公开(公告)号：US20120225478A1

公开(公告)日：2012-09-06

申请号：US13472781

申请日：2012-05-16

申请人： Michael Bardroff ,  Bernd Bohrmann ,  Manfred Brockhaus ,  Walter Huber ,  Titus Kretzschmar ,  Hansruedi Loetscher ,  Corinna Löhning ,  Christer Nordstedt ,  Christine Rothe

发明人： Michael Bardroff ,  Bernd Bohrmann ,  Manfred Brockhaus ,  Walter Huber ,  Titus Kretzschmar ,  Hansruedi Loetscher ,  Corinna Löhning ,  Christer Nordstedt ,  Christine Rothe

IPC分类号： C12N5/10 ,  C12N15/63 ,  C12N15/64 ,  C12N15/13

CPC分类号： C07K16/18 ,  A61K39/0007 ,  A61K2039/505 ,  C07K14/4711 ,  G01N33/6896

摘要： The present invention relates to antibody molecules capable of specifically recognizing two regions of the β-A4 peptide, wherein the first region comprises the amino acid sequence AEFRHDSGY as shown in SEQ ID NO: 1 or a fragment thereof and wherein the second region comprises the amino acid sequence VHHQKLVFFAEDVG as shown in SEQ ID NO: 2 or a fragment thereof. Furthermore, nucleic acid molecules encoding the inventive antibody molecules and vectors and hosts comprising said nucleic acid molecules are disclosed. In addition, the present invention provides for compositions, preferably pharmaceutical or diagnostic compositions, comprising the compounds of the invention as well as for specific uses of the antibody molecules, nucleic acid molecules, vectors or hosts of the invention.

摘要翻译： 本发明涉及能够特异性识别β-A4肽的两个区域的抗体分子，其中第一区域包含如SEQ ID NO：1所示的氨基酸序列AEFRHDSGY或其片段，其中第二区域包含 如SEQ ID NO：2所示的氨基酸序列VHHQKLVFFAEDVG或其片段。 此外，公开了编码本发明抗体分子的核酸分子和包含所述核酸分子的载体和宿主。 此外，本发明提供包含本发明化合物以及本发明的抗体分子，核酸分子，载体或宿主的特定用途的组合物，优选药物或诊断组合物。

公开(公告)号：US08216577B2

公开(公告)日：2012-07-10

申请号：US12462119

申请日：2009-07-28

申请人： Michael Bardroff ,  Bernd Bohrmann ,  Manfred Brockhaus ,  Walter Huber ,  Titus Kretzschmar ,  Hansruedi Loetscher ,  Corinna Löhning ,  Christer Nordstedt ,  Christine Rothe

发明人： Michael Bardroff ,  Bernd Bohrmann ,  Manfred Brockhaus ,  Walter Huber ,  Titus Kretzschmar ,  Hansruedi Loetscher ,  Corinna Löhning ,  Christer Nordstedt ,  Christine Rothe

IPC分类号： A61K39/395 ,  C07K16/18

CPC分类号： C07K16/18 ,  A61K39/0007 ,  A61K2039/505 ,  C07K14/4711 ,  G01N33/6896

摘要： The present invention relates to antibody molecules capable of specifically recognizing two regions of the β-A4 peptide, wherein the first region comprises the amino acid sequence AEFRHDSGY as shown in SEQ ID NO: 1 or a fragment thereof and wherein the second region comprises the amino acid sequence VHHQKLVFFAEDVG as shown in SEQ ID NO: 2 or a fragment thereof. Furthermore, nucleic acid molecules encoding the inventive antibody molecules and vectors and hosts comprising said nucleic acid molecules are disclosed. In addition, the present invention provides for compositions, preferably pharmaceutical or diagnostic compositions, comprising the compounds of the invention as well as for specific uses of the antibody molecules, nucleic acid molecules, vectors or hosts of the invention.

摘要翻译： 本发明涉及能够特异性识别β-A4肽的两个区域的抗体分子，其中第一区域包含如SEQ ID NO：1所示的氨基酸序列AEFRHDSGY或其片段，其中第二区域包含 如SEQ ID NO：2所示的氨基酸序列VHHQKLVFFAEDVG或其片段。 此外，公开了编码本发明抗体分子的核酸分子和包含所述核酸分子的载体和宿主。 此外，本发明提供包含本发明化合物以及本发明的抗体分子，核酸分子，载体或宿主的特定用途的组合物，优选药物或诊断组合物。

公开(公告)号：US20090252724A1

公开(公告)日：2009-10-08

申请号：US12086309

申请日：2006-12-11

申请人： Hansruedi Loetscher ,  Walter Huber ,  Diana Schuhbauer ,  Karl Weyer ,  Manfred Brockhaus ,  Bernd Bohrmann ,  Hans Koll ,  Andreas Schaubmar ,  Kurt Lang

发明人： Hansruedi Loetscher ,  Walter Huber ,  Diana Schuhbauer ,  Karl Weyer ,  Manfred Brockhaus ,  Bernd Bohrmann ,  Hans Koll ,  Andreas Schaubmar ,  Kurt Lang

IPC分类号： A61K39/395 ,  C07K16/00 ,  A61P25/28

CPC分类号： A61K39/3955 ,  A61K2039/505 ,  C07K16/18 ,  C07K2317/41 ,  C07K2317/56 ,  C07K2317/77

摘要： The present invention relates to a purified antibody molecule preparation being characterized in that at least one antigen binding site comprises a glycosylated asparagine (Asn) in the variable region of the heavy chain (VH). More specifically, a pharmaceutical and a diagnostic composition comprising said antibody molecule and antibody mixtures are provided which is/are capable of specifically recognizing the β-A4 peptide/Aβ4. The present invention relates in particular to a mixture of antibodies comprising one or two glycosylated antigen binding sites with a glycosylated asparagine (Asn) in the variable region of the heavy chain, i.e. mixtures of isoforms of antibodies which comprise a glycosylated Asn in the variable region of the heavy chain (VH). Also disclosed are compositions or antibody preparations comprising the specifically glycosylated antibody isoforms. Furthermore, the pharmaceutical and diagnostic uses for these antibodies are provided. The antibody isoforms may for example be used in the pharmaceutical intervention of amyloidogenesis or amyloid-plaque formation and/or in the diagnosis of the same.

摘要翻译： 本发明涉及纯化的抗体分子制剂，其特征在于至少一个抗原结合位点包含重链可变区（VH）中的糖基化天冬酰胺（Asn）。 更具体地，提供了包含所述抗体分子和抗体混合物的药物和诊断组合物，其能够特异性识别β-A4肽/Aβ4。 本发明特别涉及包含一个或两个糖基化抗原结合位点与重链可变区中的糖基化天冬酰胺（Asn）的抗体的混合物，即包含可变区中的糖基化Asn的抗体同种型的混合物 的重链（VH）。 还公开了包含特异性糖基化抗体同种型的组合物或抗体制剂。 此外，提供了这些抗体的药物和诊断用途。 抗体同种型可以例如用于淀粉样变形或淀粉样蛋白斑形成的药物干预和/或其诊断。

公开(公告)号：US20100306868A1

公开(公告)日：2010-12-02

申请号：US12740112

申请日：2008-10-23

申请人： Bernd Bohrmann ,  Antonio Iglesias ,  Hansruedi Loetscher

发明人： Bernd Bohrmann ,  Antonio Iglesias ,  Hansruedi Loetscher

IPC分类号： A01K67/027 ,  C12N5/07 ,  C12N15/89

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K2217/052 ,  A01K2217/15 ,  A01K2217/206 ,  A01K2227/105 ,  A01K2267/0312 ,  C07K14/4711 ,  C07K16/18

摘要： The present invention relates to a transgenic non-human animal whose genome comprises a) a first transgenic DNA sequence encoding a human APP Swedish or a human APP London protein, wherein said first transgenic DNA sequence is operably linked to a first promoter; b) a second transgenic DNA sequence encoding a human Presenilin 2 protein comprising a N141I substitution, wherein said second transgenic DNA sequence is operably linked to a second promoter; c) a third transgenic DNA sequence encoding a light chain of a antibody directed against the amyloid peptide, wherein said third transgenic DNA sequence is operably linked to a third promoter and; d) a forth transgenic DNA sequence encoding a heavy chain of said antibody, wherein said forth transgenic is operably linked to the third promoter or to a forth promoter, and methods for producing said animal.

摘要翻译： 本发明涉及一种转基因非人动物，其基因组包含a）编码人APP瑞典人或人APP伦敦蛋白的第一转基因DNA序列，其中所述第一转基因DNA序列与第一启动子可操作地连接; b）编码包含N141I取代的人类早老素2蛋白的第二转基因DNA序列，其中所述第二转基因DNA序列可操作地连接到第二启动子; c）编码针对淀粉样蛋白肽的抗体的轻链的第三转基因DNA序列，其中所述第三转基因DNA序列可操作地连接到第三启动子; d）编码所述抗体重链的第四个转基因DNA序列，其中所述第四个转基因可操作地连接到第三个启动子或第四个启动子，以及用于产生所述动物的方法。

公开(公告)号：US08329886B2

公开(公告)日：2012-12-11

申请号：US13472781

申请日：2012-05-16

申请人： Michael Bardroff ,  Bernd Bohrmann ,  Manfred Brockhaus ,  Walter Huber ,  Titus Kretzschmar ,  Hansruedi Loetscher ,  Corinna Löhning ,  Christer Nordstedt ,  Christine Rothe

发明人： Michael Bardroff ,  Bernd Bohrmann ,  Manfred Brockhaus ,  Walter Huber ,  Titus Kretzschmar ,  Hansruedi Loetscher ,  Corinna Löhning ,  Christer Nordstedt ,  Christine Rothe

IPC分类号： C07H21/00

CPC分类号： C07K16/18 ,  A61K39/0007 ,  A61K2039/505 ,  C07K14/4711 ,  G01N33/6896

摘要： The present invention relates to antibody molecules capable of specifically recognizing two regions of the β-A4 peptide, wherein the first region comprises the amino acid sequence AEFRHDSGY as shown in SEQ ID NO: 1 or a fragment thereof and wherein the second region comprises the amino acid sequence VHHQKLVFFAEDVG as shown in SEQ ID NO: 2 or a fragment thereof. Furthermore, nucleic acid molecules encoding the inventive antibody molecules and vectors and hosts comprising said nucleic acid molecules are disclosed. In addition, the present invention provides for compositions, preferably pharmaceutical or diagnostic compositions, comprising the compounds of the invention as well as for specific uses of the antibody molecules, nucleic acid molecules, vectors or hosts of the invention.

摘要翻译： 本发明涉及能够特异性识别β-A4肽的两个区域的抗体分子，其中第一区域包含如SEQ ID NO：1所示的氨基酸序列AEFRHDSGY或其片段，其中第二区域包含 如SEQ ID NO：2所示的氨基酸序列VHHQKLVFFAEDVG或其片段。 此外，公开了编码本发明抗体分子的核酸分子和包含所述核酸分子的载体和宿主。 此外，本发明提供包含本发明化合物以及本发明的抗体分子，核酸分子，载体或宿主的特定用途的组合物，优选药物或诊断组合物。

公开(公告)号：US20100172907A1

公开(公告)日：2010-07-08

申请号：US12462119

申请日：2009-07-28

申请人： Michael Bardroff ,  Bernd Bohrmann ,  Manfred Brockhaus ,  Walter Huber ,  Titus kretzschmar ,  Hansruedi Loetscher ,  Corinna Lohning ,  Christer Nordstedt ,  Christine Rothe

发明人： Michael Bardroff ,  Bernd Bohrmann ,  Manfred Brockhaus ,  Walter Huber ,  Titus kretzschmar ,  Hansruedi Loetscher ,  Corinna Lohning ,  Christer Nordstedt ,  Christine Rothe

IPC分类号： A61K39/395 ,  C07K16/00 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C12N1/15 ,  C12N1/19 ,  C12N5/10 ,  C12P21/00

CPC分类号： C07K16/18 ,  A61K39/0007 ,  A61K2039/505 ,  C07K14/4711 ,  G01N33/6896

摘要： The present invention relates to antibody molecules capable of specifically recognizing two regions of the β-A4 peptide, wherein the first region comprises the amino acid sequence AEFRHDSGY as shown in SEQ ID NO: 1 or a fragment thereof and wherein the second region comprises the amino acid sequence VHHQKLVFFAEDVG as shown in SEQ ID NO: 2 or a fragment thereof. Furthermore, nucleic acid molecules encoding the inventive antibody molecules and vectors and hosts comprising said nucleic acid molecules are disclosed. In addition, the present invention provides for compositions, preferably pharmaceutical or diagnostic compositions, comprising the compounds of the invention as well as for specific uses of the antibody molecules, nucleic acid molecules, vectors or hosts of the invention.

摘要翻译： 本发明涉及能够特异性识别β-A4肽的两个区域的抗体分子，其中第一区域包含如SEQ ID NO：1所示的氨基酸序列AEFRHDSGY或其片段，其中第二区域包含 如SEQ ID NO：2所示的氨基酸序列VHHQKLVFFAEDVG或其片段。 此外，公开了编码本发明抗体分子的核酸分子和包含所述核酸分子的载体和宿主。 此外，本发明提供包含本发明化合物以及本发明的抗体分子，核酸分子，载体或宿主的特定用途的组合物，优选药物或诊断组合物。

公开(公告)号：US08906370B2

公开(公告)日：2014-12-09

申请号：US12086309

申请日：2006-12-11

申请人： Hansruedi Loetscher ,  Walter Huber ,  Diana Schuhbauer ,  Karl Weyer ,  Manfred Brockhaus ,  Bernd Bohrmann ,  Hans Koll ,  Andreas Schaubmar ,  Kurt Lang

发明人： Hansruedi Loetscher ,  Walter Huber ,  Diana Schuhbauer ,  Karl Weyer ,  Manfred Brockhaus ,  Bernd Bohrmann ,  Hans Koll ,  Andreas Schaubmar ,  Kurt Lang

IPC分类号： A61K39/395 ,  C07K16/18 ,  A61P25/28 ,  A61K39/00

CPC分类号： A61K39/3955 ,  A61K2039/505 ,  C07K16/18 ,  C07K2317/41 ,  C07K2317/56 ,  C07K2317/77

摘要： The present invention relates to a purified antibody molecule preparation being characterized in that at least one antigen binding site comprises a glycosylated asparagine (Asn) in the variable region of the heavy chain (VH). More specifically, a pharmaceutical and a diagnostic composition comprising said antibody molecule and antibody mixtures are provided which is/are capable of specifically recognizing the β-A4 peptide/Aβ4. The present invention relates in particular to a mixture of antibodies comprising one or two glycosylated antigen binding sites with a glycosylated asparagine (Asn) in the variable region of the heavy chain, i.e. mixtures of isoforms of antibodies which comprise a glycosylated Asn in the variable region of the heavy chain (VH). Also disclosed are compositions or antibody preparations comprising the specifically glycosylated antibody isoforms. Furthermore, the pharmaceutical and diagnostic uses for these antibodies are provided. The antibody isoforms may for example be used in the pharmaceutical intervention of amyloidogenesis or amyloid-plaque formation and/or in the diagnosis of the same.

摘要翻译： 本发明涉及纯化的抗体分子制剂，其特征在于至少一个抗原结合位点包含重链可变区（VH）中的糖基化天冬酰胺（Asn）。 更具体地，提供了包含所述抗体分子和抗体混合物的药物和诊断组合物，其能够特异性识别Aβ-4肽/ A＆bgr; 4。 本发明特别涉及包含一个或两个糖基化抗原结合位点与重链可变区中的糖基化天冬酰胺（Asn）的抗体的混合物，即包含可变区中的糖基化Asn的抗体同种型的混合物 的重链（VH）。 还公开了包含特异性糖基化抗体同种型的组合物或抗体制剂。 此外，提供了这些抗体的药物和诊断用途。 抗体同种型可以例如用于淀粉样变形或淀粉样蛋白斑形成的药物干预和/或其诊断。

公开(公告)号：US07794719B2

公开(公告)日：2010-09-14

申请号：US10505313

申请日：2003-02-20

申请人： Michael Bardroff ,  Bernd Bohrmann ,  Manfred Brockhaus ,  Walter Huber ,  Titus Kretzschmar ,  Hansruedi Loetscher ,  Corinna Löhning ,  Christer Nordstedt ,  Christine Rothe

发明人： Michael Bardroff ,  Bernd Bohrmann ,  Manfred Brockhaus ,  Walter Huber ,  Titus Kretzschmar ,  Hansruedi Loetscher ,  Corinna Löhning ,  Christer Nordstedt ,  Christine Rothe

IPC分类号： A61K39/395 ,  C07K16/18

CPC分类号： C07K16/18 ,  A61K39/0007 ,  A61K2039/505 ,  C07K14/4711 ,  G01N33/6896

摘要： The present invention relates to antibody molecules capable of specifically recognizing two regions of the R-A4 peptide, wherein the first region comprises the amino acid sequence AEFRHDSGY as shown in SEQ ID NO: 1 or a fragment thereof and wherein the second region comprises the amino acid sequence VHHQKLVFFAEDVG as shown in SEQ ID NO: 2 or a fragment thereof. Furthermore, nucleic acid molecules encoding the inventive antibody molecules and vectors and hosts comprising said nucleic acid molecules are disclosed. In addition, the present invention provides for compositions, preferably pharmaceutical or diagnostic compositions, comprising the compounds of the invention as well as for specific uses of the antibody molecules, nucleic acid molecules, vectors or hosts of the invention.

摘要翻译： 本发明涉及能够特异性识别R-A4肽的两个区域的抗体分子，其中第一区域包含如SEQ ID NO：1所示的氨基酸序列AEFRHDSGY或其片段，其中第二区域包含氨基酸 酸序列VHHQKLVFFAEDVG，如SEQ ID NO：2所示或其片段。 此外，公开了编码本发明抗体分子的核酸分子和包含所述核酸分子的载体和宿主。 此外，本发明提供包含本发明化合物以及本发明的抗体分子，核酸分子，载体或宿主的特定用途的组合物，优选药物或诊断组合物。

公开(公告)号：US20050169925A1

公开(公告)日：2005-08-04

申请号：US10505313

申请日：2003-02-20

申请人： Michael Bardroff ,  Bernd Bohrmann ,  Manfred Brockhaus ,  Walter Huber ,  Titus Kretzschmar ,  Hansruedi Loetscher ,  Corinna Lohning ,  Christer Nordstedt ,  Christine Rothe

发明人： Michael Bardroff ,  Bernd Bohrmann ,  Manfred Brockhaus ,  Walter Huber ,  Titus Kretzschmar ,  Hansruedi Loetscher ,  Corinna Lohning ,  Christer Nordstedt ,  Christine Rothe

IPC分类号： C12N15/09 ,  A61K39/00 ,  A61K39/395 ,  A61P25/00 ,  A61P25/28 ,  C07K14/47 ,  C07K16/18 ,  C07K16/46 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P21/08 ,  G01N33/68 ,  C07K16/40

CPC分类号： C07K16/18 ,  A61K39/0007 ,  A61K2039/505 ,  C07K14/4711 ,  G01N33/6896

摘要： The present invention relates to antibody molecules capable of specifically recognizing two regions of the R-A4 peptide, wherein the first region comprises the amino acid sequence ABFRHDSGY as shown in SEQ ID NO: 1 or a fragment thereof and wherein the second region comprises the amino acid sequence VHHQKLVFFAEDVG as shown in SEQ ID NO: 2 or a fragment thereof. Furthermore, nucleic acid molecules encoding the inventive antibody molecules and vectors and hosts comprising said nucleic acid molecules are disclosed. In addition, the present invention provides for compositions, preferably pharmaceutical or diagnostic compositions, comprising the compounds of the invention as well as for specific uses of the antibody molecules, nucleic acid molecules, vectors or hosts of the invention.

摘要翻译： 本发明涉及能够特异性识别R-A4肽的两个区域的抗体分子，其中第一区域包含如SEQ ID NO：1所示的氨基酸序列ABFRHDSGY或其片段，其中第二区域包含氨基酸 酸序列VHHQKLVFFAEDVG，如SEQ ID NO：2所示或其片段。 此外，公开了编码本发明抗体分子的核酸分子和包含所述核酸分子的载体和宿主。 此外，本发明提供包含本发明化合物以及本发明的抗体分子，核酸分子，载体或宿主的特定用途的组合物，优选药物或诊断组合物。