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公开(公告)号：US09334515B2
公开(公告)日：2016-05-10
申请号：US13813154
申请日：2011-07-14
申请人： Joel Edward Graham , Melinda E. Clark , Frank Thomson Robb , Douglas S. Clark , Harvey W. Blanch
发明人： Joel Edward Graham , Melinda E. Clark , Frank Thomson Robb , Douglas S. Clark , Harvey W. Blanch
IPC分类号： C12N9/26 , C12P7/00 , C12N15/00 , C12N1/20 , C07H21/04 , C12P19/14 , C12P19/02 , C13K1/02 , C12N9/42 , C12N9/24
CPC分类号： C12P19/14 , C12N9/2402 , C12N9/2437 , C12P7/00 , C12P19/02 , C13K1/02
摘要： Hydrolysis and degradation of cellulose-containing biomass by use of a polypeptide having cellulase activity is provided. Also provided are polypeptides having cellulase activity, such as archaeal cellulases, polynucleotides encoding the polypeptides, and compositions containing the polypeptides, and methods of use thereof.
摘要翻译： 提供了通过使用具有纤维素酶活性的多肽来水解和降解含纤维素的生物质。 还提供了具有纤维素酶活性的多肽，例如古细菌纤维素酶，编码多肽的多核苷酸，以及含有该多肽的组合物及其使用方法。
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2.发明申请公开(公告)号：US20120315695A1
公开(公告)日：2012-12-13
申请号：US13434714
申请日：2012-03-29
CPC分类号： C13K1/02 , A23V2002/00 , A23V2250/60
摘要： The present invention provides for a composition comprising a solution comprising (a) an ionic liquid (IL) or ionic liquid-aqueous (ILA) phase and (b) an organic phase, wherein the solution comprises a sugar and a boronic acid. The present invention also provides for a method of removing a sugar from a solution, comprising: (a) providing a solution comprising (i) an IL or ILA phase and (ii) an organic phase, wherein the solution comprises an IL, a sugar and a boronic acid; (b) contacting the sugar with the boronic acid to form a sugar-boronic acid complex, (c) separating the organic phase and the aqueous phase, wherein the organic phase contains the sugar-boronic acid complex, and optionally (d) separating the sugar from the organic phase.
摘要翻译： 本发明提供一种包含溶液的组合物，所述溶液包含（a）离子液体（IL）或离子液体 - 水（ILA）相和（b）有机相，其中所述溶液包含糖和硼酸。 本发明还提供从溶液中除去糖的方法，其包括：（a）提供包含（i）IL或ILA相和（ii）有机相的溶液，其中所述溶液包含IL，糖 和硼酸; （b）使糖与硼酸接触以形成糖 - 硼酸络合物，（c）分离有机相和水相，其中有机相含有糖 - 硼酸络合物，和任选地（d）将 有机相糖。
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3.发明申请THERMOSTABLE CELLULASES, AND MUTANTS THEREOF, CAPABLE OF HYDROLYZING CELLULOSE IN IONIC LIQUID 有权可热膨胀的细胞，以及其可溶性离子液体中的水解纤维素公开(公告)号：US20120129227A1
公开(公告)日：2012-05-24
申请号：US13265786
申请日：2010-04-23
申请人： Rajat Sapra , Supratim Datta , Zhiwei Chen , Bradley M. Holmes , Blake A. Simmons , Harvey W. Blanch
发明人： Rajat Sapra , Supratim Datta , Zhiwei Chen , Bradley M. Holmes , Blake A. Simmons , Harvey W. Blanch
CPC分类号： C12P19/14 , C12N9/2437 , C12Y302/01004 , Y02P20/52
摘要： The present invention provides for a composition comprising an ionic liquid and a thermostable cellulose, and a method of hydrolyzing a cellulose, comprising: (a) providing a composition comprising a solution comprising an ionic liquid and a cellulose, and (b) introducing a thermostable cellulase to the solution, such that the cellulose is hydrolyzed by the cellulase. The present invention also provides for a Thermatoga maritima thermostable cellulase mutant with increased cellulase activity.
摘要翻译： 本发明提供一种包含离子液体和耐热纤维素的组合物和水解纤维素的方法，其包括：（a）提供包含离子液体和纤维素的溶液的组合物，和（b）引入热稳定剂 纤维素酶溶解，使得纤维素被纤维素酶水解。 本发明还提供了具有增加的纤维素酶活性的Thermatoga maritima热稳定纤维素酶突变体。
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公开(公告)号：US5073575A
公开(公告)日：1991-12-17
申请号：US237392
申请日：1988-08-26
申请人： Harvey W. Blanch , John Yin
发明人： Harvey W. Blanch , John Yin
CPC分类号： B01J45/00 , C02F1/285 , C08F8/34 , C08G18/838 , C08G63/688 , C08G64/081
摘要： The present invention relates to an adsorbent polymer having improved selectivity for cadmium ion, comprising a water-insoluble polymeric material having available surface pendant chelating groups having a multiplicity of thiol (--SH) groups, wherein pairs of thiol groups are connected by a chain R having between about 10 and 16 atoms of the structure: ##STR1## which thiol terminated chains are capable of chelating cadmium ion in rings of between about 11 and 17 atoms, andA is selected from alkylene having from 1 to 10 carbon atoms, cycloalkylene, aryl or arylalkylene. Preferably the adsorbent polymer having improved selectivity for cadmium of claim 1, wherein the pendant chelating groups having a thiol structure: ##STR2##BACKGROUND OF INVENTION1. Origin of the InventionPortions of the research leading to this invention were funded by the U.S. Environmental Protection Agency (Cooperative Agreement No. CR 811984-01-0) and the University of California (Berkeley) Chancellor's Patent Fund (No. 401220-09520).
摘要翻译： 本发明涉及对镉离子具有改善的选择性的吸附剂聚合物，其包含具有多个硫醇（-SH）基团的可用表面侧链螯合基团的水不溶性聚合物材料，其中硫醇基对通过链R连接 具有约10至16个结构原子：其中硫醇封端链能够在约11至17个原子的环中螯合镉离子，并且A选自具有1至10个碳原子的亚烷基，亚环烷基， 芳基或芳基亚烷基。 优选地，具有改进的权利要求1的镉选择性的吸附剂聚合物，其中具有硫醇结构的侧螯合基团：本发明还涉及从含有镉离子的水溶液中除去镉离子的方法，该方法包括 （a）使含有镉离子的水溶液与有效量的具有多个硫醇（-SH）基团的具有可利用的表面侧挂螯合基团的水不溶性固体材料接触，其中硫醇基对通过具有 在结构（I）的约10和16个原子之间的时间对于硫醇基团对结合镉离子有效; 和（b）分离具有减少的镉离子含量的固体材料和水溶液。
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5.发明授权Method of producing products by enzyme-catalyzed reactions in supercritical fluids 失效超临界流体酶催化反应生产产物的方法
公开(公告)号：US4925790A
公开(公告)日：1990-05-15
申请号：US771366
申请日：1985-08-30
CPC分类号： B01J3/008 , C12P1/00 , Y02P20/544 , Y10S435/801 , Y10S435/813
摘要： A method is provided for producing a product by enzyme-catalyzed reaction using supercritical fluid as a solvent. Improved reaction times, conversion efficiencies and/or other efficiencies (such as improved ease of product recovery) are obtained.
摘要翻译： 提供了使用超临界流体作为溶剂通过酶催化反应制备产物的方法。 获得了改进的反应时间，转化效率和/或其他效率（例如提高产物回收的容易程度）。
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公开(公告)号：US09410135B2
公开(公告)日：2016-08-09
申请号：US13822623
申请日：2011-08-11
CPC分类号： C12N9/2437 , C12N9/244 , C12P7/10 , C12P19/14 , C12P2201/00 , C12Y302/01004 , C12Y302/01006 , Y02E50/16
摘要： The present disclosure relates to mutant thermostable glycosyl hydrolases family 7 enzymes, including mutant Trichoderma reesei endoglucanase I. In particular, the present disclosure relates to mutant thermostable enzymes, compositions containing the enzymes, and methods of use thereof.
摘要翻译： 本公开涉及突变型热稳定糖基水解酶家族7酶，包括突变型里氏木霉内切葡聚糖酶I.特别地，本公开涉及突变热稳定酶，含有酶的组合物及其使用方法。
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公开(公告)号：US20150211037A1
公开(公告)日：2015-07-30
申请号：US13813154
申请日：2011-07-14
申请人： Joel Edward Graham , Melinda E. Clark , Frank Thomson Robb , Douglas S. Clark , Harvey W. Blanch
发明人： Joel Edward Graham , Melinda E. Clark , Frank Thomson Robb , Douglas S. Clark , Harvey W. Blanch
CPC分类号： C12P19/14 , C12N9/2402 , C12N9/2437 , C12P7/00 , C12P19/02 , C13K1/02
摘要： Hydrolysis and degradation of cellulose-containing biomass by use of a polypeptide having cellulase activity is provided. Also provided are polypeptides having cellulase activity, such as archaeal cellulases, polynucleotides encoding the polypeptides, and compositions containing the polypeptides, and methods of use thereof.
摘要翻译： 提供了通过使用具有纤维素酶活性的多肽来水解和降解含纤维素的生物质。 还提供了具有纤维素酶活性的多肽，例如古细菌纤维素酶，编码多肽的多核苷酸，以及含有该多肽的组合物及其使用方法。
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8.发明授权Thermostable cellulases, and mutants thereof, capable of hydrolyzing cellulose in ionic liquid 有权能够在离子液体中水解纤维素的热稳定纤维素酶及其突变体公开(公告)号：US09322042B2
公开(公告)日：2016-04-26
申请号：US13265786
申请日：2010-04-23
申请人： Rajat Sapra , Supratim Datta , Zhiwei Chen , Bradley M. Holmes , Blake A. Simmons , Harvey W. Blanch
发明人： Rajat Sapra , Supratim Datta , Zhiwei Chen , Bradley M. Holmes , Blake A. Simmons , Harvey W. Blanch
CPC分类号： C12P19/14 , C12N9/2437 , C12Y302/01004 , Y02P20/52
摘要： The present invention provides for a composition comprising an ionic liquid and a thermostable cellulose, and a method of hydrolyzing a cellulose, comprising: (a) providing a composition comprising a solution comprising an ionic liquid and a cellulose, and (b) introducing a thermostable cellulase to the solution, such that the cellulose is hydrolyzed by the cellulase. The present invention also provides for a Thermatoga maritima thermostable cellulase mutant with increased cellulase activity.
摘要翻译： 本发明提供一种包含离子液体和耐热纤维素的组合物和水解纤维素的方法，其包括：（a）提供包含离子液体和纤维素的溶液的组合物，和（b）引入热稳定剂 纤维素酶溶解，使得纤维素被纤维素酶水解。 本发明还提供了具有增加的纤维素酶活性的Thermatoga maritima热稳定纤维素酶突变体。
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9.发明授权公开(公告)号：US09157130B2
公开(公告)日：2015-10-13
申请号：US13434714
申请日：2012-03-29
CPC分类号： C13K1/02 , A23V2002/00 , A23V2250/60
摘要： The present invention provides for a composition comprising a solution comprising (a) an ionic liquid (IL) or ionic liquid-aqueous (ILA) phase and (b) an organic phase, wherein the solution comprises a sugar and a boronic acid. The present invention also provides for a method of removing a sugar from a solution, comprising: (a) providing a solution comprising (i) an IL or ILA phase and (ii) an organic phase, wherein the solution comprises an IL, a sugar and a boronic acid; (b) contacting the sugar with the boronic acid to form a sugar-boronic acid complex, (c) separating the organic phase and the aqueous phase, wherein the organic phase contains the sugar-boronic acid complex, and optionally (d) separating the sugar from the organic phase.
摘要翻译： 本发明提供一种包含溶液的组合物，所述溶液包含（a）离子液体（IL）或离子液体 - 水（ILA）相和（b）有机相，其中所述溶液包含糖和硼酸。 本发明还提供从溶液中除去糖的方法，其包括：（a）提供包含（i）IL或ILA相和（ii）有机相的溶液，其中所述溶液包含IL，糖 和硼酸; （b）使糖与硼酸接触以形成糖 - 硼酸络合物，（c）分离有机相和水相，其中有机相含有糖 - 硼酸络合物，和任选地（d）将 有机相糖。
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10.发明申请Novel method of acid hydrolysis of biomass and the recovery of sugars thereof by solvent extraction 审中-公开通过溶剂萃取，生物质酸水解的新方法及其糖的回收公开(公告)号：US20120301948A1
公开(公告)日：2012-11-29
申请号：US13436704
申请日：2012-03-30
CPC分类号： C07H3/02 , C07H1/08 , C07H3/04 , C08B15/00 , C08B37/0057 , C08H8/00 , C12N1/22 , C13K1/02 , C13K13/002
摘要： The present invention provides for a method of hydrolyzing a cellulose, hemicellulose, or ligno-cellulose comprising: (a) contacting (i) an ionic liquid (IL) or ionic liquid-aqueous (ILA) phase comprising cellulose, hemicellulose, or ligno-cellulose, or a mixture thereof, and (ii) an acid, such that the cellulose, hemicellulose, or ligno-cellulose is hydrolyzed into sugar, and (b) optionally adding water to the IL or ILA phase wherein the proportion of water in the IL or ILA phase does not exceed about 60% by weight. The present invention also provides for a method of recovering a sugar comprising contacting an IL or ILA phase and an organic phase comprising an organic acid.
摘要翻译： 本发明提供水解纤维素，半纤维素或木质纤维素的方法，其包括：（a）使包含纤维素，半纤维素或木质素的离子液体（IL）或离子液体 - 纤维素或其混合物，和（ii）酸，使得纤维素，半纤维素或木质纤维素被水解成糖，和（b）任选地将水添加到IL或ILA相中，其中水的比例 IL或ILA相不超过约60重量％。 本发明还提供了一种回收糖的方法，包括使IL或ILA相和包含有机酸的有机相接触。
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