公开(公告)号：US11939598B2

公开(公告)日：2024-03-26

申请号：US18319918

申请日：2023-05-18

申请人： Eligo Bioscience

发明人： Jesus Fernandez Rodriguez ,  Antoine Decrulle ,  Aymeric Leveau ,  Ines Canadas Blasco ,  Aurélie Mathieu ,  Thibault Carlier

IPC分类号： C12N7/00 ,  C12N1/20 ,  C12N15/70 ,  C12N15/74

CPC分类号： C12N7/00 ,  C12N1/20 ,  C12N15/70 ,  C12N15/74 ,  C12N2795/10322 ,  C12N2795/10352 ,  C12N2800/101 ,  C12N2800/40

摘要： The present invention concerns a production bacterial cell for producing phage particles or phage-derived delivery vehicles, said production bacterial cell stably comprising at least one phage structural gene(s) and at least one phage DNA packaging gene(s), said phage structural gene(s) and phage DNA packaging gene(s) being derived from a first type of bacteriophage,



wherein the expression of at least one of said phage structural gene(s) and/or at least one of said phage DNA packaging gene(s) in said production bacterial cell is controlled by at least one induction mechanism, and
wherein said production bacterial cell is from a bacterial species or strain different from the bacterial species or strain from which said first type of bacteriophage comes and/or that said first type of bacteriophage targets.

公开(公告)号：US20170218344A1

公开(公告)日：2017-08-03

申请号：US15437820

申请日：2017-02-21

申请人： NATIONAL TAIWAN UNIVERSITY

发明人： Jin Town Wang ,  Tzu Lung Lin

IPC分类号： C12N7/00 ,  C07K14/005

CPC分类号： C12N7/00 ,  C07H21/04 ,  C07K14/005 ,  C12N2795/00021 ,  C12N2795/00022 ,  C12N2795/00032 ,  C12N2795/00033 ,  C12N2795/10221 ,  C12N2795/10222 ,  C12N2795/10232 ,  C12N2795/10233 ,  C12N2795/10322

摘要： The present invention relates to novel bacteriophages specific to Klebsiella pneumoniae strains, and compositions comprising the same. Particularly, polypeptides and their coding nucleic acid molecule of the novel bacteriophages are provided. The invention also relates to applications of the novel bacteriophages and the polypeptides in the detection/treatment/prevention of infection caused by Klebsiella pneumoniae strains. Development of immunogen and vaccine on the basis of the polypeptides are also provided.

公开(公告)号：US20140050713A1

公开(公告)日：2014-02-20

申请号：US14111531

申请日：2012-04-12

申请人： C.B. Appaiah ,  Sriram Padmanabhan ,  R. Sanjeev Saravanan ,  Bharathi Sriram

发明人： C.B. Appaiah ,  Sriram Padmanabhan ,  R. Sanjeev Saravanan ,  Bharathi Sriram

IPC分类号： C12N9/36 ,  C07K14/47

CPC分类号： C12N9/2462 ,  A61K38/00 ,  C07K14/005 ,  C07K14/47 ,  C07K14/4742 ,  C07K2319/03 ,  C12N1/06 ,  C12N9/24 ,  C12N9/48 ,  C12N15/62 ,  C12N2795/00022 ,  C12N2795/00033 ,  C12N2795/10122 ,  C12N2795/10133 ,  C12N2795/10322 ,  C12N2795/10333 ,  C12Y302/01017 ,  Y02A50/475 ,  Y02A50/483

摘要： Provided herein are antibacterial compositions and methods of making and using the compositions.

摘要翻译： 本文提供了抗菌组合物和制备和使用组合物的方法。

公开(公告)号：US20130121967A1

公开(公告)日：2013-05-16

申请号：US13702599

申请日：2011-06-08

申请人： Robert Leah

发明人： Robert Leah

IPC分类号： A61K35/76

CPC分类号： C12N7/00 ,  A61K35/76 ,  C12N2795/00021 ,  C12N2795/00032 ,  C12N2795/00033 ,  C12N2795/00051 ,  C12N2795/10121 ,  C12N2795/10322 ,  C12Q1/04 ,  Y02A50/475 ,  Y02A50/481

摘要： The present invention relates to a composition comprising obligate lytic bacteriophages generated by a method comprising subjecting normally in vivo lysogenic, pseudolysogenic or temperate bacteriophages to genetic modifications in vitro, which alters the biological activity of one or more of the individual gene products for establishing, maintaining, controlling or regulating the lysogenic life cycle of the bacteriophages, thereby converting them to obligate lytic bacteriophages, wherein the genetic modification includes modification of a single gene in the operon containing a gene resulting in a gene product for establishing, maintaining, controlling or regulating the lysogenic life cycle of the bacteriophages.

公开(公告)号：US20110092391A1

公开(公告)日：2011-04-21

申请号：US12991704

申请日：2009-05-11

申请人： Sidney Hayes

发明人： Sidney Hayes

IPC分类号： C40B50/06 ,  C07H21/04 ,  C12N15/63 ,  C12N7/01 ,  C12N7/04

CPC分类号： C12N15/1037 ,  A61K2039/5256 ,  C07K14/005 ,  C07K2319/735 ,  C12N7/00 ,  C12N2795/10321 ,  C12N2795/10322 ,  C40B40/02

摘要： Bacteriophages in general and lambda phage in particular are powerful, flexible reagents who have yet to be exploited to their full potential. As discussed herein, the lambda phage head and/or genome comprises an easy to use and highly efficient delivery vehicle for delivering the expression products of a gene of interest systemically or to a particular tissue.

摘要翻译： 一般的噬菌体和λ噬菌体特别是强大的，灵活的试剂，尚未被充分利用。 如本文所讨论的，λ噬菌体噬菌体头和/或基因组包含易于使用和高效率的递送载体，用于将感兴趣的基因的表达产物全部或特定组织递送。

公开(公告)号：US20070196334A1

公开(公告)日：2007-08-23

申请号：US10561829

申请日：2004-06-25

申请人： Shaharyar Khan

发明人： Shaharyar Khan

IPC分类号： A61K48/00 ,  C12N9/22 ,  C12N15/86 ,  C12N15/74 ,  C12N5/10

CPC分类号： C07K14/005 ,  A61K48/00 ,  C07K2319/07 ,  C07K2319/10 ,  C12N9/127 ,  C12N15/86 ,  C12N2700/00 ,  C12N2795/10322 ,  C12N2795/10343 ,  C12N2795/10345 ,  C12N2800/107 ,  C12N2810/75

摘要： The present disclosure provides compositions and methods for direct transfection of organelle DNA in living cells. More particularly, the present disclosure is based on the use of viral vectors that contain localization signals specific for the target organelle. In one embodiment a viral vector that has been modified to express on its surface a protein transduction domain and an organelle localization signal is provided. A viral vector comprising a desired recombinant DNA construct is introduced into cell culture or injected into an organism, wherein the viral vector transduces across the cellular membrane through its protein transduction domain and localizes to the cellular organelle by way of the organelle localization/targeting signal introducing the recombinant DNA into the interior of the organelle

摘要翻译： 本公开提供了用于在活细胞中直接转染细胞器DNA的组合物和方法。 更具体地，本公开是基于使用包含对靶细胞器特异性的定位信号的病毒载体。 在一个实施方案中，提供已经修饰以在其表面上表达蛋白转导结构域和细胞器定位信号的病毒载体。 将包含所需重组DNA构建体的病毒载体导入细胞培养物或注入生物体，其中病毒载体通过其蛋白质转导结构域转导穿过细胞膜，并通过细胞器定位/靶向信号导入定位于细胞器 重组DNA进入细胞器的内部

公开(公告)号：US07229754B2

公开(公告)日：2007-06-12

申请号：US11286621

申请日：2005-11-23

申请人： Laszlo B. Kish ,  Maria Dobozi-King ,  Ryland Young ,  Mosong Cheng ,  James R. Biard ,  Sergey Bezrukov

发明人： Laszlo B. Kish ,  Maria Dobozi-King ,  Ryland Young ,  Mosong Cheng ,  James R. Biard ,  Sergey Bezrukov

IPC分类号： C12Q1/70

CPC分类号： B82Y30/00 ,  B01L3/5088 ,  C12N7/00 ,  C12N2795/10311 ,  C12N2795/10322 ,  C12N2799/00 ,  C12Q1/04 ,  C12Q1/6825 ,  C12Q1/689 ,  G01N33/48707 ,  G01N33/54373 ,  G01N33/56911

摘要： The present invention provides a method for detecting bacteria and a nano-well device having one or more input/output connections about a gap and one or more bacteriophages at or about the gap that trigger a detectable electrical field fluctuation when the one or more bacteriophages contact a cognate target within a liquid sample.

摘要翻译： 本发明提供一种用于检测细菌的方法和具有一个或多个输入/输出连接的纳米孔装置，所述输入/输出连接围绕间隙和在间隙处或周围的一个或多个噬菌体，当一个或多个噬菌体接触时触发可检测的电场波动 液体样品中的同源靶标。

公开(公告)号：US20060030020A1

公开(公告)日：2006-02-09

申请号：US11073226

申请日：2005-03-03

申请人： Ichiro Maruyama ,  Hiroko Maruyama ,  Sydney Brenner

发明人： Ichiro Maruyama ,  Hiroko Maruyama ,  Sydney Brenner

IPC分类号： C12N7/00 ,  C12N15/74

CPC分类号： C07K14/005 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/20 ,  C07K2319/61 ,  C07K2319/735 ,  C12N9/2471 ,  C12N15/1037 ,  C12N15/62 ,  C12N15/73 ,  C12N2795/10322 ,  C12Y302/01023 ,  C40B40/02

摘要： Lambdoid phage comprising a matrix of proteins encapsulating a genome encoding first and second polypeptides of an autogenously assembling receptor and a receptor comprised of the first and second polypeptides surface-integrated into the matrix via a lambdoid phage tail protein matrix anchor domain fused to at least one of the polypeptides.

摘要翻译： 包含蛋白质基质的蛋白质包含蛋白质基质，所述蛋白质基因组编码自身组装受体的第一和第二多肽，以及由第一和第二多肽组成的受体，所述受体通过与至少一个融合的羊链蛋白噬菌体尾蛋白基质锚结构域表面整合到基质中 的多肽。

公开(公告)号：US20050181506A1

公开(公告)日：2005-08-18

申请号：US11006076

申请日：2004-12-06

申请人： Edward Perkins ,  Carl Perez ,  Michael Lindenbaum ,  Amy Greene ,  Josephine Leung ,  Elena Fleming ,  Sandra Stewart ,  Joan Shellard

发明人： Edward Perkins ,  Carl Perez ,  Michael Lindenbaum ,  Amy Greene ,  Josephine Leung ,  Elena Fleming ,  Sandra Stewart ,  Joan Shellard

IPC分类号： A01K67/027 ,  A61K31/7088 ,  A61K48/00 ,  A61P35/00 ,  C07K14/005 ,  C07K19/00 ,  C12N5/10 ,  C12N9/22 ,  C12N15/09 ,  C12N15/63 ,  C12N15/85 ,  C12N15/90 ,  C12Q1/02 ,  C12Q1/68 ,  G01N33/15 ,  G01N33/50 ,  G01N33/58 ,  G01N33/68 ,  C12N15/74

CPC分类号： C07K14/005 ,  C12N9/22 ,  C12N15/63 ,  C12N15/85 ,  C12N15/90 ,  C12N15/902 ,  C12N2795/10322 ,  C12N2800/108 ,  C12N2800/208 ,  C12N2800/30 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/40 ,  C12N2830/60 ,  C12N2830/85 ,  C12N2840/20 ,  C12N2840/203 ,  C12Q1/6897 ,  G01N2500/10

摘要： Artificial chromosomes, including ACes, that have been engineered to contain available sites for site-specific, recombination-directed integration of DNA of interest are provided. These artificial chromosomes permit tractable, efficient, rational engineering of the chromosome for a variety of applications.

摘要翻译： 提供了人造染色体，包括ACes，其被设计成包含可用的位点，用于目标DNA的重组导向整合。 这些人造染色体允许针对各种应用的染色体的易处理，有效，合理的工程。

公开(公告)号：US20030119104A1

公开(公告)日：2003-06-26

申请号：US10161403

申请日：2002-05-30

发明人： Edward Perkins ,  Carl PErez ,  Michael Lindenbaum ,  Amy Greene ,  Josephine Leung ,  Elena Fleming ,  Sandra Stewart ,  Joan Shellard

IPC分类号： C12P021/02 ,  C12N005/06 ,  C07H021/04 ,  C12N005/04

CPC分类号： C07K14/005 ,  C12N9/22 ,  C12N15/63 ,  C12N15/85 ,  C12N15/90 ,  C12N15/902 ,  C12N2795/10322 ,  C12N2800/108 ,  C12N2800/208 ,  C12N2800/30 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/40 ,  C12N2830/60 ,  C12N2830/85 ,  C12N2840/20 ,  C12N2840/203 ,  C12Q1/6897 ,  G01N2500/10

摘要： Artificial chromosomes, including ACes, that have been engineered to contain available sites for site-specific, recombination-directed integration of DNA of interest are provided. These artificial chromosomes permit tractable, efficient, rational engineering of the chromosome for a variety of applications.