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公开(公告)号:US5176995A
公开(公告)日:1993-01-05
申请号:US394145
申请日:1989-08-15
CPC分类号: B01L7/52 , C07K14/805 , C12N15/10 , C12Q1/6823 , C12Q1/6853 , C12Q1/686 , C12Q1/6865 , C12Q1/6876 , C12Q1/6881 , C12Q1/6883 , C12Q1/70 , C12Q1/703 , C12Q1/706 , B01J2219/00495 , B01J2219/0059 , B01J2219/00722 , C40B40/06 , C40B60/14 , Y10S435/81 , Y10S436/811
摘要: The presence or absence of a nucleic acid sequence associated with one or more related viruses in a sample containing one or more nucleic acids and suspected of containing such sequence can be detected by amplifying the sequence using primers to form extension products as templates and detecting the amplified product if it is present. This may be accomplished by adding a labeled hybridization probe to the amplified product either free in solution or after immobilization on a solid support. Preferably the virus constitutes AIDs viruses and hepadnaviruses.
摘要翻译: 可以通过使用引物扩增序列来检测含有一种或多种核酸并且怀疑含有该序列的样品中与一种或多种相关病毒相关的核酸序列,以形成扩增产物作为模板并检测扩增的 产品如果存在的话。 这可以通过将标记的杂交探针加入到在溶液中游离或固定在固体支持物上的扩增产物来实现。 优选地,病毒构成AIDs病毒和肝炎病毒。