-
1.发明授权
公开(公告)号：US5242818A
公开(公告)日：1993-09-07
申请号：US893735
申请日：1992-06-05
IPC分类号： C12N9/12
CPC分类号： C12N9/1252
摘要： A DNA polymerase with excellent thermostability is provided. The DNA polymerase retains at least about 60% of its activity when placed at pH 8.0 for 2 hours at 85.degree. C.
摘要翻译： 提供了具有优异热稳定性的DNA聚合酶。 当在85℃下放置在pH8.0下2小时时，DNA聚合酶保留至少约60％的活性。
-
2.发明授权Thermostable DNA polymerase thermus thermophilus and a method of producing the same 失效热稳定DNA聚合酶热稳定剂及其生产方法
公开(公告)号：US5192674A
公开(公告)日：1993-03-09
申请号：US398178
申请日：1989-08-24
CPC分类号： C12N9/1252
摘要： A DNA polymerase with excellent thermostability is provided. The DNA polymerase retains at least about 60% of its activity when placed at pH 8.0 for 2 hours at 85.degree. C.
-
3.发明授权
公开(公告)号：US4647532A
公开(公告)日：1987-03-03
申请号：US714743
申请日：1985-03-22
CPC分类号： G01N33/52 , C12Q1/28 , G01N33/535 , Y10T436/206664
摘要： A method for determination of hydrogen peroxide by chemiluminescence analysis which consists of reacting a sample containing hydrogen peroxide with an oxidizable non-fluorescent substance in the presence of an oxidizing catalyst to convert the non-fluorescent substance to a fluorescent substance, reacting the fluorescent substance with an oxalic acid di-ester and hydrogen peroxide in the presence of an inhibitor which inactivates the oxidizing catalyst, and then measuring the amount of light emission produced to determine the amount of hydrogen peroxide contained in the sample. Determination and enzyme immunoassay for a component other than hydrogen peroxide in a sample from living body and evaluation of bactericidal activity in phagocytosis of leukocytes by utilizing this method are also disclosed.
摘要翻译： 一种通过化学发光分析测定过氧化氢的方法，该方法包括在氧化催化剂存在下使含有过氧化氢的样品与可氧化非荧光物质反应，将非荧光物质转化为荧光物质，使荧光物质与 草酸二酯和过氧化氢，在抑制剂存在下使氧化催化剂失活，然后测量产生的发光量以确定样品中所含的过氧化氢的量。 还公开了来自活体样品中除过氧化氢以外的成分的测定和酶免疫测定，以及利用该方法评价白细胞吞噬活性的杀菌活性。
-
公开(公告)号：US5413926A
公开(公告)日：1995-05-09
申请号：US894085
申请日：1992-06-05
CPC分类号： C12N9/1252
摘要： A DNA polymerase from Thermus thermophilus which retains at least 60% of its activity when placed at pH 8.0 and 85.degree. C. for 2 hours is disclosed. The DNA polymerase has a pH optimum of about 8.0, a temperature optimum of about 75.degree. C., a mass of about 85 to 95 kDa and an optimal saline concentration of about 20-60 mM NaCl.
摘要翻译： 公开了嗜热嗜热栖热菌的DNA聚合酶，其在pH 8.0和85℃放置2小时时保留其活性的至少60％。 DNA聚合酶具有约8.0的pH最佳值，约75℃的最适温度，约85至95kDa的质量和约20-60mM NaCl的最佳盐浓度。
-
5.发明授权Element for assaying rheumatoid factor quantitatively and method of assaying the same 失效用于定量测定湿度因子的元件及其测定方法
公开(公告)号：US5124250A
公开(公告)日：1992-06-23
申请号：US415237
申请日：1989-09-11
申请人： Mami Inada , Hakuji Matsumoto , Tsuneo Hanyu , Kyoichi Kano
发明人： Mami Inada , Hakuji Matsumoto , Tsuneo Hanyu , Kyoichi Kano
IPC分类号： G01N33/545 , G01N33/564
CPC分类号： G01N33/564 , G01N33/545 , G01N2800/102 , Y10S435/97 , Y10S436/811
摘要： An element for assaying a rheumatoid factor quantitatively in biosamples, comprising a polyalkyl methacrylate solid carrier and serum albumin immobilized thereon, the serum albumin being immunologically bound with anti-albumin rabbit IgG, and a method of assaying a rheumatoid factor quantitatively by immunoglobulin class in which the element is reacted with the biosample, then the element-bound rheumatoid factor is reacted with enzyme-labeled anti-human IgG, enzyme-labeled anti-human IgM or enzyme-labeled anti-human IgA, and then the amount of the marker enzyme is determined.
摘要翻译： PCT No.PCT / JP89 / 00024 Sec。 371日期1989年9月11日 102（e）1989年9月11日PCT PCT 1991年1月11日PCT。 公开号WO89 / 06800 日期：1989年7月27日。一种用于在生物样品中定量测定类风湿因子的元件，其包含固定在其上的甲基丙烯酸烷基酯固体载体和血清白蛋白，血清白蛋白与抗白蛋白兔IgG免疫结合，以及测定类风湿 其中元件与生物样品反应的免疫球蛋白级别定量定量，然后将元件结合的类风湿因子与酶标记的抗人IgG，酶标记的抗人IgM或酶标记的抗人IgA反应，以及 然后确定标记酶的量。
-
-
-
-
搜索结果
5 条记录 (耗时 0.009 秒)
