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公开(公告)号：US6027886A
公开(公告)日：2000-02-22
申请号：US771781
申请日：1996-12-20
CPC分类号： C12Q1/6816
摘要： Method and kit for the quantitative detection of specific oligonucleotide or polynucleotide sequences which is characterized in that a sample mixture containing RNA or single-stranded DNA is hybridized with an oligonucleotide or polynucleotide probe(s) which are complementary to the nucleotide sequence to be determined and carry(ies) a specificplly bindable and a detectable chemical group subsequently it is admixed with an agent that cleaves single-stranded polynucleotide sequences and the immobilized or non-immobilized nucleotide probe is determined after transfer into a suitable reaction vessel. It has proven to be particularly advantageous when a mixture is used which is composed of different cleaving reagents.
摘要翻译： 用于定量检测特异性寡核苷酸或多核苷酸序列的方法和试剂盒，其特征在于含有RNA或单链DNA的样品混合物与与要测定的核苷酸序列互补的寡核苷酸或多核苷酸探针杂交， 携带特异性可结合的和可检测的化学基团，随后将其与切割单链多核苷酸序列的试剂混合，并且在转移到合适的反应容器中后测定固定的或未固定的核苷酸探针。 当使用由不同的切割试剂组成的混合物时，已经证明是特别有利的。
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公开(公告)号：US20100255469A1
公开(公告)日：2010-10-07
申请号：US12579746
申请日：2009-10-15
CPC分类号： G01N33/543
摘要： The present invention provides a method for the real time analysis of cell cultures and their molecular content. More precisely, the present invention provides a method to monitor the cellular reaction of cells to certain stimuli in real time in order to figure out a reasonable point of time to perform an analysis of the molecular content of the cells.
摘要翻译： 本发明提供了一种细胞培养物及其分子内容的实时分析方法。 更准确地说，本发明提供了一种监测细胞对实时刺激的细胞反应的方法，以便找出合理的时间点来进行细胞分子内容的分析。
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公开(公告)号：US6136531A
公开(公告)日：2000-10-24
申请号：US960153
申请日：1997-10-29
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837
摘要： The invention describes a method of quantitatively detecting specific nucleotide sequences. Said method is essentially characterized in that a single-stranded nucleic acid, particularly mRNA, which has been isolated from a mixture, e.g. a biological sample, is hybridized in solution, with a polynucleotide sequence which is essentially complementary to the sequence to be determined; the nucleic acid is then immobilized on a solid phase, and the amount of bound hybrid is determined. It has proven to be particularly advantageous if the binding to the coated solid phase is accomplished with the aid of the specifically bindable chemical group which is coupled to the sequence to be determined or to the polynucleotide probe sequence via a linker.
摘要翻译： 本发明描述了定量检测特定核苷酸序列的方法。 所述方法的基本特征在于，从混合物中分离出的单链核酸，特别是mRNA。 生物样品在溶液中杂交，多核苷酸序列与待测序列基本上互补; 然后将核酸固定在固相上，并测定结合的杂交体的量。 已经证明如果通过与所要测定的序列偶联的具体结合的化学基团或通过连接体与多核苷酸探针序列相结合来实现与被包被的固相的结合是特别有利的。
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4.发明授权
公开(公告)号：US5955271A
公开(公告)日：1999-09-21
申请号：US681015
申请日：1996-07-22
CPC分类号： C12Q1/6806
摘要： The invention involves methods and kits useful in determining specific mRNA molecules. The methods can be carried in a single reaction vessel, in that both reverse transcriptase and amplification can be carried out in the same, heat stable reaction vessel.
摘要翻译： 本发明涉及可用于确定特定mRNA分子的方法和试剂盒。 该方法可以在单个反应容器中进行，因为逆转录酶和扩增可以在相同的热稳定反应容器中进行。
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