公开(公告)号：US20140323324A1

公开(公告)日：2014-10-30

申请号：US13994435

申请日：2011-12-16

申请人： Hisashi Narimatsu ,  Jun Hirabayashi ,  Atsushi Kuno ,  Hideki Matsuzaki ,  Yuzuru Ikehara ,  Hiromi Ito ,  Yasuhiro Hashimoto ,  Keiro Shirotani ,  Satoshi Futakawa ,  Hajime Arai ,  Masakazu Miyajima ,  Kazuo Fujihara

发明人： Hisashi Narimatsu ,  Jun Hirabayashi ,  Atsushi Kuno ,  Hideki Matsuzaki ,  Yuzuru Ikehara ,  Hiromi Ito ,  Yasuhiro Hashimoto ,  Keiro Shirotani ,  Satoshi Futakawa ,  Hajime Arai ,  Masakazu Miyajima ,  Kazuo Fujihara

IPC分类号： G01N33/68

CPC分类号： G01N33/6896 ,  G01N33/6842 ,  G01N2333/47 ,  G01N2333/4728 ,  G01N2400/40 ,  G01N2800/285 ,  H01J49/0036

摘要： The purpose of the present invention is to develop: a method for selectively separating a glycoprotein derived from the central nervous system from a body fluid or a central nervous system cell; and a method for searching for an index marker for central nervous system diseases, which utilizes the aforementioned method. A protein derived from the central nervous system, which occurs in a trace amount in a body fluid or a central nervous system cell, can be selectively enriched by a two-stage separation procedure comprising removing a glycoprotein having sialic acid at a non-reducing terminal thereof from the body fluid or the central nervous system cell and then separating a glycoprotein having N-acetylglucosamine at a non-reducing terminal thereof.

摘要翻译： 本发明的目的是开发：从体液或中枢神经系统细胞中选择性分离衍生自中枢神经系统的糖蛋白的方法; 以及利用上述方法搜索中枢神经系统疾病指标的方法。 可以通过两步分离方法选择性地富集来自中枢神经系统的蛋白质，其在体液或中枢神经系统细胞中发生微量的蛋白质，其包括在非还原末端除去具有唾液酸的糖蛋白 从体液或中枢神经系统细胞中分离，然后在其非还原末端分离具有N-乙酰葡糖胺的糖蛋白。

公开(公告)号：US20130288272A1

公开(公告)日：2013-10-31

申请号：US13823922

申请日：2011-09-09

申请人： Hisashi Narimatsu ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hiroyuki Kaji ,  Atsushi Kuno ,  Takashi Ohkura ,  Hideki Matsuzaki ,  Yoshitoshi Hirao ,  Jun Iwaki ,  Minako Abe ,  Masaharu Nomura ,  Masayuki Noguchi

发明人： Hisashi Narimatsu ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hiroyuki Kaji ,  Atsushi Kuno ,  Takashi Ohkura ,  Hideki Matsuzaki ,  Yoshitoshi Hirao ,  Jun Iwaki ,  Minako Abe ,  Masaharu Nomura ,  Masayuki Noguchi

IPC分类号： G01N33/68

CPC分类号： G01N33/6893 ,  C07K14/4748 ,  C07K16/28 ,  C07K16/3023 ,  G01N33/574 ,  G01N33/57423 ,  G01N2440/38

摘要： An object of the present invention is to develop and provide a lung cancer differential marker with which lung cancer can be diagnosed conveniently and highly sensitively without depending only on increase or decrease in protein expression level between cancer patients and healthy persons. Another object of the present invention is to develop and provide a glycan marker capable of distinguishing histological types of lung cancer. Of serum glycoproteins, glycopeptide and glycoprotein groups whose glycan structures were altered specifically in lung cancer cell culture supernatants were identified, and they are provided as lung cancer differential markers.

摘要翻译： 本发明的目的是开发和提供肺癌鉴别标记物，其可以方便和高度灵敏地诊断肺癌，而不依赖于癌症患者和健康人之间的蛋白质表达水平的增加或降低。 本发明的另一个目的是开发和提供能够区分肺癌的组织学类型的聚糖标记物。 确定了在肺癌细胞培养上清液中特异性改变其聚糖结构的血清糖蛋白，糖肽和糖蛋白，并将其作为肺癌鉴别标记。

公开(公告)号：US09696320B2

公开(公告)日：2017-07-04

申请号：US13823922

申请日：2011-09-09

申请人： Hisashi Narimatsu ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hiroyuki Kaji ,  Atsushi Kuno ,  Takashi Ohkura ,  Hideki Matsuzaki ,  Yoshitoshi Hirao ,  Jun Iwaki ,  Minako Abe ,  Masaharu Nomura ,  Masayuki Noguchi

发明人： Hisashi Narimatsu ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hiroyuki Kaji ,  Atsushi Kuno ,  Takashi Ohkura ,  Hideki Matsuzaki ,  Yoshitoshi Hirao ,  Jun Iwaki ,  Minako Abe ,  Masaharu Nomura ,  Masayuki Noguchi

IPC分类号： G01N33/574 ,  G01N33/68 ,  C07K14/47 ,  C07K16/28 ,  C07K16/30

CPC分类号： G01N33/6893 ,  C07K14/4748 ,  C07K16/28 ,  C07K16/3023 ,  G01N33/574 ,  G01N33/57423 ,  G01N2440/38

摘要： An object of the present invention is to develop and provide a lung cancer differential marker with which lung cancer can be diagnosed conveniently and highly sensitively without depending only on increase or decrease in protein expression level between cancer patients and healthy persons. Another object of the present invention is to develop and provide a glycan marker capable of distinguishing histological types of lung cancer. Of serum glycoproteins, glycopeptide and glycoprotein groups whose glycan structures were altered specifically in lung cancer cell culture supernatants were identified, and they are provided as lung cancer differential markers.