公开(公告)号：US06319379B1

公开(公告)日：2001-11-20

申请号：US09379378

申请日：1999-08-23

申请人： J. Courtney Davidson ,  Joseph W. Balch

发明人： J. Courtney Davidson ,  Joseph W. Balch

IPC分类号： G01N2726

CPC分类号： G01N30/16 ,  G01N27/44743 ,  G01N30/6095 ,  G01N2030/285 ,  G01N2030/562 ,  G01N30/466

摘要： A sample injection method for horizontal configured multiple chromatography or electrophoresis units, each containing a number of separation/analysis channels, that enables efficient introduction of analyte samples. This method for loading when taken in conjunction with horizontal microchannels allows much reduced sample volumes and a means of sample stacking to greatly reduce the concentration of the sample. This reduction in the amount of sample can lead to great cost savings in sample preparation, particularly in massively parallel applications such as DNA sequencing. The essence of this method is in preparation of the input of the separation channel, the physical sample introduction, and subsequent removal of excess material. By this method, sample volumes of 100 nanoliter to 2 microliters have been used successfully, compared to the typical 5 microliters of sample required by the prior separation/analysis method.

摘要翻译： 用于水平配置的多个色谱或电泳单元的样品注射方法，每个包含多个分离/分析通道，能够有效地引入分析物样品。 当与水平微通道结合使用时，这种加载方法允许大大减少样品体积和样品堆叠的手段，以大大降低样品的浓度。 样品量的减少可以在样品制备中节省大量成本，特别是在大规模并行应用如DNA测序中。 该方法的本质在于准备分离通道的输入，物理样品引入和随后的多余材料的去除。 通过这种方法，与以前的分离/分析方法所需的典型5微升样品相比，成功地使用了100纳升至2微升的样品体积。

公开(公告)号：US06225635B1

公开(公告)日：2001-05-01

申请号：US09131183

申请日：1998-08-07

申请人： Laurence R. Brewer ,  Joseph Kimbrough ,  Joseph Balch ,  J. Courtney Davidson

发明人： Laurence R. Brewer ,  Joseph Kimbrough ,  Joseph Balch ,  J. Courtney Davidson

IPC分类号： G01N27447

CPC分类号： G01N27/44721

摘要： A system and method is disclosed for optically locating a microchannel position. A laser source generates a primary laser beam which is directed at a microchannel plate. The microchannel plates include microchannels at various locations. A back-reflectance beam detector receives a back-reflected beam from the plate. The back-reflected beam is generated when the primary beam reflects off of the plate. A photodiode circuit generates a trigger signal when the back-reflected beam exceeds a predetermined threshold, indicating a presence of the microchannel. The method of the present invention includes the steps of generating a primary beam, directing the primary beam to a plate containing a microchannel, receiving from the plate a back-reflected beam generated in response to the primary beam, and generating a trigger signal when the back-reflected beam exceeds a predetermined threshold which corresponds to a presence of the microchannel.

摘要翻译： 公开了用于光学定位微通道位置的系统和方法。 激光源产生指向微通道板的主激光束。 微通道板包括在各个位置的微通道。 后反射光束检测器从板接收反向反射光束。 当主光束反射离开板时，产生反射反射光束。 当反射光束超过预定阈值时，光电二极管电路产生触发信号，指示存在微通道。 本发明的方法包括以下步骤：产生主光束，将主光束引导到包含微通道的光盘;从该光盘接收响应于主光束产生的反射反射光束，并在 反向反射光束超过对应于微通道存在的预定阈值。

公开(公告)号：US07036220B2

公开(公告)日：2006-05-02

申请号：US10742112

申请日：2003-12-18

申请人： J. Courtney Davidson ,  Peter A. Krulevitch ,  Mariam N. Maghribi ,  Julie K. Hamilton ,  William J. Benett ,  Armando R. Tovar

发明人： J. Courtney Davidson ,  Peter A. Krulevitch ,  Mariam N. Maghribi ,  Julie K. Hamilton ,  William J. Benett ,  Armando R. Tovar

IPC分类号： H05K3/10

CPC分类号： H01L21/76838 ,  H05K3/4053 ,  H05K2203/0195 ,  H05K2203/0338 ,  Y10T29/49155

摘要： A method of metalization of an integrated microsystem. The method comprises providing a substrate and applying a conductive material to the substrate by taking up small aliquots of conductive material and releasing the conductive material onto the substrate to produce a circuit component.

摘要翻译： 一种集成微系统的金属化方法。 该方法包括提供衬底并且通过吸收导电材料的小等分试样并将导电材料释放到衬底上以产生电路部件来向衬底施加导电材料。