公开(公告)号：US20120202976A1

公开(公告)日：2012-08-09

申请号：US13501623

申请日：2010-10-08

申请人： Andreas Axen ,  Eggert Brekkan ,  Gunilla Edgren ,  Gunnar Malmquist

发明人： Andreas Axen ,  Eggert Brekkan ,  Gunilla Edgren ,  Gunnar Malmquist

IPC分类号： C07K1/14 ,  C08B37/00 ,  C08B37/02

CPC分类号： B01J20/289 ,  B01D15/3847 ,  B01J20/287 ,  B01J20/3285 ,  B01J39/19 ,  B01J39/26 ,  B01J41/13 ,  B01J41/20 ,  B01D15/327 ,  B01D15/362 ,  B01D15/363

摘要： The present invention relates to separation matrices comprising base matrices with first ligands comprising hydrophobic functions covalently bound to said base matrices and with extenders covalently bound to said base matrices, said extenders comprising second ion exchange ligands.

摘要翻译： 本发明涉及分离基质，其包含具有第一配体的基质基质，所述第一配体包含与所述基础基质共价结合的疏水官能团和与所述基础基质共价结合的延伸剂，所述增量剂包含第二离子交换配体。

公开(公告)号：US09944722B2

公开(公告)日：2018-04-17

申请号：US13701074

申请日：2011-05-30

申请人： Mattias Algotsson ,  Lars C. Andersson ,  Eggert Brekkan ,  Lee Hedemyr ,  Jean-Luc Maloisel ,  Ronnie Palmgren

发明人： Mattias Algotsson ,  Lars C. Andersson ,  Eggert Brekkan ,  Lee Hedemyr ,  Jean-Luc Maloisel ,  Ronnie Palmgren

IPC分类号： C07C237/10 ,  C08B37/00 ,  B01J20/289 ,  B01J20/32 ,  C07C323/60 ,  B01J20/281

CPC分类号： C08B37/0039 ,  B01J20/281 ,  B01J20/289 ,  B01J20/3219 ,  B01J20/3251 ,  B01J20/3265 ,  C07C237/10 ,  C07C323/60

摘要： The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.The dimeric pentadentate chelator according to the invention has the following general formula: wherein Sc is a scaffold or a connecting structure that contains at least two functional groups enabling coupling of two pentadentate chelators (PD), and PD is a pentadentate chelator having the formula:

公开(公告)号：US20130072638A1

公开(公告)日：2013-03-21

申请号：US13701074

申请日：2011-05-30

申请人： Mattias Algotsson ,  Lars C. Andersson ,  Eggert Brekkan ,  Lee Hedemyr ,  Jean-Luc Maloisel ,  Ronnie Palmgren

发明人： Mattias Algotsson ,  Lars C. Andersson ,  Eggert Brekkan ,  Lee Hedemyr ,  Jean-Luc Maloisel ,  Ronnie Palmgren

IPC分类号： C08B37/00 ,  B01J20/281

CPC分类号： C08B37/0039 ,  B01J20/281 ,  B01J20/289 ,  B01J20/3219 ,  B01J20/3251 ,  B01J20/3265 ,  C07C237/10 ,  C07C323/60

摘要： The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.The dimeric pentadentate chelator according to the invention has the following general formula: whereinSc is a scaffold or a connecting structure that contains at least two functional groups enabling coupling of two pentadentate chelators (PD), andPD is a pentadentate chelator having the formula:

摘要翻译： 本发明涉及具有非常强的金属离子结合的二聚五齿螯合物，用于生物分子的检测，固定和纯化。 二聚螯合剂提供了两个相邻的固定化金属离子与组氨酸标记的生物分子的结合的协同作用，与对应的单体螯合剂相比，其对结合强度具有有利的性质。 此外，二聚体增加了具有低金属离子亲和力的非标记生物分子的选择性（易于分离）。 根据本发明的二聚五齿螯合剂具有以下通式：其中Sc是含有至少两个能够偶合两种五齿螯合剂（PD）的官能团的支架或连接结构，PD是具有下式的五齿螯合剂：