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1.发明申请公开(公告)号：US20200174000A1
公开(公告)日：2020-06-04
申请号：US16700157
申请日：2019-12-02
IPC分类号： G01N33/574
摘要： Various aspects and embodiments disclosed herein relate generally to the modelling, treatment, reducing resistance to the treatment, prevention, and diagnosis of diseases/symptoms related to ductal carcinoma in situ (DCIS). Embodiments include methods of detecting and/or treating diseases/symptoms related to ductal carcinoma in situ (DCIS), comprising the steps of: providing a sample of blood, cells, or tissue from a person suspected of having ductal carcinoma in situ; and detecting one or more epithelial markers in the sample.
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公开(公告)号：US20120270752A1
公开(公告)日：2012-10-25
申请号：US13252069
申请日：2011-10-03
IPC分类号： G06F19/10 , C40B40/10 , G01N33/573
CPC分类号： G06K9/00147 , G01N33/5091 , G01N33/57434 , G01N2800/52 , G01N2800/56 , G01N2800/60 , G06K9/6231
摘要： Cell profile data is stored. The cell profile data comprises multiplexed biometric images capturing the expression of a plurality of biomarkers by at least one tissue sample from a patient with respect to at least one field of view. Individual cells are delineated and segmented into compartments. At least one cell feature is calculated based on the cell's expression of each of a plurality of biomarkers. A first moment is calculated for each cell feature. The moments are examined for an association with a diagnosis, a prognosis of a response to treatment of a condition or disease. Apparatus for performing the foregoing steps are disclosed.
摘要翻译： 存储单元格简档数据。 细胞分布数据包括相对于至少一个视场从患者至少一个组织样本捕获多个生物标志物的表达的多重生物统计图像。 单个细胞被描绘并分割成隔室。 基于多个生物标记中的每一个的细胞表达来计算至少一个细胞特征。 计算每个单元格特征的第一时刻。 检查时间与诊断的关联，对病症或疾病的治疗的反应的预后。 公开了用于执行上述步骤的装置。
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公开(公告)号：US20080118944A1
公开(公告)日：2008-05-22
申请号：US11864093
申请日：2007-09-28
申请人： Melinda Larsen , Anup Sood , Michael J. Gerdes , Michael Christopher Montalto , Zhengyu Pang , Fiona Ginty
发明人： Melinda Larsen , Anup Sood , Michael J. Gerdes , Michael Christopher Montalto , Zhengyu Pang , Fiona Ginty
CPC分类号： G01N33/53 , C12Q1/68 , G01N33/542 , G01N33/582 , Y10T436/143333
摘要： Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets adhered to a solid support, binding at least one fluorescent probe to one or more target present in the sample, and observing a signal from the fluorescent probe. The method further includes the steps of oxidizing the bound fluorescent probe with a solution containing an oxidizing agent that substantially inactivates the fluorescent probe, binding at least one fluorescent probe to one or more target present in the sample, and observing a signal from the fluorescent probe. The methods disclosed herein also provide for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.
摘要翻译： 提供了探测生物样品中多个靶标的方法。 所述方法包括以下步骤：提供包含粘附到固体支持物上的多个靶的生物样品，将至少一种荧光探针结合到样品中存在的一种或多种靶，并观察来自荧光探针的信号。 所述方法还包括以下步骤：用含有基本上灭活荧光探针的氧化剂的溶液氧化结合的荧光探针，将至少一种荧光探针结合到样品中存在的一种或多种靶，并观察来自荧光探针的信号 。 本文公开的方法还提供结合，观察和氧化的多次迭代，用于导出关于单个样本中的多个目标的信息。 还提供了相关的套件。
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公开(公告)号：US07741045B2
公开(公告)日：2010-06-22
申请号：US11864085
申请日：2007-09-28
申请人： Michael J. Gerdes , Anup Sood , Michael Christopher Montalto , Ali Can , Fiona Ginty , Maureen Ann Bresnahan , Robert John Filkins , Zhengyu Pang
发明人： Michael J. Gerdes , Anup Sood , Michael Christopher Montalto , Ali Can , Fiona Ginty , Maureen Ann Bresnahan , Robert John Filkins , Zhengyu Pang
CPC分类号： G01N33/582 , C12Q1/6816 , G01N33/542 , C12Q2545/101 , C12Q2527/125
摘要： Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one fluorescent probe to one or more target present in the sample, and binding at least at least one control probe to one or more target present in the sample. The methods include the steps of observing a signal from the fluorescent probe and a control signal from the control probe and applying to the sample a basic solution containing an oxidizing agent that selectively inactivates the fluorescent probe and not the control probe. The methods further include the steps of binding at least one fluorescent probe to one or more target present in the sample and observing a signal from the fluorescent probe. The methods disclosed herein also provide for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.
摘要翻译： 提供了探测生物样品中多个靶标的方法。 所述方法包括以下步骤：提供含有多个靶标的生物样品，将至少一种荧光探针结合至样品中存在的一种或多种靶标，以及将至少一种对照探针结合至样品中存在的一种或多种靶标。 所述方法包括以下步骤：观察来自荧光探针的信号和来自对照探针的控制信号，并向样品施加含有选择性灭活荧光探针而不是对照探针的氧化剂的碱性溶液。 所述方法还包括将至少一种荧光探针结合到样品中存在的一种或多种目标物并观察来自荧光探针的信号的步骤。 本文公开的方法还提供结合，观察和氧化的多次迭代，用于导出关于单个样本中的多个目标的信息。 还提供了相关的套件。
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公开(公告)号：US20090253163A1
公开(公告)日：2009-10-08
申请号：US12061044
申请日：2008-04-02
申请人： Jun Xie , Fiona Ginty , Robert John Filkins , Michael Christopher Montalto , Anup Sood , Jeffrey Bernard Fortin , Wei-Cheng Tian , Michael John Gerdes
发明人： Jun Xie , Fiona Ginty , Robert John Filkins , Michael Christopher Montalto , Anup Sood , Jeffrey Bernard Fortin , Wei-Cheng Tian , Michael John Gerdes
CPC分类号： G01N1/312
摘要： Automated methods and devices that facilitate iterative staining of biological samples from imaging applications are provided. The methods include the steps of providing a small volume flow cell containing a biological sample, applying a stain to the biological sample, combining at least two precursor reagents to form an activated destaining agent and wherein the activated destaining agent decomposition rate is greater than or similar to the destaining reaction rate, and flowing the destaining agent over the biological sample at a flow rate that is greater than the decomposition rate of the activated destaining agent. The process of staining, combining and flowing may be iteratively repeated. Also disclosed herein are devices for iterative staining of biological samples comprising a flow cell, in fluid communication with a premixer, wherein the volume capacity of the premixer is smaller than about five times the volume capacity of the flow cell.
摘要翻译： 提供了促进成像应用中生物样品的迭代染色的自动化方法和装置。 所述方法包括以下步骤：提供含有生物样品的小体积流动池，向生物样品施加染色，将至少两种前体试剂结合以形成活化的脱色剂，并且其中所述活化脱色剂分解速率大于或等于 与脱色反应速率相关联，并使脱色剂以大于活化脱色剂分解速率的流速流过生物样品。 染色，组合和流动的过程可以迭代地重复。 本文还公开了用于重复染色生物样品的装置，其包括与预混合器流体连通的流动池，其中预混合器的体积容量小于流动池的体积容量的约五倍。
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公开(公告)号：US20080118934A1
公开(公告)日：2008-05-22
申请号：US11864085
申请日：2007-09-28
申请人： Michael J. Gerdes , Anup Sood , Michael Christopher Montalto , Ali Can , Fiona Ginty , Maureen Ann Bresnahan , Robert John Filkins , Zhengyu Pang
发明人： Michael J. Gerdes , Anup Sood , Michael Christopher Montalto , Ali Can , Fiona Ginty , Maureen Ann Bresnahan , Robert John Filkins , Zhengyu Pang
IPC分类号： G01N33/53 , G01N33/566 , G01N33/567
CPC分类号： G01N33/582 , C12Q1/6816 , G01N33/542 , C12Q2545/101 , C12Q2527/125
摘要： Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one fluorescent probe to one or more target present in the sample, and binding at least at least one control probe to one or more target present in the sample. The methods include the steps of observing a signal from the fluorescent probe and a control signal from the control probe and applying to the sample a basic solution containing an oxidizing agent that selectively inactivates the fluorescent probe and not the control probe. The methods further include the steps of binding at least one fluorescent probe to one or more target present in the sample and observing a signal from the fluorescent probe. The methods disclosed herein also provide for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.
摘要翻译： 提供了探测生物样品中多个靶标的方法。 所述方法包括以下步骤：提供含有多个靶标的生物样品，将至少一种荧光探针结合至样品中存在的一种或多种靶标，以及将至少一种对照探针结合至样品中存在的一种或多种靶标。 所述方法包括以下步骤：观察来自荧光探针的信号和来自对照探针的控制信号，并向样品施加含有选择性灭活荧光探针而不是对照探针的氧化剂的碱性溶液。 所述方法还包括将至少一种荧光探针结合到样品中存在的一种或多种目标物并观察来自荧光探针的信号的步骤。 本文公开的方法还提供结合，观察和氧化的多次迭代，用于导出关于单个样本中的多个目标的信息。 还提供了相关的套件。
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公开(公告)号：US20080032321A1
公开(公告)日：2008-02-07
申请号：US11686649
申请日：2007-03-15
申请人： FIONA GINTY , ROBERT JOHN FILKINS , HARVEY ELLIS CLINE , MICHAEL CHRISTOPHER MONTALTO , SUDESHNA ADAK , ALI CAN , MICHAEL JOHN GERDES , MELINDA LARSEN
发明人： FIONA GINTY , ROBERT JOHN FILKINS , HARVEY ELLIS CLINE , MICHAEL CHRISTOPHER MONTALTO , SUDESHNA ADAK , ALI CAN , MICHAEL JOHN GERDES , MELINDA LARSEN
IPC分类号： G01N33/53
CPC分类号： C12Q1/485 , G06K9/00147 , G06T7/11 , G06T7/143 , G06T2207/10056 , G06T2207/30024
摘要： A system for analyzing tissue samples, comprising: a storage device for at least temporarily storing one or more images of one or more cells, wherein at least one of the images is indicative of one or more channels comprising a receptor tyrosine kinase (RTK); and a processing device that determines an extent to which one or more of the RTKs may have translocated from at least one subcellular region to another subcellular region of one or more of the cells; and generates a score based at least in part on the RTK translocation.
摘要翻译： 一种用于分析组织样本的系统，包括：用于至少临时存储一个或多个细胞的一个或多个图像的存储装置，其中所述图像中的至少一个指示包含受体酪氨酸激酶（RTK）的一个或多个通道; 以及处理设备，其确定一个或多个RTK可以从一个或多个单元的至少一个亚细胞区域移位到另一个亚细胞区域的程度; 并且至少部分地基于RTK易位产生得分。
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公开(公告)号：US20120269418A1
公开(公告)日：2012-10-25
申请号：US13252072
申请日：2011-10-03
IPC分类号： G06K9/00 , G01N33/577
CPC分类号： G06K9/00147 , G01N33/5091 , G01N33/57434 , G01N2800/52 , G01N2800/56 , G01N2800/60 , G06K9/6231
摘要： A data set of cell profile data is stored. The cell profile data includes multiplexed biometric image data describing the expression of a plurality of biomarkers with respect to a field of view in which individual cells are delineated and segmenting into compartments. Each cell in the field is assigned to a single cluster in a selected set of clusters based on cell similarity; cell similarity is determined based on a plurality of selected cell attributes. The proportion of cells in each cluster is observed. The observed proportions are examined for an association with a diagnosis, a prognosis, or a response derived from a known association of the selected set of clusters with at least one piece of meta-information including a field of view level assessment or a patient-level assessment.
摘要翻译： 存储小区简档数据的数据集。 细胞谱图数据包括描述多个生物标志物相对于其中单个细胞被描绘和分割成隔室的视野的表达的多重生物统计图像数据。 基于小区相似性，将该字段中的每个小区分配给所选集群中的单个群集; 基于多个选择的小区属性来确定小区相似性。 观察每个簇中细胞的比例。 检查观察到的比例与来自所选择的集合集合的已知关联的诊断，预后或响应的关联与至少一个元信息（包括视野级别评估或患者级别） 评定。
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公开(公告)号：US07741046B2
公开(公告)日：2010-06-22
申请号：US11864093
申请日：2007-09-28
申请人： Melinda Larsen , Anup Sood , Michael J. Gerdes , Michael Christopher Montalto , Zhengyu Pang , Fiona Ginty
发明人： Melinda Larsen , Anup Sood , Michael J. Gerdes , Michael Christopher Montalto , Zhengyu Pang , Fiona Ginty
CPC分类号： G01N33/53 , C12Q1/68 , G01N33/542 , G01N33/582 , Y10T436/143333
摘要： Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets adhered to a solid support, binding at least one fluorescent probe to one or more target present in the sample, and observing a signal from the fluorescent probe. The method further includes the steps of oxidizing the bound fluorescent probe with a solution containing an oxidizing agent that substantially inactivates the fluorescent probe, binding at least one fluorescent probe to one or more target present in the sample, and observing a signal from the fluorescent probe. The methods disclosed herein also provide for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.
摘要翻译： 提供了探测生物样品中多个靶标的方法。 所述方法包括以下步骤：提供包含粘附到固体支持物上的多个靶的生物样品，将至少一种荧光探针结合到样品中存在的一种或多种靶，并观察来自荧光探针的信号。 所述方法还包括以下步骤：用含有基本上灭活荧光探针的氧化剂的溶液氧化结合的荧光探针，将至少一种荧光探针结合到样品中存在的一种或多种靶，并观察来自荧光探针的信号 。 本文公开的方法还提供结合，观察和氧化的多次迭代，用于导出关于单个样本中的多个目标的信息。 还提供了相关的套件。
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