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公开(公告)号：US5891636A
公开(公告)日：1999-04-06
申请号：US922965
申请日：1997-09-03
CPC分类号： C12N15/1096 , C12Q1/6865
摘要： This invention relates to the use of promoters for ribonucleic acid amplification and other genetic manipulations. Processes are provided wherein complementary deoxyribonucleic acid (cDNA) is synthesized from a ribonucleic acid (RNA) sequence using a complementary primer linked to an RNA polymerase promoter region complement and then anti-sense RNA (aRNA) is transcribed from the cDNA by introducing an RNA polymerase capable of binding to the promoter region. Additional processes using the resulting aRNA are also described.
摘要翻译： 本发明涉及用于核糖核酸扩增和其他遗传操作的启动子的用途。 提供了使用与RNA聚合酶启动子区域补体连接的互补引物从核糖核酸（RNA）序列合成互补脱氧核糖核酸（cDNA）的方法，然后通过引入RNA从cDNA转录反义RNA（aRNA） 能够结合启动子区域的聚合酶。 还描述了使用所得的aRNA的另外的方法。
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2.发明授权Process for amplifying a target polynucleotide sequence using a single primer-promoter complex 失效使用单一引物启动子复合物扩增靶多核苷酸序列的方法
公开(公告)号：US5545522A
公开(公告)日：1996-08-13
申请号：US957647
申请日：1992-10-05
CPC分类号： C12N15/1096 , C12Q1/6865
摘要： This invention relates to the use of promoters for ribonucleic acid amplification and other genetic manipulations. Processes are provided wherein complementary deoxyribonucleic acid (cDNA) is synthesized from a ribonucleic acid (RNA) sequence using a complementary primer linked to an RNA polymerase promoter region complement and then anti-sense RNA (aRNA) is transcribed from the cDNA by introducing an RNA polymerase capable of binding to the promoter region. Additional processes using the resulting aRNA are also described.
摘要翻译： 本发明涉及用于核糖核酸扩增和其他遗传操作的启动子的用途。 提供了使用与RNA聚合酶启动子区域补体连接的互补引物从核糖核酸（RNA）序列合成互补脱氧核糖核酸（cDNA）的方法，然后通过引入RNA从cDNA转录反义RNA（aRNA） 能够结合启动子区域的聚合酶。 还描述了使用所得的aRNA的另外的方法。
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3.发明授权Method of suppressing appetite by administration of tetrahydro-beta-carboline derivatives 失效通过给予四氢-β-咔啉衍生物抑制食欲的方法
公开(公告)号：US5169852A
公开(公告)日：1992-12-08
申请号：US628692
申请日：1990-12-14
申请人： Jack D. Barchas , Glen R. Elliott , Peter I. Adriaenssens , Robert S. Bitner , Stephen S. Bowersox
发明人： Jack D. Barchas , Glen R. Elliott , Peter I. Adriaenssens , Robert S. Bitner , Stephen S. Bowersox
IPC分类号： A61K31/44
CPC分类号： A61K31/44 , Y10S514/909
摘要： Compounds of formula I are useful for suppressing appetite, and for altering macronutrient preferences, ##STR1## where the R.sub.2 -R.sub.9 substituents are so selected as to meet a defined quantitative structure activity relationship (QSAR) quantity relating to the partition coefficient of the compound, the net charge on the ring nitrogen, and the radical superfdelocalizability at the position-8 ring carbon.
摘要翻译： 式I的化合物可用于抑制食欲，并且用于改变大量营养素喜好
，其中选择R2-R9取代基以满足与化合物的分配系数有关的定义的定量结构活性关系（QSAR） 环氮的净电荷，以及位置-8环碳的自由基超定位。 -
公开(公告)号：US4613586A
公开(公告)日：1986-09-23
申请号：US602116
申请日：1984-04-19
申请人： Jack D. Barchas , Eckard Weber , Christopher J. Evans , Jaw-Kang Chang , Robin G. Lorenz , Kevin A. Roth
发明人： Jack D. Barchas , Eckard Weber , Christopher J. Evans , Jaw-Kang Chang , Robin G. Lorenz , Kevin A. Roth
IPC分类号： A61K38/00 , C07K14/575 , C07K7/06 , C07K7/08
CPC分类号： C07K14/57572 , A61K38/00
摘要： Novel peptides having gastrin releasing peptide-like activity having the structures Ala-Pro-Val-Ser-Val-Gly-Gly-Gly-Thr-Val-Leu-Ala-Lys-Met-Tyr-Pro-NH.sub.2 and Gly-Asn-His-Trp-Ala-Val-Gly-His-Leu-Met-NH.sub.2, have been isolated from rat and guinea pig brain extracts and synthesized by a solid phase peptide synthesis methods. The GRP-like peptides are useful as pharmaceuticals to lower body temperature, increase respiratory activity, reduce food intake, stimulate pancreatic hormone release, stimulate pituitary hormone release and suppress thyroid hormone release in mammals.
摘要翻译： 具有胃泌素释放肽样活性的具有Ala-Pro-Val-Ser-Val-Gly-Gly-Gly-Thr-Val-Leu-Ala-Lys-Met-Tyr-Pro-NH2和Gly-Asn- His-Trp-Ala-Val-Gly-His-Leu-Met-NH2已经从大鼠和豚鼠脑提取物中分离并通过固相肽合成方法合成。 GRP样肽可用作降低体温，增加呼吸活动，减少食物摄取，刺激胰腺激素释放，刺激垂体激素释放和抑制哺乳动物中甲状腺激素释放的药物。
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公开(公告)号：US5716785A
公开(公告)日：1998-02-10
申请号：US636748
申请日：1996-04-19
CPC分类号： C12N15/1096 , C12Q1/6865
摘要： This invention relates to the use of promoters for ribonucleic acid amplification and other genetic manipulations. Processes are provided wherein complementary deoxyribonucleic acid (cDNA) is synthesized from a ribonucleic acid (RNA) sequence using a complementary primer linked to an RNA polymerase promoter region complement and then anti-sense RNA (aRNA) is transcribed from the cDNA by introducing an RNA polymerase capable of binding to the promoter region. Additional processes using the resulting aRNA are also described.
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公开(公告)号：US5021335A
公开(公告)日：1991-06-04
申请号：US208267
申请日：1988-06-17
申请人： Laurence Tecott , Jack D. Barchas , James Eberwine
发明人： Laurence Tecott , Jack D. Barchas , James Eberwine
CPC分类号： C12Q1/6841 , C12Q1/48
摘要： In situ transcription is provided by hybridizing cells or tissue with a primer, and extending the primer bound to any template mRNA or DNA with reverse transcriptase or DNA polymerase in the presence of labeled nucleotides, which allows for detection of cells containing the template. The resulting cDNA may be eluted and used in a polymerase chain reaction for isolation of the DNA, and/or the cDNA electrophoresed, which may provide for information concerning the sequence, or the like.
摘要翻译： 通过用引物杂交细胞或组织提供原位转录，并且在标记的核苷酸存在下扩增与逆转录酶或DNA聚合酶结合到任何模板mRNA或DNA的引物，其允许检测含有该模板的细胞。 所得的cDNA可以被洗脱并用于聚合酶链式反应以分离DNA，和/或电泳的cDNA，其可以提供关于序列的信息等。
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7.发明授权Method of suppressing appetite by administration of tetrahydro-beta-carboline derivatives 失效通过给予四氢-β-咔啉衍生物抑制食欲的方法
公开(公告)号：US4978669A
公开(公告)日：1990-12-18
申请号：US363504
申请日：1989-06-08
申请人： Jack D. Barchas , Glen R. Elliott , Peter I. Adriaenssens , Robert S. Bitner , Stephen S. Bowersox , Laszlo Nadasdi
发明人： Jack D. Barchas , Glen R. Elliott , Peter I. Adriaenssens , Robert S. Bitner , Stephen S. Bowersox , Laszlo Nadasdi
IPC分类号： A61K31/435 , A61K31/44 , A61P3/04 , C07D471/04
CPC分类号： A61K31/44 , Y10S514/909
摘要： Compounds of formula I are useful for suppressing appetite, for altering macronutrient preferences, for suppressing obsessive-compulsive behavior, and for inhibiting cravings and substance abuse: ##STR1## wherein R.sub.1 and R.sub.3 are each independently H, hydroxy-alkyl, alpha-cyanoalkyl, SO.sub.3 H, SO.sub.2 NH.sub.2, or C(O)R, where R is OH, NH.sub.2, lower alkoxy, benzyloxy, or aliphatic amino acyl;R.sub.2 and R.sub.9 are each independently H, lower alkyl, benzyl, succinyl, or C(O)R.sub.4, where R.sub.4 is H, lower alkyl, hydrocarboxy-lower alkylene, or lower alkoxycarboxy-lower alkylene; andR.sub.5, R.sub.6, R.sub.7, and R.sub.8 are each independently H, halo, lower alkyl, hydroxy, lower alkoxy, or two adjacent radicals form methylenedioxy or ethylenedioxy;with the proviso that R.sub.1, R.sub.2, R.sub.3, R.sub.5, R.sub.6, R.sub.7, R.sub.8, and R.sub.9 are not simultaneously H.
摘要翻译： 式I的化合物可用于抑制食欲，改变大量营养素偏好，抑制强迫症行为和抑制欲望和物质滥用：其中R1和R3各自独立地为H，羟基 - 烷基，α 氰基烷基，SO 3 H，SO 2 NH 2或C（O）R，其中R是OH，NH 2，低级烷氧基，苄氧基或脂族氨基酰基; R 2和R 9各自独立地为H，低级烷基，苄基，琥珀酰基或C（O）R 4，其中R 4为H，低级烷基，烃基 - 低级亚烷基或低级烷氧基羧基 - 低级亚烷基; 并且R 5，R 6，R 7和R 8各自独立地为H，卤素，低级烷基，羟基，低级烷氧基或两个相邻基团形成亚甲二氧基或亚乙二氧基; 条件是R1，R2，R3，R5，R6，R7，R8和R9不同时为H.
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公开(公告)号：US07049102B1
公开(公告)日：2006-05-23
申请号：US09713545
申请日：2000-11-15
CPC分类号： C12P19/34 , C12Q1/6809 , C12Q1/6865 , C12Q2531/143 , C12Q2537/143
摘要： This invention relates to the use of promoters for ribonucleic acid amplification and other genetic manipulations. Processes are provided wherein complementary deoxyribonucleic acid (cDNA) is synthesized from a ribonucleic acid (RNA) sequence using a complementary primer linked to an RNA polymerase promoter region complement and then anti-sense RNA (aRNA) is transcribed from the cDNA by introducing an RNA polymerase capable of binding to the promoter region. Additional processes using the resulting aRNA are also described.
摘要翻译： 本发明涉及用于核糖核酸扩增和其他遗传操作的启动子的用途。 提供了使用与RNA聚合酶启动子区域补体连接的互补引物从核糖核酸（RNA）序列合成互补脱氧核糖核酸（cDNA）的方法，然后通过引入RNA从cDNA转录反义RNA（aRNA） 能够结合启动子区域的聚合酶。 还描述了使用所得的aRNA的另外的方法。
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公开(公告)号：US06291170B1
公开(公告)日：2001-09-18
申请号：US09286533
申请日：1999-04-05
IPC分类号： C12Q168
CPC分类号： C12N15/1096 , C12Q1/6865
摘要： This invention relates to the use of promoters for ribonucleic acid amplification and other genetic manipulations. Processes are provided wherein complementary deoxyribonucleic acid (cDNA) is synthesized from a ribonucleic acid (RNA) sequence using a complementary primer linked to an RNA polymerase promoter region complement and then anti-sense RNA (aRNA) is transcribed from the cDNA by introducing an RNA polymerase capable of binding to the promoter region. Additional processes using the resulting aRNA are also described.
摘要翻译： 本发明涉及用于核糖核酸扩增和其他遗传操作的启动子的用途。 提供了使用与RNA聚合酶启动子区域补体连接的互补引物从核糖核酸（RNA）序列合成互补脱氧核糖核酸（cDNA）的方法，然后通过引入RNA从cDNA转录反义RNA（aRNA） 能够结合启动子区域的聚合酶。 还描述了使用所得的aRNA的另外的方法。
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公开(公告)号：US5168038A
公开(公告)日：1992-12-01
申请号：US671113
申请日：1991-03-18
申请人： Laurence Tecott , Jack D. Barchas , James Eberwine
发明人： Laurence Tecott , Jack D. Barchas , James Eberwine
CPC分类号： C12Q1/48 , C12Q1/6841
摘要： In situ transcription is provided by hybridizing cells or tissue with a primer, and extending the primer bound to any template mRNA or DNA with transverse transcriptase or DNA polymerase in the presence of labeled nucleotides, which allows for detection of cells containing the template. The resulting cDNA may be eluted and used in a polymerase chain reaction for isolation of the DNA, and/or the cDNA electrophoresed, which may provide for information concerning the sequence, or the like.
摘要翻译： 通过用引物杂交细胞或组织提供原位转录，并且在标记的核苷酸存在下扩增与横向转录酶或DNA聚合酶连接的任何模板mRNA或DNA的引物，其允许检测含有该模板的细胞。 所得的cDNA可以被洗脱并用于聚合酶链式反应以分离DNA，和/或电泳的cDNA，其可以提供关于序列的信息等。
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