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    Process for the production of fermentation broth with increased vitamin B.sub.12 content by synchronizing the bacterium population
    2.
    发明授权
    Process for the production of fermentation broth with increased vitamin B.sub.12 content by synchronizing the bacterium population 失效
    生产具有增加维生素B的发酵肉汤的方法{HD 12 {通过同步细菌群体的B含量

    公开(公告)号：US3979259A

    公开(公告)日：1976-09-07

    申请号：US517788

    申请日：1974-10-24

    申请人： Bela Johan ,  Laszlo Szemler ,  Tamas Szontagh ,  Laszlo Kuti ,  Emilia Simonovits ,  Judit Bekes nee Erdos ,  Denes Szekely ,  Janos Kiss ,  Erzsebet Kovacs nee Komoroczy ,  Aniko Hargitai nee Franyo

    发明人： Bela Johan ,  Laszlo Szemler ,  Tamas Szontagh ,  Laszlo Kuti ,  Emilia Simonovits ,  Judit Bekes nee Erdos ,  Denes Szekely ,  Janos Kiss ,  Erzsebet Kovacs nee Komoroczy ,  Aniko Hargitai nee Franyo

    IPC分类号： C12N1/32 ,  C12P5/02 ,  C12P19/42 ,  C12R1/01 ,  C12D5/06

    CPC分类号： C12P5/023 ,  C12P19/42 ,  Y02E50/343 ,  Y10S435/801 ,  Y10S435/802 ,  Y10S435/813

    摘要： The invention relates to a process for the production of a fermentation broth with increased vitamin B.sub.12 content by a fermentation process carried out with a methane-producing mixed bacterium population under anaerobic septic conditions in the presence of known nutrient components and precursors. According to the invention, methanol, preferably in an amount of 0.5 v/v %, is added for some days, preferably for 3 days to the fermentation broth containing vitamin B.sub.12 used as the starting substance. Thereafter a minor part, preferably 10% of the fermentation broth, is removed and an equal volume of a nutrient broth containing the usual components in tenfold concentrations is added periodically in every 5th to 12th day, then the removal of fermentation broth is interrupted for 0 to 2 days, preferably for 1 day, and only 0.4 to 1.5 v/v% of methanol are added to the fermentation broth, and subsequently a minor part, preferably 10% of the fermentation broth, is removed daily and an equal volume of a nutrient broth containing the usual components in the usual concentrations is added together with 0.4 to 1.5 v/v % of methanol, depending on the biogas production of the fermentation broth. This series of operations is repeated periodically during the complete fermentation procedure taking into consideration the pH of the fermentation broth.

    摘要翻译： 本发明涉及通过在已知营养成分和前体的存在下在厌氧脓毒症条件下用产甲烷混合菌群进行的发酵方法来生产具有增加的维生素B12含量的发酵液的方法。 根据本发明，优选以0.5v / v％的量将甲醇加入含有用作起始物质的维生素B12的发酵液中几天，优选3天。 此后，除去少量的发酵肉汤，并且每5至12天定期添加等体积的含有十倍浓度的通常组分的营养肉汤，然后将发酵肉汤的去除中断0 至2天，优选1天，并且仅将0.4至1.5v / v％的甲醇加入到发酵液中，随后每天除去少部分，优选10％的发酵液，并加入等体积的 根据发酵液的生物气产量，含有通常浓度的通常组分的营养肉汤与0.4至1.5v / v％的甲醇一起加入。 考虑到发酵液的pH值，在整个发酵过程中定期重复这一系列操作。