公开(公告)号：US5641622A

公开(公告)日：1997-06-24

申请号：US255107

申请日：1994-06-07

申请人： William C. Lake ,  Richard Giesler ,  Dennis Van Epps ,  John R. Chapman ,  Jeffrey A. Martinson ,  Dale R. Ellis ,  Frederick Aono ,  Daniel F. Bischof

发明人： William C. Lake ,  Richard Giesler ,  Dennis Van Epps ,  John R. Chapman ,  Jeffrey A. Martinson ,  Dale R. Ellis ,  Frederick Aono ,  Daniel F. Bischof

IPC分类号： A61M1/36 ,  C12N5/00 ,  C12N5/02 ,  C12N5/06 ,  C12Q1/24 ,  G01N33/50 ,  G01N33/569 ,  A01N1/02 ,  C12N1/02 ,  C12Q1/68

CPC分类号： G01N33/56972 ,  A61M1/362 ,  A61M1/3696 ,  C12Q1/24 ,  G01N33/5002 ,  G01N33/5005 ,  G01N33/569 ,  Y10T436/111666 ,  Y10T436/25375 ,  Y10T436/2575

摘要： A method combining the techniques of immunoaffinity separation and continuous flow centrifugal separation is provided for selective separation of a nucleated heterogeneous cell population from a heterogeneous cell mixture. The heterogeneous cell mixture is intimately contacted to promote binding thereto by particles having attached a substance that actively binds to a specific desired type of cell out of the cell mixture. The particles are selected so that the sedimentation velocity of the particle/cell conjugate differs sufficiently from those of other cells in the cell mixture to allow its separation by means of a continuous flow cell separator. The method rapidly processes large volumes of cell mixture with the high accuracy expected of immunoaffinity separation and can be used to separate, for example, various types of leukocytes from whole blood, bone marrow concentrate, or a peripheral blood stem cell concentrate; or precursors of lymphokine activated killer cells, tumor infiltrating lymphocyte cells, or activated killer monocytes from lymphocyte or monocyte cell concentrates or from a tissue cell preparation.

摘要翻译： 提供了结合免疫亲和分离和连续流离心分离技术的方法，用于从异质细胞混合物中选择性分离成核的异质细胞群。 非均相细胞混合物紧密接触以促进与附着有活性结合特定所需类型的细胞的细胞混合物的颗粒结合。 选择颗粒使得颗粒/细胞共轭体的沉降速度与细胞混合物中的其它细胞的沉降速度充分不同，以允许其通过连续流动细胞分离器分离。 该方法以预期的免疫亲和力分离的高精度快速处理大量的细胞混合物，并且可用于分离例如来自全血，骨髓浓缩物或外周血干细胞浓缩物的各种类型的白细胞; 或淋巴因子活化的杀伤细胞的前体，肿瘤浸润性淋巴细胞细胞或来自淋巴细胞或单核细胞浓缩物或来自组织细胞制剂的活化的杀伤单核细胞。

公开(公告)号：US5336760A

公开(公告)日：1994-08-09

申请号：US838711

申请日：1992-03-12

申请人： R. Alan Hardwick ,  Alan K. Smith ,  William C. Lake ,  Dennis E. Chenoweth

发明人： R. Alan Hardwick ,  Alan K. Smith ,  William C. Lake ,  Dennis E. Chenoweth

IPC分类号： A61K39/395 ,  A61K47/48 ,  B03C1/00 ,  B03C1/28 ,  C07K1/14 ,  C07K1/22 ,  C07K14/745 ,  C07K14/755 ,  C07K16/00 ,  B03C1/32 ,  C07K3/18 ,  C07K17/14 ,  G01N33/553

CPC分类号： A61K47/48369 ,  B03C1/288 ,  Y10S530/811

摘要： The invention provides an apparatus and method for the preparation of a pharmaceutical composition comprising a complex of an antigen component and the corresponding antibody component in a pharmacologically acceptable carrier. The apparatus comprises a housing chamber for containing paramagnetic particles and a platform against which the housing chamber is releasably retained. The platform contains one or more magnets located adjacent to the housing chamber when the housing chamber is positioned on the platform. The platform is secured to a support which allows the platform, and thus the housing chamber, to be rotated between a vertical and a horizontal position. The method involves supplying a quantity of paramagnetic particles bearing the antigen component to the housing chamber, introducing into the housing chamber a physiological fluid which contains the antibody component, mixing the paramagnetic particles and the physiological fluid to form an antigen component and antibody component complex on the paramagnetic beads, removing the physiological fluid from the housing chamber, and eluting the antigen component and antibody component complex from the paramagnetic beads in a pharmaceutically acceptable eluting solution.

摘要翻译： PCT No.PCT / US90 / 05228 Sec。 371日期：1992年3月12日 102（e）1992年3月12日PCT日期1990年9月14日PCT。本发明提供了制备药物组合物的装置和方法，该药物组合物在药理学上可接受的载体中包含抗原成分和相应的抗体成分的复合物。 该装置包括用于容纳顺磁性颗粒的容纳室和可释放地保持壳体室的平台。 当容纳室位于平台上时，该平台包含一个或多个邻近容纳室定位的磁体。 平台被固定到支撑件，该支撑件允许平台，并且因此容纳室在垂直位置和水平位置之间旋转。 该方法包括将一定数量的带有抗原成分的顺磁性颗粒供给到壳体室，将容纳抗体成分的生理液体引入壳体室内，混合顺磁颗粒和生理液体以形成抗原成分和抗体组分复合物 顺磁珠，从壳体室除去生理液体，并在药学上可接受的洗脱溶液中从顺磁珠洗脱抗原成分和抗体组分复合物。

公开(公告)号：US5916743A

公开(公告)日：1999-06-29

申请号：US467818

申请日：1995-06-06

申请人： William C. Lake ,  Richard Giesler ,  Dennis Van Epps ,  John R. Chapman ,  Jeffrey A. Martinson ,  Dale R. Ellis ,  Frederick Aono ,  Daniel F. Bischof

发明人： William C. Lake ,  Richard Giesler ,  Dennis Van Epps ,  John R. Chapman ,  Jeffrey A. Martinson ,  Dale R. Ellis ,  Frederick Aono ,  Daniel F. Bischof

IPC分类号： A61M1/36 ,  C12N5/00 ,  C12N5/02 ,  C12N5/06 ,  C12Q1/24 ,  G01N33/50 ,  G01N33/569 ,  A01N1/02 ,  C12N1/02 ,  C12Q1/68

CPC分类号： G01N33/56972 ,  A61M1/362 ,  A61M1/3696 ,  C12Q1/24 ,  G01N33/5002 ,  G01N33/5005 ,  G01N33/569 ,  Y10T436/111666 ,  Y10T436/25375 ,  Y10T436/2575

摘要： A method combining the techniques of immunoaffinity separation and continuous flow centrifugal separation is provided for selective separation of a nucleated heterogeneous cell population from a heterogeneous cell mixture. The heterogeneous cell mixture is intimately contacted to promote binding thereto by particles having attached a substance that actively binds to a specific desired type of cell out of the cell mixture. The particles are selected so that the sedimentation velocity of the particle/cell conjugate differs sufficiently from those of other cells in the cell mixture to allow its separation by means of a continuous flow cell separator. The method rapidly processes large volumes of cell mixture with the high accuracy expected of immunoaffinity separation and can be used to separate, for example, various types of leukocytes from whole blood, bone marrow concentrate, or a peripheral blood stem cell concentrate; or precursors of lymphokine activated killer cells, tumor infiltrating lymphocyte cells, or activated killer monocytes from lymphocyte or monocyte cell concentrates or from a tissue cell preparation.

摘要翻译： 提供了结合免疫亲和分离和连续流离心分离技术的方法，用于从异质细胞混合物中选择性分离成核的异质细胞群。 非均相细胞混合物紧密接触以促进与附着有活性结合特定所需类型的细胞的细胞混合物的颗粒结合。 选择颗粒使得颗粒/细胞共轭体的沉降速度与细胞混合物中的其它细胞的沉降速度充分不同，以允许其通过连续流动细胞分离器分离。 该方法以预期的免疫亲和力分离的高精度快速处理大量的细胞混合物，并且可用于分离例如来自全血，骨髓浓缩物或外周血干细胞浓缩物的各种类型的白细胞; 或淋巴因子活化的杀伤细胞的前体，肿瘤浸润性淋巴细胞细胞或来自淋巴细胞或单核细胞浓缩物或来自组织细胞制剂的活化的杀伤单核细胞。