公开(公告)号：US5935859A

公开(公告)日：1999-08-10

申请号：US124802

申请日：1998-07-29

申请人： John Duncan Elliott ,  John Gerald Gleason ,  Jack Dale Leber ,  Gerald C. Simolike

发明人： John Duncan Elliott ,  John Gerald Gleason ,  Jack Dale Leber ,  Gerald C. Simolike

IPC分类号： G01N37/00 ,  B01F13/02 ,  B01J19/00 ,  B01J19/24 ,  C07B61/00 ,  C40B60/14 ,  G01N35/00 ,  G01N35/02 ,  G01N35/10

CPC分类号： G01N35/028 ,  B01F13/0233 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00659 ,  C40B60/14 ,  G01N2035/00574 ,  G01N35/0099 ,  Y10T436/114998 ,  Y10T436/119163 ,  Y10T436/2575

摘要： A mixture of beads from a combinatorial library is held in suspension either by inducing circulating flow of a liquid in a tapered vessel by rising air bubbles or by floating the beads at the interface between two immiscible liquids. Beads are selected individually from the suspension by drawing liquid into the tip of a needle having a circular opening smaller than the diameter of a bead. After a bead is captured on the tip of a needle, excess beads are jarred loose or washed away by flow of a solvent through a sheath surrounding the needle, and the needle is moved by a robotic manipulator to a well into which the bead is released.

摘要翻译： 来自组合文库的珠子的混合物通过在气泡中升高而使锥形容器中的液体循环流动或通过在两种不混溶的液体之间的界面处漂浮珠子来保持悬浮液。 通过将液体引入具有比珠的直径小的圆形开口的针的尖端中，从悬浮液中分别选择珠粒。 在针尖上捕获珠粒后，通过溶剂流过围绕针的护套使多余的珠子松动或冲洗掉，并且针由机器人操纵器移动到珠被释放的孔中 。