶原蛋白，I型胶原，IV型胶原，V型胶原或VI型胶原蛋白，弹性蛋白，双糖链蛋白聚糖，核心蛋白聚糖，多巴胺，多西紫杉醇，神经氨酸， 纤维调节素，丝氨酸蛋白酶，syndecan，betaglycan，波形蛋白或C反应蛋白，其天然存在于从患者获得的生物流体样品中。 患者中测量的蛋白质片段的正常升高与纤维化的存在或程度相关。
-
公开(公告)号：US20120045781A1
公开(公告)日：2012-02-23
申请号：US13187205
申请日：2011-07-20
申请人： Sanne S. Veidal , Morten A. Karsdal , Diana J. Leeming , Natasha Barascuk , Helene Skjøt-Arkil , Efstathios Vassiliadis
发明人： Sanne S. Veidal , Morten A. Karsdal , Diana J. Leeming , Natasha Barascuk , Helene Skjøt-Arkil , Efstathios Vassiliadis
IPC分类号： G01N33/566
CPC分类号： G01N33/6893 , C07K14/78 , C12Q1/37 , G01N2333/4737 , G01N2333/775 , G01N2333/78 , G01N2800/32
摘要： Methods of diagnosis or of quantitation of pathological conditions comprise conducting an immunoassay to measure neo-epitope containing protein fragments naturally present in a biofluid sample, and associating an elevation of said measure in said patient above a normal level with the presence or extent of pathology. The immunoassay is conducted by a method comprising: contacting protein fragments naturally present in said sample with an immunological binding partner reactive with a neo-epitope formed by cleavage of a protein by a proteinase and measuring the extent of binding of peptide fragments to said immunological binding partner to measure therein protein fragments comprising said neo-epitope. Neo-epitopes from, collagen type I, collagen type III, collagen type IV, collagen type V, collagen type VI, elastin, biglycan, decorin, lumican, versican, C-reactive protein, ApoE and laminins are described.
摘要翻译： 诊断或定量的病理状况的方法包括进行免疫测定以测量天然存在于生物流体样品中的含有蛋白质片段的蛋白质片段，并将所述患者中所述患者的高度高于正常水平与病理学的存在或程度相关联。 免疫测定通过以下方法进行，该方法包括：将天然存在于所述样品中的蛋白质片段与免疫结合配偶体接触，所述免疫结合配偶体与通过蛋白酶切割蛋白质形成的新表位反应，并测量肽片段与所述免疫结合的结合程度 伴侣在其中测量包含所述新表位的蛋白质片段。 描述了I型胶原，III型胶原，IV型胶原，V型胶原蛋白，弹性蛋白，双聚糖，核心蛋白聚糖，多巴胺，多巴胺，C反应蛋白，ApoE和层粘连蛋白的新表位。
-
公开(公告)号：US09880177B2
公开(公告)日：2018-01-30
申请号：US15446070
申请日：2017-03-01
申请人： Sanne S. Veidal , Morten A. Karsdal , Diana J. Oersnes-Leeming , Natasha Barascuk Michaelsen , Helene Skjot-Arkil , Antonio Segovia-Silvestre , Efstathios Vassiliadis
发明人： Sanne S. Veidal , Morten A. Karsdal , Diana J. Oersnes-Leeming , Natasha Barascuk Michaelsen , Helene Skjot-Arkil , Antonio Segovia-Silvestre , Efstathios Vassiliadis
CPC分类号： G01N33/6893 , C12Q1/37 , G01N2333/4737 , G01N2333/78 , G01N2800/085 , G01N2800/32 , G01N2800/50
摘要： A method of bioassay for the quantification of biomarkers useful in the diagnosis of fibrosis disease and prognosis of its development, including biomarkers indicative of the risk of developing fibrosis after a chronic injury is provided. In particular, according to the present invention, biomarkers relating to degradation fragments of Collagen type I, III, IV, V, and VI, elastin, C-reactive protein, and proteoglycans including Biglycan, Decorin, Versican, and Perlecan are found to be useful.
-
公开(公告)号：US09606130B2
公开(公告)日：2017-03-28
申请号：US14960713
申请日：2015-12-07
申请人： Sanne S. Veidal , Morten A. Karsdal , Diana J. Oersnes-Leeming , Natasha Barascuk Michaelsen , Helene Skjot-Arkil , Antonio Segovia-Silvestre , Efstathios Vassiliadis
发明人： Sanne S. Veidal , Morten A. Karsdal , Diana J. Oersnes-Leeming , Natasha Barascuk Michaelsen , Helene Skjot-Arkil , Antonio Segovia-Silvestre , Efstathios Vassiliadis
CPC分类号： G01N33/6893 , C12Q1/37 , G01N2333/4737 , G01N2333/78 , G01N2800/085 , G01N2800/32 , G01N2800/50
摘要： Provided herein are methods of diagnosis or of quantitation of fibrosis. An immunoassay is conducted to measure neo-epitope containing protein fragments of collagen type III, collagen type I, collagen type IV, collagen type V, or collagen type VI, elastin, biglycan, decorin, lumican, versican, perlecan, neurocan, brevican, fibromodulin, serglycin, syndecan, betaglycan, vimentin, or C-reactive protein naturally present in a biofluid sample obtained from a patient. An above normal elevation of the measured protein fragments in the patient is associated with the presence or extent of fibrosis.
-
-
-
-
-
-
-
-
-
搜索结果
11 条记录 (耗时 0.026 秒)
