公开(公告)号：US20170369836A1

公开(公告)日：2017-12-28

申请号：US15619515

申请日：2017-06-11

申请人： Zhaoqing Dahuanong Biological Medicine Co., Ltd ,  East China University of Science and Technology ,  Guangdong Wens Dahuanong Biotechnology Co., Ltd.

发明人： RUIAI CHEN ,  Hanzhang Lai ,  Wensong Tan ,  Xuanzi Zhan ,  Xuping Liu ,  Kangcong MAI ,  Weilan Pan ,  Xiaofen Wang ,  Huajian Chen ,  Peijun Chen ,  Jiahua Xu ,  Qin Tang ,  Wenyan Zhang ,  Yan Gao

IPC分类号： C12N5/00

CPC分类号： C12N5/00 ,  C12N5/0037 ,  C12N2500/05 ,  C12N2500/12 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/20 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/40 ,  C12N2500/76 ,  C12N2500/90

摘要： The present invention discloses a serum-free medium for full suspension culture of MDCK cells and a preparation method of the serum-free medium. The serum-free medium for full suspension culture of the MDCK cells comprises basic metabolic nutrients, nucleotide, vitamins, inorganic salts, a shear force protective agent, a cell clustering resisting agent, a pH buffer agent, a pH indicator, an influenza virus proliferation accelerant and other additives. The preparation method of the serum-free medium for the full suspension culture of the MDCK cells comprises the following steps: 1) preparing a mixed solution: dissolving and mixing raw materials; and 2) regulating pH: regulating the pH of the mixed solution to 6.3 to 6.7, and setting a constant volume to obtain the serum-free medium for the full suspension culture of the MDCK cells. The medium supports the high-density full-suspension culture of the MDCK single cells, greatly shortens a time for educating the MDCK cells from adherent cells into the serum-free full suspension cells, and is applicable to the mass production of biological products, and particularly veterinary biological products.