公开(公告)号：US20110237769A1

公开(公告)日：2011-09-29

申请号：US12459399

申请日：2009-06-30

申请人： Frank J. Feher ,  Gregory M. Whited ,  Gopal K. Chotani ,  Fernando Valle ,  Carol Fioresi ,  Karl J. Sanford ,  Joseph C. McAuliffe ,  Marguerite Cervin ,  Aaron S. Puhala ,  Andrei Miasnikov ,  Ilana S. Aldor

发明人： Frank J. Feher ,  Gregory M. Whited ,  Gopal K. Chotani ,  Fernando Valle ,  Carol Fioresi ,  Karl J. Sanford ,  Joseph C. McAuliffe ,  Marguerite Cervin ,  Aaron S. Puhala ,  Andrei Miasnikov ,  Ilana S. Aldor

IPC分类号： C08F36/08 ,  C08F236/08 ,  C07C11/12 ,  G01N9/00

CPC分类号： C08F236/18 ,  C08F2/00 ,  C08F36/04 ,  C08F136/08 ,  C08F236/08 ,  C08F236/10 ,  C08F236/12 ,  C08L9/00 ,  C12P5/007 ,  Y10T436/24

摘要： It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. In some cases, these cells are cultured in a culture medium that includes a carbon source, such as, but not limited to, a carbohydrate, glycerol, glycerine, dihydroxyacetone, one-carbon source, oil, animal fat, animal oil, fatty acid, lipid, phospholipid, glycerolipid, monoglyceride, diglyceride, triglyceride, renewable carbon source, polypeptide (e.g., a microbial or plant protein or peptide), yeast extract, component from a yeast extract, or any combination of two or more of the foregoing. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. It is anticipated that there will be a significant demand for synthetic rubber and other isoprene containing polymers that are synthesized using isoprene of this type which is made from renewable, non-petrochemical based resources. In fact, it is believed that industrial customers and consumers would prefer to purchase isoprene containing polymers that are derived from such environmentally friendly sources to those that are made with isoprene derived from a petrochemical process. It is further believed that customers would be willing to pay premium prices for such environmentally friendly products that are made with renewable resources. However, it is important to be able to verify that such isoprene containing polymers are actually made from non-petrochemical based resources. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ13C value of greater than −22‰. This type of polyisoprene can be a cis-1,4-polyisoprene homopolymer rubber.

公开(公告)号：US20130122562A1

公开(公告)日：2013-05-16

申请号：US13566916

申请日：2012-08-03

申请人： Ilana S. Aldor ,  Zachary Q. Beck ,  Michael C. Miller ,  Caroline M. Peres

发明人： Ilana S. Aldor ,  Zachary Q. Beck ,  Michael C. Miller ,  Caroline M. Peres

IPC分类号： C12N15/81 ,  C12N15/70

CPC分类号： C12N15/81 ,  C08F36/08 ,  C12N9/1029 ,  C12N9/1085 ,  C12N9/88 ,  C12N15/70 ,  C12P5/007 ,  C12Y203/01194 ,  C12Y402/03027 ,  Y02E50/343 ,  Y02P20/582

摘要： This invention relates to a recombinant microorganism capable of producing isoprene and isoprene production with the use of such recombinant microorganism with good efficiency. In this invention, the acetoacetyl-CoA synthase gene encoding an enzyme capable of synthesizing acetoacetyl-CoA from malonyl-CoA and acetyl-CoA and one or more genes involved in isoprene biosynthesis that enables synthesis of isoprene from acetoacetyl-CoA are introduced into a host microorganism.

摘要翻译： 本发明涉及能够以高效率利用这种重组微生物产生异戊二烯和异戊二烯生产的重组微生物。 在本发明中，将编码能够从丙二酰辅酶A和乙酰辅酶A合成乙酰乙酰辅酶A的酶的乙酰乙酰辅酶A合成酶基因和能够从乙酰乙酰辅酶A合成异戊二烯生物合成的一个或多个基因导入宿主 微生物。

公开(公告)号：US08420759B2

公开(公告)日：2013-04-16

申请号：US12459399

申请日：2009-06-30

申请人： Frank J. Feher ,  Gregory M. Whited ,  Gopal K. Chotani ,  Fernando Valle ,  Carol Fioresi ,  Karl J. Sanford ,  Joseph C. McAuliffe ,  Marguerite Cervin ,  Aaron S. Puhala ,  Andrei Miasnikov ,  Ilana S. Aldor

发明人： Frank J. Feher ,  Gregory M. Whited ,  Gopal K. Chotani ,  Fernando Valle ,  Carol Fioresi ,  Karl J. Sanford ,  Joseph C. McAuliffe ,  Marguerite Cervin ,  Aaron S. Puhala ,  Andrei Miasnikov ,  Ilana S. Aldor

IPC分类号： C08F36/08 ,  G01N33/44 ,  C07C11/18 ,  C12P5/02

CPC分类号： C08F236/18 ,  C08F2/00 ,  C08F36/04 ,  C08F136/08 ,  C08F236/08 ,  C08F236/10 ,  C08F236/12 ,  C08L9/00 ,  C12P5/007 ,  Y10T436/24

摘要： It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ13C value of greater than −22‰.

摘要翻译： 已经发现，培养物中的某些细胞可将大于约0.002％的细胞培养基中可用的碳转化为异戊二烯。 这些细胞具有异源核酸，其（i）编码异戊二烯合酶多肽，和（ii）可操作地连接于启动子。 然后可以回收在这种培养基中产生的异戊二烯并聚合成合成橡胶和其它有用的聚合物材料。 本发明的合成含异戊二烯的聚合物提供可证实为从非石化基础资源得到的益处。 它们也可以分析与来自天然来源的橡胶。 本发明更具体地公开了由衍生自异戊二烯单体的重复单元组成的聚异戊二烯聚合物，其中聚异戊二烯聚合物的δ13C值大于-22‰。

公开(公告)号：US20090203102A1

公开(公告)日：2009-08-13

申请号：US12335071

申请日：2008-12-15

申请人： Marguerite A. Cervin ,  Gregory M. Whited ,  Gopal K. Chotani ,  Fernando Valle ,  Carol Fioresi ,  Karl J. Sanford ,  Joseph C. Mcauliffe ,  Frank J. Feher ,  Aaron S. Puhala ,  Andrei Miasnikov ,  Ilana S. Aldor

发明人： Marguerite A. Cervin ,  Gregory M. Whited ,  Gopal K. Chotani ,  Fernando Valle ,  Carol Fioresi ,  Karl J. Sanford ,  Joseph C. Mcauliffe ,  Frank J. Feher ,  Aaron S. Puhala ,  Andrei Miasnikov ,  Ilana S. Aldor

IPC分类号： C12P5/02 ,  C12N1/20 ,  C12N1/21

CPC分类号： C12P5/007 ,  C12N9/1022 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12Y203/01009 ,  C12Y207/01036 ,  C12Y401/01033 ,  C12Y402/03027 ,  C12Y503/03002 ,  Y02E50/343

摘要： The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.

摘要翻译： 本发明的特征在于从培养细胞产生异戊二烯的方法。 本发明还提供包括这些培养细胞的组合物。

公开(公告)号：US09296850B2

公开(公告)日：2016-03-29

申请号：US14605189

申请日：2015-01-26

申请人： Frank J. Feher ,  Gregory M. Whited ,  Gopal K. Chotani ,  Fernando Valle ,  Carol Fioresi ,  Karl J. Sanford ,  Joseph McAuliffe ,  Marguerite Cervin ,  Aaron S. Puhala ,  Andrei Miasnikov ,  Ilana S. Aldor

发明人： Frank J. Feher ,  Gregory M. Whited ,  Gopal K. Chotani ,  Fernando Valle ,  Carol Fioresi ,  Karl J. Sanford ,  Joseph McAuliffe ,  Marguerite Cervin ,  Aaron S. Puhala ,  Andrei Miasnikov ,  Ilana S. Aldor

IPC分类号： C08F236/18 ,  C08F236/16 ,  C08F236/12 ,  C08F236/10 ,  C08F236/08 ,  C12P5/00 ,  C08F2/00 ,  C08F36/04 ,  C08F136/08 ,  C08L9/00 ,  C12P5/02 ,  C08F4/48

CPC分类号： C08F236/18 ,  C08F2/00 ,  C08F36/04 ,  C08F136/08 ,  C08F236/08 ,  C08F236/10 ,  C08F236/12 ,  C08L9/00 ,  C12P5/007 ,  Y10T436/24

摘要： It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ13C value of greater than −22%.

摘要翻译： 已经发现，培养物中的某些细胞可将大于约0.002％的细胞培养基中可用的碳转化为异戊二烯。 这些细胞具有异源核酸，其（i）编码异戊二烯合酶多肽，和（ii）可操作地连接于启动子。 然后可以回收在这种培养基中产生的异戊二烯并聚合成合成橡胶和其它有用的聚合物材料。 本发明的合成含异戊二烯的聚合物提供可证实为从非石化基础资源得到的益处。 它们也可以分析与来自天然来源的橡胶。 本发明更具体地公开了由衍生自异戊二烯单体的重复单元组成的聚异戊二烯聚合物，其中聚异戊二烯聚合物的δ13C值大于-22％。

公开(公告)号：US20150203620A1

公开(公告)日：2015-07-23

申请号：US14605189

申请日：2015-01-26

申请人： Frank J. Feher ,  Gregory M. Whited ,  Gopal K. Chotani ,  Fernando Valle ,  Carol Fioresi ,  Karl J. Sanford ,  Joseph McAuliffe ,  Marguerite Cervin ,  Aaron S. Puhala ,  Andrei Miasnikov ,  Ilana S. Aldor

发明人： Frank J. Feher ,  Gregory M. Whited ,  Gopal K. Chotani ,  Fernando Valle ,  Carol Fioresi ,  Karl J. Sanford ,  Joseph McAuliffe ,  Marguerite Cervin ,  Aaron S. Puhala ,  Andrei Miasnikov ,  Ilana S. Aldor

IPC分类号： C08F236/18 ,  C08F236/10 ,  C08F236/12 ,  C08F136/08

CPC分类号： C08F236/18 ,  C08F2/00 ,  C08F36/04 ,  C08F136/08 ,  C08F236/08 ,  C08F236/10 ,  C08F236/12 ,  C08L9/00 ,  C12P5/007 ,  Y10T436/24

摘要： It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ13C value of greater than −22%.

摘要翻译： 已经发现，培养物中的某些细胞可将大于约0.002％的细胞培养基中可用的碳转化为异戊二烯。 这些细胞具有异源核酸，其（i）编码异戊二烯合酶多肽，和（ii）可操作地连接于启动子。 然后可以回收在这种培养基中产生的异戊二烯并聚合成合成橡胶和其它有用的聚合物材料。 本发明的合成含异戊二烯的聚合物提供可证实为从非石化基础资源得到的益处。 它们也可以分析与来自天然来源的橡胶。 本发明更具体地公开了由衍生自异戊二烯单体的重复单元组成的聚异戊二烯聚合物，其中聚异戊二烯聚合物的δ13C值大于-22％。