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公开(公告)号：US20110207221A1
公开(公告)日：2011-08-25
申请号：US12931760
申请日：2011-02-09
申请人： Gregory J. Cost , Dmitry M. Guschin , Fyodor Urnov
发明人： Gregory J. Cost , Dmitry M. Guschin , Fyodor Urnov
CPC分类号： C12N15/902 , C07K2319/80 , C07K2319/81 , C12N9/22 , C12N15/907
摘要： Disclosed herein are donor molecules comprising single-stranded complementary regions flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.
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公开(公告)号：US09255259B2
公开(公告)日：2016-02-09
申请号：US12931760
申请日：2011-02-09
申请人： Gregory J. Cost , Dmitry M. Guschin , Fyodor Urnov
发明人： Gregory J. Cost , Dmitry M. Guschin , Fyodor Urnov
CPC分类号： C12N15/902 , C07K2319/80 , C07K2319/81 , C12N9/22 , C12N15/907
摘要： Disclosed herein are donor molecules comprising single-stranded complementary regions flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.
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3.发明授权公开(公告)号：US07919313B2
公开(公告)日：2011-04-05
申请号：US12218035
申请日：2008-07-10
CPC分类号： C12N15/907 , C07K14/4703 , C07K16/00 , C12N9/1051 , C12N15/85 , C12N15/87 , C12N2800/80 , C12P21/00
摘要： Disclosed herein are methods and compositions for inactivating a FUT8 gene, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
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公开(公告)号：US07914796B2
公开(公告)日：2011-03-29
申请号：US12217185
申请日：2008-07-02
CPC分类号： C12N9/22
摘要： Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising these polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence.
摘要翻译： 本文公开了工程化裂解半结构域; 包含这些工程化裂解半结构域的融合多肽; 编码工程化切割半结构域和融合蛋白的多核苷酸; 和包含这些多核苷酸和/或融合蛋白的细胞。 还描述了使用这些多肽和多核苷酸的方法，例如用于基因组序列的靶向切割。
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5.发明授权Methods and compositions for inactivating alpha 1,6 fucosyltransferase (FUT8) gene expression 有权用于灭活α16岩藻糖基转移酶（FUT8）基因表达的方法和组合物公开(公告)号：US08772025B2
公开(公告)日：2014-07-08
申请号：US12931265
申请日：2011-01-27
CPC分类号： C12N15/907 , C07K14/4703 , C07K16/00 , C12N9/1051 , C12N15/85 , C12N15/87 , C12N2800/80 , C12P21/00
摘要： Disclosed herein are methods and compositions for inactivating a FUT8 gene, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
摘要翻译： 本文公开了使用包含锌指蛋白和切割结构域或裂解半结构域的融合蛋白来灭活FUT8基因的方法和组合物。 还提供了编码所述融合蛋白的多核苷酸，以及包含所述多核苷酸和融合蛋白的细胞。
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6.发明授权公开(公告)号：US09005973B2
公开(公告)日：2015-04-14
申请号：US13135558
申请日：2011-07-08
申请人： Gregory J. Cost , Dmitry M. Guschin , Fyodor Urnov
发明人： Gregory J. Cost , Dmitry M. Guschin , Fyodor Urnov
CPC分类号： C12N15/902 , C07K2319/80 , C07K2319/81 , C12N9/22 , C12N15/907
摘要： Disclosed herein are donor molecules comprising single-stranded complementary regions flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.
摘要翻译： 本文公开了包含一个或多个感兴趣序列侧翼的单链互补区的供体分子。 包含这些分子的供体分子和/或组合物可用于将外源序列靶向整合到细胞基因组中的特定感兴趣区域的方法中。
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7.发明申请公开(公告)号：US20110287545A1
公开(公告)日：2011-11-24
申请号：US13135558
申请日：2011-07-08
申请人： Gregory J. Cost , Dmitry M. Guschin , Fyodor Urnov
发明人： Gregory J. Cost , Dmitry M. Guschin , Fyodor Urnov
IPC分类号： C12N15/87
CPC分类号： C12N15/902 , C07K2319/80 , C07K2319/81 , C12N9/22 , C12N15/907
摘要： Disclosed herein are donor molecules comprising single-stranded complementary regions flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.
摘要翻译： 本文公开了包含一个或多个感兴趣序列侧翼的单链互补区的供体分子。 包含这些分子的供体分子和/或组合物可用于将外源序列靶向整合到细胞基因组中的特定感兴趣区域的方法中。
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8.发明申请Methods and compositions for inactivating alpha 1,6 fucosyltransferase (FUT8) gene expression 有权用于灭活α16岩藻糖基转移酶（FUT8）基因表达的方法和组合物公开(公告)号：US20110159541A1
公开(公告)日：2011-06-30
申请号：US12931265
申请日：2011-01-27
CPC分类号： C12N15/907 , C07K14/4703 , C07K16/00 , C12N9/1051 , C12N15/85 , C12N15/87 , C12N2800/80 , C12P21/00
摘要： Disclosed herein are methods and compositions for inactivating a FUT8 gene, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
摘要翻译： 本文公开了使用包含锌指蛋白和切割结构域或裂解半结构域的融合蛋白来灭活FUT8基因的方法和组合物。 还提供了编码所述融合蛋白的多核苷酸，以及包含所述多核苷酸和融合蛋白的细胞。
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9.发明申请Methods and compositions for inactivating alpha 1,6 fucosyltransferase (FUT8) gene expression 有权用于灭活α16岩藻糖基转移酶（FUT8）基因表达的方法和组合物公开(公告)号：US20090042250A1
公开(公告)日：2009-02-12
申请号：US12218035
申请日：2008-07-10
CPC分类号： C12N15/907 , C07K14/4703 , C07K16/00 , C12N9/1051 , C12N15/85 , C12N15/87 , C12N2800/80 , C12P21/00
摘要： Disclosed herein are methods and compositions for inactivating a FUT8 gene, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
摘要翻译： 本文公开了使用包含锌指蛋白和切割结构域或裂解半结构域的融合蛋白来灭活FUT8基因的方法和组合物。 还提供了编码所述融合蛋白的多核苷酸，以及包含所述多核苷酸和融合蛋白的细胞。
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