公开(公告)号：US6071878A

公开(公告)日：2000-06-06

申请号：US990094

申请日：1997-12-12

申请人： Armelle Delecluse ,  Andre Klier ,  Georges Rapoport

发明人： Armelle Delecluse ,  Andre Klier ,  Georges Rapoport

IPC分类号： A01N63/02 ,  C07K14/325 ,  C12N1/21 ,  C12N15/32

CPC分类号： C12R1/075 ,  C07K14/325 ,  Y10S435/832 ,  Y10S530/825

摘要： The cytA gene encoding the 28 kDa polypeptide of Bacillus thuringiensis israelensis crystals was disrupted in the 72 MDa resident plasmid by in vivo recombination. The absence of the 28 kDa protein in B. thuringiensis israelensis did not affect the crystallization of the other toxic components of the parasporal body. However, the absence of the 28 kDa protein did abolish the hemolytic activity of B. thuringiensis serovar israelensis crystals. The mosquitocidal activity of the 28 kDa protein-free crystals did not differ significantly from the wild-type crystals.

摘要翻译： 编码苏云金芽孢杆菌以色列结晶28kDa多肽的cytA基因通过体内重组在72个MDa定居质粒中被破坏。 苏云金芽孢杆菌以色列中28kDa的蛋白质不存在，不影响其他有毒成分的结晶。 然而，28kDa蛋白质的缺失确实消除了苏云金芽孢杆菌血清型以色列晶体的溶血活性。 28 kDa无蛋白结晶的杀昆虫活性与野生型晶体没有显着差异。

公开(公告)号：US5304484A

公开(公告)日：1994-04-19

申请号：US760075

申请日：1991-09-13

申请人： Armelle Delecluse ,  Andre Klier ,  Georges Rapoport

发明人： Armelle Delecluse ,  Andre Klier ,  Georges Rapoport

IPC分类号： A01N63/02 ,  C07K14/325 ,  C12N1/21 ,  C12N15/32 ,  C12N15/75

CPC分类号： C12R1/075 ,  C07K14/325 ,  Y10S435/832 ,  Y10S530/825

摘要： The cyta gene encoding the 28 kDa polypeptide of Bacillus thuringiensis israelensis crystals was disrupted in the 72 MDa resident plasmid by in vivo recombination. The absence of the 28 kDa protein in B. thuringiensis israelensis did not affect the crystallization of the other toxic components of the parasporal body. However, the absence of the 28 kDa protein did abolish the hemolytic activity of B. thuringiensis serovar israelensis crystals. The mosquitocidal activity of the 28 kDa protein-free crystals did not differ significantly from the wild-type crystals.

摘要翻译： 编码苏云金芽孢杆菌以色列结晶的28kDa多肽的cyta基因通过体内重组在72个MDa驻留质粒中被破坏。 苏云金芽孢杆菌以色列中28kDa的蛋白质不存在，不影响其他有毒成分的结晶。 然而，28kDa蛋白质的缺失确实消除了苏云金芽孢杆菌血清型以色列晶体的溶血活性。 28 kDa无蛋白结晶的杀昆虫活性与野生型晶体没有显着差异。

公开(公告)号：US5736513A

公开(公告)日：1998-04-07

申请号：US149950

申请日：1993-11-10

申请人： Armelle Delecluse ,  Andre Klier ,  Georges Rapoport

发明人： Armelle Delecluse ,  Andre Klier ,  Georges Rapoport

IPC分类号： A01N63/02 ,  C07K14/325 ,  C12N1/21 ,  C12N15/32

CPC分类号： C12R1/075 ,  C07K14/325 ,  Y10S435/832 ,  Y10S530/825

摘要： The cytA gene encoding the 28 kDa polypeptide of Bacillus thuringiensis israelensis crystals was disrupted in the 72 MDa resident plasmid by in vivo recombination. The absence of the 28 kDa protein in B. thuringiensis israelensis did not affect the crystallization of the other toxic components of the parasporal body. However, the absence of the 28 kDa protein did abolish the hemolytic activity of B. thuringiensis serovar israelensis crystals. The mosquitocidal activity of the 28 kDa protein-free crystals did not differ significantly from the wild-type crystals.

公开(公告)号：US5264350A

公开(公告)日：1993-11-23

申请号：US798366

申请日：1991-11-21

申请人： Antoine P. Amory ,  Georges Rapoport ,  Frederik Kunst ,  Andre Klier ,  Raymond Dedonder

发明人： Antoine P. Amory ,  Georges Rapoport ,  Frederik Kunst ,  Andre Klier ,  Raymond Dedonder

IPC分类号： C07K14/32 ,  C12N9/24 ,  C12N9/26 ,  C12N9/54 ,  C12N15/75 ,  C12P21/00 ,  C07K7/00 ,  C12N15/00 ,  C12N15/31 ,  C12P21/02

CPC分类号： C12Y302/01008 ,  C07K14/32 ,  C12N15/75 ,  C12N9/2408 ,  C12N9/2414 ,  C12N9/2417 ,  C12N9/54 ,  C12Y204/0101 ,  C12Y302/01001 ,  C12Y302/01032 ,  C12Y302/01039 ,  C12Y302/01073 ,  C07K2319/036

摘要： The invention relates to any fragment of DNA derived from the genome of B. licheniformis coding for a polypeptide of 46 amino acids, or for any fragment of this polypeptide possessing the property of stimulating the production of enzymes secreted by B. subtilis by introduction of the above-mentioned fragment of DNA into this latter by the intermediary of a plasmid.The invention also relates to a procedure for the stimulation of the production of a specific polypeptide by B. subtilis by introduction of the above-mentioned fragment of DNA in a strain of B. subtilis, the genome of which has been modified so that it contains a sequence of DNA coding for the said specific polypeptide.

摘要翻译： 本发明涉及编码46个氨基酸的多肽的地衣芽孢杆菌的基因组DNA的任何片段，或该多肽的任何片段，其具有刺激枯草芽孢杆菌分泌的酶的产生的性质，通过引入 上述片段的DNA通过质粒的中间体转化成后者。 本发明还涉及通过在枯草芽孢杆菌菌株中引入上述DNA片段来刺激枯草芽孢杆菌特异性多肽的产生的方法，该基因组已被修饰以使其含有 编码所述特异性多肽的DNA序列。

公开(公告)号：US5928891A

公开(公告)日：1999-07-27

申请号：US487644

申请日：1995-06-07

申请人： Andre Klier ,  Georges Rapoport ,  Raymond Dedonder

发明人： Andre Klier ,  Georges Rapoport ,  Raymond Dedonder

IPC分类号： A01N63/02 ,  C07K14/325 ,  C12N1/21 ,  C12N15/32 ,  C12N15/75 ,  C12P1/04 ,  C12N15/70 ,  C12N15/74

CPC分类号： C12N15/75 ,  A01N63/02 ,  C07K14/325 ,  C12P1/04

摘要： The invention relates to a vector for transforming microorganisms capable of undergoing sporulation and which contains itself a heterologous insert comprising a DNA sequence coding for at least part of crystal protein, particularly that of B. thuringensis. It also concerns the polypeptides expressed by said microorganisms and having insecticidal properties similar to those of crystal protein, the microorganisms themselves, as transformed by this vector and insecticidal composition including either said polypeptide or the microorganism itself.

摘要翻译： 本发明涉及用于转化能够进行孢子形成的微生物的载体，并且其含有本身含有编码至少部分晶体蛋白质，特别是苏云金芽孢杆菌的DNA序列的异源插入物。 它还涉及由所述微生物表达的多肽，并且具有与晶体蛋白质微生物本身类似的杀虫性质，微生物本身由该载体转化，并且包含所述多肽或微生物本身的杀虫组合物。