公开(公告)号：US06180363B2

公开(公告)日：2001-01-30

申请号：US09158767

申请日：1998-09-23

Applicant: Yannick Batard ,  Francis Durst ,  Michel Schalk ,  Daniele Werck-Reichhart

Inventor： Yannick Batard ,  Francis Durst ,  Michel Schalk ,  Daniele Werck-Reichhart

IPC: C12P2102

CPC classification number: C12N9/0042 ,  C12N15/67 ,  C12N15/81

Abstract: The present invention relates to a DNA sequence which encodes a protein of interest which contains regions having a high content of codons which are poorly suited to yeasts, characterized in that a sufficient number of codons which are poorly suited to yeasts is replaced with corresponding codons which are well-suited to yeasts in the said regions having a high content of codons which are poorly suited to yeasts. The present invention relates, more specifically, to DNA sequences which originate from dicotyledonous or monocotyledonous plants, in particular plants of the graminae family which are selected, in particular, from among wheat, barley, oats, rice, maize, sorghum and cane sugar. The present invention also relates to transformed yeasts which contain a DNA sequence according to the invention.

Abstract translation: 本发明涉及编码感兴趣的蛋白质的DNA序列，该DNA序列含有密度较高且不适于酵母的密度较高的区域，其特征在于足够数量的不适于酵母的密码子被相应的密码子替换， 在不适合酵母的密码子含量较高的所述区域中适用于酵母菌。 本发明更具体地涉及源于双子叶植物或单子叶植物的DNA序列，特别是特别是选自小麦，大麦，燕麦，稻，玉米，高粱和蔗糖中的格氏族的植物。 本发明还涉及含有根据本发明的DNA序列的转化酵母。

公开(公告)号：US06787337B1

公开(公告)日：2004-09-07

申请号：US09713794

申请日：2000-11-15

Applicant: Yannick Batard ,  Francis Durst ,  Michel Schalk ,  Daniele Werck-Reichhart

Inventor： Yannick Batard ,  Francis Durst ,  Michel Schalk ,  Daniele Werck-Reichhart

IPC: C12P2100

CPC classification number: C12N9/0042 ,  C12N15/67 ,  C12N15/81

Abstract: The present invention relates to a recombinant non-yeast DNA, which encodes a protein of interest, wherein an unmodified DNA corresponding to the recombinant non-yeast DNA contains a region having a high content of codons that are poorly suited to yeasts, wherein a number of the codons that are poorly suited to yeasts are replaced in said region of the recombinant non-yeast DNA with synonymous codons coding for the same amino acid that are well-suited to yeasts, and wherein the number of replaced codons is sufficient to permit expression in yeasts. The present invention also relates to DNA sequences which originate from dicotyledonous or monocotyledonous plants, and in particular plants of the graminae family which are selected from among wheat, barley, oats, rice, maize, sorghum and cane sugar, as well as to vectors and transformed yeasts which contain the DNA sequences of the invention.

Abstract translation: 本发明涉及编码感兴趣的蛋白质的重组非酵母DNA，其中对应于重组非酵母DNA的未修饰的DNA含有具有高度不适于酵母的密码子的高含量区域，其中数目 的不适于酵母的密码子在所述重组非酵母DNA的所述区域中被替换，其具有编码相当于酵母的相同氨基酸的同义密码子，并且其中替换的密码子的数目足以允许表达 在酵母中 本发明还涉及源自双子叶植物或单子叶植物的DNA序列，特别是选自小麦，大麦，燕麦，稻，玉米，高粱和蔗糖的格氏族的植物，以及载体和 转化酵母含有本发明的DNA序列。

公开(公告)号：US06376753B1

公开(公告)日：2002-04-23

申请号：US09126420

申请日：1998-07-30

Applicant: Yannick Batard ,  Tiburce Robineau ,  Francis Durst ,  Daniele Werck-Reichhart ,  Luc Didierjean

Inventor： Yannick Batard ,  Tiburce Robineau ,  Francis Durst ,  Daniele Werck-Reichhart ,  Luc Didierjean

IPC: C12N1582

CPC classification number: C12N9/0071 ,  C12N9/0077 ,  C12N15/8259 ,  C12N15/8274

Abstract: The present invention relates to the gene CYP76B1 of a cytochrome P450 which has been isolated from Helianthus tuberosus tuber. The expression of this gene in yeast has shown that it encoded an enzyme very actively catalysing the O-dealkylation of various exogenous molecules with a high efficiency, such as phenylureas. The expression of CYP76B1 is strongly induced in plants brought into contact with certain exogenous metals or organic compounds. This induction may be exploited for the detection of environmental pollutants. CYP76B1 can be also used to alter the resistance of plants sensitive to this family of herbicides and for soil and groundwater bioremedation.

Abstract translation: 本发明涉及从向日葵中分离的细胞色素P450的基因CYP76B1。 酵母中该基因的表达表明，它非常有效地催化了高效催化各种外源性分子的O-脱烷基化酶，如苯脲。 在与某些外源金属或有机化合物接触的植物中，CYP76B1的表达被强烈诱导。 该诱导可用于检测环境污染物。 CYP76B1也可用于改变对这种除草剂家族和土壤和地下水生物修复敏感的植物的抗性。