Inducible fluorescence assay
    4.
    发明授权
    Inducible fluorescence assay 有权
    诱导荧光测定

    公开(公告)号:US07635598B2

    公开(公告)日:2009-12-22

    申请号:US11177998

    申请日:2005-07-08

    摘要: In one aspect, the invention provides fluorescent probes and assays. The probes include a fluorophore-quencher pair that undergoes a switch from dark to fluorescent in response to a reaction of the quencher. The switch of the probe from dark to fluorescent is typically mediated by an enzyme that acts directly or indirectly on the quencher, interfering with its ability to quench fluorescence emission from the fluorophore.In another aspect, the invention provides a reporter gene assay system and methods of using this system. The assay system includes a fluorophore-quencher probe and an enzyme that acts directly or indirectly on the quencher, increasing the fluorescent emission of the fluorophore. In still other aspects, the invention provides nucleic acid constructs and cells expressing the peptide products of these constructs.In assays of the invention, the presence of a target substance is detected by the switching of fluorescence mediated by the change in oxidation state of the quencher. The assay systems are of use in numerous assay formats, e.g., confirming the expression of the enzyme that acts on the quencher, and/or detecting a species conjugated to the enzyme.

    摘要翻译: 一方面,本发明提供了荧光探针和测定法。 探针包括响应于猝灭剂的反应经历从暗变为荧光的荧光团 - 猝灭剂对。 探针从黑暗到荧光的切换通常由直接或间接作用于猝灭剂的酶介导,干扰其从荧光团中淬灭荧光发射的能力。 另一方面,本发明提供报道基因测定系统和使用该系统的方法。 测定系统包括荧光团猝灭剂探针和直接或间接作用于猝灭剂的酶,增加荧光团的荧光发射。 在其它方面,本发明提供了表达这些构建体的肽产物的核酸构建体和细胞。 在本发明的测定中,通过由猝灭剂的氧化态的变化介导的荧光的转换来检测目标物质的存在。 测定系统可用于许多测定形式,例如确认作用于猝灭剂的酶的表达和/或检测与酶缀合的物质。

    Process for production of paper
    5.
    发明授权
    Process for production of paper 有权
    纸张生产流程

    公开(公告)号:US07488402B2

    公开(公告)日:2009-02-10

    申请号:US11149879

    申请日:2005-06-10

    CPC分类号: D21H17/17 D21H17/24 D21H21/16

    摘要: The invention related to process for the production of paper from an aqueous suspension containing cellulosic fibers, and optional fillers, which comprises adding to the suspension (i) a cationic vinyl addition polymer prepared by polymerizing one or more ethylenically unsaturated monomers comprising a cationic monomer; (ii) an anionic polymer having one or more aromatic groups selected from the group consisting of polymers obtained by condensation of formaldehyde with benzene-based and naphthalene-based compounds, polyurethanes, polysaccharides, and lignin-based polymers; wherein (i) and (ii) are separately added to the suspension; and (iii) a low molecular weight cationic organic polymer having a molecular weight in the range of from 2,000 to about 700,000; or an anionic microparticulate material; and forming and draining the obtained suspension on a wire.

    摘要翻译: 本发明涉及从含有纤维素纤维的水悬浮液和任选的填料生产纸的方法,其包括向悬浮液中加入(i)通过聚合一种或多种包含阳离子单体的烯属不饱和单体制备的阳离子乙烯基加成聚合物; (ii)具有一个或多个芳族基团的阴离子聚合物,所述芳族基团选自甲醛与苯基和萘基化合物缩合得到的聚合物,聚氨酯,多糖和木质素基聚合物; 其中(i)和(ii)分别加入悬浮液中; 和(iii)分子量在2,000至约700,000范围内的低分子量阳离子有机聚合物; 或阴离子微粒材料; 并将得到的悬浮液在线上形成和排出。

    Process for the production of paper using silica-based sols
    6.
    发明授权
    Process for the production of paper using silica-based sols 失效
    使用二氧化硅基溶胶生产纸的方法

    公开(公告)号:US06893538B2

    公开(公告)日:2005-05-17

    申请号:US10168408

    申请日:2000-11-29

    摘要: The invention relates to an aqueous silica-based sol comprising a nitrogen-containing organic compound and silica-based particles with a specific surface area of at least 300 square meters per gram of silica. The invention further relates to a process for the production of an aqueous silica-based sol comprising a nitrogen-containing organic compound which comprises incorporating a nitrogen-containing organic compound into a silica-based sol containing silica-based particles with a specific surface area of at least 300 square meters per gram of silica. The invention also relates to the use of an aqueous silica-based sol comprising a nitrogen-containing organic compound and silica-based particles with a specific surface area of at least 300 square meters per gram of silica as a flocculating agent in the production of pulp and paper and in water purification. The invention further relates to a process for the production of paper from an aqueous suspension containing cellulosic fibres, and optional fillers, which comprises adding to the suspension (i) an aqueous silica-based sol comprising an organic nitrogen-containing compound and (ii) at least one charged organic polymer, forming and draining the suspension on a wire.

    摘要翻译: 本发明涉及包含含氮有机化合物的水性二氧化硅基溶胶和比表面积至少为300平方米/克二氧化硅的二氧化硅基颗粒。 本发明还涉及含有含氮有机化合物的含水二氧化硅基溶胶的制备方法,该方法包括将含氮有机化合物掺入含二氧化硅基颗粒的二氧化硅基溶胶中,所述二氧化硅基溶胶的比表面积为 至少300平方米每克二氧化硅。 本发明还涉及包含含氮有机化合物的水性二氧化硅基溶胶和比表面积为至少300平方米/克二氧化硅作为絮凝剂的二氧化硅基颗粒在制备纸浆中的用途 纸和水净化。 本发明还涉及从含有纤维素纤维和任选的填料的水性悬浮液生产纸的方法,其包括将悬浮液(i)包含含有机含氮化合物的二氧化硅水溶胶和(ii) 至少一个带电的有机聚合物,在线上形成和排出悬浮液。

    Papermaking
    7.
    发明授权
    Papermaking 失效
    造纸

    公开(公告)号:US4385961A

    公开(公告)日:1983-05-31

    申请号:US238645

    申请日:1981-02-26

    摘要: A papermaking process in which an aqueous papermaking stock containing a cellulosic pulp is formed and dried. The stock includes a binder comprising colloidal silicic acid, and cationic starch which is added to the stock before the sheet is formed. The manner of addition involves first adding and intermixing in the stock a portion of the colloidal silicic acid and then the cationic starch and, after an agglomerate has formed, adding and intermixing the remainder of the colloidal silicic acid prior to the formation of the sheet. The method results in improved sheet properties and improved retention of filler and fines on the wire.

    摘要翻译: 其中形成含有纤维素纸浆的水性造纸原料并干燥的造纸方法。 原料包括粘合剂,其包含胶体硅酸和阳离子淀粉,其在片形成之前添加到原料中。 添加的方式包括首先在原料中加入和混合一部分胶体硅酸,然后加入阳离子淀粉,并且在形成附聚物之后,在形成片材之前加入和混合剩余的胶态硅酸。 该方法导致改善的片材性能和改善填料和细粒在线材上的保留性。

    Silica-based sols
    8.
    发明授权
    Silica-based sols 失效
    二氧化硅溶胶

    公开(公告)号:US06379500B2

    公开(公告)日:2002-04-30

    申请号:US09741300

    申请日:2000-12-19

    IPC分类号: D21H1768

    摘要: An aqueous silica-based sol comprising a nitrogen-containing organic compound and silica-based particles with a specific surface area of at least 300 square meters per gram of silica. The invention further relates to a process for the production of an aqueous silica-based sol comprising a nitrogen-containing organic compound which comprises incorporating a nitrogen-containing organic compound into a silica-based sol containing silica-based particles with a specific surface area of at least 300 square meters per gram of silica. The invention also relates to the use of an aqueous silica-based sol comprising a nitrogen-containing organic compound and silica-based particles with a specific surface area of at least 300 square meters per gram of silica as a flocculating agent in the production of pulp and paper and in water purification. The invention further relates to a process for the production of paper from an aqueous suspension containing cellulosic fibers, and optional fillers, which comprises adding to the suspension (i) an aqueous silica-based sol comprising an organic nitrogen-containing compound and (ii) at least one charged organic polymer, forming and draining the suspension on a wire.

    摘要翻译: 包含含氮有机化合物的水性二氧化硅基溶胶和比表面积至少为300平方米/克二氧化硅的二氧化硅基颗粒。 本发明还涉及含有含氮有机化合物的含水二氧化硅基溶胶的制备方法,该方法包括将含氮有机化合物掺入含二氧化硅基颗粒的二氧化硅基溶胶中,所述二氧化硅基溶胶的比表面积为 至少300平方米每克二氧化硅。 本发明还涉及包含含氮有机化合物的水性二氧化硅基溶胶和比表面积为至少300平方米/克二氧化硅作为絮凝剂的二氧化硅基颗粒在制备纸浆中的用途 纸和水净化。 本发明还涉及从含有纤维素纤维和任选的填料的水性悬浮液生产纸的方法,其包括将悬浮液(i)包含含有机含氮化合物的二氧化硅水溶胶和(ii) 至少一个带电的有机聚合物,在线上形成和排出悬浮液。

    Process for the production of paper
    10.
    发明授权
    Process for the production of paper 有权
    生产纸张的过程

    公开(公告)号:US06918995B2

    公开(公告)日:2005-07-19

    申请号:US09923096

    申请日:2001-08-06

    CPC分类号: D21H17/17 D21H17/24 D21H21/16

    摘要: The invention relates to a process for the production of paper from an aqueous suspension containing cellulosic fibers, and optional fillers, which comprises separately adding to the suspension a cationic organic polymer having one or more aromatic groups and an anionic polymer having one or more aromatic groups, the anionic polymer being selected from step-growth polymers, polysaccharides and naturally occurring aromatic polymers and modifications thereof, forming and draining the suspension on a wire, with the proviso that if the anionic polymer is a step-growth polymer it is not an anionic melamine-sulphonic acid condensation polymer. The invention further relates to a process for the production of paper from an aqueous suspension containing cellulosic fibers, and optional fillers, which comprises separately adding to the suspension a cationic organic polymer having one or more aromatic groups and an anionic polymer having one or more aromatic groups, forming and draining the suspension on a wire, with the proviso that the anionic polymer is not an anionic polystyrene sulphonate or anionic melamine-sulphonic acid condensation polymer.

    摘要翻译: 本发明涉及从含有纤维素纤维的水性悬浮液和任选的填料生产纸的方法,其包括向悬浮液中分别加入具有一个或多个芳族基团的阳离子有机聚合物和具有一个或多个芳族基团的阴离子聚合物 ,阴离子聚合物选自步进生长聚合物,多糖和天然存在的芳族聚合物及其改性物,在悬挂线上形成和排出,条件是如果阴离子聚合物是阶梯生长聚合物,则其不是阴离子 三聚氰胺 - 磺酸缩合聚合物。 本发明还涉及从含有纤维素纤维和任选的填料的水性悬浮液生产纸的方法,其包括向悬浮液中分别加入具有一个或多个芳族基团的阳离子有机聚合物和具有一个或多个芳族化合物的阴离子聚合物 组,在线上形成和排出悬浮液,条件是阴离子聚合物不是阴离子聚苯乙烯磺酸盐或阴离子三聚氰胺 - 磺酸缩合聚合物。