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    Method for predicting progression free and overall survival at each follow-up time point during therapy of metastatic breast cancer patients using circulating tumor cells
    1.
    发明申请
    Method for predicting progression free and overall survival at each follow-up time point during therapy of metastatic breast cancer patients using circulating tumor cells 审中-公开
    用于预测使用循环肿瘤细胞的转移性乳腺癌患者治疗期间每个随访时间点的无进展和总生存的方法

    公开(公告)号：US20090061456A1

    公开(公告)日：2009-03-05

    申请号：US11897504

    申请日：2007-08-30

    申请人： William J. Allard ,  Gerald V. Doyle ,  Daniel F. Hayes ,  Michael Craig Miller ,  Leon W.M.M Terstappen

    发明人： William J. Allard ,  Gerald V. Doyle ,  Daniel F. Hayes ,  Michael Craig Miller ,  Leon W.M.M Terstappen

    IPC分类号： G01N33/53

    CPC分类号： G01N33/56966 ,  G01N2800/56

    摘要： A cancer test having prognostic utility in predicting time to disease progression, overall survival, and response to therapy in patients with MBC based upon the presence and number of CTC's. The Cell Spotter® System is used to enumerate CTC's in blood. The system immunomagnetically concentrates epithelial cells, fluorescently labels the cells and identifies and quantifies CTC's. The absolute number of CTC's detected in the peripheral blood tumor load is, in part, a factor in prediction of survival, time to progression, and response to therapy. The mean time to survival of patients depended upon a threshold number of 5 CTC's per 7.5 ml of blood. Detection of CTC's in metastatic cancer represents a novel prognostic factor in patients with metastatic cancers, suggests a biological role for the presence of tumor cells in the blood, and indicates that the detection of CTC's could be considered an appropriate surrogate marker for prospective therapeutic clinical trials.

    摘要翻译： 根据CTC的存在和数量，可以预测预测用于预测MBC患者疾病进展时间，总体生存期和对治疗反应的预后效果。 Cell Spotter（R）系统用于列举血液中的四氯化碳。 该系统免疫磁性浓缩上皮细胞，荧光标记细胞，并识别和量化CTC。 在外周血肿瘤负荷中检测到的四氯化碳的绝对数量部分是预测生存期，进展时间和治疗反应的因素。 患者生存的平均时间取决于每7.5毫升血液的5个CTC的阈值数。 在转移性癌症中检测CTC代表转移癌患者的新型预后因素，表明血液中肿瘤细胞存在的生物学作用，并且表明CTC检测可被认为是前瞻性治疗性临床试验的适当替代标记 。

    Apparatus and methods for magnetic separation
    2.
    发明授权
    Apparatus and methods for magnetic separation 有权
    用于磁分离的装置和方法

    公开(公告)号：US06361749B1

    公开(公告)日：2002-03-26

    申请号：US09376686

    申请日：1999-08-18

    申请人： Leon M. M. Terstappen ,  Gerald V. Doyle ,  Paul A. Liberti ,  Gerald J. Dolan

    发明人： Leon M. M. Terstappen ,  Gerald V. Doyle ,  Paul A. Liberti ,  Gerald J. Dolan

    IPC分类号： G01N33553

    CPC分类号： B03C1/035 ,  B03C1/0332 ,  B03C1/286 ,  C02F1/48 ,  C02F1/488 ,  G01N35/0098 ,  G01N2446/00 ,  Y10S435/971 ,  Y10S436/807 ,  Y10S436/824 ,  Y10S436/827 ,  Y10S436/828 ,  Y10T436/24

    摘要： Separation apparatus and method for separating magnetic and/or magnetically-labeled particles from a test medium in a reaction chamber which incorporates baffles, a spinner, a stirrer, or a plunger operable to be displaced along a collection surface to define a narrow flow path through which the particle-laden test medium must flow. The collection surface is a thin-walled non-magnetic material having a plurality of magnetic pole faces positioned therearound. Test medium within a reaction chamber is caused to flow past a collecting surface, and a high-gradient magnetic field is applied to the surface to capture magnetically responsive particles in the test medium. The particles are deflected toward the collection surface in the flow path for the test medium. The narrow flow path assures that substantially all of the particles pass within the high-gradient magnetic field along the collection surface. The particles are normally suspended in the medium, and are separated out of suspension by adhesion to the collection surface. The particles may be resuspended by removal of the surface from the high-gradient field, or removal of the high-gradient field from the surface.

    摘要翻译： 用于将反应室中的磁性和/或磁性标记的颗粒与测试介质分离的分离装置和方法，该反应室包括挡板，旋转器，搅拌器或柱塞，其可操作地沿着收集表面移位以限定通过 颗粒载体测试介质必须流动。 收集表面是具有定位在其周围的多个磁极面的薄壁非磁性材料。 使反应室内的测试介质流过收集表面，并将高梯度磁场施加到表面以捕获测试介质中的磁响应颗粒。 颗粒在用于测试介质的流动路径中朝向收集表面偏转。 窄流动路径确保基本上所有的颗粒沿着收集表面通过高梯度磁场。 颗粒通常悬浮在介质中，并通过粘附到收集表面而从悬浮液中分离出来。 可以通过从高梯度场去除表面或从表面去除高梯度场来重新悬浮颗粒。

    Pre-clinical method for monitoring serial changes in circulating breast cancer cells in mice
    3.
    发明申请
    Pre-clinical method for monitoring serial changes in circulating breast cancer cells in mice 审中-公开
    用于监测小鼠循环乳腺癌细胞的连续变化的临床前方法

    公开(公告)号：US20090117532A1

    公开(公告)日：2009-05-07

    申请号：US12254151

    申请日：2008-10-20

    申请人： Gerald V. DOYLE

    发明人： Gerald V. DOYLE

    IPC分类号： A01N1/02

    CPC分类号： G01N33/54326 ,  G01N33/57415

    摘要： The CellTracks® System provides a system to enumerate CTC's in blood. The system immunomagnetically concentrates epithelial cells, fluorescently labels the cells and identifies and quantifies CTC's. The absolute number of CTC's detected in the peripheral blood tumor load is, in part, a factor in prediction of survival, time to progression, and response to therapy. Pre-clinical studies of circulating tumor cells (CTC's) have been limited by the inability to repetitively monitor CTC's in animal models. The present invention provides a method to enumerate CTC's in blood samples obtained from living mice, using a protocol similar to an in vitro diagnostic system for quantifying CTC's in patients. Accordingly, this technology can be adapted for serial monitoring of CTC's in mouse xenograft tumor models of human breast cancer.

    摘要翻译： CellTracks（R）系统提供一个系统来列举血液中的四氯化碳。 该系统免疫磁性浓缩上皮细胞，荧光标记细胞，并识别和量化CTC。 在外周血肿瘤负荷中检测到的四氯化碳的绝对数量部分是预测生存期，进展时间和治疗反应的因素。 循环肿瘤细胞（CTC）的临床前研究受到动物模型中不能重复监测CTC的限制。 本发明提供了一种使用类似于用于定量患者中的CTC的体外诊断系统的方案来列举从活小鼠获得的血液样品中的CTC的方法。 因此，该技术可适用于人乳腺癌小鼠异种移植肿瘤模型中CTC的连续监测。

    Imaging of Activated Vascular Endothelium Using Immunomagnetic MRI Contrast Agents
    7.
    发明申请
    Imaging of Activated Vascular Endothelium Using Immunomagnetic MRI Contrast Agents 审中-公开
    使用免疫磁性MRI造影剂成像激活的血管内皮

    公开(公告)号：US20100297026A1

    公开(公告)日：2010-11-25

    申请号：US12445652

    申请日：2007-11-01

    申请人： Gerald V. Doyle

    发明人： Gerald V. Doyle

    IPC分类号： A61K49/14 ,  A61K49/16

    CPC分类号： A61K49/1875 ,  A61K49/16 ,  A61K49/1869 ,  B82Y5/00

    摘要： Immunomagnetic nanoparticles are used as a contrast agent for enhancing medical diagnostic imaging such as magnetic resonance imaging (MRI). The present invention is directed to methods of making targeted MRI contrast agents from immunomagnetic particles, and to methods of using such MRI contrast agents. Typically, such targeted MRI contrast agents provide enhanced relaxivity, improved signal-to-noise, targeting ability, and resistance to agglomeration. Methods of making such MRI contrast agents typically afford better control over particle size, and methods of using such MRI contrast agents typically can afford enhanced blood clearance rates and distribution. The ability to use the contrast agents im MRI provides a tool in the diagnosis and treatment of several disease states.

    摘要翻译： 使用免疫磁性纳米粒子作为增强医学诊断成像如磁共振成像（MRI）的造影剂。 本发明涉及从免疫磁性颗粒制备靶向MRI造影剂的方法，以及使用这种MRI造影剂的方法。 通常，这种靶向MRI造影剂提供增强的舒张度，改善的信噪比，靶向能力和抵抗聚集。 制备这种MRI造影剂的方法通常能够更好地控制粒度，并且使用这种MRI造影剂的方法通常可以提供增强的血液清除率和分布。 使用造影剂im MRI的能力提供了诊断和治疗几种疾病状态的工具。