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    MicroRNA-Based Methods for Prognosis of Hepatocellular Carcinoma
    1.
    发明申请
    MicroRNA-Based Methods for Prognosis of Hepatocellular Carcinoma 审中-公开
    基于微RNA的肝细胞癌预后方法

    公开(公告)号：US20140004521A1

    公开(公告)日：2014-01-02

    申请号：US13539786

    申请日：2012-07-02

    申请人： Chau-Ting Yeh ,  Ya-Hui Huang ,  Hua-Chien Chen ,  Kwang-Huei Lin

    发明人： Chau-Ting Yeh ,  Ya-Hui Huang ,  Hua-Chien Chen ,  Kwang-Huei Lin

    IPC分类号： C12Q1/68 ,  G06F19/10

    CPC分类号： C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/158

    摘要： Disclosed herein is a method for determining the prognosis of a subject diagnosed with hepatocellular carcinoma based on the expression level of at least one microRNA in the non-cancerous liver tissue of the subject. According to various embodiments of the present disclosure, expression levels of miR-486-3p, miR-876-5p, and miR-381 are positively associated with a favorable prognosis, while expression levels of miR-30c, miR-432, and miR-15b are negatively associated with a favorable prognosis.

    摘要翻译： 本文公开了一种基于受试者的非癌性肝组织中至少一种微小RNA的表达水平来确定诊断为肝细胞癌的受试者的预后的方法。 根据本公开的各种实施方案，miR-486-3p，miR-876-5p和miR-381的表达水平与有利的预后正相关，而miR-30c，miR-432和miR的表达水平 -15b与有利的预后呈负相关。

    Treatment and diagnostics of cancer
    2.
    发明申请
    Treatment and diagnostics of cancer 审中-公开
    癌症的治疗和诊断

    公开(公告)号：US20050003405A1

    公开(公告)日：2005-01-06

    申请号：US10833708

    申请日：2004-04-27

    申请人： Hua-Chien Chen ,  Ying Sun ,  Ying-Huey Huang ,  Ming-Chu Hsu ,  Din-Lii Lin

    发明人： Hua-Chien Chen ,  Ying Sun ,  Ying-Huey Huang ,  Ming-Chu Hsu ,  Din-Lii Lin

    IPC分类号： A61K20060101 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/68 ,  G01N33/574

    CPC分类号： C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/16

    摘要： A method of determining whether a subject is suffering from or at risk for developing cancer. The method involves providing a sample from a subject, and determining the level of HM74, LGR6, GPR88, or GPR49 gene expression or protein activity in the sample. The level of HM74, LGR6, GPR88, or GPR49 gene expression or protein activity in the sample, if higher than that in a sample from a normal subject, indicates that the subject is suffering from or at risk for developing cancer. Also disclosed are a method of identifying a compound for treating cancer, a method of treating cancer, and a pharmaceutical composition or a packaged product for treating cancer.

    摘要翻译： 确定受试者是否患有癌症或患癌症风险的方法。 该方法包括从受试者提供样品，并确定样品中HM74，LGR6，GPR88或GPR49基因表达或蛋白质活性的水平。 如果样品中的HM74，LGR6，GPR88或GPR49基因表达或蛋白质活性高于正​​常受试者的样品，则表明该受试者患有癌症或患癌症的风险。 还公开了鉴定用于治疗癌症的化合物的方法，治疗癌症的方法，以及用于治疗癌症的药物组合物或包装产品。

    METHOD FOR DETECTING MULTIPLE SMALL NUCLEIC ACIDS
    4.
    发明申请
    METHOD FOR DETECTING MULTIPLE SMALL NUCLEIC ACIDS 审中-公开
    检测多种小核酸的方法

    公开(公告)号：US20110159482A1

    公开(公告)日：2011-06-30

    申请号：US12823711

    申请日：2010-06-25

    申请人： Po-Ling CHANG ,  Yu-Sun CHANG ,  Shu-Jen CHEN ,  Hua-Chien CHEN

    发明人： Po-Ling CHANG ,  Yu-Sun CHANG ,  Shu-Jen CHEN ,  Hua-Chien CHEN

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6816 ,  C12Q2565/137 ,  C12Q2565/102 ,  C12Q2537/143 ,  C12Q2537/162 ,  C12Q2533/107

    摘要： The present invention discloses a method for simultaneously detecting multiple small nucleic acids, which comprises steps: mixing a specimen, fluorescent probes, and bridge nucleic acids having different lengths to form a tested liquid; hybridizing the mixed short nucleic acid molecules, probes and bridge nucleic acids; adding ligases to enable the ligations of the short nucleic acid molecules and the fluorescent probes with the bridge nucleic acids being the templates; injecting the tested liquid into a capillary, and applying a voltage to the capillary to generate an electrophoresis effect and separate the hybridization products; and using laser to induce different fluorescent rays from different reaction products, and measuring the fluorescent rays, whereby the present invention can simultaneously detect multiple types of short nucleic acid molecules in a single capillary.

    摘要翻译： 本发明公开了一种同时检测多个小核酸的方法，其特征在于包括以下步骤：混合具有不同长度的样品，荧光探针和桥接核酸以形成测试液; 杂交的短核酸分子，探针和桥核酸杂交; 加入连接酶以使短核酸分子和荧光探针与桥核酸作为模板的连接; 将测试液体注入毛细管中，并向毛细管施加电压以产生电泳效应并分离杂交产物; 并使用激光诱导来自不同反应产物的不同荧光，并测量荧光，由此本发明可以在单个毛细管中同时检测多种类型的短核酸分子。

    QUANTITATIVE ANALYSIS METHOD FOR MICRORNAS
    7.
    发明申请
    QUANTITATIVE ANALYSIS METHOD FOR MICRORNAS 审中-公开
    微量定量分析方法

    公开(公告)号：US20100193357A1

    公开(公告)日：2010-08-05

    申请号：US12363133

    申请日：2009-01-30

    申请人： Po-Ling CHANG ,  Yu-Sun CHANG ,  Shu-Jen CHEN ,  Hua-Chien CHEN

    发明人： Po-Ling CHANG ,  Yu-Sun CHANG ,  Shu-Jen CHEN ,  Hua-Chien CHEN

    IPC分类号： G01N27/26 ,  G01N33/00

    CPC分类号： C12Q1/6816 ,  Y10T436/143333 ,  C12Q2565/125 ,  C12Q2545/114 ,  C12Q2525/207

    摘要： The present invention discloses a quantitative analysis method for microRNAs, wherein a fluorescence-labeled DNA probe, which is equinumerous and completely complementary to a microRNA, hybridize with the microRNA. The products of hybridization include the fluorescence-labeled DNA probe containing 22 nucleotides and the probe-microRNA duplex containing 22 base pairs. The products of hybridization is introduced into a capillary by the pressure difference between two ends of the capillary and the siphon effect and separated by electrophoresis. A laser is used to induce fluorescence from the products of hybridization. Then, the intensities of fluorescence are measured and analyzed.

    摘要翻译： 本发明公开了一种微小RNA的定量分析方法，其中与微小RNA同源且完全互补的荧光标记DNA探针与微小RNA杂交。 杂交产物包括含有22个核苷酸的荧光标记的DNA探针和含有22个碱基对的探针 - 微RNA双链体。 杂交产物通过毛细血管两端之间的压力差和虹吸效应引入毛细管，并通过电泳分离。 激光用于诱导杂交产物的荧光。 然后，测量和分析荧光强度。