-
公开(公告)号：US08420107B2
公开(公告)日：2013-04-16
申请号：US13427477
申请日：2012-03-22
IPC分类号： A61K39/09 , C07K14/315
CPC分类号： A61K39/092 , A61K39/00 , C07K14/001 , C07K14/315 , C07K2319/00
摘要： This invention, in one aspect, relates to synthetic immunoreactive peptides. These peptides are approximately 20-25 amino acids in length which are portions of the N termini of the M proteins of the most prevalent United States (U.S.) Group A Streptococcus (GAS) serotypes. At least some of the synthetic peptides can be recognized by M type-specific antibodies and are capable of eliciting functional opsonic antibodies and/or anti-attachment antibodies without eliciting tissue cross-reactive antibodies. In another aspect, it relates to compositions or vaccines comprising these synthetic serotype-specific peptides, including polypeptides and proteins. The invention may also be isolated antibodies which are raised in response to the peptides, compositions or vaccines. The invention further relates to kits for using the peptides, compositions, or antibodies. In still further aspects, the invention also relates to methods for using the peptides, compositions, vaccines, or antibodies and methods for tailoring vaccines.
摘要翻译： 本发明在一个方面涉及合成免疫反应肽。 这些肽的长度约为20-25个氨基酸，它们是最流行的美国（A）链球菌（GAS）血清型的M蛋白的N末端的部分。 至少一些合成肽可被M型特异性抗体识别，并且能够引发功能性调理抗体和/或抗附着抗体而不引发组织交叉反应性抗体。 另一方面，本发明涉及包含这些合成血清型特异性肽（包括多肽和蛋白质）的组合物或疫苗。 本发明还可以是响应于肽，组合物或疫苗引起的分离的抗体。 本发明还涉及使用肽，组合物或抗体的试剂盒。 在另外的方面，本发明还涉及使用肽，组合物，疫苗或抗体的方法和用于定制疫苗的方法。
-
公开(公告)号：US07960535B2
公开(公告)日：2011-06-14
申请号：US12619357
申请日：2009-11-16
CPC分类号： C07K14/3156 , A61K39/00 , C07K2319/00 , Y02A50/401
摘要： The present invention relates to recombinant lipidated PsaA proteins and recombinant constructs from which such lipidated PsaA proteins may be expressed. The invention relates further to lipidated PsaA proteins in which lipidation is effected by the use of a heterologous leader sequence derived from the ospA gene of Borrelia burgdorferi, which leader sequence is joined in translational reading frame with the psaA structural gene. The invention also provides methods of preparation of lipidated PsaA proteins and use of such proteins in immunological compositions. Also provided are vaccines comprising immunogenic lipidated PsaA proteins and methods of use of such vaccines in the prevention and treatment of S. pneumoniae infection.
摘要翻译： 本发明涉及重组脂化的PsaA蛋白和重组构建体，其中可以表达这种脂质化的PsaA蛋白。 本发明进一步涉及脂质化PsaA蛋白，其中通过使用源自博氏疏螺旋体的ospA基因的异源前导序列来实现脂化，该引导序列与psaA结构基因连接在翻译阅读框中。 本发明还提供了脂质化PsaA蛋白的制备方法和这些蛋白质在免疫组合物中的应用。 还提供了包含免疫原性脂化PsaA蛋白的疫苗以及这种疫苗在预防和治疗肺炎链球菌感染中的使用方法。
-
公开(公告)号：US07883710B2
公开(公告)日：2011-02-08
申请号：US12144461
申请日：2008-06-23
CPC分类号： A61K39/092 , A61K39/00 , C07K14/001 , C07K14/315 , C07K2319/00
摘要： This invention, in one aspect, relates to synthetic immunoreactive peptides. These peptides are approximately 20-25 amino acids in length which are portions of the N termini of the M proteins of the most prevalent United States (U.S.) Group A Streptococcus (GAS) serotypes. At least some of the synthetic peptides can be recognized by M type-specific antibodies and are capable of eliciting functional opsonic antibodies and/or anti-attachment antibodies without eliciting tissue cross-reactive antibodies. In another aspect, it relates to compositions or vaccines comprising these synthetic serotype-specific peptides, including polypeptides and proteins. The invention may also be isolated antibodies which are raised in response to the peptides, compositions or vaccines. The invention further relates to kits for using the peptides, compositions, or antibodies. In still further aspects, the invention also relates to methods for using the peptides, compositions, vaccines, or antibodies and methods for tailoring vaccines.
摘要翻译： 本发明在一个方面涉及合成免疫反应肽。 这些肽的长度约为20-25个氨基酸，它们是最流行的美国（A）链球菌（GAS）血清型的M蛋白的N末端的部分。 至少一些合成肽可被M型特异性抗体识别，并且能够引发功能性调理抗体和/或抗附着抗体而不引发组织交叉反应性抗体。 另一方面，本发明涉及包含这些合成血清型特异性肽（包括多肽和蛋白质）的组合物或疫苗。 本发明还可以是响应于肽，组合物或疫苗引起的分离的抗体。 本发明还涉及使用肽，组合物或抗体的试剂盒。 在另外的方面，本发明还涉及使用肽，组合物，疫苗或抗体的方法和用于定制疫苗的方法。
-
4.发明申请MULTIPLE-VALENT OPSONOPHAGOCYTIC ASSAY SELECTION PANEL ARRAYS AND USES THEREFOR 审中-公开多功能消泡剂测定选择面板阵列及其用途公开(公告)号：US20100099138A1
公开(公告)日：2010-04-22
申请号：US12620772
申请日：2009-11-18
IPC分类号： C12Q1/18
CPC分类号： G01N33/52
摘要： This application discloses a multivalent opsonophagocytosis assay that does not rely on counting of bacterial colonies to determine bacteria viability following opsonophagocytosis. Instead, the method uses a metabolic colorimetric indicator to determine if viable bacteria are present. Also disclosed are arrays that can be used to determine the viability of bacteria following opsonophagocytosis.
摘要翻译： 本申请公开了一种多价调理吞噬作用试验，其不依赖于细菌菌落的计数以确定调理吞噬作用后的细菌活力。 相反，该方法使用代谢比色指示剂来确定是否存在活细菌。 还公开了可以用于确定调理吞噬作用后细菌生存力的阵列。
-
5.发明授权公开(公告)号：US07659085B2
公开(公告)日：2010-02-09
申请号：US10259907
申请日：2002-09-27
IPC分类号： G01N33/554
CPC分类号： G01N33/54313 , G01N33/56927 , G01N33/56944 , G01N33/582 , G01N33/585 , Y10S435/971 , Y10S435/973 , Y10T436/101666 , Y10T436/13
摘要： Methods and compositions comprising immunoassays for the detection of antigens and antibodies in a sample are described. In particular, the present invention provides assays that are useful for the rapid and simultaneous detection of multiple different antigens and antibodies. In preferred embodiments, the assays include fluorescent labels of multiple wavelengths or intensities, which are used to label the antigens and antibodies directly and to label beads coated with molecules specific for the antigen or antibody. The detection of a fluorescence shift indicates the presence or identity of the antigen or antibody in the sample.
摘要翻译： 描述了包含用于检测样品中抗原和抗体的免疫测定法的方法和组合物。 特别地，本发明提供了可用于快速和同时检测多种不同抗原和抗体的测定法。 在优选实施方案中，测定包括多个波长或强度的荧光标记，其用于直接标记抗原和抗体，并标记用抗原或抗体特异性分子包被的珠子。 荧光偏移的检测表明样品中抗原或抗体的存在或同一性。
-
公开(公告)号：US5248594A
公开(公告)日：1993-09-28
申请号：US548011
申请日：1990-07-05
IPC分类号： C07K16/12
CPC分类号： C07K16/126 , Y10S435/96 , Y10S435/975 , Y10S436/804
摘要： A set of three unique monoclonal antibodies have been produced which recognize Legionella with particular specificity, without substantial cross-reactivity with non-Legionella bacteria. These monoclonal antibodies are useful as immunodiagnostic reagents for detecting Legionella.
摘要翻译： 已经产生了一组三种独特的单克隆抗体，其特异性识别军团菌，与非军团菌无相当的交叉反应。 这些单克隆抗体可用作检测军团菌的免疫诊断试剂。
-
7.发明授权Use of a pneumococcal P4 peptide for enhancing opsonophagocytosis in response to a pathogen 有权使用肺炎球菌P4肽来增强针对病原体的调理吞噬作用公开(公告)号：US08431134B2
公开(公告)日：2013-04-30
申请号：US13056522
申请日：2009-07-31
申请人： Edwin W. Ades , Gowrisankar Rajam , Sandra Steiner , George M. Carlone , Nikkoj Melnick , Jacquelyn S. Sampson , Joseph E. Martinez , Julie M. Skinner
发明人： Edwin W. Ades , Gowrisankar Rajam , Sandra Steiner , George M. Carlone , Nikkoj Melnick , Jacquelyn S. Sampson , Joseph E. Martinez , Julie M. Skinner
IPC分类号： A61K39/09 , A61K39/085 , C07K16/00
CPC分类号： A61K38/164 , A61K31/00 , A61K31/53 , A61K38/1725 , A61K39/395 , A61K39/40 , A61K39/42 , A61K45/06 , A61K2300/00
摘要： Methods for enhancing opsonophagocytosis of a pathogen of interest are disclosed. The disclosed methods include administering to a subject an isolated P4 peptide, which includes the amino acid sequence set forth as SEQ ID NO: 1 and optionally an isolated opsonic antibody or a fragment thereof that specifically binds to an antigen present on the surface of the pathogen of interest. In some examples isolated complement protein or a fragment thereof (for example, a C3a, C3b, iC3b, C3d, C4b, or C5a fragment of a complement protein) is also administered. Compositions containing isolated P4 peptide and one or more isolated opsonic antibodies or a fragment thereof that specifically binds to an antigen present of the surface of a pathogen of interest are also disclosed. In some examples, the compositions also include isolated complement protein or fragment thereof, such as one or more of C3a, C3b, iC3b, C3d, C4b, or C5a.
摘要翻译： 公开了增加目的病原体的调理吞噬作用的方法。 所公开的方法包括向受试者施用分离的P4肽，其包括SEQ ID NO：1所示的氨基酸序列和任选的特异性结合存在于病原体表面上的抗原的分离的调理蛋白抗体或其片段 出于兴趣。 在一些实施例中，还给予分离的补体蛋白或其片段（例如，补体蛋白的C3a，C3b，iC3b，C3d，C4b或C5a片段）。 还公开了包含分离的P4肽和一种或多种分离的调理抗体或其特异性结合目的病原体表面存在的抗原的片段的组合物。 在一些实例中，组合物还包括分离的补体蛋白或其片段，例如C3a，C3b，iC3b，C3d，C4b或C5a中的一种或多种。
-
公开(公告)号：US07635486B1
公开(公告)日：2009-12-22
申请号：US09600057
申请日：1999-01-14
CPC分类号： C07K14/3156 , A61K39/00 , C07K2319/00 , Y02A50/401
摘要： The present invention relates to recombinant lipidated PsaA proteins and recombinant constructs from which such lipidated PsaA proteins may be expressed. The invention relates further to lipidated PsaA proteins in which lipidation is effected by the use of a heterologous leader sequence derived from the ospA gene of Borrelia burgdorferi, which leader sequence is joined in translational reading frame with the psaA structural gene. The invention also provides methods of preparation of lipidated PsaA proteins and use of such proteins in immunological compositions. Also provided are vaccines comprising immunogenic lipidated PsaA proteins and methods of use of such vaccines in the prevention and treatment of S. pneumoniae infection.
摘要翻译： 本发明涉及重组脂化的PsaA蛋白和重组构建体，其中可以表达这种脂质化的PsaA蛋白。 本发明进一步涉及脂质化PsaA蛋白，其中通过使用源自博氏疏螺旋体的ospA基因的异源前导序列来实现脂化，该引导序列与psaA结构基因连接在翻译阅读框中。 本发明还提供了脂质化PsaA蛋白的制备方法和这些蛋白质在免疫组合物中的应用。 还提供了包含免疫原性脂化PsaA蛋白的疫苗以及这种疫苗在预防和治疗肺炎链球菌感染中的使用方法。
-
9.发明申请Functional Epitopes of Streptococcus Pneumoniae PsaA Antigen and Uses Thereof 有权肺炎链球菌PsaA抗原的功能表位及其用途公开(公告)号：US20080305123A1
公开(公告)日：2008-12-11
申请号：US11992719
申请日：2005-07-29
申请人： Edwin W. Ades , Jacquelyn S. Sampson , Sandra Steiner , George M. Carlone , Joseph J. Caba , GowriSankar Rajam
发明人： Edwin W. Ades , Jacquelyn S. Sampson , Sandra Steiner , George M. Carlone , Joseph J. Caba , GowriSankar Rajam
CPC分类号： C07K14/3156 , A61K39/00 , A61K2039/505 , Y10S530/825
摘要： Provided is a P4 peptide, which contains functional epitopes of the PsaA protein of Streptococcus pneumoniae, and related methods and compositions. A peptide that comprises the amino acid sequence defined in SEQ ID NO:1 (an example of the P4 peptide) is provided. Also provided is a peptide comprising the amino acid sequence defined as VPSLFVDSSVDDRPMKTVSQDTNIPIYAQIFTDS (SEQ ID NO:2). P4 peptide mimetics having a conformational structure identical or similar to the conformation of P4 (e.g., SEQ ID NO: 1 and SEQ ID NO:2) are provided. An antibody that specifically binds to the epitope defined by the disclosed peptides is provided. For example, the antibody can specifically bind to a peptide comprising the sequence set forth in SEQ ID NO: 1 and having the conformation defined by the peptide consisting of SEQ ID NO: 1. An antibody that specifically binds to a peptide comprising the sequence set forth in SEQ ID NO:2 and having the conformation defined by the peptide consisting of SEQ ID NO:2 is also provided. A P4-specific antibody is PsaA-specific since P4 defines an epitope specific for PsaA. A vaccine comprising the peptide of SEQ ID NO: 1 and a pharmaceutical carrier is provided. A vaccine comprising a peptide of SEQ ID NO:2 and a pharmaceutical carrier is also provided. Methods of using the peptides and antibodies of the invention are provided. Diagnostic kits comprising a P4 peptide is also provided.
摘要翻译： 提供了含有肺炎链球菌PsaA蛋白的功能性表位的P4肽及其相关方法和组合物。 提供了包含SEQ ID NO：1中定义的氨基酸序列（P4肽的实例）的肽。 还提供了包含定义为VPSLFVDSSV DDRPMKTVSQDTNIPIYAQIFTDS（SEQ ID NO：2）的氨基酸序列的肽。 提供了具有与P4的构象相同或相似的构象结构的P4肽模拟物（例如，SEQ ID NO：1和SEQ ID NO：2）。 提供了特异性结合由公开的肽定义的表位的抗体。 例如，抗体可以特异性结合包含SEQ ID NO：1所示序列并具有由SEQ ID NO：1组成的肽所定义的构象的肽。特异性结合包含序列集的肽的抗体 还提供了具有由SEQ ID NO：2组成的肽定义的构象的SEQ ID NO：2中的SEQ ID NO：2。 P4特异性抗体是PsaA特异性的，因为P4定义了PsaA特异性表位。 提供了包含SEQ ID NO：1的肽和药物载体的疫苗。 还提供了包含SEQ ID NO：2的肽和药物载体的疫苗。 提供使用本发明的肽和抗体的方法。 还提供了包含P4肽的诊断试剂盒。
-
10.发明授权Streptococcus pneumoniae 37-KDA surface adhesin a protein and nucleic acids coding therefor 失效肺炎链球菌37-KDA表面粘附素a蛋白及其编码的核酸
公开(公告)号：US5854416A
公开(公告)日：1998-12-29
申请号：US715131
申请日：1996-09-17
CPC分类号： C07K14/3156 , C07K16/1275 , A61K39/00
摘要： The invention provides a nucleic acid encoding the 37-kDa protein from Streptococcus pneumoniae. Also provided are isolated nucleic acids comprising a unique fragment of at least 10 nucleotides of the 37-kDa protein. The invention also provides purified polypeptides encoded by the nucleic acid encoding the 37-kDa protein from and the nucleic acids comprising a unique fragment of at least 10 nucleotides of the 37-kDa protein. Also provided are antibodies which selectively binds the polypeptides encoded by the nucleic acid encoding the 37-kDa protein and the nucleic acids comprising a unique fragment of at least 10 nucleotides of the 37-kDa protein. Also provided are vaccines comprising immunogenic polypeptides encoded by the nucleic acid encoding the 37-kDa protein and the nucleic acids comprising a unique fragment of at least 10 nucleotides of the 37-kDa protein. Further provided is a method of detecting the presence of Streptococcus pneumoniae in a sample comprising the steps of contacting a sample suspected of containing Streptococcus pneumoniae with nucleic acid primers capable of hybridizing to a nucleic acid comprising a portion of the nucleic acid encoding the 37-kDa protein, amplifying the nucleic acid and detecting the presence of an amplification product, the presence of the amplification product indicating the presence of Streptococcus pneumoniae in the sample. Further provided are methods of detecting the presence of Streptococcus pneumoniae in a sample using antibodies or antigens, methods of preventing and treating Streptococcus pneumoniae infection in a subject.
摘要翻译： 本发明提供编码来自肺炎链球菌的37-kDa蛋白质的核酸。 还提供了分离的核酸，其包含37-kDa蛋白质的至少10个核苷酸的独特片段。 本发明还提供由编码37-kDa蛋白的核酸编码的纯化多肽，其核酸包含37-kDa蛋白质的至少10个核苷酸的独特片段。 还提供了选择性结合由编码37-kDa蛋白的核酸编码的多肽的抗体和包含37-kDa蛋白质的至少10个核苷酸的独特片段的核酸。 还提供了疫苗，其包含由编码37-kDa蛋白质的核酸编码的免疫原性多肽和包含37-kDa蛋白质至少10个核苷酸的独特片段的核酸。 进一步提供一种检测样品中肺炎链球菌存在的方法，包括以下步骤：将疑似含有肺炎链球菌的样品与能够与编码37kDa的核酸的一部分核酸的核酸引物接触 蛋白质，扩增核酸并检测扩增产物的存在，显示样品中存在显示肺炎链球菌存在的扩增产物。 还提供了使用抗体或抗原检测样品中肺炎链球菌存在的方法，在受试者中预防和治疗肺炎链球菌感染的方法。
-
-
-
-
-
-
-
-
-
搜索结果
29 条记录 (耗时 0.02 秒)
