公开(公告)号：US08669068B2

公开(公告)日：2014-03-11

申请号：US13806101

申请日：2011-12-12

申请人： Haohui Kong ,  Yifei Huang ,  Baofeng Jin

发明人： Haohui Kong ,  Yifei Huang ,  Baofeng Jin

IPC分类号： C12Q1/34 ,  C12P7/06 ,  C12M1/36 ,  C12M1/38 ,  C12M3/00 ,  C12M1/34 ,  C12M1/00

CPC分类号： G01N21/78 ,  C12Q1/34 ,  G01N1/4005 ,  G01N1/4044 ,  G01N2333/415 ,  G01N2333/42 ,  G01N2333/924 ,  Y02E50/17

摘要： A method for determining pectin content in a plant sample includes: 1) adding an acidic alcohol solution to the plant sample, heating in a water bath and filtration; 2) soaking residue from step 1 with an acidic solution, heating in a water bath, a second filtration, bringing to volume after cooling and obtaining filtrate; 3) adding an acetic acid/sodium acetate buffer solution to residue of step 2, adding a pectinase solution, heating under vibration in a water bath, and a third filtration to obtain a filtrate; 4) adding an acetic acid/sodium acetate buffer solution and a pectinase solution to filtrate from step 2, heating the mixture under vibration in a water bath to obtain an enzymatic hydrolysate, adding filtrate obtained in step 3 to the enzymatic hydrolysate, bringing to volume, and obtaining a test solution; 5) drawing the test solution into a continuous flow analyzer to perform analysis.

摘要翻译： 确定植物样品中果胶含量的方法包括：1）向植物样品中加入酸性醇溶液，在水浴中加热过滤; 2）用酸性溶液浸渍步骤1的残余物，在水浴中加热，第二次过滤，冷却后体积，得到滤液; 3）向步骤2的残留物中加入乙酸/乙酸钠缓冲溶液，加入果胶酶溶液，在水浴中振动加热，并进行第三次过滤，得到滤液; 4）向步骤2的滤液中加入乙酸/乙酸钠缓冲液和果胶酶溶液，在水浴中振动加热混合物，得到酶水解产物，将步骤3中得到的滤液加入到酶水解产物中， ，并获得测试溶液; 5）将测试溶液拉入连续流量分析仪进行分析。

公开(公告)号：US20130143249A1

公开(公告)日：2013-06-06

申请号：US13806101

申请日：2011-12-12

申请人： Haohui Kong ,  Yifei Huang ,  Baofeng Jin

发明人： Haohui Kong ,  Yifei Huang ,  Baofeng Jin

IPC分类号： G01N21/78

CPC分类号： G01N21/78 ,  C12Q1/34 ,  G01N1/4005 ,  G01N1/4044 ,  G01N2333/415 ,  G01N2333/42 ,  G01N2333/924 ,  Y02E50/17

摘要： Disclosed is a method for determining pectin content in a plant sample, comprising the following steps: 1) adding an acidic alcohol solution to the plant sample, then heating the resulting mixture to reflux in a water bath, followed by a first filtration; 2) soaking the filtered residue obtained from the first filtration with an acidic solution, then heating the resulting mixture to reflux in a water bath, followed by a second filtration, then bringing to volume after cooling, obtaining filtrate for later use; 3) adding an acetic acid/sodium acetate buffer solution to treat the filtered residue obtained from the second filtration, then adding a pectinase solution and heating the resulting mixture under vibration in a water bath, followed by a third filtration to obtain a filtrate for later use; 4) sequentially adding an acetic acid/sodium acetate buffer solution and a pectinase solution to the filtrate obtained in step 2) and heating the resulting mixture under vibration in a water bath to obtain an enzymatic hydrolysate, then adding the filtrate obtained in step 3) to the enzymatic hydrolysate followed by bringing to volume, obtaining a test solution; and 5) drawing the test solution into a continuous flow analyzer to perform analysis. The method disclosed has the advantage of providing accurate analysis results.

摘要翻译： 公开了一种测定植物样品中果胶含量的方法，包括以下步骤：1）向植物样品中加入酸性醇溶液，然后将所得混合物在水浴中加热回流，然后进行第一次过滤; 2）用酸性溶液浸渍从第一次过滤获得的过滤残留物，然后在水浴中将所得混合物加热回流，然后进行第二次过滤，然后冷却后使其体积，得到滤液以备后用; 3）加入乙酸/乙酸钠缓冲溶液处理从第二次过滤获得的过滤残渣，然后加入果胶酶溶液，并在水浴中振动加热所得混合物，然后进行第三次过滤，得到滤液 使用; 4）依次向步骤2）中得到的滤液中加入乙酸/乙酸钠缓冲液和果胶酶溶液，并在水浴中振动加热所得混合物，得到酶水解产物，然后加入步骤3）中得到的滤液， 到酶水解产物，然后调至体积，得到测试溶液; 和5）将测试溶液拉入连续流量分析仪进行分析。 所公开的方法具有提供精确分析结果的优点。