公开(公告)号：US06297391B1

公开(公告)日：2001-10-02

申请号：US09366744

申请日：1999-08-04

Applicant: Giampietro Borsotti ,  Ezio Battistel ,  Francesco Cellini ,  Rina Iannacone

Inventor： Giampietro Borsotti ,  Ezio Battistel ,  Francesco Cellini ,  Rina Iannacone

IPC: C07C5300

CPC classification number: C12P7/6481 ,  C07F9/10 ,  Y02P20/55

Abstract: Diricinoleylphosphatidylcholine (I) in an optically active form is obtained with good yields and a high purity by means of a process which comprises: a) esterifying the terminal carboxylic group of ricinoleic acid (III) with an alcohol having from 1 to 4 carbon atoms to give the corresponding ester having formula (IV); b) protecting the hydroxylic group of the ester of ricinoleic acid (IV) with a protecting group removable under bland operating conditions and isolating the ester of ricinoleic acid of which the hydroxylic group (V) is protected; c) hydrolyzing the ester of ricinoleic acid of which the hydroxylic group (V) is protected and isolating the ricinoleic acid of which the hydroxylic group (VI) is protected; d) acylating L-&agr;-glycerophosphatidylcholine (II) with an imidazolic or triazolic derivative of the compound having formula (VI) and isolating the diricinoleylphosphatidylcholine of which the hydroxylic group (VII) is protected; e) removing the protecting group from the hydroxylic group of the compound having formula (VII); and finally f) recovering and purifying the compound having formula (I) by means of chromatography.

Abstract translation: 通过以下方法获得具有光学活性形式的二亚油酰基磷脂酰胆碱（I），具有良好的收率和高纯度，该方法包括：a）用具有1至4个碳原子的醇将蓖麻油酸（III）的末端羧基酯化至 得到具有式（IV）的相应酯; b）用温和的操作条件下可除去的保护基保护蓖麻油酸（IV）的酯的羟基，并将其中羟基（Ⅴ）的蓖麻油酸酯 保护; c）水解羟基（V）被保护的蓖麻油酸酯，并分离保护羟基（Ⅵ）的蓖麻油酸; d）用咪唑单体酰化L-α-甘油磷脂酰胆碱（II） 或具有式（Ⅵ）的化合物的三唑衍生物，并分离保护羟基（Ⅶ）的二亚油酰基磷脂酰胆碱; e）从羟基中除去保护基 具有式（VII）的化合物的式 最后通过色谱法回收和纯化具有式（I）的化合物。

公开(公告)号：US20070009891A1

公开(公告)日：2007-01-11

申请号：US10561809

申请日：2003-06-26

Applicant: Francesco Cellini ,  Rosa Cifarelli ,  Maria Gallitelli ,  Teresa Mango ,  Giuseppe Lauria ,  Lucia Semeraro

Inventor： Francesco Cellini ,  Rosa Cifarelli ,  Maria Gallitelli ,  Teresa Mango ,  Giuseppe Lauria ,  Lucia Semeraro

IPC: C40B30/06 ,  C40B40/08 ,  C12Q1/68

CPC classification number: C12N15/1065 ,  C12N15/1072

Abstract: A method is described for the isolation of Expressed Sequence Tags (ESTs) which are based on the use of suitable quantities of 5-azaCytidine in the germination phase of plants to induce the demethylation phenomenon of the DNA. In this way, it is possible to obtain “totipotent” seedlings, i.e. capable of expressing as many genes as possible regardless of the development phase of the plant and isolating said genes by the synthesis of a single cDNA library.

Abstract translation: 描述了分离表达序列标签（ESTs）的方法，其基于在植物发芽期中使用合适数量的5-氮杂胞苷诱导DNA脱甲基现象。 以这种方式，可以获得“全能”幼苗，即能够表达尽可能多的基因，而不管植物的发育阶段如何，并通过合成单个cDNA文库来分离所述基因。

公开(公告)号：US4789741A

公开(公告)日：1988-12-06

申请号：US165234

申请日：1988-03-07

Applicant: Francesco Cellini ,  Vittorio Gervasutti ,  Raffaele Tancorra ,  Sergio Tonti

Inventor： Francesco Cellini ,  Vittorio Gervasutti ,  Raffaele Tancorra ,  Sergio Tonti

IPC: B01J19/26 ,  C07D201/04

CPC classification number: C07D201/04 ,  B01J19/26 ,  B01J2219/00119

Abstract: The invention concerns a two-step process for the synthesis of caprolactam by reaction of cylcohexanone-oxime with an excess of oleum, carried out in a first step with only a portion of the oxime and in the presence of liquid SO.sub.2, according to usual "cold" techniques, the amount of free SO.sub.3 in the oleum being equal to or greater than 50% by weight, wherein said "cold" step is completed by a second "hot" step, by adding a second portion of oxime, the ratio between said second portion and said first portion ranging from 0.5 to 1.2.

公开(公告)号：US20100003223A1

公开(公告)日：2010-01-07

申请号：US12310236

申请日：2007-07-26

Applicant: Rosa Anna Cifarelli ,  Francesco Cellini ,  Rosa Di Liddo ,  Pier Paolo Parnigotto

Inventor： Rosa Anna Cifarelli ,  Francesco Cellini ,  Rosa Di Liddo ,  Pier Paolo Parnigotto

IPC: A61K35/12 ,  C12N5/08 ,  A61P43/00

CPC classification number: C12N5/0653 ,  C12N2501/06 ,  C12N2506/00

Abstract: The invention relates to a method for the production of multipotent stem cells starting from highly differentiated adult somatic cells of mammals or their precursors comprising the demethylating treatment phase of highly differentiated cells with 5′ Aza 2′ cytidine and relative kits and uses in the medical field.

Abstract translation: 本发明涉及一种用于从高分化的哺乳动物成体体细胞或其前体产生多能干细胞的方法，其包括具有5'氮杂2'胞苷的高度分化细胞的去甲基化处理阶段以及在医学领域中的相关试剂盒和用途 。

公开(公告)号：US5959181A

公开(公告)日：1999-09-28

申请号：US854170

申请日：1997-05-09

Applicant: Francesco Cellini ,  Pasquale Domenico Grieco

Inventor： Francesco Cellini ,  Pasquale Domenico Grieco

IPC: C12N15/05 ,  C12N15/40 ,  C12N15/82 ,  A01H1/00 ,  C12N15/33 ,  C12N15/64

CPC classification number: C12N15/8283

Abstract: The present invention relates to a method of preparation of transgenic plants resistant to viral infections by introducing into the genoma of a host plant an antisense gene construct constituted by: the domain F of the subgenomic promoter of a viral RNA; a leader sequence of a viral ORF, downstream from said subgenomic promoter; the gene encoding a viral coat protein, downstream from said leader sequence; and the 3'-terminal region of a viral RNA, downstream from said gene. The present invention also relates to a recombinant vector comprising a promoter functional in a host plant, and, operably linked to this promoter, the antisense gene construct of the present invention.

Abstract translation: 本发明涉及通过向宿主植物的基因体中引入由以下构成的反义基因构建体来制备对病毒感染有抗性的转基因植物的方法：由病毒RNA的亚基因组启动子的结构域F; 来自所述亚基因组启动子的病毒ORF的前导序列; 编码病毒外壳蛋白的基因，在所述前导序列的下游; 和病毒RNA的3'末端区域。 本发明还涉及包含在宿主植物中起作用的启动子，并且与该启动子可操作地连接的本发明的反义基因构建体的重组载体。