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    Production method for PvuI restriction endonuclease
    5.
    发明授权
    Production method for PvuI restriction endonuclease 失效
    PvuI限制性内切核酸酶的生产方法

    公开(公告)号：US5049501A

    公开(公告)日：1991-09-17

    申请号：US451201

    申请日：1989-12-14

    申请人： Nobuhiro Katsuragi ,  Bunsei Kawakami ,  Yoshihiko Maekawa

    发明人： Nobuhiro Katsuragi ,  Bunsei Kawakami ,  Yoshihiko Maekawa

    IPC分类号： C12N9/16 ,  C12N1/21 ,  C12N9/22 ,  C12N15/09 ,  C12N15/55 ,  C12R1/19 ,  C12R1/37

    CPC分类号： C12N9/22

    摘要： A recombinant vector comprising an incorporated chromosome DNA fragment containing a PvuI restriction enconuclease gene derived from Proteus vulgaris, a host transformed with the recombinant vector and a method of producing PvuI restriction endonuclease characterized in that the transformed host is cultivated and PvuI restriction endonuclease is harvested from the resulting culture. Since the host transformed with the recombinant vector of the present invention produces PvuI alone, it is unnecessary to remove PvuII in the purification process for PvuI, and further, since its productivity for nonspecific DNase is lower in comparison with conventional producer bacteria, DNase can be removed easily, making the production of PvuI easy.

    Game program for moving object from one display to another display
    6.
    发明授权
    Game program for moving object from one display to another display 有权
    将对象从一个显示器移动到另一个显示器的游戏程序

    公开(公告)号：US08602889B2

    公开(公告)日：2013-12-10

    申请号：US11542240

    申请日：2006-10-04

    申请人： Yoshihiko Maekawa ,  Hiroyuki Kubota

    发明人： Yoshihiko Maekawa ,  Hiroyuki Kubota

    IPC分类号： A63F9/00

    CPC分类号： A63F13/26 ,  A63F13/00 ,  A63F2300/204 ,  A63F2300/301 ,  A63F2300/305

    摘要： When a method of operating a player character 40 is displayed on a first LCD 11, a movement of an enemy character 41 displayed on a second LCD 12 temporarily stops and the player character 40 moves from a display screen of the second LCD 12 to a display screen of the first LCD 11. Thus, a player's sight line is guided from the display screen of the second LCD 12 to the display screen of the first LCD 11 along with the movement of the player character 40, thereby allowing the player to confirm; with no oversight, that explanatory information regarding the method of operating the player character 40 is displayed.

    摘要翻译： 当在第一LCD11上显示操作玩家角色40的方法时，显示在第二LCD 12上的敌人角色41的移动临时停止，并且玩家角色40从第二LCD 12的显示屏移动到显示器 因此，随着玩家角色40的移动，玩家的视线从第二LCD 12的显示屏被引导到第一LCD11的显示屏幕，从而允许玩家确认; 没有监视，显示关于操作玩家角色40的方法的说明信息。

    Game program
    7.
    发明申请
    Game program 有权
    游戏程序

    公开(公告)号：US20070078007A1

    公开(公告)日：2007-04-05

    申请号：US11542240

    申请日：2006-10-04

    申请人： Yoshihiko Maekawa ,  Hiroyuki Kubota

    发明人： Yoshihiko Maekawa ,  Hiroyuki Kubota

    IPC分类号： A63F13/00

    CPC分类号： A63F13/26 ,  A63F13/00 ,  A63F2300/204 ,  A63F2300/301 ,  A63F2300/305

    摘要： When a method of operating a player character 40 is displayed on a first LCD 11, a movement of an enemy character 41 displayed on a second LCD 12 temporarily stops and the player character 40 moves from a display screen of the second LCD 12 to a display screen of the first LCD 11. Thus, a player's sight line is guided from the display screen of the second LCD 12 to the display screen of the first LCD 11 along with the movement of the player character 40, thereby allowing the player to confirm; with no oversight, that explanatory information regarding the method of operating the player character 40 is displayed.

    摘要翻译： 当在第一LCD11上显示操作玩家角色40的方法时，显示在第二LCD 12上的敌人角色41的移动临时停止，并且玩家角色40从第二LCD 12的显示屏移动到显示器 因此，随着玩家角色40的移动，玩家的视线从第二LCD 12的显示屏被引导到第一LCD11的显示屏幕，从而允许玩家确认; 没有监视，显示关于操作玩家角色40的方法的说明信息。

    Method for producing glycoconjugates
    8.
    发明授权
    Method for producing glycoconjugates 失效
    糖缀合物的制备方法

    公开(公告)号：US6046040A

    公开(公告)日：2000-04-04

    申请号：US123766

    申请日：1998-07-28

    申请人： Susumu Nishiguchi ,  Yoshihiko Maekawa ,  Shin-ichiro Nishimura ,  Kuriko Yamada

    发明人： Susumu Nishiguchi ,  Yoshihiko Maekawa ,  Shin-ichiro Nishimura ,  Kuriko Yamada

    IPC分类号： C12P19/44 ,  C12N9/10 ,  C12N11/10 ,  C12P19/18 ,  C12P7/64 ,  C12P19/00 ,  C12P19/14 ,  C12P19/26

    CPC分类号： C12N9/1048 ,  C12N11/10 ,  C12P19/18

    摘要： A method for producing glycoconjugate, which comprises the steps of:(i) binding a sugar residue to the side chain of a water-soluble polymer via a linker having a selectively cleavable linkage to give a primer, and bringing said primer into contact with an immobilized glycosyltransferase in the presence of a sugar nucleotide, to transfer a sugar residue of said sugar nucleotide to the sugar residue of said primer,(ii) elongating a sugar chain by transfer of plural sugar residues by repeating the step (i) at least once,(iii) removing, where necessary, a by-produced nucleotide or an unreacted sugar nucleotide, and(iv) repeating the steps (i)-(iii) where necessary and releasing the glycoconjugate by selectively cleaving the cleavable linkage in the linker, from the above-mentioned primer connecting the sugar chain elongated by the transfer of plural sugar residues,and a method for producing a sphingoglycolipid.

    摘要翻译： 一种制备糖缀合物的方法，其包括以下步骤：（i）通过具有选择性切割键的接头将糖残基与水溶性聚合物的侧链结合，得到底漆，并使所述底漆与 在糖核苷酸存在下固定糖基转移酶，将所述糖核苷酸的糖残基转移到所述引物的糖残基，（ii）通过重复步骤（i）至少一次转移多个糖残基来延长糖链 ，（iii）在必要时除去副产物核苷酸或未反应的糖核苷酸，和（iv）在必要时重复步骤（i） - （iii），通过选择性地切割接头中的可切割连接键释放糖缀合物， 通过连接通过多个糖残基的转移而延伸的糖链的上述引物和用于生产鞘糖脂的方法。

    Production of cotton fiber with improved fiber characteristics
    9.
    发明授权
    Production of cotton fiber with improved fiber characteristics 失效
    生产具有纤维特性提高的棉纤维

    公开(公告)号：US06563021B1

    公开(公告)日：2003-05-13

    申请号：US09760892

    申请日：2001-01-17

    申请人： Yoshihisa Kasukabe ,  Koichi Fujisawa ,  Susumu Nishiguchi ,  Yoshihiko Maekawa ,  Randy Dale Allen

    发明人： Yoshihisa Kasukabe ,  Koichi Fujisawa ,  Susumu Nishiguchi ,  Yoshihiko Maekawa ,  Randy Dale Allen

    IPC分类号： A01H100

    CPC分类号： C12N15/8261 ,  A01N49/00 ,  C07K14/415 ,  C12N15/8242 ,  Y02A40/146

    摘要： Methods are disclosed to achieve an improvement in the characteristics and yield of cotton fibers. In one method, a cotton plant of the genus Gossypium in seed form or in growth stage is treated with a brassinosteroid. In another method, an ovule culture is prepared from a cotton plant of the genus Gossypium in a brassinosteroid-containing liquid medium. Cotton fibers with improved fiber characteristics are obtained from the cotton bolls of the treated plant or from the cultured ovules. Also disclosed are a method for inducing specific genes expression in a cotton plant to produce cotton fibers with improved fiber characteristics by treatment with a brassinosteroid, and a cotton plant produced by this method; as well as a novel gene capable of changing the degree of its expression found in the ovules of a cotton plant treated with a brassinosteroid, a gene capable of hybridizing with this novel gene, a recombinant plasmid containing this novel gene, a transformant containing this recombinant plasmid, and the like.

    摘要翻译： 公开了实现棉纤维特性和产量改进的方法。 在一种方法中，种子形式或生长阶段的棉属棉花植物用油菜素固醇处理。 在另一种方法中，在含油菜素固醇的液体培养基中从棉属棉花植物制备胚珠培养物。 具有改善的纤维特性的棉纤维由处理植物的棉铃或培养的胚珠获得。 还公开了一种在棉花植物中诱导特异性基因表达的方法，以通过用油菜素甾醇处理生产具有改善的纤维特性的棉纤维和通过该方法生产的棉花植物; 以及能够改变用油菜素类固醇处理的棉花植物的胚珠中发现的表达程度的新型基因，能够与该新基因杂交的基因，含有该新基因的重组质粒，含有该重组体的转化体 质粒等。

    Production of cotton fibers with improved fiber characteristics by treatment with brassinosteroids
    10.
    发明授权
    Production of cotton fibers with improved fiber characteristics by treatment with brassinosteroids 失效
    通过处理油菜素类固醇生产具有改善纤维特性的棉纤维

    公开(公告)号：US5880110A

    公开(公告)日：1999-03-09

    申请号：US391696

    申请日：1995-02-21

    申请人： Yoshihisa Kasukabe ,  Koichi Fujisawa ,  Susumu Nishiguchi ,  Yoshihiko Maekawa ,  Randy Dale Allen

    发明人： Yoshihisa Kasukabe ,  Koichi Fujisawa ,  Susumu Nishiguchi ,  Yoshihiko Maekawa ,  Randy Dale Allen

    IPC分类号： A01H1/00 ,  A01H3/04 ,  A01N49/00 ,  C07J73/00 ,  C07K14/415 ,  C12N1/21 ,  C12N5/04 ,  C12N5/10 ,  C12N15/00 ,  C12N15/01 ,  C12N15/09 ,  C12N15/29 ,  C12N15/82 ,  C12R1/19 ,  A01N43/04

    CPC分类号： C12N15/8261 ,  A01N49/00 ,  C07K14/415 ,  C12N15/8242

    摘要： Methods are disclosed to achieve an improvement in the characteristics and yield of cotton fibers. In one method, a cotton plant of the genus Gossypium in seed form or in growth stage is treated with a brassinosteroid. In another method, an ovule culture is prepared from a cotton plant of the genus Gossypium in a brassinosteroid-containing liquid medium. Cotton fibers with improved fiber characteristics are obtained from the cotton bolls of the treated plant or from the cultured ovules. Also disclosed are a method for inducing specific genes expression in a cotton plant to produce cotton fibers with improved fiber characteristics by treatment with a brassinosteroid, and a cotton plant produced by this method; as well as a novel gene capable of changing the degree of its expression found in the ovules of a cotton plant treated with a brassinosteroid, a gene capable of hybridizing with this novel gene, a recombinant plasmid containing this novel gene, a transformant containing this recombinant plasmid, and the like.

    摘要翻译： 公开了实现棉纤维特性和产量改进的方法。 在一种方法中，种子形式或生长阶段的棉属棉花植物用油菜素固醇处理。 在另一种方法中，在含油菜素固醇的液体培养基中从棉属棉花植物制备胚珠培养物。 具有改善的纤维特性的棉纤维由处理植物的棉铃或培养的胚珠获得。 还公开了一种在棉花植物中诱导特异性基因表达的方法，以通过用油菜素甾醇处理生产具有改善的纤维特性的棉纤维和通过该方法生产的棉花植物; 以及能够改变用油菜素甾醇处理的棉花植物的胚珠中发现的表达程度的新型基因，能够与该新基因杂交的基因，含有该新基因的重组质粒，含有该重组体的转化体 质粒等。