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    Proinsulin derivative and process for producing human insulin
    2.
    发明授权
    Proinsulin derivative and process for producing human insulin 失效
    胰岛素原衍生物及其制备人胰岛素的方法

    公开(公告)号：US5962267A

    公开(公告)日：1999-10-05

    申请号：US600783

    申请日：1996-02-13

    申请人： Hang-Cheol Shin ,  Seung-Gu Chang ,  Dae-Young Kim ,  Chong-Suhl Kim

    发明人： Hang-Cheol Shin ,  Seung-Gu Chang ,  Dae-Young Kim ,  Chong-Suhl Kim

    IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K38/28 ,  C07H21/04 ,  C07K14/575 ,  C07K14/62 ,  C12N1/21 ,  C12N15/17 ,  C12P21/02 ,  C12R1/19 ,  C12N15/63

    CPC分类号： C07K14/62 ,  A61K38/00 ,  C07K2319/00

    摘要： A process for preparing human insulin, which comprises the steps of: preparing a DNA encoding a human proinsulin derivative having the following formula (I); inserting the DNA into a vector to construct an expression vector for the expression of the human proinsulin derivative; transforming a host cell with the expression vector; culturing the resulting transformant under a condition that allows the expression of the human proinsulin derivative; purifying human proinsulin derivative from the culture; and preparing human insulin from the human proinsulin derivative by an enzymatic hydrolysis:B chain-Z-A chain (I)Wherein,said A and B chains are respectively human insulin chains;Z is a peptide of formula U-U-X-P-J-(X')n-U-U;U is an arginine or lysin residue;X and X' are independently any amino acid residue;P is a proline residue;J is a glycine, arginine or lysine residue; andn is 0 or an integer of 1 to 5.

    摘要翻译： 一种制备人胰岛素的方法，其包括以下步骤：制备编码具有下式（I）的人胰岛素原衍生物的DNA; 将DNA插入载体以构建用于表达人胰岛素原衍生物的表达载体; 用表达载体转化宿主细胞; 在允许人胰岛素原衍生物表达的条件下培养所得转化体; 从培养物中纯化人胰岛素原衍生物; 并通过酶水解从人胰岛素原衍生物制备人胰岛素：B链-Z-A链（I）其中所述A和B链分别是人胰岛素链; Z是式U-U-X-P-J-（X'）n-U-U的肽; U是精氨酸或赖氨酸残基; X和X'独立地是任何氨基酸残基; P是脯氨酸残基; J是甘氨酸，精氨酸或赖氨酸残基; n为0或1〜5的整数。

    METHOD FOR PREPARING SOLUBLE AND ACTIVE RECOMBINANT PROTEINS USING PDI AS A FUSION PARTNER
    4.
    发明申请
    METHOD FOR PREPARING SOLUBLE AND ACTIVE RECOMBINANT PROTEINS USING PDI AS A FUSION PARTNER 有权
    使用PDI作为融合合作伙伴制备可溶性和活性重组蛋白的方法

    公开(公告)号：US20090305351A1

    公开(公告)日：2009-12-10

    申请号：US12091291

    申请日：2005-10-24

    申请人： Hang-Cheol Shin ,  Yean-Hee Park ,  Hyang-Do Song ,  Eung-Yoon Kim ,  Ha-A-Rin Chon ,  Hye-Ran Hyun

    发明人： Hang-Cheol Shin ,  Yean-Hee Park ,  Hyang-Do Song ,  Eung-Yoon Kim ,  Ha-A-Rin Chon ,  Hye-Ran Hyun

    IPC分类号： C12P21/06 ,  C12N9/90 ,  C12N1/21 ,  C12N15/63 ,  C07H21/04

    CPC分类号： C12N15/62 ,  C07K2319/00 ,  C07K2319/21 ,  C12N9/90 ,  C12P21/02

    摘要： The present invention relates to a method for producing a recombinant protein capable of increasing expression rate of a target protein and also improving solubility and folding of the expressed target protein using a modified protein disulfide isomerase (PDI) as a fusion partner, and an expression vector containing the modified PDI gene as a fusion partner. The method for preparing a recombinant protein using a modified PDI as a fusion partner according to the present invention may solve the problems concerning a low yield and solubility and folding that conventional fusion partners have, and be widely used for protein drug and industrial protein production.

    摘要翻译： 本发明涉及能够提高靶蛋白表达率的重组蛋白质的制造方法，并且使用修饰的蛋白质二硫键异构酶（PDI）作为融合对象，提高表达的靶蛋白的溶解性和折叠性，以及表达载体 含有修饰的PDI基因作为融合配偶体。 使用修饰的PDI作为本发明的融合对象的重组蛋白质的制备方法可以解决常规的融合对象具有的低产率和溶解性以及折叠的问题，并被广泛用于蛋白质药物和工业蛋白质的生产。

    Method for preparing soluble and active recombinant proteins usins PDI as a fusion partner
    6.
    发明授权
    Method for preparing soluble and active recombinant proteins usins PDI as a fusion partner 有权
    制备可溶性和活性重组蛋白的方法使PDI作为融合配偶体

    公开(公告)号：US08080387B2

    公开(公告)日：2011-12-20

    申请号：US12091291

    申请日：2005-10-24

    申请人： Hang-Cheol Shin ,  Yean-Hee Park ,  Hyang-Do Song ,  Eung-Yoon Kim ,  Ha-A-Rin Chon ,  Hye-Ran Hyun

    发明人： Hang-Cheol Shin ,  Yean-Hee Park ,  Hyang-Do Song ,  Eung-Yoon Kim ,  Ha-A-Rin Chon ,  Hye-Ran Hyun

    IPC分类号： C12N15/01 ,  A61K38/00

    CPC分类号： C12N15/62 ,  C07K2319/00 ,  C07K2319/21 ,  C12N9/90 ,  C12P21/02

    摘要： The present invention relates to a method for producing a recombinant protein capable of increasing expression rate of a target protein and also improving solubility and folding of the expressed target protein using a modified protein disulfide isomerase (PDI) as a fusion partner, and an expression vector containing the modified PDI gene as a fusion partner. The method for preparing a recombinant protein using a modified PDI as a fusion partner according to the present invention may solve the problems concerning a low yield and solubility and folding that conventional fusion partners have, and be widely used for protein drug and industrial protein production.

    摘要翻译： 本发明涉及能够提高靶蛋白表达率的重组蛋白质的制造方法，并且使用修饰的蛋白质二硫键异构酶（PDI）作为融合对象，提高表达的靶蛋白的溶解性和折叠性，以及表达载体 含有修饰的PDI基因作为融合配偶体。 使用修饰的PDI作为本发明的融合对象的重组蛋白质的制备方法可以解决常规的融合对象具有的低产率和溶解性以及折叠的问题，并被广泛用于蛋白质药物和工业蛋白质的生产。

    Method for improving solubility and folding efficiency of target proteins using RNA as molecular chaperone
    7.
    发明授权
    Method for improving solubility and folding efficiency of target proteins using RNA as molecular chaperone 有权
    使用RNA作为分子伴侣来提高靶蛋白的溶解度和折叠效率的方法

    公开(公告)号：US07759303B2

    公开(公告)日：2010-07-20

    申请号：US11482401

    申请日：2006-07-07

    申请人： Baik Lin Seong ,  Seong-Il Choi ,  Hang Cheol Shin ,  Ki-Sun Ryu ,  Kyoung Sim Han ,  Chul Woo Kim ,  Byung Hee Kim

    发明人： Baik Lin Seong ,  Seong-Il Choi ,  Hang Cheol Shin ,  Ki-Sun Ryu ,  Kyoung Sim Han ,  Chul Woo Kim ,  Byung Hee Kim

    IPC分类号： C07K14/00 ,  C07H21/02 ,  C12N15/00

    CPC分类号： C07H21/04 ,  C07H21/02 ,  C07K2319/35 ,  C07K2319/85 ,  C12N15/62 ,  C12P21/02

    摘要： Disclosed is a method for improving folding efficiency and solubility of a target protein linked to a RNA-binding protein by using RNA molecule as a molecular chaperone, wherein the RNA molecule interacts with the RNA-binding protein. More particularly, the present invention discloses method for improving folding efficiency and solubility of a target protein by transformation of a host cell with a expression vector comprising a polynucleotide encoding the target protein linked to an RNA-binding protein; culturing the transformed host cell in an appropriate culture medium under the condition that an RNA molecule either resident inside the host cell or provided by cotransformation of the host cell with polynucleotide encoding the RNA molecule interacts with the RNA-binding protein; and purifying the soluble protein from host cell lysate. The method of the present invention is very useful for production of soluble proteins for therapeutic, prophylactic and diagnostic applications.

    摘要翻译： 公开了通过使用RNA分子作为分子伴侣来提高与RNA结合蛋白质连接的靶蛋白的折叠效率和溶解度的方法，其中RNA分子与RNA结合蛋白相互作用。 更具体地，本发明公开了通过用包含编码与RNA结合蛋白连接的靶蛋白的多核苷酸的表达载体转化宿主细胞来提高靶蛋白的折叠效率和溶解度的方法。 在宿主在宿主细胞内的RNA分子或通过宿主细胞与编码RNA分子的多核苷酸共转化的RNA分子与RNA结合蛋白相互作用的条件下，在适当的培养基中培养转化的宿主细胞; 并从宿主细胞裂解液纯化可溶性蛋白质。 本发明的方法对于用于治疗，预防和诊断应用的可溶性蛋白质的生产非常有用。