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1.发明授权Caffeine fraction obtained from tea leaves and a method for inducing Agrobacterium tumefaciens-mediated genetic transformation in plants using said caffeine fraction 失效从茶叶获得的咖啡因馏分和使用所述咖啡因馏分在植物中诱导根癌土壤杆菌介导的遗传转化的方法公开(公告)号：US07879371B2
公开(公告)日：2011-02-01
申请号：US12568502
申请日：2009-09-28
申请人： Indra Sandal , Ajay Kumar , Amita Bhattacharya , Ravindranath Srigiripuram Desikalhar , Ashu Gulati , Paramvir Singh Ahuja
发明人： Indra Sandal , Ajay Kumar , Amita Bhattacharya , Ravindranath Srigiripuram Desikalhar , Ashu Gulati , Paramvir Singh Ahuja
IPC分类号： A61K36/82
CPC分类号： C12N15/8205 , C07D473/12
摘要： The present invention relates to a thermolabile caffeine fraction useful for an efficient Agrobacterium-mediated genetic transformation in plant systems to develop desired traits in plant, and a method of preparing said fraction from tea leaves and also, an efficient and cost-effective method of introducing said Agrobacterium-mediated genetic transformation into plant systems using said caffeine fraction of tea leaves.
摘要翻译： 本发明涉及可用于植物系统中有效的农杆菌介导的遗传转化以产生植物中期望的性状的不热咖啡因级分，以及从茶叶中制备所述级分的方法，以及一种有效和成本有效的引入方法 所述农杆菌介导的遗传转化到植物系统中，使用茶叶的咖啡因级分。
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2.发明授权公开(公告)号：US07145058B2
公开(公告)日：2006-12-05
申请号：US10106528
申请日：2002-03-27
申请人： Indra Sandal , Amita Bhattacharya , Ashu Gulati , Srigiripuram D. Ravindranath , Paramvir Singh Ahuja
发明人： Indra Sandal , Amita Bhattacharya , Ashu Gulati , Srigiripuram D. Ravindranath , Paramvir Singh Ahuja
CPC分类号： C12N15/8205 , A01N65/08
摘要： The present invention relates to an efficient and cost effective method of preventing growth of genetic transformant bacteria Agrobacterium tumefaciens after transformation in plants by using tea leaf extract as a bactericide, wherein said method leads to elimination of common problem of polyphenol oxidation during transformation and thereby helps maintain regeneration potential in explants and also helps in increased transformation efficacy
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公开(公告)号：US06599743B2
公开(公告)日：2003-07-29
申请号：US09813101
申请日：2001-03-22
申请人： Indra Sandal , Amita Bhattacharya , Madhu Sharma , P. S. Ahuja
发明人： Indra Sandal , Amita Bhattacharya , Madhu Sharma , P. S. Ahuja
IPC分类号： C12N500
CPC分类号： C12N5/04
摘要： The present invention relates to novel method for micropropagation of tea plants from explants obtained from completely folded, half opened or fully expanded leaves via callus phase, by culturing the explants on different media.
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4.发明授权Caffeine fraction obtained from tea leaves and a method for inducing Agrobacterium tumefaciens-mediated genetic transformation in plants using said caffeine fraction 失效从茶叶获得的咖啡因馏分和使用所述咖啡因馏分在植物中诱导根癌土壤杆菌介导的遗传转化的方法公开(公告)号：US07608288B2
公开(公告)日：2009-10-27
申请号：US10396593
申请日：2003-03-25
申请人： Indra Sandal , Ajay Kumar , Amita Bhattacharya , Ravindranath Srigiripuram Desikalhar , Ashu Gulati , Paramvir Singh Ahuja
发明人： Indra Sandal , Ajay Kumar , Amita Bhattacharya , Ravindranath Srigiripuram Desikalhar , Ashu Gulati , Paramvir Singh Ahuja
IPC分类号： A61K36/82 , C07D473/00
CPC分类号： C12N15/8205 , C07D473/12
摘要： The present invention relates to a thermolabile caffeine fraction useful for an efficient Agrobacterium-mediated genetic transformation in plant systems to develop desired traits in plants, and a method of preparing said fraction from tea leaves and also, an efficient and cost-effective method of introducing said Agrobacterium-mediated genetic transformation into plant systems using said caffeine fraction of tea leaves.
摘要翻译： 本发明涉及可用于植物系统中有效的农杆菌介导的遗传转化以产生植物中期望的性状的不热咖啡因级分，以及从茶叶制备所述级分的方法，以及一种有效和成本有效的引入方法 所述农杆菌介导的遗传转化到植物系统中，使用茶叶的咖啡因级分。
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5.发明申请CAFFEINE FRACTION OBTAINED FROM TEA LEAVES AND A METHOD FOR INDUCING AGROBACTERIUM TUMEFACIENS-MEDIATED GENETIC TRANSFORMATION IN PLANTS USING SAID CAFFEINE FRACTION 审中-公开从茶叶获得的CAFFEINE分离物和用于诱导植物中的农杆菌介导的遗传转化的方法使用合适的CAFFEINE分段公开(公告)号：US20100077511A1
公开(公告)日：2010-03-25
申请号：US12562620
申请日：2009-09-18
申请人： Indra SANDAL , Ajay Kumar , Amita Bhattacharya , Ravindranath Srigiripuram Desikalhar , Ashu Gulati , Paramvir Singh Ahuja
发明人： Indra SANDAL , Ajay Kumar , Amita Bhattacharya , Ravindranath Srigiripuram Desikalhar , Ashu Gulati , Paramvir Singh Ahuja
IPC分类号： C12N15/87
CPC分类号： C07D473/12 , C12N15/8205
摘要： The present invention relates to a thermolabile caffeine fraction useful for an efficient Agrobacterium-mediated genetic transformation in plant systems to develop desired traits in plant, and a method of preparing said fraction from tea leaves and also, an efficient and cost-effective method of introducing said Agrobacterium-mediated genetic transformation into plant systems using said caffeine fraction of tea leaves.
摘要翻译： 本发明涉及可用于植物系统中有效的农杆菌介导的遗传转化以产生植物中期望的性状的热不定咖啡因级分，以及从茶叶中制备所述级分的方法，以及一种有效和成本有效的引入方法 所述农杆菌介导的遗传转化到植物系统中，使用茶叶的咖啡因级分。
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公开(公告)号：US07129394B2
公开(公告)日：2006-10-31
申请号：US10051383
申请日：2002-01-22
CPC分类号： C12N15/8207
摘要： The present invention relates to Production of transgenic tea (Camellia sinensis (L.) O. Kuntze) through biolistic.
摘要翻译： 本发明涉及通过生物实验生产转基因茶（Camellia sinensis（L.）O.Kuntze）。
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公开(公告)号：US20100016588A1
公开(公告)日：2010-01-21
申请号：US12568502
申请日：2009-09-28
申请人： Indra SANDAL , Ajay Kumar , Amita Bhattacharya , Ravindranath Srigiripuram Desikalhar , Ashu Gulati , Paramvir Singh Ahuja
发明人： Indra SANDAL , Ajay Kumar , Amita Bhattacharya , Ravindranath Srigiripuram Desikalhar , Ashu Gulati , Paramvir Singh Ahuja
IPC分类号： C07D473/08
CPC分类号： C12N15/8205 , C07D473/12
摘要： The present invention relates to a thermolabile caffeine fraction useful for an efficient Agrobacterium-mediated genetic transformation in plant systems to develop desired traits in plant, and a method of preparing said fraction from tea leaves and also, an efficient and cost-effective method of introducing said Agrobacterium-mediated genetic transformation into plant systems using said caffeine fraction of tea leaves.
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公开(公告)号：US06548300B1
公开(公告)日：2003-04-15
申请号：US09811777
申请日：2001-03-20
IPC分类号： C12N500
摘要： The invention provides a one step method for micropropagation of tea plants from leaf explants via callus phase, wherein the explants are maintained in basal Murashige and Skoog medium (Murashige T. and Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497; 1962) supplemented with vitamins like thiamine-HCl (0.05 to 2.0 mg/l), pyridoxine-HCl (0.25 to 1.5 mg/l) and nicotinic acid (0.25 to 1.5 mg/l) together with glycine (1.0 to 3.0 mg/l) and either 2.5, 5.0 or 7.5 mg/l 2,4-Dichlorophenoxy acetic acid for a period of 14-16 weeks, from the stage of induction of callus to rhizogenesis and shoot formation.
摘要翻译： 本发明提供了一种通过愈伤组织从叶片外植体中微量繁殖茶叶的方法，其中将外植体保持在基底Murashige和Skoog培养基中（Murashige T.和Skoog F.用于快速生长和用烟草组织培养物进行生物测定的修订培养基 补充维生素如硫胺素-HCl（0.05-2.0mg / l），吡哆醇-HCl（0.25-1.5mg / l）和烟酸（0.25-1.5mg / l）的生理工厂15：473-497; 1962） ）与甘氨酸（1.0-3.0mg / l）和2.5,5.0或7.5mg / l 2,4-二氯苯氧基乙酸一起，持续14-16周，从诱导愈伤组织到发根和芽形成阶段 。
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