Biosensor and Method of Manufacturing Such a Biosensor
    4.
    发明申请
    Biosensor and Method of Manufacturing Such a Biosensor 有权
    生物传感器和制造这种生物传感器的方法

    公开(公告)号:US20130327649A1

    公开(公告)日:2013-12-12

    申请号:US14000957

    申请日:2012-02-14

    IPC分类号: G01N27/327

    摘要: A method of manufacturing a biosensor having a microbeam linked to a support, at least one electrode a biological molecule A grafted onto the microbeam in a different zone from the zone where the electrode is embedded, and a mechanoelectrical transducer for converting variations of the mechanical properties of the microbeam into an electrical signal, when the biological molecule A is placed in contact with a biological molecule B to be detected and/or quantified. The method includes: formation of an electrode on fluoropolymer material sheet, passivation of the electrode(s), creation of the form of the biosensor in the sheet of polymer material and separation of this form from the sheet, functionalization either of a prefunctionalized zone or of a zone of the microbeam, this zone being different from the zone wherein the electrode is embedded, and grafting of a biological molecule A onto the functionalized zone.

    摘要翻译: 一种制造具有与载体连接的微束的生物传感器的方法,至少一个电极,在与嵌入电极的区域不同的区域中接枝到微束上的生物分子A和用于转换机械性能变化的机械电传感器 当将生物分子A与待检测和/或定量的生物分子B接触时,将微束束转化为电信号。 该方法包括:在含氟聚合物材料片上形成电极,使电极钝化,在聚合物材料片中形成生物传感器的形式并将该形式与片材分离,将功能化前的功能化区域 的微波束的区域,该区域不同于电极被嵌入的区域,并且将生物分子A接枝到官能化区域上。

    Probe arrangement
    5.
    发明授权
    Probe arrangement 有权
    探测安排

    公开(公告)号:US07870616B2

    公开(公告)日:2011-01-11

    申请号:US12117801

    申请日:2008-05-09

    IPC分类号: H01J37/252

    摘要: A probe arrangement with a probe for local electrophysiological analysis of cells (4) such as patch-clamp techniques for use with atomic force microscopy, has a probe with a cantilever arm (2) connected to a probe holder (3). The probe has a probe tip (4) at a probing end (5) of the cantilever arm (2) and a fluid channel (6) in the cantilever arm (2) connecting a probe tip aperture (7) with a fluid reservoir (8) via a duct (9). The fluid channel (6), the duct (9) and the fluid reservoir (8) are adapted to be filled with a fluid solution (10) enabling ion transport for electrophysiological analysis. A first electrode (15) for electrophysiological analysis is placed in the fluid reservoir (8) and/or in the duct (9) and/or in the fluid channel (6).

    摘要翻译: 具有用于细胞局部电生理分析的探针的探针装置(4),例如用原子力显微镜使用的膜片钳技术,具有连接到探针架(3)的悬臂(2)的探针。 探针在悬臂(2)的探测端(5)处具有探针尖端(4)以及悬臂(2)中的流体通道(6),其将探针尖端孔(7)与流体储存器 8)通过管道(9)。 流体通道(6),管道(9)和流体储存器(8)适于填充有能够进行电生理分析的离子输送的流体溶液(10)。 用于电生理分析的第一电极(15)放置在流体储存器(8)和/或管道(9)和/或流体通道(6)中。