-
公开(公告)号：US08969064B2
公开(公告)日：2015-03-03
申请号：US13474313
申请日：2012-05-17
IPC分类号： C12N1/00 , C12P21/02 , C12P21/06 , C12N15/64 , C12N15/66 , C12N15/00 , C12N9/90 , C07K14/395 , C07K14/79 , C12N15/81
CPC分类号： C12N15/81 , A61K38/00 , C07K14/395 , C07K14/79 , C07K2319/35 , C12N9/90 , C12N2510/02
摘要： The present invention provides a method for producing a desired protein (such as a desired heterologous protein) comprising: (a) providing a host cell comprising a first recombinant gene encoding a protein comprising the sequence of a first chaperone protein, a second recombinant gene encoding a protein comprising the sequence of a second chaperone protein and a third gene, such as a third recombinant gene, encoding a desired protein (such as a desired heterologous protein), wherein the first and second chaperones are different; and (b) culturing the host cell in a culture medium to obtain expression of the first, second and third genes.
-
公开(公告)号：US20080193977A1
公开(公告)日：2008-08-14
申请号：US11722539
申请日：2005-12-23
CPC分类号： C12N15/81 , A61K38/00 , C07K14/395 , C07K14/79 , C07K2319/35 , C12N9/90 , C12N2510/02
摘要： The present invention provides a method for producing a desired protein (such as a desired heterologous protein) comprising: (a) providing a host cell comprising a first recombinant gene encoding a protein comprising the sequence of a first chaperone protein, a second recombinant gene encoding a protein comprising the sequence of a second chaperone protein and a third gene, such as a third recombinant gene, encoding a desired protein (such as a desired heterologous protein), wherein the first and second chaperones are different; and (b) culturing the host cell in a culture medium to obtain expression of the first, second and third genes.
摘要翻译： 本发明提供了一种生产所需蛋白质（例如所需异源蛋白质）的方法，包括：（a）提供宿主细胞，其包含编码包含第一伴侣蛋白序列的蛋白质的第一重组基因，编码第二分子伴侣蛋白的第二重组基因 包含第二伴侣蛋白和第三基因的序列的蛋白质，例如编码所需蛋白质（例如所需异源蛋白质）的第三重组基因，其中第一和第二伴侣不同; 和（b）在培养基中培养宿主细胞以获得第一，第二和第三基因的表达。
-
公开(公告)号：US09057061B2
公开(公告)日：2015-06-16
申请号：US11722539
申请日：2005-12-23
CPC分类号： C12N15/81 , A61K38/00 , C07K14/395 , C07K14/79 , C07K2319/35 , C12N9/90 , C12N2510/02
摘要： The present invention provides a method for producing a desired protein (such as a desired heterologous protein) comprising: (a) providing a host cell comprising a first recombinant gene encoding a protein comprising the sequence of a first chaperone protein, a second recombinant gene encoding a protein comprising the sequence of a second chaperone protein and a third gene, such as a third recombinant gene, encoding a desired protein (such as a desired heterologous protein), wherein the first and second chaperones are different; and (b) culturing the host cell in a culture medium to obtain expression of the first, second and third genes.
摘要翻译： 本发明提供了一种生产所需蛋白质（例如所需异源蛋白质）的方法，包括：（a）提供宿主细胞，其包含编码包含第一伴侣蛋白序列的蛋白质的第一重组基因，编码第二分子伴侣蛋白的第二重组基因 包含第二伴侣蛋白和第三基因的序列的蛋白质，例如编码所需蛋白质（例如所需异源蛋白质）的第三重组基因，其中第一和第二伴侣不同; 和（b）在培养基中培养宿主细胞以获得第一，第二和第三基因的表达。
-
公开(公告)号：US20120231503A1
公开(公告)日：2012-09-13
申请号：US13474317
申请日：2012-05-17
IPC分类号： C12P21/00 , C12P21/08 , C12N9/72 , C12N1/19 , C12N9/50 , C12N9/04 , C12N9/18 , C12N15/63 , C12N9/74
CPC分类号： C12N15/80
摘要： The present disclosure relates to a method for producing heterologous protein including: (c) providing a host cell comprising a 2 μm-family plasmid, the plasmid comprising a gene encoding a protein comprising the sequence of a chaperone protein and a gene encoding a heterologous protein; (d) culturing the host cell in a culture medium under conditions that allow the expression of the gene encoding the chaperone protein and the gene encoding a heterologous protein; (e) purifying the thus expressed heterologous protein from the cultured host cell or the culture medium.
摘要翻译： 本公开内容涉及用于产生异源蛋白质的方法，包括：（c）提供包含2μm家族质粒的宿主细胞，所述质粒包含编码包含伴侣蛋白序列的蛋白质的基因和编码异源蛋白质的基因 ; （d）在允许表达编码伴侣蛋白的基因和编码异源蛋白质的基因的条件下，在培养基中培养宿主细胞; （e）从培养的宿主细胞或培养基纯化如此表达的异源蛋白质。
-
公开(公告)号：US20070275889A1
公开(公告)日：2007-11-29
申请号：US10584424
申请日：2004-12-23
CPC分类号： C12N15/80
摘要： The present invention provides a method for producing heterologous protein comprising: (a) providing a host cell comprising a 2 μm-family plasmid, the plasmid comprising a gene encoding a protein comprising the sequence of a chaperone protein and a gene encoding a heterologous protein; (b) culturing the host cell in a culture medium under conditions that allow the expression of the gene encoding the chaperone protein and the gene encoding a heterologous protein; (c) purifying the thus expressed heterologous protein from the cultured host cell or the culture medium.
摘要翻译： 本发明提供了产生异源蛋白质的方法，其包括：（a）提供包含2mum家族质粒的宿主细胞，所述质粒包含编码包含伴侣蛋白序列和编码异源蛋白质的基因的蛋白质的基因; （b）在允许表达编码伴侣蛋白的基因和编码异源蛋白质的基因的条件下，在培养基中培养宿主细胞; （c）从培养的宿主细胞或培养基纯化如此表达的异源蛋白质。
-
公开(公告)号：US20120231505A1
公开(公告)日：2012-09-13
申请号：US13474313
申请日：2012-05-17
CPC分类号： C12N15/81 , A61K38/00 , C07K14/395 , C07K14/79 , C07K2319/35 , C12N9/90 , C12N2510/02
摘要： The present invention provides a method for producing a desired protein (such as a desired heterologous protein) comprising: (a) providing a host cell comprising a first recombinant gene encoding a protein comprising the sequence of a first chaperone protein, a second recombinant gene encoding a protein comprising the sequence of a second chaperone protein and a third gene, such as a third recombinant gene, encoding a desired protein (such as a desired heterologous protein), wherein the first and second chaperones are different; and (b) culturing the host cell in a culture medium to obtain expression of the first, second and third genes.
摘要翻译： 本发明提供了一种生产所需蛋白质（例如所需异源蛋白质）的方法，包括：（a）提供宿主细胞，其包含编码包含第一伴侣蛋白序列的蛋白质的第一重组基因，编码第二分子伴侣蛋白的第二重组基因 包含第二伴侣蛋白和第三基因的序列的蛋白质，例如编码所需蛋白质（例如所需异源蛋白质）的第三重组基因，其中第一和第二伴侣不同; 和（b）在培养基中培养宿主细胞以获得第一，第二和第三基因的表达。
-
-
-
-
-
搜索结果
6 条记录 (耗时 0.031 秒)
