公开(公告)号：US5916743A

公开(公告)日：1999-06-29

申请号：US467818

申请日：1995-06-06

申请人： William C. Lake ,  Richard Giesler ,  Dennis Van Epps ,  John R. Chapman ,  Jeffrey A. Martinson ,  Dale R. Ellis ,  Frederick Aono ,  Daniel F. Bischof

发明人： William C. Lake ,  Richard Giesler ,  Dennis Van Epps ,  John R. Chapman ,  Jeffrey A. Martinson ,  Dale R. Ellis ,  Frederick Aono ,  Daniel F. Bischof

IPC分类号： A61M1/36 ,  C12N5/00 ,  C12N5/02 ,  C12N5/06 ,  C12Q1/24 ,  G01N33/50 ,  G01N33/569 ,  A01N1/02 ,  C12N1/02 ,  C12Q1/68

CPC分类号： G01N33/56972 ,  A61M1/362 ,  A61M1/3696 ,  C12Q1/24 ,  G01N33/5002 ,  G01N33/5005 ,  G01N33/569 ,  Y10T436/111666 ,  Y10T436/25375 ,  Y10T436/2575

摘要： A method combining the techniques of immunoaffinity separation and continuous flow centrifugal separation is provided for selective separation of a nucleated heterogeneous cell population from a heterogeneous cell mixture. The heterogeneous cell mixture is intimately contacted to promote binding thereto by particles having attached a substance that actively binds to a specific desired type of cell out of the cell mixture. The particles are selected so that the sedimentation velocity of the particle/cell conjugate differs sufficiently from those of other cells in the cell mixture to allow its separation by means of a continuous flow cell separator. The method rapidly processes large volumes of cell mixture with the high accuracy expected of immunoaffinity separation and can be used to separate, for example, various types of leukocytes from whole blood, bone marrow concentrate, or a peripheral blood stem cell concentrate; or precursors of lymphokine activated killer cells, tumor infiltrating lymphocyte cells, or activated killer monocytes from lymphocyte or monocyte cell concentrates or from a tissue cell preparation.

摘要翻译： 提供了结合免疫亲和分离和连续流离心分离技术的方法，用于从异质细胞混合物中选择性分离成核的异质细胞群。 非均相细胞混合物紧密接触以促进与附着有活性结合特定所需类型的细胞的细胞混合物的颗粒结合。 选择颗粒使得颗粒/细胞共轭体的沉降速度与细胞混合物中的其它细胞的沉降速度充分不同，以允许其通过连续流动细胞分离器分离。 该方法以预期的免疫亲和力分离的高精度快速处理大量的细胞混合物，并且可用于分离例如来自全血，骨髓浓缩物或外周血干细胞浓缩物的各种类型的白细胞; 或淋巴因子活化的杀伤细胞的前体，肿瘤浸润性淋巴细胞细胞或来自淋巴细胞或单核细胞浓缩物或来自组织细胞制剂的活化的杀伤单核细胞。

公开(公告)号：US5641622A

公开(公告)日：1997-06-24

申请号：US255107

申请日：1994-06-07

申请人： William C. Lake ,  Richard Giesler ,  Dennis Van Epps ,  John R. Chapman ,  Jeffrey A. Martinson ,  Dale R. Ellis ,  Frederick Aono ,  Daniel F. Bischof

发明人： William C. Lake ,  Richard Giesler ,  Dennis Van Epps ,  John R. Chapman ,  Jeffrey A. Martinson ,  Dale R. Ellis ,  Frederick Aono ,  Daniel F. Bischof

IPC分类号： A61M1/36 ,  C12N5/00 ,  C12N5/02 ,  C12N5/06 ,  C12Q1/24 ,  G01N33/50 ,  G01N33/569 ,  A01N1/02 ,  C12N1/02 ,  C12Q1/68

CPC分类号： G01N33/56972 ,  A61M1/362 ,  A61M1/3696 ,  C12Q1/24 ,  G01N33/5002 ,  G01N33/5005 ,  G01N33/569 ,  Y10T436/111666 ,  Y10T436/25375 ,  Y10T436/2575

摘要： A method combining the techniques of immunoaffinity separation and continuous flow centrifugal separation is provided for selective separation of a nucleated heterogeneous cell population from a heterogeneous cell mixture. The heterogeneous cell mixture is intimately contacted to promote binding thereto by particles having attached a substance that actively binds to a specific desired type of cell out of the cell mixture. The particles are selected so that the sedimentation velocity of the particle/cell conjugate differs sufficiently from those of other cells in the cell mixture to allow its separation by means of a continuous flow cell separator. The method rapidly processes large volumes of cell mixture with the high accuracy expected of immunoaffinity separation and can be used to separate, for example, various types of leukocytes from whole blood, bone marrow concentrate, or a peripheral blood stem cell concentrate; or precursors of lymphokine activated killer cells, tumor infiltrating lymphocyte cells, or activated killer monocytes from lymphocyte or monocyte cell concentrates or from a tissue cell preparation.

摘要翻译： 提供了结合免疫亲和分离和连续流离心分离技术的方法，用于从异质细胞混合物中选择性分离成核的异质细胞群。 非均相细胞混合物紧密接触以促进与附着有活性结合特定所需类型的细胞的细胞混合物的颗粒结合。 选择颗粒使得颗粒/细胞共轭体的沉降速度与细胞混合物中的其它细胞的沉降速度充分不同，以允许其通过连续流动细胞分离器分离。 该方法以预期的免疫亲和力分离的高精度快速处理大量的细胞混合物，并且可用于分离例如来自全血，骨髓浓缩物或外周血干细胞浓缩物的各种类型的白细胞; 或淋巴因子活化的杀伤细胞的前体，肿瘤浸润性淋巴细胞细胞或来自淋巴细胞或单核细胞浓缩物或来自组织细胞制剂的活化的杀伤单核细胞。