公开(公告)号：US20050281833A1

公开(公告)日：2005-12-22

申请号：US11180245

申请日：2005-07-13

申请人： Rajendra Singh ,  Harshvardhan Mehta

发明人： Rajendra Singh ,  Harshvardhan Mehta

IPC分类号： A01N43/16 ,  G01N33/564 ,  A61K39/00 ,  C07K14/705 ,  C07K16/46

CPC分类号： C08B37/0021 ,  A01N43/16 ,  C08H1/00 ,  G01N33/564 ,  G01N2333/988

摘要： Compounds that are modified polysaccharides having pendant aldehyde functionalities are disclosed. Each of the aldehyde functionalities is attached through a linker to a position corresponding to a hydrogen atom of a different hydroxyl group of unmodified polysaccharide. Also disclosed is a method fore introducing an amine-reactive functionality into a dextran. The method comprises (a) reacting the dextran with an alkylating agent having a functionality that reacts with an hydroxyl group of the dextran thereby forming an alkylated dextran wherein the alkylating agent has an olefin group and (b) treating the alkylated dextran to convert the olefin group to an amine-reactive functionality. A polysaccharide can be conjugated to a biomolecule by carrying out the above method and reacting the amine-reactive functionality with an amine functionality on the biomolecule to produce polysaccharide conjugated to the biomolecule.

摘要翻译： 公开了具有侧醛官能团的改性多糖的化合物。 每个醛官能团通过接头连接到对应于未修饰多糖的不同羟基的氢原子的位置。 还公开了将胺反应性功能引入葡聚糖的方法。 该方法包括（a）使葡聚糖与具有与葡聚糖羟基反应的官能团的烷基化剂反应，从而形成烷基化葡聚糖，其中烷基化剂具有烯烃基团，和（b）处理烷基化葡聚糖以转化烯烃 与胺反应性官能团。 通过进行上述方法并使胺反应性官能团与胺官能团在生物分子上反应以产生与生物分子缀合的多糖，可以将多糖与生物分子缀合。

公开(公告)号：US20090246225A1

公开(公告)日：2009-10-01

申请号：US12330299

申请日：2008-12-08

申请人： George Robert Trager ,  George Kemble ,  Richard M. Schwartz ,  Harshvardhan Mehta ,  Vu Truong-Le ,  Zhongying Chen ,  Alfred A. Pan ,  Eric Tsao ,  Chiaoyin Kathy Wang ,  Luisa Yee ,  Palani Balu

发明人： George Robert Trager ,  George Kemble ,  Richard M. Schwartz ,  Harshvardhan Mehta ,  Vu Truong-Le ,  Zhongying Chen ,  Alfred A. Pan ,  Eric Tsao ,  Chiaoyin Kathy Wang ,  Luisa Yee ,  Palani Balu

IPC分类号： A61K39/145 ,  A61P31/16

CPC分类号： A61K39/145 ,  A61K35/76 ,  A61K39/12 ,  A61K47/02 ,  A61K47/183 ,  A61K47/26 ,  A61K47/42 ,  A61K2039/525 ,  A61K2039/5254 ,  B01D61/145 ,  B01D61/147 ,  C12N7/00 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16234 ,  C12N2760/16251

摘要： Methods and compositions for the optimization of production of influenza viruses suitable as influenza vaccines are provided.

摘要翻译： 提供了适合作为流感疫苗优化流感病毒生产的方法和组合物。

公开(公告)号：US20070161085A1

公开(公告)日：2007-07-12

申请号：US11668587

申请日：2007-01-30

申请人： George Trager ,  George Kemble ,  Richard Schwartz ,  Harshvardhan Mehta ,  Vu Truong-Le ,  Zhongying Chen ,  Alfred Pan ,  Eric Tsao ,  Chiaoyin Wang ,  Luisa Yee ,  Palani Balu

发明人： George Trager ,  George Kemble ,  Richard Schwartz ,  Harshvardhan Mehta ,  Vu Truong-Le ,  Zhongying Chen ,  Alfred Pan ,  Eric Tsao ,  Chiaoyin Wang ,  Luisa Yee ,  Palani Balu

IPC分类号： C12Q1/68 ,  C12P21/06

CPC分类号： A61K39/145 ,  A61K35/76 ,  A61K39/12 ,  A61K47/02 ,  A61K47/183 ,  A61K47/26 ,  A61K47/42 ,  A61K2039/525 ,  A61K2039/5254 ,  B01D61/145 ,  B01D61/147 ,  C12N7/00 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16234 ,  C12N2760/16251

摘要： Methods and compositions for the optimization of production of influenza viruses suitable as influenza vaccines are provided.

摘要翻译： 提供了适合作为流感疫苗优化流感病毒生产的方法和组合物。

公开(公告)号：US06949524B2

公开(公告)日：2005-09-27

申请号：US10188578

申请日：2002-07-02

申请人： Rajendra Singh ,  Harshvardhan Mehta

发明人： Rajendra Singh ,  Harshvardhan Mehta

IPC分类号： A01N43/16 ,  G01N33/564 ,  A61K31/715 ,  C08B37/00

CPC分类号： C08B37/0021 ,  A01N43/16 ,  C08H1/00 ,  G01N33/564 ,  G01N2333/988

摘要： Compounds that are modified polysaccharides having pendant aldehyde functionalities are disclosed. Each of the aldehyde functionalities is attached through a linker to a position corresponding to a hydrogen atom of a different hydroxyl group of unmodified polysaccharide. Also disclosed is a method for introducing an amine-reactive functionality into a dextran. The method comprises (a) reacting the dextran with an alkylating agent having a functionality that reacts with an hydroxyl group of the dextran thereby forming an alkylated dextran wherein the alkylating agent has an olefin group and (b) treating the alkylated dextran to convert the olefin group to an amine-reactive functionality. A polysaccharide can be conjugated to a biomolecule by carrying out the above method and reacting the amine-reactive functionality with an amine functionality on the biomolecule to produce polysaccharide conjugated to the biomolecule.

摘要翻译： 公开了具有侧醛官能团的改性多糖的化合物。 每个醛官能团通过接头连接到对应于未修饰多糖的不同羟基的氢原子的位置。 还公开了将胺反应性功能引入葡聚糖的方法。 该方法包括（a）使葡聚糖与具有与葡聚糖羟基反应的官能团的烷基化剂反应，从而形成烷基化葡聚糖，其中烷基化剂具有烯烃基团，和（b）处理烷基化葡聚糖以转化烯烃 与胺反应性官能团。 通过进行上述方法并使胺反应性官能团与胺官能团在生物分子上反应以产生与生物分子缀合的多糖，可以将多糖与生物分子缀合。

公开(公告)号：US06489309B1

公开(公告)日：2002-12-03

申请号：US09314055

申请日：1999-05-18

申请人： Rajendra Singh ,  Harshvardhan Mehta

发明人： Rajendra Singh ,  Harshvardhan Mehta

IPC分类号： A01N4304

CPC分类号： C08B37/0021 ,  A01N43/16 ,  C08H1/00 ,  G01N33/564 ,  G01N2333/988

摘要： Compounds that are modified polysaccharides having pendant aldehyde functionalities are disclosed. Each of the aldehyde functionalities is attached through a linker to a position corresponding to a hydrogen atom of a different hydroxyl group of unmodified polysaccharide. Also disclosed is a method for introducing an amine-reactive functionality into a dextran. The method comprises (a) reacting the dextran with an alkylating agent having a functionality that reacts with an hydroxyl group of the dextran thereby forming an alkylated dextran wherein the alkylating agent has an olefin group and (b) treating the alkylated dextran to convert the olefin group to an amine-reactive functionality. A polysaccharide can be conjugated to a biomolecule by carrying out the above method and reacting the amine-reactive functionality with an amine functionality on the biomolecule to produce polysaccharide conjugated to the biomolecule.

摘要翻译： 公开了具有侧醛官能团的改性多糖的化合物。 每个醛官能团通过接头连接到对应于未修饰多糖的不同羟基的氢原子的位置。 还公开了将胺反应性功能引入葡聚糖的方法。 该方法包括（a）使葡聚糖与具有与葡聚糖羟基反应的官能团的烷基化剂反应，从而形成烷基化葡聚糖，其中烷基化剂具有烯烃基团，和（b）处理烷基化葡聚糖以转化烯烃 与胺反应性官能团。 通过进行上述方法并使胺反应性官能团与胺官能团在生物分子上反应以产生与生物分子缀合的多糖，可以将多糖与生物分子缀合。

公开(公告)号：US5929049A

公开(公告)日：1999-07-27

申请号：US907842

申请日：1997-08-08

申请人： Rajendra Singh ,  Harshvardhan Mehta

发明人： Rajendra Singh ,  Harshvardhan Mehta

IPC分类号： A01N43/16 ,  G01N33/564 ,  A01N43/04

CPC分类号： C08B37/0021 ,  A01N43/16 ,  C08H1/00 ,  G01N33/564 ,  G01N2333/988

摘要： Compounds that are modified polysaccharides having pendant aldehyde functionalities are disclosed. Each of the aldehyde functionalities is attached through a linker to a position corresponding to a hydrogen atom of a different hydroxyl group of unmodified polysaccharide. Also disclosed is a method for introducing an amine-reactive functionality into a dextran. The method comprises (a) reacting the dextran with an alkylating agent having a functionality that reacts with an hydroxyl group of the dextran thereby forming an alkylated dextran wherein the alkylating agent has an olefin group and (b) treating the alkylated dextran to convert the olefin group to an amine-reactive functionality. A polysaccharide can be conjugated to a biomolecule by carrying out the above method and reacting the amine-reactive functionality with an amine functionality on the biomolecule to produce polysaccharide conjugated to the biomolecule.