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公开(公告)号：US07771945B2
公开(公告)日：2010-08-10
申请号：US11965607
申请日：2007-12-27
CPC分类号： C12N15/101 , B03D3/06 , C12N1/066 , C12N15/1003 , C12N15/1017 , C12Q1/6806 , C12Q2527/119 , C12Q2565/137
摘要： Apparatus and methods are described for pharmaceutical grade manufacture extrachromosomal nucleic acids from cell lysates using flotation to separate and eliminate undesired insoluble cellular debris including chromosomal DNA from the lysates. A gas is introduced to controllably generate bubbles that reduce the density of the cell debris and create a buoyant flocculent phase that can be readily separated from, and thus provide, a substantially clarified fluid lysate phase that is enriched in extrachromosomal DNA but substantially depleted of cellular proteins and chromosomal DNA.
摘要翻译： 对来自细胞裂解物的药物级制造染色体外核酸描述了装置和方法，使用浮选来分离和消除包括来自裂解物的染色体DNA的不期望的不溶性细胞碎片。 引入气体以可控制地产生气泡，其降低细胞碎片的密度并产生浮力絮状相，其可以容易地从基质上澄清的流体裂解物相分离，并且因此提供富含染色体外DNA但基本上耗尽细胞的基本上澄清的流体裂解物相 蛋白质和染色体DNA。
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公开(公告)号：US20080102519A1
公开(公告)日：2008-05-01
申请号：US11965607
申请日：2007-12-27
IPC分类号： C12M1/04
CPC分类号： C12N15/101 , B03D3/06 , C12N1/066 , C12N15/1003 , C12N15/1017 , C12Q1/6806 , C12Q2527/119 , C12Q2565/137
摘要： Apparatus and methods are described for pharmaceutical grade manufacture extrachromosomal nucleic acids from cell lysates using flotation to separate and eliminate undesired insoluble cellular debris including chromosomal DNA from the lysates. A gas is introduced to controllably generate bubbles that reduce the density of the cell debris and create a buoyant flocculent phase that can be readily separated from, and thus provide, a substantially clarified fluid lysate phase that is enriched in extrachromosomal DNA but substantially depleted of cellular proteins and chromosomal DNA.
摘要翻译： 对来自细胞裂解物的药物级制造染色体外核酸描述了装置和方法，使用浮选来分离和消除包括来自裂解物的染色体DNA的不期望的不溶性细胞碎片。 引入气体以可控制地产生气泡，其降低细胞碎片的密度并产生浮力絮状相，其可以容易地从基质上澄清的流体裂解物相分离，并且因此提供富含染色体外DNA但基本上耗尽细胞的基本上澄清的流体裂解物相 蛋白质和染色体DNA。
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公开(公告)号：US20070190101A1
公开(公告)日：2007-08-16
申请号：US10815000
申请日：2004-03-31
申请人： Chunlin Yang , Jacky Au-Yeung , Iksoo Chun , Mark Davis , Lu Liu , Aruna Nathan , Vivek Shenoy , Mark Timmer
发明人： Chunlin Yang , Jacky Au-Yeung , Iksoo Chun , Mark Davis , Lu Liu , Aruna Nathan , Vivek Shenoy , Mark Timmer
CPC分类号： A61K38/39 , A61K35/28 , A61K38/00 , A61L27/26 , A61L27/3608 , A61L27/365 , A61L27/46 , A61L27/50 , A61L2400/06 , A61L2430/02 , C08L89/06
摘要： The present invention is directed to bone graft compositions suitable for administration to the body via a cannula, where the compositions contain mineralized collagen particles and a fluid biocompatible carrier having the mineralized collagen particles substantially uniformly distributed there through, which particles contain bound mineralized collagen fibrils substantially uniformly distributed there through and a binder for the fibrils; and to methods of making such particles.
摘要翻译： 本发明涉及适于通过插管向身体施用的骨移植组合物，其中组合物含有矿化胶原颗粒和具有基本均匀分布在其中的矿化胶原颗粒的流体生物相容性载体，该颗粒基本上包含结合的矿化胶原原纤维 均匀地分布在那里，以及用于原纤维的粘合剂; 以及制备这种颗粒的方法。
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公开(公告)号：US20060166331A1
公开(公告)日：2006-07-27
申请号：US10527618
申请日：2003-09-12
CPC分类号： C12N15/101 , B03D3/06 , C12N1/066 , C12N15/1003 , C12N15/1017 , C12Q1/6806 , C12Q2527/119 , C12Q2565/137
摘要： Apparatus and methods are described for pharmaceutical grade manufacture extrachromosomal nucleic acids from cell lysates using flotation to separate and eliminate undesired insoluble cellular debris including chromosomal DNA from the lysates. A gas is introduced to controllably generate bubbles that reduce the density of the cell debris and create a buoyant flocculent phase that can be readily separated from, and thus provide, a substantially clarified fluid lysate phase that is enriched in extrachromosomal DNA but substantially depleted of cellular proteins and chromosomal DNA.
摘要翻译： 对来自细胞裂解物的药物级制造染色体外核酸描述了装置和方法，使用浮选来分离和消除包括来自裂解物的染色体DNA的不期望的不溶性细胞碎片。 引入气体以可控制地产生气泡，其降低细胞碎片的密度并产生浮力絮状相，其可以容易地从基质上澄清的流体裂解物相分离，并且因此提供富含染色体外DNA但基本上耗尽细胞的基本上澄清的流体裂解物相 蛋白质和染色体DNA。
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公开(公告)号：US20110070638A1
公开(公告)日：2011-03-24
申请号：US12850969
申请日：2010-08-05
CPC分类号： C12N15/101 , B03D3/06 , C12N1/066 , C12N15/1003 , C12N15/1017 , C12Q1/6806 , C12Q2527/119 , C12Q2565/137
摘要： Apparatus and methods are described for pharmaceutical grade manufacture extrachromosomal nucleic acids from cell lysates using flotation to separate and eliminate undesired insoluble cellular debris including chromosomal DNA from the lysates. A gas is introduced to controllably generate bubbles that reduce the density of the cell debris and create a buoyant flocculent phase that can be readily separated from, and thus provide, a substantially clarified fluid lysate phase that is enriched in extrachromosomal DNA but substantially depleted of cellular proteins and chromosomal DNA.
摘要翻译： 对来自细胞裂解物的药物级制造染色体外核酸描述了装置和方法，使用浮选来分离和消除包括来自裂解物的染色体DNA的不期望的不溶性细胞碎片。 引入气体以可控制地产生气泡，其降低细胞碎片的密度并产生浮力絮状相，其可以容易地从基质上澄清的流体裂解物相分离，并且因此提供富含染色体外DNA但基本上耗尽细胞的基本上澄清的流体裂解物相 蛋白质和染色体DNA。
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公开(公告)号：US07314746B2
公开(公告)日：2008-01-01
申请号：US10527618
申请日：2003-09-12
CPC分类号： C12N15/101 , B03D3/06 , C12N1/066 , C12N15/1003 , C12N15/1017 , C12Q1/6806 , C12Q2527/119 , C12Q2565/137
摘要： Apparatus and methods are described for pharmaceutical grade manufacture extrachromosomal nucleic acids from cell lysates using flotation to separate and eliminate undesired insoluble cellular debris including chromosomal DNA from the lysates. A gas is introduced to controllably generate bubbles that reduce the density of the cell debris and create a buoyant flocculent phase that can be readily separated from, and thus provide, a substantially clarified fluid lysate phase that is enriched in extrachromosomal DNA but substantially depleted of cellular proteins and chromosomal DNA.
摘要翻译： 对来自细胞裂解物的药物级制造染色体外核酸描述了装置和方法，使用浮选来分离和消除包括来自裂解物的染色体DNA的不期望的不溶性细胞碎片。 引入气体以可控制地产生气泡，其降低细胞碎片的密度并产生浮力絮状相，其可以容易地从基质上澄清的流体裂解物相分离，并且因此提供富含染色体外DNA但基本上耗尽细胞的基本上澄清的流体裂解物相 蛋白质和染色体DNA。
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公开(公告)号：US08158348B2
公开(公告)日：2012-04-17
申请号：US12850969
申请日：2010-08-05
CPC分类号： C12N15/101 , B03D3/06 , C12N1/066 , C12N15/1003 , C12N15/1017 , C12Q1/6806 , C12Q2527/119 , C12Q2565/137
摘要： Apparatus and methods are described for pharmaceutical grade manufacture extrachromosomal nucleic acids from cell lysates using flotation to separate and eliminate undesired insoluble cellular debris including chromosomal DNA from the lysates. A gas is introduced to controllably generate bubbles that reduce the density of the cell debris and create a buoyant flocculent phase that can be readily separated from, and thus provide, a substantially clarified fluid lysate phase that is enriched in extrachromosomal DNA but substantially depleted of cellular proteins and chromosomal DNA.
摘要翻译： 对来自细胞裂解物的药物级制造染色体外核酸描述了装置和方法，使用浮选来分离和消除包括来自裂解物的染色体DNA的不期望的不溶性细胞碎片。 引入气体以可控制地产生气泡，其降低细胞碎片的密度并产生浮力絮状相，其可以容易地从基质上澄清的流体裂解物相分离，并且因此提供富含染色体外DNA但基本上耗尽细胞的基本上澄清的流体裂解物相 蛋白质和染色体DNA。
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