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    Polymers of isoprene from renewable resources
    2.
    发明申请
    Polymers of isoprene from renewable resources 有权

    公开(公告)号：US20110237769A1

    公开(公告)日：2011-09-29

    申请号：US12459399

    申请日：2009-06-30

    申请人： Frank J. Feher ,  Gregory M. Whited ,  Gopal K. Chotani ,  Fernando Valle ,  Carol Fioresi ,  Karl J. Sanford ,  Joseph C. McAuliffe ,  Marguerite Cervin ,  Aaron S. Puhala ,  Andrei Miasnikov ,  Ilana S. Aldor

    发明人： Frank J. Feher ,  Gregory M. Whited ,  Gopal K. Chotani ,  Fernando Valle ,  Carol Fioresi ,  Karl J. Sanford ,  Joseph C. McAuliffe ,  Marguerite Cervin ,  Aaron S. Puhala ,  Andrei Miasnikov ,  Ilana S. Aldor

    IPC分类号： C08F36/08 ,  C08F236/08 ,  C07C11/12 ,  G01N9/00

    CPC分类号： C08F236/18 ,  C08F2/00 ,  C08F36/04 ,  C08F136/08 ,  C08F236/08 ,  C08F236/10 ,  C08F236/12 ,  C08L9/00 ,  C12P5/007 ,  Y10T436/24

    摘要： It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. In some cases, these cells are cultured in a culture medium that includes a carbon source, such as, but not limited to, a carbohydrate, glycerol, glycerine, dihydroxyacetone, one-carbon source, oil, animal fat, animal oil, fatty acid, lipid, phospholipid, glycerolipid, monoglyceride, diglyceride, triglyceride, renewable carbon source, polypeptide (e.g., a microbial or plant protein or peptide), yeast extract, component from a yeast extract, or any combination of two or more of the foregoing. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. It is anticipated that there will be a significant demand for synthetic rubber and other isoprene containing polymers that are synthesized using isoprene of this type which is made from renewable, non-petrochemical based resources. In fact, it is believed that industrial customers and consumers would prefer to purchase isoprene containing polymers that are derived from such environmentally friendly sources to those that are made with isoprene derived from a petrochemical process. It is further believed that customers would be willing to pay premium prices for such environmentally friendly products that are made with renewable resources. However, it is important to be able to verify that such isoprene containing polymers are actually made from non-petrochemical based resources. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ13C value of greater than −22‰. This type of polyisoprene can be a cis-1,4-polyisoprene homopolymer rubber.

    Proteases with modified pro regions
    9.
    发明授权
    Proteases with modified pro regions 有权

    公开(公告)号：US08530218B2

    公开(公告)日：2013-09-10

    申请号：US12761275

    申请日：2010-04-15

    申请人： Eugenio Ferrari ,  Carol Fioresi ,  Anita van Kimmenade

    发明人： Eugenio Ferrari ,  Carol Fioresi ,  Anita van Kimmenade

    IPC分类号： C12N9/00 ,  C12N9/54 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12N9/54 ,  C12N15/75

    摘要： The present invention provides methods and compositions for the production of mature proteases in bacterial host cells. The compositions include modified polynucleotides that encode modified proteases, which have at least one mutation in the pro region; the modified serine proteases encoded by the modified polynucleotides; expression cassettes, DNA constructs, and vectors comprising the modified polynucleotides that encode the modified proteases; and the bacterial host cells transformed with the vectors of the invention. The methods include methods for enhancing the production of mature proteases in bacterial host cells e.g. Bacillus sp. host cells. The produced proteases find use in the industrial production of enzymes, suitable for use in various industries, including but not limited to the cleaning, animal feed and textile processing industry.