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公开(公告)号：US07247429B2
公开(公告)日：2007-07-24
申请号：US10212732
申请日：2002-08-07
CPC分类号： C12Q1/6848 , B01L99/00 , B08B7/005 , B08B7/0057 , G01N35/1004 , G01N2035/00277 , C12Q2523/313
摘要： A method of cleaning a pin head or other implements between handling a plurality of samples of nucleic acid subject to an amplification process using a biochemical amplification product. The method comprises exposing the pin head or other implement to ultraviolet (UV) and preferably also infrared (IR) illumination between handling different samples in order to suppress cross-contamination by non-specific amplification. The biochemical amplification product may be obtained from a rolling circle amplification (RCA) procedure, such as that catalyzed by bacteriophage phi29, or a polymerase chain reaction (PCR) amplification product.
摘要翻译： 一种在处理使用生物化学扩增产物进行扩增过程的多个核酸样本之间清洁针头或其它器具的方法。 该方法包括在处理不同样品之间将针头或其他器具暴露于紫外线（UV），并且优选地也是红外（IR）照明，以便通过非特异性扩增来抑制交叉污染。 生物化学扩增产物可以从滚环扩增（RCA）程序获得，例如通过噬菌体phi29催化的或聚合酶链反应（PCR）扩增产物。
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公开(公告)号：US06752182B2
公开(公告)日：2004-06-22
申请号：US09892614
申请日：2001-06-28
IPC分类号： B65B104
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00387 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00659 , B01J2219/00677 , B01J2219/00689 , B01J2219/00691 , B01L3/0244 , B01L9/547 , B01L2200/025 , B01L2200/143 , B01L2400/025 , C40B60/14 , G01N35/1011
摘要： The invention is based on the realization that feedback control for the vertical drive of a pin head becomes unnecessary if one instead prealigns the relative heights of the spotting surface and the well plates such that the pins in the pin head are lowered to the same absolute height both for picking up liquid from the well plates and depositing it onto the spotting surface. By aligning the heights of the well plates and spotting surfaces, the vertical drive of the pin head needs only to be able to reproducibly and accurately arrive at a single lowered position. A pin head can provide this functionality by having a mechanically defined bottom point, obviating the need for a complex and costly feedback control system for height determination of the pins. A simple, low-cost micro-arrayer can thus be provided.
摘要翻译： 本发明基于这样一种认识，即如果一个针对喷头表面和井板的相对高度预先对准，使得销头中的销降低到相同的绝对高度，则针头的垂直驱动的反馈控制变得不必要 两者用于从井板拾取液体并将其沉积在点样表面上。 通过对准井板和点样表面的高度，针头的垂直驱动仅需要能够再现和准确地到达单个降低的位置。 针头可以通过具有机械限定的底点来提供该功能，从而避免需要用于销的高度确定的复杂且昂贵的反馈控制系统。 因此可以提供简单，低成本的微阵列器。
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公开(公告)号：US06878949B2
公开(公告)日：2005-04-12
申请号：US10225302
申请日：2002-08-22
IPC分类号： G01N21/64 , G01N27/447
CPC分类号： G01N27/44721 , G01N21/6456 , G01N27/44773
摘要： A robot for imaging and excision of two-dimensional fluorescent gels. An array detector is mounted in the roof of the robot to image down onto a gel dish placed on a light table. Fluorescence is excited by illuminating the light table from below with light emitting diodes mounted under the main bed of the apparatus. It is therefore possible to perform gel fluorescence analysis and coring (excision) using the same machine. The integration is based on a transmission mode optical design, distinct from prior art scanners and imagers which are based on reflection mode designs. This approach allows the conventional layout of a microarraying or picking robot to be maintained, avoiding unnecessary redesign of other robot functions associated with well plate and container handling. The apparatus may also be used for contrast imaging of non-fluorescent gels.
摘要翻译： 用于成像和切除二维荧光凝胶的机器人。 阵列检测器安装在机器人的屋顶上，以便成像到放置在光台上的凝胶皿上。 通过用安装在设备主床下方的发光二极管照射来自下面的光台来激发荧光。 因此，可以使用相同的机器进行凝胶荧光分析和取芯（切除）。 该集成基于与基于反射模式设计的现有技术扫描仪和成像器不同的传输模式光学设计。 这种方法允许维护微阵列或拾取机器人的传统布局，避免不必要地重新设计与井板和容器处理相关的其他机器人功能。 该装置还可用于非荧光凝胶的对比成像。
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