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    Apparatus for separation of biologic components from heterogeneous cell populations
    4.
    发明授权
    Apparatus for separation of biologic components from heterogeneous cell populations 失效
    用于分离生物成分与异种细胞群体的装置

    公开(公告)号：US06221315B1

    公开(公告)日：2001-04-24

    申请号：US09290653

    申请日：1999-04-12

    申请人： Richard Giesler ,  John R. Chapman ,  Dale R. Ellis ,  Daniel F. Bischof

    发明人： Richard Giesler ,  John R. Chapman ,  Dale R. Ellis ,  Daniel F. Bischof

    IPC分类号： B01L1100

    CPC分类号： G01N33/56972 ,  A61M1/3618 ,  A61M1/362 ,  A61M1/3696 ,  C12Q1/24 ,  G01N33/5002 ,  G01N33/5005 ,  G01N33/569 ,  Y10T436/111666 ,  Y10T436/25375 ,  Y10T436/2575

    摘要： Apparatus for separating a biological component from a biological fluid are disclosed. The apparatus includes a chamber for receiving a biological fluid including a biological component, and a rotor for creating a centrifugal field within the chamber. A source of particles may be included wherein the particles have a binding site for selectively binding to the biological component. The particles may be aseptically introduced into the chamber. The apparatus may withdraw whole blood from a donor, separate the whole blood to obtain a target cell population and return a selected one of either the target cell population or at least a portion of the whole blood remainder to the donor or patient. A disposable separation inset for use in conjunction with a rotatable separation device such as the apparatus described above may also be provided.

    摘要翻译： 公开了用于从生物流体分离生物组分的装置。 该装置包括用于接收包括生物组分的生物流体的腔室和用于在腔室内产生离心场的转子。 可以包括颗粒源，其中颗粒具有用于选择性结合生物组分的结合位点。 颗粒可以无菌地引入室中。 该装置可以从供体抽出全血，分离全血以获得靶细胞群并将目标细胞群体中的所选择的一个或全部血液剩余物的至少一部分返回给供体或患者。 还可以提供与诸如上述装置的可旋转分离装置结合使用的一次性分离插入件。

    Centrifuge separation method and apparatus using a medium density fluid
    5.
    发明申请
    Centrifuge separation method and apparatus using a medium density fluid 有权

    公开(公告)号：US20130045852A1

    公开(公告)日：2013-02-21

    申请号：US13199129

    申请日：2011-08-19

    申请人： John R. Chapman ,  Rodney Sparks

    发明人： John R. Chapman ,  Rodney Sparks

    IPC分类号： B04B7/16 ,  B04B15/00 ,  B01D21/26

    CPC分类号： B01D21/26 ,  A61M1/3693 ,  B01D21/262 ,  B01D2221/10 ,  B01L3/5021 ,  B01L2200/026 ,  B01L2300/0864 ,  G01N33/491

    摘要： The centrifugation vessel includes an outer wall containing an interior space. A dam defines a barrier which divides the interior space into at least two regions including a catch basin defining a higher gee region and a reservoir defining a lower gee region. These regions are joined together over the dam. The dam includes a face which is preferably tapered to enable optimization of speed of separation of a sample placed within the vessel. The vessel is usable in a biological sample processing method by having the higher gee region of the vessel configured to have an elongate form and the volume optimized for collection of a higher density fraction of the sample. Supply and withdrawal tubes extend into the regions for reliable extraction and separate collection of differing density fractions after separation by centrifugation. A medium density fluid is used within the vessel to further isolate constituents of differing densities.

    Method of Preconditioning of Cell Suspensions
    9.
    发明申请
    Method of Preconditioning of Cell Suspensions 审中-公开
    细胞悬浮液预处理方法

    公开(公告)号：US20120213754A1

    公开(公告)日：2012-08-23

    申请号：US13398739

    申请日：2012-02-16

    申请人： John R. Chapman

    发明人： John R. Chapman

    IPC分类号： A61K35/14 ,  A61K35/18 ,  A61K35/12 ,  A61K35/16 ,  C12N5/071 ,  C12N5/0775

    CPC分类号： C12N5/0653 ,  A61J1/10 ,  A61K35/12 ,  A61K35/14 ,  A61K35/15 ,  A61K35/16 ,  A61K35/18 ,  A61K35/19 ,  B65D81/266 ,  C12N5/0647 ,  C12N5/0663 ,  C12N5/0665 ,  C12N5/0667 ,  C12N2500/02

    摘要： An apparatus for the preparation of cells for implantation into a living body is disclosed. The apparatus comprises a vessel substantially impermeable to gaseous oxygen; and fluid within said vessel, the fluid having a maximal dissolved oxygen capacity substantially equivalent to normal saline yet having a hypoxic oxygen concentration between about 0% to about 5% of said maximal dissolved oxygen capacity. The bag oxygen concentration level remains low when the apparatus is stored at 22°-25° C. in normal atmospheric conditions for a period of at least 30 days. The present invention simplifies the process of achieving donor cell hypoxic preconditioning for cell implantation, and may be used to bathe said cells to be transplanted for a sufficient time to activate the hypoxic metabolic pathway.

    摘要翻译： 公开了一种用于制备用于植入生物体的细胞的装置。 该装置包括基本上不透气态的容器; 和所述容器内的流体，所述流体具有基本上等于生理盐水的最大溶解氧能力，但是具有在所述最大溶解氧容量的约0％至约5％之间的缺氧氧浓度。 当装置在正常大气条件下在22°-25℃下储存至少30天时，袋氧浓度水平保持较低。 本发明简化了用于细胞植入的供体细胞缺氧预处理的过程，并且可以用于洗涤待移植的细胞足够的时间以激活缺氧代谢途径。

    Apparatus and method for separating and isolating components of a biological fluid
    10.
    发明申请
    Apparatus and method for separating and isolating components of a biological fluid 有权
    用于分离和分离生物流体组分的装置和方法

    公开(公告)号：US20120128552A1

    公开(公告)日：2012-05-24

    申请号：US13374988

    申请日：2012-01-24

    申请人： John R. Chapman ,  Vijay Kumar ,  Brian K. Cinquini ,  Phillip D. Kingsley

    发明人： John R. Chapman ,  Vijay Kumar ,  Brian K. Cinquini ,  Phillip D. Kingsley

    IPC分类号： B04B1/00

    CPC分类号： B01D21/26 ,  A61M1/029 ,  A61M2202/0413 ,  A61M2202/0464 ,  A61M2202/10 ,  B01D21/262 ,  B01D21/307 ,  B01D21/34 ,  B01D2221/10 ,  B01L3/50215 ,  B01L2300/048 ,  B01L2300/049 ,  B01L2300/0681 ,  B01L2400/0605 ,  B01L2400/0611 ,  G01N2035/00495

    摘要： A device for separating and isolating components of a biological fluid comprising a container for containing the fluid to be processed, a tube cap assembly for closing the container while providing filling and extraction communication therewith, a float assembly disposed within the container for funneling and controlling biological fluid flow into an inverted domed shaped isolation chamber within the float and controlling the biological fluid flow out of the isolation chamber for effecting an encapsulation or a sealed isolation of at least one component or fraction of the biological fluid flow within the isolation chamber during a centrifugation process. The device further comprising a flexible tube for connecting an extraction passageway disposed within the float assembly and an extraction valve of the tube cap assembly for allowing extraction of at least the one component or fraction encapsulated or isolated within the chamber.

    摘要翻译： 一种用于分离和分离生物流体组分的装置，包括用于容纳待处理流体的容器，用于在提供与其进行填充和抽出连通的同时关闭容器的管帽组件;设置在容器内用于漏斗和控制生物 流体流入浮子内的反向圆顶形隔离室，并控制生物流体流出隔离室，用于在离心过程中进行隔离室内至少一种组分或分离的生物流体流的密封隔离 处理。 所述装置还包括用于连接设置在所述浮子组件内的抽吸通道的柔性管和所述管帽组件的抽吸阀，用于允许至少提取在所述室内封装或隔离的所述一个部件或部件。