Method to Increase the Ethanol Concentration from the Conversion of Lignocellulose
    2.
    发明申请
    Method to Increase the Ethanol Concentration from the Conversion of Lignocellulose 审中-公开
    从木质纤维素转化中提高乙醇浓度的方法

    公开(公告)号:US20110104775A1

    公开(公告)日:2011-05-05

    申请号:US12856073

    申请日:2010-08-13

    IPC分类号: C12P7/10

    CPC分类号: C12P7/10 Y02E50/16 Y02E50/17

    摘要: The present disclosure is related to a method for increasing the ethanol concentration from the conversion of lignocellulose. The pretreated solid residues are mixed with ethanol-containing broth from the fermentation of xylose hydrolysate by Pichia stipitis and then are performed under the process of simultaneous saccharification and fermentation (SSF) with Sacharomyces cerevisiae and cellulase for converting cellulose to ethanol. The final ethanol concentration in broth as well as the ethanol productivity is increased at least 1.8 times in comparison of conventional process for lignocellulosic ethanol production.

    摘要翻译: 本公开涉及一种从木质纤维素转化中提高乙醇浓度的方法。 将经预处理的固体残余物与乙醇培养液混合,通过树干毕赤酵母发酵木糖水解产物,然后在与酿酒酵母和纤维素酶同时糖化和发酵(SSF)的过程中进行纤维素转化为乙醇。 与木质纤维素乙醇生产的常规方法相比,肉汤中的最终乙醇浓度以及乙醇生产率提高至少1.8倍。

    Method of Xylose Fermentation Having Enhanced Conversion Rate in Fiber Solution
    4.
    发明申请
    Method of Xylose Fermentation Having Enhanced Conversion Rate in Fiber Solution 审中-公开
    在纤维素溶液中增加转化率的木糖发酵方法

    公开(公告)号:US20110097780A1

    公开(公告)日:2011-04-28

    申请号:US12822274

    申请日:2010-06-24

    IPC分类号: C12P7/10

    摘要: A method is provided for improving the efficiency of xylose fermentation in lignocellulosic hydrolysate. The disclosed embodiment raises the efficiency of xylose conversion by adding a specific lignocellulosic material during fermentation. In particular, a 10% enhancement in the efficiency of xylose conversion for ethanol production was given, and the ethanol yield is achieved 90% after adding the specific lignocellulosic material.

    摘要翻译: 提供了一种提高木质纤维素水解产物中木糖发酵效率的方法。 所公开的实施方案通过在发酵过程中加入特定的木质纤维素材料提高了木糖转化的效率。 特别地,提供了用于乙醇生产的木糖转化效率的10%提高,并且在加入特定的木质纤维素材料后,乙醇产率达到了90%。

    Cellulose hydrolase and gene thereof
    6.
    发明授权
    Cellulose hydrolase and gene thereof 有权
    纤维素水解酶及其基因

    公开(公告)号:US08518687B2

    公开(公告)日:2013-08-27

    申请号:US13424501

    申请日:2012-03-20

    IPC分类号: C12N1/20 C12N15/00 C07H21/04

    CPC分类号: C12Y302/01091 C12N9/2437

    摘要: A cellulose hydrolase and a gene thereof are obtained by screening a cDNA genomic library constructed with Orpinomyces sp. Y102. The gene is 1071 base pairs long and comprises an open reading frame (ORF) for producing the cellulose hydrolase comprising 357 amino acids by translation. A transformed cell and a carrier carrying the gene are introduced. The gene is transferred to E. coli by transformation, such that E. coli can acquire activity of decomposing CMC, beta-glucan, and xylan. The cellulose hydrolase is multifunctional and is capable of decomposing cellubiose and directly decomposing fiber into glucose.

    摘要翻译: 通过筛选用Orp​​inomyces sp。构建的cDNA基因组文库获得纤维素水解酶及其基因。 Y102。 该基因长1071个碱基对并且包括用于通过翻译产生包含357个氨基酸的纤维素水解酶的开放阅读框(ORF)。 引入转化细胞和携带该基因的载体。 通过转化将基因转移到大肠杆菌中,使得大肠杆菌可以获得分解CMC,β-葡聚糖和木聚糖的活性。 纤维素水解酶是多功能的,并且能够分解纤维素和将纤维直接分解成葡萄糖。

    Cellulose Hydrolase and Gene Thereof
    7.
    发明申请
    Cellulose Hydrolase and Gene Thereof 有权
    纤维素水解酶及其基因

    公开(公告)号:US20130102051A1

    公开(公告)日:2013-04-25

    申请号:US13424501

    申请日:2012-03-20

    IPC分类号: C12N9/42 C12N1/21 C12N15/56

    CPC分类号: C12Y302/01091 C12N9/2437

    摘要: A cellulose hydrolase and a gene thereof are obtained by screening a cDNA genomic library constructed with Orpinomyces sp. Y102. The gene is 1071 base pairs long and comprises an open reading frame (ORF) for producing the cellulose hydrolase comprising 357 amino acids by translation. A transformed cell and a carrier carrying the gene are introduced. The gene is transferred to E. coli by transformation, such that E. coli can acquire activity of decomposing CMC, beta-glucan, and xylan. The cellulose hydrolase is multifunctional and is capable of decomposing cellubiose and directly decomposing fiber into glucose.

    摘要翻译: 通过筛选用Orp​​inomyces sp。构建的cDNA基因组文库获得纤维素水解酶及其基因。 Y102。 该基因长1071个碱基对并且包括用于通过翻译产生包含357个氨基酸的纤维素水解酶的开放阅读框(ORF)。 引入转化细胞和携带该基因的载体。 通过转化将基因转移到大肠杆菌中,使得大肠杆菌可以获得分解CMC,β-葡聚糖和木聚糖的活性。 纤维素水解酶是多功能的,并且能够分解纤维素和将纤维直接分解成葡萄糖。

    METHOD FOR PRODUCING XYLITOL FROM LIGNOCELLULOSIC HYDROLYSATES WITHOUT DETOXIFICATION
    9.
    发明申请
    METHOD FOR PRODUCING XYLITOL FROM LIGNOCELLULOSIC HYDROLYSATES WITHOUT DETOXIFICATION 有权
    在没有脱氧作用的情况下从水杨酸水解产物生产XYLITOL的方法

    公开(公告)号:US20110097772A1

    公开(公告)日:2011-04-28

    申请号:US12775655

    申请日:2010-05-07

    IPC分类号: C12P7/18

    CPC分类号: C12P7/18

    摘要: A method for producing xylitol by fermentation of lignocellulosic hydrolysates without detoxification is provided. By using the originally isolated yeast Candida sp., xylose can be effectively converted into xylitol. The invention also provides the Candida strain having high furfural tolerance, and is capable to produce xylitol from various types of non-detoxified lignocellulosic hydrolysates, in which the overall utilization of xylose in hydrolysate can reach over 95%.

    摘要翻译: 提供了一种通过发酵木质纤维素水解产物而无需解毒来生产木糖醇的方法。 通过使用最初分离的酵母假丝酵母属,木糖可以有效转化成木糖醇。 本发明还提供了具有高糠醛耐性的假丝酵母菌株,并且能够从各种类型的非解毒木质纤维素水解产物中产生木糖醇,其中水解产物中木糖的总体利用可以达到95%以上。

    Method for cleavage of ether-linkages in polyethoxylates
    10.
    发明授权
    Method for cleavage of ether-linkages in polyethoxylates 失效
    在聚乙氧基化物中裂解醚键的方法

    公开(公告)号:US07527964B2

    公开(公告)日:2009-05-05

    申请号:US10874619

    申请日:2004-06-22

    IPC分类号: B09B3/00 C02F3/34 C12N1/20

    摘要: A method for cleavage of the ether-linkage of polyethoxylates is described. A novel pure microbial culture, Pseudomonas nitroreducens TX1 (Depository No.: BCRC910228), is used under aerobic condition to cleave the ether-linkage adjacent to the carboxylated terminus or ethoxyl terminus. This method is useful to cleave sequentially ether-linkage of polyethoxylates in an aqueous buffer solution with an initial concentration of the polyethoxylates between 0.05% and 20%. This method is also effective for the cleavage of the ether-linkages in short-chain polyethoxylates with one to three ethoxyl units.

    摘要翻译: 描述了一种用于裂解聚乙氧基化物的醚键的方法。 在需氧条件下,使用新型纯微生物培养物硝酸还原酶假单胞菌TX1(保藏号:BCRC910228)切割邻近羧基末端或乙氧基末端的醚键。 该方法可用于在聚乙氧基化物的初始浓度为0.05%至20%之间的缓冲溶液中依次切割聚乙氧基化物的醚键。 该方法对于用1-3个乙氧基单元裂解短链多乙氧基化物中的醚键也是有效的。