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公开(公告)号：US09989446B2
公开(公告)日：2018-06-05
申请号：US13985360
申请日：2012-02-27
申请人： Philip Robert Mallender , Douglas Mark Green , Sarah Janet Clegg , Barry Edmund Johnson , Jimmy Hedin Dahlstrom , Mattias Algotsson , Ronnie Palmgren
发明人： Philip Robert Mallender , Douglas Mark Green , Sarah Janet Clegg , Barry Edmund Johnson , Jimmy Hedin Dahlstrom , Mattias Algotsson , Ronnie Palmgren
摘要： The invention discloses a sample preservation method which comprises the following steps: a) providing a paper substrate comprising ligands, where the ligands comprise charged groups, b) applying a sample comprising at least one analyte and at least one contaminant on the paper substrate, c) drying the sample on the paper substrate and, d) extracting at least part of the paper substrate to provide a solution of the analyte.
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公开(公告)号：US07060776B2
公开(公告)日：2006-06-13
申请号：US10499884
申请日：2002-12-17
IPC分类号： C08F226/02
CPC分类号： C07C233/20 , C08F20/56 , G01N27/44747
摘要： The invention relates to an acrylic monomer defined by formula (I): wherein R1, R2 and R3 are H or CH3; R4 and R5 are H or OH; R6 and R7 are H or OH; R8 is H or CH3; m is 2–4; and n and p are 0 or 1. The invention also relates to a polymer comprised of repeated such monomer units as well as a separation matrix prepared thereof.
摘要翻译： 本发明涉及由式（I）定义的丙烯酸单体：其中R 1，R 2和R 3均为H或CH 3 ; R 4和R 5是H或OH; R 6和R 7是H或OH; R 8是H或CH 3; m为2-4; 并且n和p为0或1.本发明还涉及由重复的这种单体单元以及由其制备的分离基质组成的聚合物。
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公开(公告)号：US20070106090A1
公开(公告)日：2007-05-10
申请号：US10572294
申请日：2004-10-18
IPC分类号： C07C309/00
CPC分类号： C07C277/08 , C07C279/12
摘要： The present invention relates to a method for synthesis of an acrylamide derivative, starting with dissolving a salt of a nucleophilic amine in water to form an aqueous solution and desalting said solution with a base, comprising the following steps: a) addition of dissolved activated acrylic acid derivative to said solution; b) acidification of aqueous phase; and c) extraction of said aqueous phase. In a preferred embodiment the acryl amide derivative is an immobiline, preferably acrylamido agmatine. The preferred use of the immobilines is for production of 2D gels.
摘要翻译： 本发明涉及一种合成丙烯酰胺衍生物的方法，首先将亲核胺的盐溶解在水中形成水溶液，并用碱脱盐所述溶液，包括以下步骤：a）加入溶解的活化丙烯酸 酸衍生物至所述溶液; b）水相酸化; 和c）提取所述水相。 在优选的实施方案中，丙烯酰胺衍生物是固定化物，优选丙烯酰胺基丁胺。 固定装置的优选用途是生产2D凝胶。
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公开(公告)号：US20130072638A1
公开(公告)日：2013-03-21
申请号：US13701074
申请日：2011-05-30
申请人： Mattias Algotsson , Lars C. Andersson , Eggert Brekkan , Lee Hedemyr , Jean-Luc Maloisel , Ronnie Palmgren
发明人： Mattias Algotsson , Lars C. Andersson , Eggert Brekkan , Lee Hedemyr , Jean-Luc Maloisel , Ronnie Palmgren
IPC分类号： C08B37/00 , B01J20/281
CPC分类号： C08B37/0039 , B01J20/281 , B01J20/289 , B01J20/3219 , B01J20/3251 , B01J20/3265 , C07C237/10 , C07C323/60
摘要： The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.The dimeric pentadentate chelator according to the invention has the following general formula: whereinSc is a scaffold or a connecting structure that contains at least two functional groups enabling coupling of two pentadentate chelators (PD), andPD is a pentadentate chelator having the formula:
摘要翻译： 本发明涉及具有非常强的金属离子结合的二聚五齿螯合物，用于生物分子的检测，固定和纯化。 二聚螯合剂提供了两个相邻的固定化金属离子与组氨酸标记的生物分子的结合的协同作用，与对应的单体螯合剂相比，其对结合强度具有有利的性质。 此外，二聚体增加了具有低金属离子亲和力的非标记生物分子的选择性（易于分离）。 根据本发明的二聚五齿螯合剂具有以下通式：其中Sc是含有至少两个能够偶合两种五齿螯合剂（PD）的官能团的支架或连接结构，PD是具有下式的五齿螯合剂：
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公开(公告)号：US20100136647A1
公开(公告)日：2010-06-03
申请号：US12623528
申请日：2009-11-23
申请人： Mattias Algotsson , Hans Berg , Asa Bjurling , Christian Kaisermayer , Bjorn Noren , Nicholas Thevenin , James Van Alstine
发明人： Mattias Algotsson , Hans Berg , Asa Bjurling , Christian Kaisermayer , Bjorn Noren , Nicholas Thevenin , James Van Alstine
CPC分类号： C12N11/06 , C12N5/0075 , C12N7/00 , C12N11/00 , C12N11/10 , C12N11/12 , C12N2533/20 , C12N2710/00051
摘要： The present invention relates to the field of adherent cell culture. More closely, the invention relates to a method for production of a cell attachment and culture surface, such as a microcarrier, comprising a guanidino-containing ligand, wherein the ligand is coupled via reaction involving a primary amine to the surface which is activated by activation groups such that the final molar ratio of grafted ligand and ungrafted activation groups is above 1.5. Preferably, the ligand density is above 0.5 mmol/g cell culture surface and the remaining activation groups after coupling is less than 0.6 mmol/g cell culture surface. The cell culture surface may be used for various purposes, primarily cell cultivation and virus production.
摘要翻译： 本发明涉及贴壁细胞培养领域。 更详细地说，本发明涉及一种用于生产细胞附着和培养物表面的方法，例如微载体，其包含含胍基的配体，其中该配体通过涉及伯胺的反应与通过活化而被活化的表面偶联 使得接枝配体和未接枝的活化基团的最终摩尔比高于1.5。 优选地，配体密度高于0.5mmol / g细胞培养物表面，并且偶联后剩余的活化基团小于0.6mmol / g细胞培养物表面。 细胞培养物表面可以用于各种目的，主要是细胞培养和病毒生产。
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公开(公告)号：US07294743B2
公开(公告)日：2007-11-13
申请号：US10572294
申请日：2004-10-18
IPC分类号： C07C233/10
CPC分类号： C07C277/08 , C07C279/12
摘要： The present invention relates to a method for synthesis of an acrylamide derivative, starting with dissolving a salt of a nucleophilic amine in water to form an aqueous solution and desalting said solution with a base, comprising the following steps: a) addition of dissolved activated acrylic acid derivative to said solution; b) acidification of aqueous phase; and c) extraction of said aqueous phase.
摘要翻译： 本发明涉及一种合成丙烯酰胺衍生物的方法，首先将亲核胺的盐溶解在水中形成水溶液，并用碱脱盐所述溶液，包括以下步骤：a）加入溶解的活化丙烯酸 酸衍生物至所述溶液; b）水相酸化; 和c）提取所述水相。
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公开(公告)号：US09944722B2
公开(公告)日：2018-04-17
申请号：US13701074
申请日：2011-05-30
申请人： Mattias Algotsson , Lars C. Andersson , Eggert Brekkan , Lee Hedemyr , Jean-Luc Maloisel , Ronnie Palmgren
发明人： Mattias Algotsson , Lars C. Andersson , Eggert Brekkan , Lee Hedemyr , Jean-Luc Maloisel , Ronnie Palmgren
IPC分类号： C07C237/10 , C08B37/00 , B01J20/289 , B01J20/32 , C07C323/60 , B01J20/281
CPC分类号： C08B37/0039 , B01J20/281 , B01J20/289 , B01J20/3219 , B01J20/3251 , B01J20/3265 , C07C237/10 , C07C323/60
摘要： The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.The dimeric pentadentate chelator according to the invention has the following general formula: wherein Sc is a scaffold or a connecting structure that contains at least two functional groups enabling coupling of two pentadentate chelators (PD), and PD is a pentadentate chelator having the formula:
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公开(公告)号：US20130330751A1
公开(公告)日：2013-12-12
申请号：US13985360
申请日：2012-02-27
申请人： Philip Robert Mallender , Douglas Mark Green , Sarah Janet Clegg , Barry Edmund Johnson , Jimmy Hedin Dahlstrom , Mattias Algotsson , Ronnie Palmgren
发明人： Philip Robert Mallender , Douglas Mark Green , Sarah Janet Clegg , Barry Edmund Johnson , Jimmy Hedin Dahlstrom , Mattias Algotsson , Ronnie Palmgren
IPC分类号： G01N1/28
摘要： The invention discloses a sample preservation method which comprises the following steps: a) providing a paper substrate comprising ligands, where the ligands comprise charged groups, b) applying a sample comprising at least one analyte and at least one contaminant on the paper substrate, c) drying the sample on the paper substrate and, d) extracting at least part of the paper substrate to provide a solution of the analyte.
摘要翻译： 本发明公开了一种样品保存方法，其包括以下步骤：a）提供包含配体的纸基质，其中配体包含带电基团，b）在纸基材上施加包含至少一种分析物和至少一种污染物的样品，c ）干燥纸基底上的样品，以及d）提取至少部分纸基底以提供分析物的溶液。
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公开(公告)号：US20110039336A1
公开(公告)日：2011-02-17
申请号：US12989481
申请日：2009-04-24
申请人： Mattias Algotsson , Asa Bjurling
发明人： Mattias Algotsson , Asa Bjurling
IPC分类号： C12N5/00
CPC分类号： C12N5/0068 , C12N5/0075 , C12N2531/00 , C12N2533/50
摘要： The present invention relates to a product for cell culture. In particular, the invention relates to a cell growth surface having a coating comprising an animal protein free coating. The coating is derived from an enzymatic hydrolysate of a plant protein, preferably soy peptone. The cell culture product is preferably a microcarrier intended for culture of any anchorage-dependent cells.
摘要翻译： 本发明涉及一种用于细胞培养的产品。 特别地，本发明涉及具有包含无动物蛋白质涂层的涂层的细胞生长表面。 该涂层衍生自植物蛋白质的酶水解产物，优选大豆蛋白胨。 细胞培养产物优选是用于培养任何锚定依赖性细胞的微载体。
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公开(公告)号：US20080199959A1
公开(公告)日：2008-08-21
申请号：US11917169
申请日：2006-06-19
申请人： Mattias Algotsson , Gunnar Glad , Nicolas Thevenin
发明人： Mattias Algotsson , Gunnar Glad , Nicolas Thevenin
CPC分类号： C12N5/0075 , G01N33/5005 , G01N33/5432
摘要： The present invention relates to a method for cell culture, more precisely small scale cell culture. In the present invention a screening tool is used which comprises particulate matter or microcarriers, such as beads, attached to a solid support, such as a microtiter plate, for the cultivation of cells on said microcarriers. The microcarriers are preferably cultivation beads, such as CYTODEX™. According to the invention, this small scale format for cell cultivation may be used for any testing involving cells, for example testing of optimal growth conditions for a specific type of cell, such as stem cells. Another use is cell expansion.
摘要翻译： 本发明涉及一种细胞培养方法，更准确地说是小规模细胞培养。 在本发明中，使用了一种筛选工具，其包括连接到固体支持物例如微量滴定板上的颗粒物质或微载体，例如珠，用于在所述微载体上培养细胞。 微载体优选是培养珠，例如CYTODEX TM。 根据本发明，用于细胞培养的这种小规模格式可用于涉及细胞的任何测试，例如测试特定类型细胞如干细胞的最佳生长条件。 另一个用途是细胞扩增。
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